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公开(公告)号：US20190085014A1
公开(公告)日：2019-03-21
申请号：US16149114
申请日：2018-10-01
发明人： Jingyue Ju , Zengmin Li , John Robert Edwards , Yasuhiro Itagaki
IPC分类号： C07H19/14 , C12Q1/6869 , C07H21/00 , C12Q1/686 , C12Q1/6872 , C12Q1/6876 , C07H19/10 , C12Q1/68 , C12Q1/6874 , C40B40/00
CPC分类号： C12Q1/6869 , C07B2200/11 , C07H19/10 , C07H19/14 , C07H21/00 , C12Q1/68 , C12Q1/686 , C12Q1/6872 , C12Q1/6874 , C12Q1/6876 , C12Q2525/117 , C12Q2525/186 , C12Q2535/101 , C12Q2535/122 , C12Q2563/107 , C12Q2565/501 , C40B40/00
摘要： This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.
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公开(公告)号：US20190031705A1
公开(公告)日：2019-01-31
申请号：US16150185
申请日：2018-10-02
发明人： Jingyue Ju , Zengmin Li , John Robert Edwards , Yasuhiro Itagaki
IPC分类号： C07H19/14 , C12Q1/6869 , C07H19/10 , C12Q1/6874 , C12Q1/6876 , C12Q1/6872 , C12Q1/68 , C12Q1/686
CPC分类号： C12Q1/6869 , C07B2200/11 , C07H19/10 , C07H19/14 , C07H21/00 , C12Q1/68 , C12Q1/686 , C12Q1/6872 , C12Q1/6874 , C12Q1/6876 , C12Q2525/117 , C12Q2525/186 , C12Q2535/101 , C12Q2535/122 , C12Q2563/107 , C12Q2565/501 , C40B40/00
摘要： This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.
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公开(公告)号：US20180276335A1
公开(公告)日：2018-09-27
申请号：US15921812
申请日：2018-03-15
IPC分类号： G06F19/18 , C12Q1/6858 , C12Q1/6869
CPC分类号： G16B20/00 , C12Q1/6858 , C12Q1/6869 , C12Q2535/101 , C12Q2537/143 , C12Q2539/105
摘要： Systems and method for identifying long deletions can obtain sequencing information for a plurality of amplicons in and around a potential region from a nucleic acid sample. The sequencing information can include a plurality of reads that can be mapped to a reference sequence. Using information, such as where reads map to a reference sequence and relative abundance of reads for the amplicons, structural variants can be identified and a determination can be made if the nucleic acid sample is homozygous or heterozygous for the structural variant.
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公开(公告)号：US09953130B2
公开(公告)日：2018-04-24
申请号：US14503461
申请日：2014-10-01
CPC分类号： G06F19/18 , C12Q1/6858 , C12Q1/6869 , C12Q2535/101 , C12Q2537/143 , C12Q2539/105
摘要： Systems and method for identifying long deletions can obtain sequencing information for a plurality of amplicons in and around a potential region from a nucleic acid sample. The sequencing information can include a plurality of reads that can be mapped to a reference sequence. Using information, such as where reads map to a reference sequence and relative abundance of reads for the amplicons, structural variants can be identified and a determination can be made if the nucleic acid sample is homozygous or heterozygous for the structural variant.
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公开(公告)号：US20180010180A1
公开(公告)日：2018-01-11
申请号：US15631414
申请日：2017-06-23
发明人： Wei ZHOU , Rui MEI , Hajime MATSUZAKI
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q1/6874 , C12Q2525/185 , C12Q2527/113 , C12Q2535/122 , C12Q2521/501 , C12Q2525/186 , C12Q2535/101
摘要： The present invention provides methods and systems for sequencing long nucleic acid fragment. The present invention also provides a method of sequencing a target polynucleotide with fewer probes. Further, the present invention provides a method of sequencing a target polynucleotide with longer reads. Locus-specific, ligation-assisted sequencing/genotyping method and ligation-captured sequencing method are also provided in the present invention. The methods of the present invention allow low-cost, high-throughput and accurate sequencing of nucleic acids.
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公开(公告)号：US09725480B2
公开(公告)日：2017-08-08
申请号：US15167917
申请日：2016-05-27
发明人： Jingyue Ju , Zengmin Li , John Robert Edwards , Yasuhiro Itagaki
CPC分类号： C07H19/14 , C07B2200/11 , C07H19/10 , C07H21/00 , C12Q1/68 , C12Q1/686 , C12Q1/6869 , C12Q1/6872 , C12Q1/6874 , C12Q1/6876 , C12Q2525/117 , C12Q2535/101 , C12Q2535/122 , C12Q2563/107 , C40B40/00 , C12Q2565/501 , C12Q2525/186
摘要： This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analog after the nucleotide analog is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogs which comprise unique labels attached to the nucleotide analog through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.
