知搜-全球专利检索

  1. Login
  2. Sign Up

Search Results

34 records (took 0.031 seconds)

    Compositions and methods for creating altered and improved cells and organisms
    1.
    发明授权
    Compositions and methods for creating altered and improved cells and organisms 有权
    用于产生改变和改善的细胞和生物体的组合物和方法

    公开(公告)号：US09200291B2

    公开(公告)日：2015-12-01

    申请号：US14134619

    申请日：2013-12-19

    Applicant: Helge Zieler

    Inventor： Helge Zieler

    IPC: C07H21/02 ,  C12N15/81 ,  C12N15/70 ,  C12N15/10

    CPC classification number: C12N15/1082 ,  C07H21/02 ,  C12N15/102 ,  C12N15/1027 ,  C12N15/70 ,  C12N15/81

    Abstract: The present invention provides compositions comprising randomized in-frame fusion polynucleotides and methods for introducing them into a host organism to identify desirable phenotypic changes that disrupt or alter existing genetic or biochemical mechanisms or pathways, thus creating novel characteristics of the transformed organism. Methods for using the compositions for increasing diversity within populations of organisms are also presented.

    Abstract translation: 本发明提供了包含随机化的帧内融合多核苷酸的组合物和将其引入宿主生物体以鉴定破坏或改变现有遗传或生物化学机制或途径的所需表型变化的方法，从而产生转化生物的新特征。 还提出了使用组合物增加生物群体内多样性的方法。

    IDENTIFICATION OF CENTROMERE SEQUENCES USING CENTROMERE ASSOCIATED PROTEINS AND USES THEREOF
    2.
    发明申请
    IDENTIFICATION OF CENTROMERE SEQUENCES USING CENTROMERE ASSOCIATED PROTEINS AND USES THEREOF 审中-公开
    使用中心相关蛋白鉴定中心序列及其用途

    公开(公告)号：US20120115132A1

    公开(公告)日：2012-05-10

    申请号：US12940931

    申请日：2010-11-05

    Applicant: Gregory P. COPENHAVER ,  Helge Zieler ,  Daphne Preuss

    Inventor： Gregory P. COPENHAVER ,  Helge Zieler ,  Daphne Preuss

    IPC: C12Q1/68 ,  C07H21/00 ,  C07H21/04 ,  C12N1/00

    CPC classification number: C12Q1/6804 ,  C07H21/00 ,  C07H21/04 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q2537/159 ,  C12Q2535/101 ,  C12Q2522/10 ,  C12Q2522/101

    Abstract: The present invention is directed to methods of centromere discovery using centromere-associated proteins in a variety of experimental formats. The methods of the invention can be used on any organism, and include using Cal1, Cbf1, Cbf3, Cbf5, CenH3 (Cenp-A), Cenp-B, Cenp-C, Cenp-D, Cenp-E, Cenp-F, Cenp-G, Cenp-H, Cenp-I, Cenp-K, Cenp-L, Cenp-M, Cenp-N, Cenp-O, Cenp-P, Cenp-Q, Cenp-R, Cenp-S, Cenp-T, Cenp-U, Cenp-V, Cenp-W, Chd1, Chp1, cohesin, condensin, Dnmt3b, Fact, Gcn5p, H2A.Z, Haspin, Hjurp, HP1, Hst4, Ima1, Incep, Ino80, Kms2, Knl-2, Mif2, Mis6, Np95, Pich, Sad1, Scm3, Shugoshin, Sim3, Skp1, Sororin, Survivin, Tas3, ZW10, and homologs thereof to identify centromere sequences. The invention is also directed to artificial chromosomes comprising centromeres made according to the methods of the invention, as well as to cells comprising such artificial chromosomes.

