公开(公告)号：US11865508B2

公开(公告)日：2024-01-09

申请号：US17008425

申请日：2020-08-31

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Neal Woodbury ,  Zhang-Gong Zhao

IPC: B01J19/00 ,  C07K1/04 ,  C40B50/18 ,  C40B80/00

CPC classification number: B01J19/0046 ,  C07K1/047 ,  C40B50/18 ,  C40B80/00 ,  B01J2219/00432 ,  B01J2219/00596 ,  B01J2219/00626 ,  B01J2219/00659 ,  B01J2219/00675 ,  B01J2219/00725

Abstract: Described herein are in situ synthesized arrays and methods of making the them, wherein array signal sensitivity and robustness is enhanced by carrying out conditioning steps and/or generating linkers during synthesis. An array comprises a surface with a collection of features, wherein the features comprise molecules or polymers attached to the surface. In certain embodiments of the invention, carrying out conditioning steps during array synthesis can yield arrays with improved signal. In other embodiments, linkers are synthesized on the array surface prior to synthesis of functional molecules, wherein increasing linker length can correspond to an improvement in the signal generated by the array.

公开(公告)号：US11654411B2

公开(公告)日：2023-05-23

申请号：US17205263

申请日：2021-03-18

Applicant: Illumina Cambridge Limited

Inventor： Mark Edward Brennan Smith ,  Andrea Sabot ,  Isabelle Marie Julia Rasolonjatovo ,  Jean-Ernest Sohna Sohna ,  Adrian Martin Horgan ,  Harold Philip Swerdlow

IPC: B01J19/00 ,  C40B50/18 ,  C12Q1/6834 ,  C40B40/06 ,  C12Q1/6876 ,  C12Q1/6806 ,  C12Q1/6837 ,  C08F222/38

CPC classification number: B01J19/0046 ,  C08F222/38 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6837 ,  C12Q1/6876 ,  C40B40/06 ,  C40B50/18 ,  B01J2219/0061 ,  B01J2219/0072 ,  B01J2219/00351 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00639 ,  B01J2219/00641 ,  B01J2219/00716 ,  B01J2219/00722 ,  Y10T428/265

Abstract: Methods and compositions are disclosed relating to the localization of nucleic acids to arrays such as silane-free arrays, and of sequencing the nucleic acids localized thereby.

公开(公告)号：US20180149611A1

公开(公告)日：2018-05-31

申请号：US15823610

申请日：2017-11-28

Applicant: Caduceus Biotechnology Inc. ,  HELIOS BIOELECTRONICS INC.

Inventor： Golden Tiao ,  Rung-Ywan Tsai ,  Wen-Yih Chen

IPC: G01N27/327 ,  G01N27/406 ,  G01N27/417

CPC classification number: G01N27/3275 ,  B01J2219/00605 ,  B01J2219/00626 ,  B01J2219/00653 ,  G01N27/406 ,  G01N27/4145 ,  G01N27/417 ,  G01N33/54353 ,  G01N33/54373

Abstract: A biosensors and a method of forming a probe on a solid surface of a biosensor are disclosed and the method includes the following steps. A unit (a nucleotide or an amino acid) capped with a protecting group is covalently bonded on the solid surface of one of a plurality of sensor cells of the biosensor. At least one cycle of the following steps is performed until a desired number of units is formed: irradiating the one of the plurality of sensor cells, so as to remove the protecting group of the unit; and binding a unit capped with the protecting group to the de-protected unit. The one of the plurality of sensor cells is irradiated to remove the protecting group.

公开(公告)号：US20180104663A1

公开(公告)日：2018-04-19

申请号：US15622661

申请日：2017-06-14

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Alexei Finski ,  Gavin MacBeath

IPC: B01J19/00 ,  G01N33/50 ,  G01N33/68

CPC classification number: B01J19/0046 ,  B01J2219/00596 ,  B01J2219/0061 ,  B01J2219/00626 ,  B01J2219/00691 ,  B01J2219/00695 ,  B01J2219/00702 ,  B01J2219/00725 ,  G01N33/5091 ,  G01N33/6803 ,  G01N33/6848

Abstract: The invention provides a method for the isolation of a single cell embedded in a tissue while preserving the state of molecules of the cell, and therefore allows for transformation of a single target cell in live tissue into a format that can be evaluated using analytical methods.

公开(公告)号：US20170369936A1

公开(公告)日：2017-12-28

申请号：US15425882

申请日：2017-02-06

Applicant: Kerry B. Gunning ,  Mark Aaron Behlke

Inventor： Kerry B. Gunning ,  Mark Aaron Behlke

IPC: C12Q1/68 ,  C12P19/34 ,  B01J19/00

CPC classification number: C12Q1/6837 ,  B01J19/0046 ,  B01J2219/0059 ,  B01J2219/00608 ,  B01J2219/00626 ,  B01J2219/00722 ,  C12P19/34 ,  C12Q1/6806 ,  Y10T436/108331 ,  C12Q2525/125 ,  C12Q2533/101 ,  C12Q2521/319

Abstract: The invention provides a novel array method for nucleic acid sequence detection with improved specificity which allows for detection of genetic variation, from simple SNPs (where the variation occurs at a fixed position and is of limited allelic number) to more complex sequence variation patterns (such as with multigene families or multiple genetic strains of an organism where the sequence variation between the individual members is neither fixed nor consistent). The array is comprised of short, synthetic oligonucleotide probes attached to a solid surface which are hybridized to single-stranded targets. Single stranded targets can be produced using a method that employs primers modified on the 5′ end to prohibit degradation by a 5′-exonuclease that is introduced to degrade the unprotected strand. The invention further provides for printing buffers/solutions for the immobilization of oligonucleotide probes to an array surface. The invention also provides hybridization and wash buffers and conditions to maximize hybridization specificity and signal intensity, and reduce hybridization times.