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公开(公告)号：US20170029884A1
公开(公告)日：2017-02-02
申请号：US15214737
申请日：2016-07-20
发明人： Steven Gordon , Jerzy Olejnik
CPC分类号： C07H19/00 , C12Q1/6869 , C12Q1/6874 , C12Q2535/101 , G01N21/05 , G01N21/6428 , G01N21/645 , G01N2021/0346 , G01N2021/058 , G01N2021/6417 , G01N2021/6419 , G01N2021/6421 , G01N2021/6439 , G01N2021/6441 , G01N2021/6471 , G01N2201/062 , G01N2201/0627 , G01N2201/12
摘要： The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.
摘要翻译： 本发明提供了方法和组合物，包括但不限于使用例如通过合成方法的测序获得的数据来确定核苷酸序列中核酸的身份的算法，计算机可读介质，计算机程序，装置和系统。 本发明的方法包括校正核苷酸测序期间遇到的一种或多种现象，例如通过合成方法进行测序。 这些现象包括但不限于序列引导，序列滞后，光谱串扰以及由照明和/或滤波器响应变化引起的噪声。
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公开(公告)号：US09434989B2
公开(公告)日：2016-09-06
申请号：US14691042
申请日：2015-04-20
发明人： Steven Gordon , Jerzy Olejnik
CPC分类号： C07H19/00 , C12Q1/6869 , C12Q1/6874 , C12Q2535/101 , G01N21/05 , G01N21/6428 , G01N21/645 , G01N2021/0346 , G01N2021/058 , G01N2021/6417 , G01N2021/6419 , G01N2021/6421 , G01N2021/6439 , G01N2021/6441 , G01N2021/6471 , G01N2201/062 , G01N2201/0627 , G01N2201/12
摘要： The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.
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9.发明申请公开(公告)号：US20160194697A1
公开(公告)日：2016-07-07
申请号：US14925656
申请日：2015-10-28
发明人： Linda G. Lee , Sam L. Woo , Congcong Ma , Richard T. Reel , Mark F. Oldham , David M. Cox , Benjamin G. Schroeder , Jon M. Sorenson , Willy Wiyatno
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , B01J2219/00353 , B01J2219/0036 , B01J2219/00364 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00626 , B01J2219/00637 , B01J2219/00653 , B01J2219/00657 , B01J2219/00659 , B01L3/0293 , B01L3/502715 , B01L3/502784 , B01L7/52 , B01L2200/0673 , B01L2200/10 , B01L2300/0864 , B01L2300/0867 , B01L2400/0421 , B01L2400/0487 , C12Q1/6806 , C12Q1/6874 , F15C5/00 , F16K99/0001 , F16K99/0011 , F16K99/0013 , F16K2099/0084 , G01N1/14 , G01N27/44743 , G01N27/44769 , G01N35/08 , Y10T137/0318 , Y10T137/4259 , Y10T137/85978 , Y10T137/85986 , Y10T137/86863 , Y10T436/2575 , C12Q2535/101
摘要： Various embodiments of the teachings relate to a system or method for sample preparation or analysis in biochemical or molecular biology procedures. The sample preparation can involve small volume processed in discrete portions or segments or slugs, herein referred to as discrete volumes. A molecular biology procedure can be nucleic acid analysis. Nucleic acid analysis can be an integrated DNA amplification/DNA sequencing procedure.
摘要翻译： 本发明的各种实施方案涉及生物化学或分子生物学程序中用于样品制备或分析的系统或方法。 样品制备可以涉及在离散部分或段或块中的小体积处理，在本文中称为离散体积。 分子生物学程序可以是核酸分析。 核酸分析可以是一个综合的DNA扩增/ DNA测序程序。
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10.发明申请DNA SEQUENCING WITH NON-FLUORESCENT NUCLEOTIDE REVERSIBLE TERMINATORS AND CLEAVABLE LABEL MODIFIED NUCLEOTIDE TERMINATORS 审中-公开使用非荧光核苷酸可逆终止子和可清除标签的DNA序列修饰核酸酶终止子公开(公告)号：US20160024574A1
公开(公告)日：2016-01-28
申请号：US14820254
申请日：2015-08-06
申请人： Jingyue Ju , Dae Hyun Kim , Jia Guo , Qinglin Meng , Zengmin Li , Huanyan Cao
发明人： Jingyue Ju , Dae Hyun Kim , Jia Guo , Qinglin Meng , Zengmin Li , Huanyan Cao
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , A61K31/70 , C07H19/04 , C12Q1/6869 , C12Q1/6876 , C12Q2523/107 , C12Q2523/319 , C12Q2535/101 , C12Q2535/113 , C12Q2563/107
摘要： This invention provides a process for sequencing nucleic acids using 3′ modified deoxynucleotide analogues or 3′ modified deoxyinosine triphosphate analogues, and 3′ modified dideoxynucleotide analogues having a detectable marker attached to a base thereof.
摘要翻译： 本发明提供了使用3'修饰的脱氧核苷酸类似物或3'修饰的脱氧蛋白三磷酸酯类似物和3'修饰的双脱氧核苷酸类似物的序列来测序核酸的方法，其具有连接到其碱基上的可检测标记。
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