公开(公告)号：US12123056B2

公开(公告)日：2024-10-22

申请号：US17153725

申请日：2021-01-20

申请人： Illumina Cambridge Limited

发明人： Jonathan Mark Boutell

IPC分类号： C12P19/34 ,  C12N15/10 ,  C12N15/64 ,  C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/6853

CPC分类号： C12Q1/6874 ,  C12N15/10 ,  C12N15/64 ,  C12Q1/6806 ,  C12Q1/6853 ,  C12Q1/6806 ,  C12Q2525/155 ,  C12Q2533/101 ,  C12Q2535/122 ,  C12Q2565/537 ,  C12Q2565/543

摘要： Presented herein are methods and compositions for concatenating template strands during the bridge amplification process. The methods are useful for surface amplification at improved densities. The methods and compositions provided herein enable creation of clusters that are brighter, but at the same densities as currently achieved using standard cluster amplification.

公开(公告)号：US12104151B2

公开(公告)日：2024-10-01

申请号：US18185048

申请日：2023-03-16

申请人： President and Fellows of Harvard College

发明人： Xiaowei Zhuang ,  Kok-Hao Chen ,  Alistair Boettiger ,  Jeffrey R. Moffitt ,  Siyuan Wang

IPC分类号： C12N15/10 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/6806 ,  C12Q1/6816 ,  C12Q1/6837 ,  C12Q1/6841 ,  C12Q1/6869 ,  G16B25/00 ,  G16B25/20 ,  G16B30/00 ,  G16B40/00 ,  G16B40/10 ,  G16C20/10 ,  G06N7/01

CPC分类号： C12N15/1065 ,  C07H21/02 ,  C07H21/04 ,  C12N15/10 ,  C12N15/1093 ,  C12Q1/6806 ,  C12Q1/6837 ,  G16B25/00 ,  G16B25/20 ,  G16B40/10 ,  G16C20/10 ,  C12Q1/6816 ,  C12Q1/6841 ,  C12Q1/6869 ,  G06N7/01 ,  G16B30/00 ,  G16B40/00 ,  C12Q1/6806 ,  C12Q2521/107 ,  C12Q2525/143 ,  C12Q2525/179 ,  C12Q2537/143 ,  C12Q2565/514 ,  C12Q1/6816 ,  C12Q2525/161 ,  C12Q2537/143 ,  C12Q2563/179 ,  C12Q1/6816 ,  C12Q2525/161 ,  C12Q2537/143 ,  C12Q2563/179 ,  C12Q2565/102

摘要： The present invention generally relates to systems and methods for imaging or determining nucleic acids, for instance, within cells. In some embodiments, the transcriptome of a cell may be determined. Certain embodiments are directed to determining nucleic acids, such as mRNA, within cells at relatively high resolutions. In some embodiments, a plurality of nucleic acid probes may be applied to a sample, and their binding within the sample determined, e.g., using fluorescence, to determine locations of the nucleic acid probes within the sample. In some embodiments, codewords may be based on the binding of the plurality of nucleic acid probes, and in some cases, the codewords may define an error-correcting code to reduce or prevent misidentification of the nucleic acids. In certain cases, a relatively large number of different targets may be identified using a relatively small number of labels, e.g., by using various combinatorial approaches.

公开(公告)号：US20240309427A1

公开(公告)日：2024-09-19

申请号：US18674513

申请日：2024-05-24

申请人： Karius, Inc.

发明人： Timothy A. Blauwkamp ,  Fred Christians ,  Michael Rosen ,  Igor D. Vilfan

IPC分类号： C12Q1/6806 ,  C12N15/10 ,  C12Q1/6848 ,  C12Q1/6869 ,  C12Q1/6876

CPC分类号： C12Q1/6806 ,  C12N15/10 ,  C12N15/1003 ,  C12Q1/6848 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q2523/313

摘要： This disclosure provides methods that are useful for reducing or inactivating contaminant nucleic acids.