    Abstract translation: 本发明涉及使用各种实验形式的着丝粒相关蛋白的着丝粒发现方法。 本发明的方法可用于任何生物体，包括使用Cal1，Cbf1，Cbf3，Cbf5，CenH3（Cenp-A），Cenp-B，Cenp-C，Cenp-D，Cenp-E，Cenp-F， Cenp-G，Cenp-H，Cenp-I，Cenp-K，Cenp-L，Cenp-M，Cenp-N，Cenp-O，Cenp-P，Cenp-Q，Cenp-R，Cenp-S， T，Cenp-U，Cenp-V，Cenp-W，Chd1，Chp1，凝聚素，凝血酶，Dnmt3b，Fact，Gcn5p，H2A.Z，Haspin，Hjurp，HP1，Hst4，Ima1，Incep，Ino80，Kms2， 2，Mif2，Mis6，Np95，Pich，Sad1，Scm3，Shugoshin，Sim3，Skp1，Sororin，Survivin，Tas3，ZW10及其同源物以鉴定着丝粒序列。 本发明还涉及根据本发明方法制备的包含着丝绒的人造染色体以及包含这种人造染色体的细胞。

    Methods and compositions for creating altered and improved cells and organisms
    6.
    发明授权
    Methods and compositions for creating altered and improved cells and organisms 有权

    公开(公告)号：US10077441B2

    公开(公告)日：2018-09-18

    申请号：US15110712

    申请日：2015-01-09

    Applicant: Helge Zieler

    Inventor： Helge Zieler

    IPC: C12N15/74 ,  C12P21/04 ,  C12P21/06 ,  C07H21/04 ,  C12N15/10

    CPC classification number: C12N15/1079 ,  C07H21/04 ,  C07K2319/00 ,  C12N15/10 ,  C12N15/74

    Abstract: The present invention relates to compositions involving randomized in-frame fusion polynucleotides and their introduction into a host organism to identify desirable phenotypic changes. The present invention further relates to methods of generating these randomized in-frame fusion polynucleotides by introducing randomized in-frame fusion polynucleotides into an organism, selecting for organisms with new or altered phenotypes, re-isolating the randomized in-frame fusion polynucleotides from the selected organisms, re-assembling the constituent polynucleotides of the re-isolated randomized in-frame fusion polynucleotides into new collections of randomized in-frame fusion polynucleotides, and repeating the selection for organisms with new or altered phenotypes.

    CENTROMERE SEQUENCES AND MINICHROMOSOMES
    9.
    发明申请
    CENTROMERE SEQUENCES AND MINICHROMOSOMES 失效
    中心序列和微型体

    公开(公告)号：US20100297769A1

    公开(公告)日：2010-11-25

    申请号：US12531287

    申请日：2008-03-14

    Applicant: Helge Zieler ,  Gary W. Rudgers ,  Gregory P. Copenhaver ,  Daphne Preuss ,  Michael H. Pauley

    Inventor： Helge Zieler ,  Gary W. Rudgers ,  Gregory P. Copenhaver ,  Daphne Preuss ,  Michael H. Pauley

    IPC: C12N5/10 ,  C07H21/04

    CPC classification number: C12N15/82 ,  C12N15/8201

    Abstract: The invention is generally related to methods of generating plants transformed with novel autonomous mini-chromosomes. Mini-chromosomes with novel compositions and structures are used to transform plants cells which are in turn used to generate the plant. Methods for generating the plant include methods for delivering the mini-chromosome into plant cell to transform the cell, methods for selecting the transformed cell, and methods for isolating plants transformed with the mini-chromosome. Plants generated in the present invention contain novel genes introduced into their genome by integration into existing chromosomes.

    Abstract translation: 本发明通常涉及用新型自主小型染色体转化的植物的方法。 具有新组合物和结构的微型染色体用于转化依次用于产生植物的植物细胞。 用于产生植物的方法包括将微型染色体递送到植物细胞以转化细胞的方法，用于选择转化细胞的方法，以及分离用微型染色体转化的植物的方法。 本发明产生的植物含有通过整合入现有染色体而引入其基因组的新基因。

Patent Agency Ranking