公开(公告)号：US20230185971A1

公开(公告)日：2023-06-15

申请号：US17938234

申请日：2022-10-05

Applicant: Twist Bioscience Corporation

Inventor： Bill James PECK

IPC: G06F21/78 ,  C12N15/10

CPC classification number: G06F21/78 ,  C12N15/102 ,  C12N15/1089 ,  C12N15/1027 ,  G06F2212/402 ,  G06F2212/1052

Abstract: Provided herein are compositions, devices, systems and methods for the generation and use of biomolecule-based information for storage. Additionally, devices described herein for de novo synthesis of nucleic acids encoding information related to the original source information may be rigid or flexible material. Further described herein are highly efficient methods for long term data storage with 100% accuracy in the retention of information. Also provided herein are methods and systems for efficient transfer of preselected polynucleotides from a storage structure for reading stored information.

公开(公告)号：US20190241921A1

公开(公告)日：2019-08-08

申请号：US16388722

申请日：2019-04-18

Applicant: Synthetic Genomics, Inc.

Inventor： Daniel G. Gibson ,  Hamilton O. Smith ,  Clyde A. Hutchison ,  Lei Young ,  J. Craig Venter

IPC: C12P19/34 ,  C12N15/66 ,  C12N15/10 ,  C12N15/64

CPC classification number: C12P19/34 ,  C12N15/10 ,  C12N15/1027 ,  C12N15/64 ,  C12N15/66

Abstract: The present invention relates to methods of joining two or more double-stranded (ds) or single-stranded (ss) DNA molecules of interest in vitro, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule of each pair share a region of sequence identity. The method allows the joining of a large number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes. It can be used, e.g., to join synthetically produced sub-fragments of a gene or genome of interest. Kits for performing the method are also disclosed. The methods of joining DNA molecules may be used to generate combinatorial libraries useful to generate, for example, optimal protein expression through codon optimization, gene optimization, and pathway optimization.

公开(公告)号：US20180353621A1

公开(公告)日：2018-12-13

申请号：US15762089

申请日：2016-10-25

Applicant: Quethera Limited

Inventor： Peter WIDDOWSON ,  Keith MARTIN

IPC: A61K48/00 ,  C07K14/48 ,  C12N15/85 ,  C12N15/10 ,  C12N15/62 ,  A61K38/18

CPC classification number: A61K48/0066 ,  A61K38/00 ,  A61K38/185 ,  A61K48/0058 ,  C07K14/48 ,  C12N15/1027 ,  C12N15/62 ,  C12N15/85 ,  C12N15/86 ,  C12N15/8645 ,  C12N2830/008 ,  C12N2840/203

Abstract: The invention provides genetic constructs and recombinant vectors comprising such constructs. The constructs and vectors can be used in gene therapy methods for treating a range of disorders, including glaucoma and deafness, or for promoting nerve regeneration and/or survival.

公开(公告)号：US20180273936A1

公开(公告)日：2018-09-27

申请号：US15921537

申请日：2018-03-14

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： Anthony COX ,  Siyuan CHEN

IPC: C12N15/10 ,  C12Q1/6806

CPC classification number: C12N15/1093 ,  C12N15/1027 ,  C12Q1/6806

Abstract: Disclosed herein are methods for the generation of highly accurate nucleic acid libraries encoding for predetermined variants of a nucleic acid sequence. The nucleic acid sequence may encode for all or part of a reference domain of a CAR. The degree of variation may be complete, resulting in a saturated variant library, or less than complete, resulting in a non-saturating library of variants. The variant nucleic acid libraries described herein may be designed for further processing by transcription or translation. The variant nucleic acid libraries described herein may be designed to generate variant RNA, DNA and/or protein populations. Further provided herein are method for identifying variant species with increased or decreased activities, with applications in regulating biological functions and the design of therapeutics for treatment or reduction of a disease, such as cancer.