公开(公告)号：US20170354967A1

公开(公告)日：2017-12-14

申请号：US15679111

申请日：2017-08-16

Applicant: CapitalBio Corporation ,  Tsinghua University

Inventor： Shengce TAO ,  Jing CHENG ,  Xuemei MA ,  Qiong ZHANG ,  Yuxiang ZHOU

IPC: B01L3/00 ,  C12Q1/68 ,  C40B40/06 ,  B01L7/00 ,  C40B60/14

CPC classification number: B01L3/508 ,  B01J2219/00385 ,  B01J2219/00527 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00722 ,  B01L7/52 ,  B01L2300/0636 ,  B01L2300/0822 ,  B01L2300/163 ,  C12Q1/6837 ,  C40B40/06 ,  C40B60/14 ,  C12Q2547/101 ,  C12Q2531/131

Abstract: This invention relates generally to the field of nucleic acid detection. In particular, the invention provides a lab-on-chip system for analyzing a nucleic acid, which system comprises, inter alia, controllably closed space, and a target nucleic acid can be prepared and/or amplified, and hybridized to a nucleic acid probe, and the hybridization signal can be acquired if desirable, in the controllably closed space without any material exchange between the controllably closed space and the outside environment. Methods for analyzing a nucleic acid using the lab-on-chip system is also provided.

公开(公告)号：US20170240886A1

公开(公告)日：2017-08-24

申请号：US15440293

申请日：2017-02-23

Applicant: GINKGO BIOWORKS, INC.

Inventor： Andrew V. Oleinikov

IPC: C12N15/10

CPC classification number: C12N15/1068 ,  B01J19/0046 ,  B01J2219/00378 ,  B01J2219/00385 ,  B01J2219/00432 ,  B01J2219/00454 ,  B01J2219/005 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/00626 ,  B01J2219/00639 ,  B01J2219/00641 ,  B01J2219/00659 ,  B01J2219/00675 ,  B01J2219/00677 ,  B01J2219/00689 ,  B01J2219/00713 ,  B01J2219/00722 ,  B82Y30/00 ,  C12Q1/6837 ,  C40B40/06 ,  C40B50/14 ,  C40B80/00

Abstract: There is disclosed a process for in vitro synthesis and assembly of long, gene-length polynucleotides based upon assembly of multiple shorter oligonucleotides synthesized in situ on a microarray platform. Specifically, there is disclosed a process for in situ synthesis of oligonucleotide fragments on a solid phase microarray platform and subsequent, “on device” assembly of larger polynucleotides composed of a plurality of shorter oligonucleotide fragments.

公开(公告)号：US09675954B2

公开(公告)日：2017-06-13

申请号：US14402693

申请日：2013-05-23

Applicant: ALBERT-LUDWIGS-UNIVERSITAET FREIBURG

Inventor： Guenter Roth

IPC: B01J19/00 ,  C40B20/02

CPC classification number: B01J19/0046 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00533 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/00626 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00693 ,  B01J2219/00704 ,  B01J2219/00722 ,  B01J2219/00725

Abstract: The invention relates to a method for producing a microarray, wherein the production of this array is detected in real time from the accumulation of the product molecules being produced. The invention further relates to a microarray produced by this method, and to a device for the real-time detection of molecular accumulations on an array surface during the production of microarrays.

公开(公告)号：US09623394B2

公开(公告)日：2017-04-18

申请号：US13983449

申请日：2012-02-03

Applicant: Günter Roth ,  Jürgen Burger

Inventor： Günter Roth ,  Jürgen Burger

IPC: B01J19/00 ,  B01L3/00 ,  B82Y30/00

CPC classification number: B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00337 ,  B01J2219/00495 ,  B01J2219/00529 ,  B01J2219/00533 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00675 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L3/508 ,  B01L2200/0689 ,  B01L2300/0636 ,  B01L2300/0822 ,  B82Y30/00

Abstract: The invention relates to a device and a method for the generation of molecular microarrays. The invention relates therefore to a universal approach for the generation of protein microarrays, DNA microarrays and RNA microarrays (in general nucleic acid microarrays), by production of an output molecule from a template molecule microarray via enzymatic or chemical processes and transfer of the output molecule onto the desired molecular microarray.

公开(公告)号：US09599611B2

公开(公告)日：2017-03-21

申请号：US11912565

申请日：2006-04-25

Applicant: M. Selim Unlu ,  David A. Bergstein ,  Michael F. Ruane ,  Bennett B. Goldberg

Inventor： M. Selim Unlu ,  David A. Bergstein ,  Michael F. Ruane ,  Bennett B. Goldberg

IPC: C40B30/04 ,  G01N33/543 ,  C12Q1/68 ,  G01N21/45 ,  G01N21/75 ,  G02B21/34 ,  C40B40/06 ,  G01N21/77

CPC classification number: G01N21/77 ,  B01J2219/00378 ,  B01J2219/00387 ,  B01J2219/00432 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00707 ,  B01J2219/00722 ,  B01J2219/00725 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6837 ,  G01N21/45 ,  G01N21/75 ,  G01N33/54373 ,  G01N2021/7779 ,  G01N2201/061 ,  G02B21/34 ,  C12Q2565/619

Abstract: This disclosure provides methods and devices for the label-free detection of target molecules of interest. The principles of the disclosure are particularly applicable to the detection of biological molecules (e.g., DNA, RNA, and protein) using standard SiO2-based microarray technology.