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    ENGINEERED CELLULAR PATHWAYS FOR PROGRAMMED AUTOREGULATION OF DIFFERENTIATION
    3.
    发明申请
    ENGINEERED CELLULAR PATHWAYS FOR PROGRAMMED AUTOREGULATION OF DIFFERENTIATION 有权
    用于编程自动分拣的工程细胞路径

    公开(公告)号：US20100285584A1

    公开(公告)日：2010-11-11

    申请号：US12312197

    申请日：2007-11-01

    申请人： Ron Weiss ,  Ihor Lemischka ,  Priscilla Purnick ,  Christoph Schaniel ,  Miles Miller ,  Patrick Guye

    发明人： Ron Weiss ,  Ihor Lemischka ,  Priscilla Purnick ,  Christoph Schaniel ,  Miles Miller ,  Patrick Guye

    IPC分类号： C12N5/071 ,  C12N15/79

    CPC分类号： C12N15/85 ,  C12N2800/40 ,  C12N2830/002 ,  C12N2830/003 ,  C12N2830/005 ,  C12N2830/006 ,  C12N2830/008 ,  C12N2830/15 ,  C12N2830/55 ,  C12N2830/85

    摘要： The present invention provides compositions and methods for programming mammalian cells to perform desired functions. In particular, the present invention provides compositions and methods for programming stem cells to differentiate into a desired cell type. A quorum sensing systems that regulates the expression of cell fate regulators is introduced into mammalian host cells, such as stem cells. The quorum sensing systems generally comprises vectors that express the components of a bacterial quorum sensing pathway, including proteins which catalyze the synthesis of an autoinducer and a gene encoding a regulatory partner of the autoinducer, and vectors in which genes encoding cell fate regulators are operably linked to a promoter induced by the autoinducer/regulatory partner complex. The system can also comprise vectors in which genes encoding additional cell fate regulators are operably linked to a promoter that is induced by a factor synthesized in response to a first stage of differentiation, so that a second stage of differentiation is triggered.

    摘要翻译： 本发明提供用于编程哺乳动物细胞以实现所需功能的组合物和方法。 特别地，本发明提供了用于编程干细胞以分化成所需细胞类型的组合物和方法。 调节细胞命运调节因子表达的群体感应系统被引入哺乳动物宿主细胞，例如干细胞。 群体感测系统通常包括表达细菌群体感知途径的组分的载体，包括催化自身诱导剂的合成的蛋白质和编码自身诱导剂的调节配偶体的基因以及编码细胞命运调节物的基因可操作地连接的载体 由自动诱导剂/调节配体复合物诱导的启动子。 该系统还可以包含载体，其中编码另外的细胞命运调节子的基因可操作地连接到由响应于第一分化阶段合成的因子诱导的启动子，从而触发第二阶段的分化。

    ANIMALS TRANSGENIC FOR A TETRACYCLINE-REGULATED TRANSCRIPTIONAL INHIBITOR
    7.
    发明申请
    ANIMALS TRANSGENIC FOR A TETRACYCLINE-REGULATED TRANSCRIPTIONAL INHIBITOR 无效
    用于四环素调节的转录抑制剂的动物转基因

    公开(公告)号：US20040003417A1

    公开(公告)日：2004-01-01

    申请号：US09241347

    申请日：1999-02-02

    发明人： HERMANN BUJARD ,  MANFRED GOSSEN

    IPC分类号： A01K067/00 ,  A61K039/00 ,  C12N015/87 ,  C12N005/06

    CPC分类号： A01K67/0275 ,  A01K2217/05 ,  A01K2217/075 ,  A01K2217/20 ,  A01K2227/105 ,  A01K2267/0393 ,  A61K48/00 ,  C07K14/005 ,  C07K14/245 ,  C07K2319/00 ,  C07K2319/09 ,  C07K2319/61 ,  C07K2319/71 ,  C07K2319/80 ,  C12N15/62 ,  C12N15/63 ,  C12N15/635 ,  C12N15/67 ,  C12N15/85 ,  C12N15/8509 ,  C12N2710/16622 ,  C12N2830/003 ,  C12N2830/005 ,  C12N2830/006 ,  C12N2830/32

    摘要： Transgenic animals carrying a transgene comprising a nucleic acid molecule encoding protein useful for regulating the expression of genes in eukaryotic cells and organisms in a highly controlled manner are disclosed. In the regulatory system of the invention, transcription of a tet operator-linked nucleotide sequence is inhibited by a transcriptional inhibitor fuision protein composed of two polypeptides, a first polypeptide which binds to tet operator sequences and a second polypeptide which directly or indirectly inhibits transcription in eukaryotic cells. In various embodiment, the first polypeptide binds to tet operator sequences either: (i) in the absence but not the presence of tetracycline (or an analogue thereof) or (ii) in the presence but not the absence of tetracycline (or an analogue thereof). In a preferred embodiment, the transgene encoding the transcriptional inhibitor fusion protein is integrated at a predetermined location within the chromosome of the transgenic animal.

    摘要翻译： 公开了携带转基因的转基因，其包含编码可用于以高度受控的方式调控真核细胞和生物体中的基因表达的蛋白质的核酸分子。 在本发明的调节系统中，由tet操纵子连接的核苷酸序列的转录受到由两个多肽组成的转录抑制剂功能蛋白的抑制，所述两个多肽是结合tet操纵子序列的第一个多肽和直接或间接抑制转录的第二个多肽 真核细胞。 在各种实施方案中，第一多肽与tet操纵子序列结合：（i）在不存在但不存在四环素（或其类似物）的情况下，或（ii）在存在但不存在四环素（或其类似物）的情况下 ）。 在优选的实施方案中，编码转录抑制剂融合蛋白​​的转基因整合在转基因动物的染色体内的预定位置。