公开(公告)号：US20180002707A1

公开(公告)日：2018-01-04

申请号：US15544752

申请日：2016-01-20

Applicant: MAYO FOUNDATION FOR MEDICAL EDUCATION AND RESEARCH

Inventor： Stephen C. Ekker ,  Karl J. Clark ,  Jarryd M. Campbell ,  Chun Hang Alvin Ma

IPC: C12N15/66 ,  C12N9/22

CPC classification number: C12N15/66 ,  C07K2319/00 ,  C07K2319/80 ,  C12N9/22 ,  C12N15/102 ,  C12N15/1027 ,  C12N15/1031 ,  C12N15/64

Abstract: This document provides methods and materials for assembling nucleic acid constructs (e.g., TALENs). For example, methods for assembling TALEs that are rapid, flexible for use in many cloning scaffolds (such as common nuclease and nickase backbones), and achievable with standard molecular biology laboratory tools, thereby making TALEs a more accessible genome system, are provided.

公开(公告)号：US09562250B2

公开(公告)日：2017-02-07

申请号：US14313193

申请日：2014-06-24

Applicant: THE PENN STATE RESEARCH FOUNDATION

Inventor： Stephen J Benkovic ,  Frank Salinas

IPC: C12Q1/68 ,  C12P19/34 ,  C12N15/10 ,  C07H21/02

CPC classification number: C12P19/34 ,  C12N15/1027 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q2521/507 ,  C12Q2521/513 ,  C12Q2527/125

Abstract: A method for replicating and amplifying a target nucleic acid sequence is described. A method of the invention involves the formation of a recombination intermediate without the prior denaturing of a nucleic acid duplex through the use of a recombination factor. The recombination intermediate is treated with a high fidelity polymerase to permit the replication and amplification of the target nucleic acid sequence. In preferred embodiments, the polymerase comprises a polymerase holoenzyme. In further preferred embodiments, the recombination factor is bacteriophage T4 UvsX protein or homologs from other species, and the polymerase holoenzyme comprises a polymerase enzyme, a clamp protein and a clamp loader protein, derived from viral, bacteriophage, prokaryotic, archaebacterial, or eukaryotic systems.

Abstract translation: 描述了用于复制和扩增靶核酸序列的方法。 本发明的方法涉及通过使用重组因子而不具有核酸双链体的先前变性而形成重组中间体。 用高保真聚合酶处理重组中间体以允许靶核酸序列的复制和扩增。 在优选的实施方案中，聚合酶包含聚合酶全酶。 在进一步优选的实施方案中，重组因子是噬菌体T4 UvsX蛋白或来自其他物种的同源物，聚合酶全酶包含衍生自病毒，噬菌体，原核，古细菌或真核系统的聚合酶，钳夹蛋白和夹紧装载蛋白 。

公开(公告)号：US20160237475A1

公开(公告)日：2016-08-18

申请号：US15070529

申请日：2016-03-15

Applicant: THE PENN STATE RESEARCH FOUNDATION

Inventor： Stephen J BENKOVIC ,  Frank Salinas

IPC: C12Q1/68

CPC classification number: C12P19/34 ,  C12N15/1027 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q2521/507 ,  C12Q2521/513 ,  C12Q2527/125

Abstract: A method for replicating and amplifying a target nucleic acid sequence is described. A method of the invention involves the formation of a recombination intermediate without the prior denaturing of a nucleic acid duplex through the use of a recombination factor. The recombination intermediate is treated with a high fidelity polymerase to permit the replication and amplification of the target nucleic acid sequence. In preferred embodiments, the polymerase comprises a polymerase holoenzyme. In further preferred embodiments, the recombination factor is bacteriophage T4 UvsX protein or homologs from other species, and the polymerase holoenzyme comprises a polymerase enzyme, a clamp protein and a clamp loader protein, derived from viral, bacteriophage, prokaryotic, archaebacterial, or eukaryotic systems.

