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    EXOGENOUS CONTROL OF MAMMALIAN GENE EXPRESSION THROUGH APTAMER-MEDIATED MODULATION OF POLYADENYLATION
    1.
    发明申请
    EXOGENOUS CONTROL OF MAMMALIAN GENE EXPRESSION THROUGH APTAMER-MEDIATED MODULATION OF POLYADENYLATION 有权

    公开(公告)号：US20250066796A1

    公开(公告)日：2025-02-27

    申请号：US18948183

    申请日：2024-11-14

    Applicant: Baylor College of Medicine

    Inventor： Laising Yen ,  Liming Luo ,  Pei-Wen Chao

    IPC: C12N15/115 ,  A61K48/00 ,  C12N15/67

    Abstract: Embodiments of the disclosure concern the use of expression constructs in which at least one polyA signal is embedded upstream of an expressible transcript, such as within a 5′ UTR for the transcript, for example. In certain embodiments, the polyA signal is comprised within a ligand-binding aptamer, and the binding of the ligand to the aptamer, or lack thereof, dictates the outcome for the expressible transcript. In specific embodiments, absence of the ligand causes the expressed transcript having a polyA in its 5′ UTR to be expressed but then degraded, whereas presence of the ligand causes inhibition of degradation upon expression of the expressible transcript. More than one ligand-binding aptamer may be present on the same expression construct.

    Gene expression cassette capable of initiating translation after completion of transcription for producing high-quality recombinant protein in bacteria
    4.
    发明授权
    Gene expression cassette capable of initiating translation after completion of transcription for producing high-quality recombinant protein in bacteria 有权

    公开(公告)号：US12139715B2

    公开(公告)日：2024-11-12

    申请号：US17253401

    申请日：2018-06-19

    Applicant: SEOUL NATIONAL UNIVERSITY R&DB FOUNDATION

    Inventor： Sang Woo Seo ,  Jina Yang

    IPC: C12N15/67

    Abstract: Provided is a gene expression cassette which, for the production of a high-quality recombinant protein in bacteria, initiates translation after completion of transcription, and more specifically, to a gene expression cassette consisting of a switch capable of stopping translation initiation and a trigger system capable of activating the translation initiation from the switch by re-configuring the transcription-translation coupled system inherent in bacteria such that the translation is initiated only by a full-length mRNA chain template. The transcription and translation in the bacteria can be uncoupled by inserting a trigger sequence activating the translation initiation from the switch into the downstream (3′ terminal) of a target recombinant gene by replacing a natural transcription translation-coupled 5′ UTR with the switch. The productivity of a high-quality full-length recombinant protein can be increased while reducing the costs associated with a purification process.

    MODIFIED SIGNAL PEPTIDE
    5.
    发明申请
    MODIFIED SIGNAL PEPTIDE 有权

    公开(公告)号：US20240368608A1

    公开(公告)日：2024-11-07

    申请号：US18033639

    申请日：2021-10-26

    Applicant: KAO CORPORATION

    Inventor： Hiroki NISHIGUCHI ,  Akihito KAWAHARA

    IPC: C12N15/67 ,  C07K16/00 ,  C12N15/75

    Abstract: A nucleic acid construct includes a polynucleotide encoding a protein of interest and a polynucleotide linked upstream of the polynucleotide and encoding a modified signal peptide. The modified signal peptide includes amino acid residues of X1X2X3X4X5X6 at positions 1 to 6 as counted from the C-terminus, where X1 is G, P, or A; X2 is G, S, or A; and X3 is P, S, or A, provided that X1X2X3 is not GGP; X4 is A; X5 is S, H, or F; and X6 is A and is located at the C-terminus of the modified signal peptide.

    Increasing gene expression
    7.
    发明授权
    Increasing gene expression 有权

    公开(公告)号：US12134772B2

    公开(公告)日：2024-11-05

    申请号：US17276320

    申请日：2019-09-06

    Applicant: Klogenix LLC

    Inventor： Ci-Di Chen ,  Ella Zeldich ,  Carmela Abraham

    IPC: C12N15/67 ,  A61K31/713 ,  C12N9/24 ,  C12N15/113

    Abstract: The present disclosure relates to compositions and methods for modulating gene expression and in particular to compositions and methods for increasing expression of Klotho. In certain examples, the present disclosure provides a method of increasing expression of a Klotho gene in a cell the method comprising administering to the cell a binding molecule that binds to an RNA transcript transcribed from a chromosomal region within or near the Klotho gene, wherein the RNA transcript does not encode a Klotho protein.

    RIBOSOMAL RNA (rRNA) VARIANTS POSSESSING ENHANCED PROTEIN PRODUCTION CAPABILITIES
    10.
    发明公开
    RIBOSOMAL RNA (rRNA) VARIANTS POSSESSING ENHANCED PROTEIN PRODUCTION CAPABILITIES 审中-公开

    公开(公告)号：US20240344062A1

    公开(公告)日：2024-10-17

    申请号：US18682754

    申请日：2022-08-10

    Applicant: THE BROAD INSTITUTE, INC.

    Inventor： Ahmed H. Badran ,  Fan Liu ,  Sinisa Bratulic

    IPC: C12N15/11 ,  C12N15/10 ,  C12N15/67 ,  C12N15/70

    CPC classification number: C12N15/11 ,  C12N15/1058 ,  C12N15/67 ,  C12N15/70

    Abstract: The present disclosure relates to compositions, methods and kits for enhancing ribosomal activities in a host cell, especially improvement of the translation activity of heterologous ribosomes within a host cell. Specifically, the instant disclosure provides a number of evolved rRNA sequences, which were remarkably identified to possess enhanced translation activities, improved orthogonal-ribosome binding site (o-RBS) and orthogonal anti-ribosome binding site (o-antiRBS) sequences, host cells possessing deletion or disruption of ribosome hibernation promoting factor (HPF) that thereby exhibit enhanced propagation of selection phage constructs during (PACE), among other aspects. New transgenic organisms harboring heterologous ribosomes and operons are also provided.

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