公开(公告)号：US20240279308A1

公开(公告)日：2024-08-22

申请号：US18581947

申请日：2024-02-20

申请人： IMMATICS BIOTECHNOLOGIES GMBH

发明人： Dominik MAURER ,  Sebastian BUNK ,  Leonie ALTEN

IPC分类号： C07K14/725 ,  A61K35/12 ,  A61K35/17 ,  A61K35/66 ,  A61K38/00 ,  C07K14/435 ,  C12N15/09 ,  C12N15/10

CPC分类号： C07K14/7051 ,  A61K35/12 ,  A61K35/17 ,  A61K35/66 ,  C07K14/435 ,  C12N15/09 ,  C12N15/10 ,  A61K38/00

摘要： Disclosed are T-cell receptors (TCRs) binding to tumor-associated antigens (TAAs) for targeting cancer cells, T-cells expressing same, methods for producing same, and methods for treating cancers using same. Disclosed are TCRs and their variants that bind to HLA class I or II molecules with a peptide, such as MAG-003 have the amino acid sequence of KVLEHVVRV (SEQ ID NO:1). The description further relates to peptides, proteins, nucleic acids, cells for use in immunotherapeutic methods, the immunotherapy of cancer, and tmor-associated T-cell peptide epitopes, alone or in combination with other tumor-associated peptides that can for example serve as active pharmaceutical ingredients of vaccine compositions that stimulate anti-tumor immune responses, or to stimulate T-cells ex vivo and transfer into patients. Peptides bound to molecules of the major histocompatibility complex (MHC), or peptides as such, can also be targets of antibodies, soluble T-cell receptors, and other binding molecules.

公开(公告)号：US20240263217A1

公开(公告)日：2024-08-08

申请号：US18565849

申请日：2022-06-03

申请人： TRANSLATE BIO, INC.

发明人： Frank DEROSA ,  Anusha DIAS ,  Xiaobo GU

IPC分类号： C12Q1/6825 ,  C12N15/10

CPC分类号： C12Q1/6825 ,  C12N15/10

摘要： The invention relates to a method of quantifying capping efficiency in a sample from an in vitro transcription reaction mixture comprising a plurality of mRNA transcript, characterized by a step of contacting the mRNA transcripts with an oligonucleotide complementary to a sequence of nucleotides in the 5′ untranslated region of the mRNA transcripts to form an mRNA:DNA hybrid between the oligonucleotide and the sequence of nucleotides of the mRNA transcripts in order to release the first five, six, or seven nucleotides of the mRNA transcripts using nuclease (e.g., RNAse H) digestion.

公开(公告)号：US12054705B2

公开(公告)日：2024-08-06

申请号：US17167136

申请日：2021-02-04

申请人： The Regents of the University of California

发明人： Kathleen Collins ,  Heather E. Upton

IPC分类号： C12N15/10 ,  C07K14/435 ,  C12N9/12

CPC分类号： C12N15/10 ,  C07K14/43563 ,  C12N9/1276 ,  C12N2310/344 ,  C12N2310/345 ,  C12N2310/3517

摘要： The invention provides compositions and methods for nucleic acid synthesis, including ordered and continuous complementary DNA (cDNA) synthesis across non-continuous templates using a modified eukaryotic non-long terminal repeat reverse transcriptase (non-LTR RT) protein.

公开(公告)号：US12018250B2

公开(公告)日：2024-06-25

申请号：US16341290

申请日：2017-10-13

申请人： Fred Hutchinson Cancer Center

发明人： Christopher D. Johnston

IPC分类号： C12Q1/689 ,  C12N15/10 ,  C12N15/11 ,  C12N15/113 ,  C12N15/09 ,  C12N15/64 ,  C12N15/74

CPC分类号： C12N15/1027 ,  C12N15/10 ,  C12N15/102 ,  C12N15/11 ,  C12N15/113 ,  C12Q1/689 ,  C12N15/09 ,  C12N15/64 ,  C12N15/74 ,  C12N2310/20 ,  C12N2310/33 ,  C12N2310/333 ,  C12N2330/50 ,  C12Q2600/154 ,  C12Q2600/156

摘要： The present invention features modified polynucleotide sequences that mimic host cell DNA and methods of using such sequences for the genetic engineering of bacteria that are otherwise genetically intractable.