Abstract translation: 描述了用于复制和扩增靶核酸序列的方法。 本发明的方法涉及通过使用重组因子而不具有核酸双链体的先前变性而形成重组中间体。 用高保真聚合酶处理重组中间体以允许靶核酸序列的复制和扩增。 在优选的实施方案中，聚合酶包含聚合酶全酶。 在进一步优选的实施方案中，重组因子是噬菌体T4 UvsX蛋白或来自其他物种的同源物，聚合酶全酶包含衍生自病毒，噬菌体，原核，古细菌或真核系统的聚合酶，钳夹蛋白和夹紧装载蛋白 。

公开(公告)号：US09340826B2

公开(公告)日：2016-05-17

申请号：US14235799

申请日：2012-08-01

Applicant: Duhee Bang ,  Hwangbeom Kim ,  Hyojun Han

Inventor： Duhee Bang ,  Hwangbeom Kim ,  Hyojun Han

IPC: C12Q1/68 ,  C12N15/10

CPC classification number: C12N15/1093 ,  C12N15/1027 ,  C12N15/1031 ,  C12N15/1065 ,  C12Q1/6806

Abstract: Provided is a method of preparing nucleic acid molecules comprising: (a) a step of providing nucleic acid fragments constituting at least a portion of the complete sequence of a target nucleic acid; (b) tagging the nucleic acid fragments with barcode sequences; (c) identifying the sequence of the nucleic acid fragments tagged by the barcode sequences; and (d) recovering desired nucleic acid fragments among the sequence-identified nucleic acid fragments using the barcode sequences.

Abstract translation: 提供了制备核酸分子的方法，包括：（a）提供构成靶核酸的完整序列的至少一部分的核酸片段的步骤; （b）用条形码序列标记核酸片段; （c）鉴定由条形码序列标记的核酸片段的序列; 和（d）使用条形码序列在序列鉴定的核酸片段中回收所需的核酸片段。

公开(公告)号：US09322058B2

公开(公告)日：2016-04-26

申请号：US14268444

申请日：2014-05-02

Applicant: THE PENN STATE RESEARCH FOUNDATION

Inventor： Stephen J Benkovic ,  Frank Salinas

IPC: C12Q1/68 ,  C12N15/10 ,  C12P19/34

CPC classification number: C12P19/34 ,  C12N15/1027 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q2521/507 ,  C12Q2521/513 ,  C12Q2527/125

Abstract: A method for replicating and amplifying a target nucleic acid sequence is described. A method of the invention involves the formation of a recombination intermediate without the prior denaturing of a nucleic acid duplex through the use of a recombination factor. The recombination intermediate is treated with a high fidelity polymerase to permit the replication and amplification of the target nucleic acid sequence. In preferred embodiments, the polymerase comprises a polymerase holoenzyme. In further preferred embodiments, the recombination factor is bacteriophage T4 UvsX protein or homologs from other species, and the polymerase holoenzyme comprises a polymerase enzyme, a clamp protein and a clamp loader protein, derived from viral, bacteriophage, prokaryotic, archaebacterial, or eukaryotic systems.

公开(公告)号：US20150329853A1

公开(公告)日：2015-11-19

申请号：US14653840

申请日：2013-12-19

Applicant: Helge ZIELER

Inventor： Helge ZIELER

IPC: C12N15/10

CPC classification number: C12N15/1082 ,  C07H21/02 ,  C12N15/102 ,  C12N15/1027 ,  C12N15/70 ,  C12N15/81

Abstract: The present invention provides compositions comprising randomized in-frame fusion polynucleotides and methods for introducing them into a host organism to identify desirable phenotypic changes that disrupt or alter existing genetic or biochemical mechanisms or pathways, thus creating novel characteristics of the transformed organism. Methods for using the compositions for increasing diversity within populations of organisms are also presented.

Abstract translation: 本发明提供了包含随机化的帧内融合多核苷酸的组合物和将其引入宿主生物体以鉴定破坏或改变现有遗传或生物化学机制或途径的所需表型变化的方法，从而产生转化生物的新特征。 还提出了使用组合物增加生物群体内多样性的方法。