公开(公告)号：US11945854B2

公开(公告)日：2024-04-02

申请号：US17125922

申请日：2020-12-17

申请人： IMMATICS BIOTECHNOLOGIES GMBH

发明人： Dominik Maurer ,  Sebastian Bunk ,  Leonie Alten

IPC分类号： C07K14/725 ,  A61K35/12 ,  A61K35/17 ,  A61K35/66 ,  C07K14/435 ,  C12N15/09 ,  C12N15/10 ,  A61K38/00

CPC分类号： C07K14/7051 ,  A61K35/12 ,  A61K35/17 ,  A61K35/66 ,  C07K14/435 ,  C12N15/09 ,  C12N15/10 ,  A61K38/00

摘要： Disclosed are T-cell receptors (TCRs) binding to tumor-associated antigens (TAAs) for targeting cancer cells, T-cells expressing same, methods for producing same, and methods for treating cancers using same. Disclosed are TCRs and their variants that bind to HLA class I or II molecules with a peptide, such as MAG-003 have the amino acid sequence of KVLEHVVRV (SEQ ID NO:1). The description further relates to peptides, proteins, nucleic acids, cells for use in immunotherapeutic methods, the immunotherapy of cancer, and tumor-associated T-cell peptide epitopes, alone or in combination with other tumor-associated peptides that can for example serve as active pharmaceutical ingredients of vaccine compositions that stimulate anti-tumor immune responses, or to stimulate T-cells ex vivo and transfer into patients. Peptides bound to molecules of the major histocompatibility complex (MHC), or peptides as such, can also be targets of antibodies, soluble T-cell receptors, and other binding molecules.

公开(公告)号：US20240093168A1

公开(公告)日：2024-03-21

申请号：US18260452

申请日：2022-01-04

申请人： KAWASAKI GAKUEN EDUCATIONAL FOUNDATION

发明人： Tatsuo ITO ,  Yurika SHIMIZU

IPC分类号： C12N9/22 ,  A61K31/40 ,  A61K31/505 ,  C12N15/10 ,  C12N15/11

CPC分类号： C12N9/22 ,  A61K31/40 ,  A61K31/505 ,  C12N15/10 ,  C12N15/11 ,  C12N2310/20

摘要： The invention provides a CRISPR-dCas/Cas protein derivative or CRISPR-dCas/Cas protein derivative set derived from a CRISPR-dCas (dead Cas) protein or a CRISPR-Cas protein, as well as a polynucleotide encoding the same, a vector comprising the polynucleotide, a transformant transformed by the vector, a carrier for purifying a target RNA, a method for purifying a target RNA, a method for analyzing an intracellular environment, and a prophylactic or therapeutic agent.

公开(公告)号：US20240035021A1

公开(公告)日：2024-02-01

申请号：US18171578

申请日：2023-02-20

申请人： The J. David Gladstone Institutes ,  The Regents of the University of California

发明人： Leor S. Weinberger ,  Timothy J. Notton

IPC分类号： C12N15/10 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12N15/66

CPC分类号： C12N15/1082 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12N15/66 ,  C12N15/1065 ,  C12N15/10 ,  C12Q2563/185 ,  C12N2800/107 ,  C12N2310/533 ,  C12N2800/90 ,  C12N2800/80 ,  C12N15/867

摘要： Provided are methods and compositions for generating a deletion library, and methods and compositions for generating and identifying a defective interfering particle (DIP). Also provided are transposon cassettes. A subject method can include: inserting a transposon cassette comprising a target sequence for a sequence specific DNA endonuclease into a population of circular target DNAs to generate a population of transposon-inserted circular target DNAs; contacting the population of transposon-inserted circular target DNAs with the sequence specific DNA endonuclease to generate a population of cleaved linear target DNAs; contacting the population of cleaved linear target DNAs with one or more exonucleases to generate a population of deletion DNAs; and circularizing the deletion DNAs to generate a library of circularized deletion DNAs. The population of circular target DNAs can include viral genomic DNA. Also provided ae human immunodeficiency virus (HIV) deletion mutants, e.g., interfering, conditionally replicating, HIV deletion mutants, and related constructs.