公开(公告)号：US12227790B2

公开(公告)日：2025-02-18

申请号：US17822521

申请日：2022-08-26

Applicant: CAPITALBIO CORPORATION ,  TSINGHUA UNIVERSITY

Inventor： He Yan ,  Wanli Xing ,  Ying Lu

IPC: C12N15/00 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6844

Abstract: A method of enriching nucleic acid, including mixing a sample with a solid phase extraction material; and separating the solid phase extraction material; wherein the solid phase extraction material is glass beads or magnetic beads modified with reduced graphene oxide. Also disclosed is a method of detecting nucleic acid, including mixing a nucleic acid sample enriched by the method above with a probe; and amplifying and detecting an amplification product by electrophoresis.

公开(公告)号：US20210332348A1

公开(公告)日：2021-10-28

申请号：US17237495

申请日：2021-04-22

Applicant: Capitalbio Corporation ,  West China Hospital of Sichuan University

Inventor： Xiang CHEN ,  Lei WANG ,  Su LI ,  Longtang ZHENG ,  Yanan WANG ,  Fei WEN ,  Juan XIN ,  Wentian ZHANG ,  Jing CHENG

IPC: C12N15/10 ,  C12Q1/6806

Abstract: A nucleic acid extraction composition, reagents and kits containing the same and uses thereof. Provided is a nucleic acid extraction and purification reagent free of volatile organic solvents, which prevents the damage of volatile organic solvents to people and greatly improves the timeliness of nucleic acid extraction and purification, making the operation extremely simple, and the nucleic acid can be obtained within 10 minutes. The obtained nucleic acid may be used for biological reactions such as PCR, NASBA, LAMP and RPA. Moreover, the reagents of the present disclosure may be used to extract nucleic acids of cells, bacteria, fungi, DNA viruses and RNA viruses from various samples such as blood, throat swab preserving fluid, saliva, urine, sputum, excrement and the like, and very suitable for clinical and scientific research uses.

公开(公告)号：US20210322982A1

公开(公告)日：2021-10-21

申请号：US17121337

申请日：2020-12-14

Applicant: Capitalbio Corporation

Inventor： Longtang Zheng ,  Lei Wang ,  Liang Bai ,  Xinying Zhou ,  Fei Wen ,  Yanan Wang ,  Xiang Chen ,  Tengfei Guo ,  Baolian Li ,  Li Ma ,  Jing Cheng

IPC: B01L3/00

Abstract: A sample extraction chip and a biological reaction device are disclosed according to the present disclosure. The sample extraction chip includes a chip body and a sample extraction module provided on the chip body, the sample extraction module includes a sample-loading lysis unit, a liquid release-control unit, an extraction unit, a liquid switch-control unit, a liquid collection unit and a sample collection unit, which are connected through flow channels in a sequence of extraction. The liquid release-control unit is configured to store and release liquid reagents, and the liquid switch-control unit is configured to switch between communication of the liquid collection unit and the extraction unit and communication of the sample collection unit and the extraction unit. The sample collection unit includes a front collection portion and a rear collection portion which are both in communication with the liquid switch-control unit.

公开(公告)号：US10099218B2

公开(公告)日：2018-10-16

申请号：US15356461

申请日：2016-11-18

Applicant: CapitalBio Corporation

Inventor： Lei Wang ,  Xinying Zhou ,  Li Ma ,  Juan Xin ,  Mingxian Lin ,  Jinhai Feng

IPC: B01L3/00 ,  B81C3/00

Abstract: In one aspect, disclosed herein are methods for packaging biochips, including microfluidic chips. The method can comprise bonding a substrate and a cover slide, packaging the bonded chip, creating a vacuum in the package, and applying a pressure on the packaged chip. The method is particularly useful for minimizing bubble formation during low-cost and mass production of microfluidic chips.

公开(公告)号：US20180201998A1

公开(公告)日：2018-07-19

申请号：US15744719

申请日：2015-07-14

Applicant: CapitalBio Corporation ,  Tsinghua University

Inventor： Guangxin XIANG ,  Xuezhong LIU ,  Xingping CHAI ,  Wanli XING ,  Jing CHENG

IPC: C12Q1/6883

CPC classification number: C12Q1/6883 ,  C12Q1/6827 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2563/103 ,  C12Q2563/149 ,  C12Q2565/519

Abstract: In one aspect, a kit for detection of genetic gene mutation is provided, which is used for detecting nine deafness gene mutations of Caucasian populations, including GJB2 (c.35delG, c.167delT, c.132G>C, and c.269T>C), GJB6 (c.del309kb), SLC26A4 (c.707T>C and c.1246A>C), 12S rRNA (m.1555A>G and m.7444G>A). In another aspect, a method is provided, which method comprises labeling a target molecule with a luminophore, coupling the target molecule to a particle, and binding to a probe molecule on microarray. In some aspects, this technology, with high sensitivity, enables the detection and interpretation of molecular interactions in an efficient way.

公开(公告)号：US20170285050A1

公开(公告)日：2017-10-05

申请号：US15511216

申请日：2015-09-25

Applicant: CapitalBio Corporation

Inventor： Yuan XUE ,  Guanbin ZHANG ,  Cheng CHEN ,  Kaijun ZHAO ,  Yu DONG ,  Jing CHENG

IPC: G01N35/00 ,  G01N21/25 ,  G01N21/78

CPC classification number: G01N35/00069 ,  G01N21/251 ,  G01N21/78 ,  G01N2035/00158 ,  G01N2035/00306

Abstract: In some aspects, the present disclosure provides a biochip detecting device that comprises a first shell (2) connect to a second shell (1), and a rotatable support frame (5) of biochip possessing a detecting zone that holds one or more biochips, and a detector (7).

公开(公告)号：US09353410B2

公开(公告)日：2016-05-31

申请号：US14340368

申请日：2014-07-24

Applicant: CapitalBio Corporation ,  Tsinghua University

Inventor： Hang Li ,  Jinglin Zhou ,  Dong Wang

IPC: G01N35/04 ,  C12Q1/68 ,  G01N35/00 ,  G01N27/74 ,  B03C1/06

CPC classification number: C12Q1/686 ,  B03C1/06 ,  G01N27/745 ,  G01N35/0098

Abstract: In some aspects, the present disclosure provides a magnetic rack comprising a lateral movement structure and/or a longitudinal movement structure. In particular embodiments, the lateral movement structure and/or longitudinal movement structure move one or more magnets to or away from a reaction tube, in order to control the magnetic forces exerted on microbeads in the reaction tube. The microbeads are attracted onto the reaction tube wall, thereby facilitating separation of the microbeads from a solution in the tube. In particular embodiments, the magnetic rack is used to extract or purify nucleic acid from a sample.

Abstract translation: 在一些方面，本公开提供了一种包括横向运动结构和/或纵向运动结构的磁性架。 在特定实施例中，横向移动结构和/或纵向移动结构将一个或多个磁体移动到或远离反应管，以便控制施加在反应管中的微珠上的磁力。 微珠被吸引到反应管壁上，从而有助于将微珠与管中的溶液分离。 在具体实施方案中，磁性架用于从样品中提取或纯化核酸。

公开(公告)号：US20140285215A1

公开(公告)日：2014-09-25

申请号：US14171757

申请日：2014-02-03

Applicant: CAPITALBIO CORPORATION

Inventor： Lei Wang ,  Keith Mitchelson ,  He Wang ,  Jing Cheng

IPC: G01N27/06 ,  G01N33/487 ,  G01N27/22

CPC classification number: G01N27/06 ,  G01N27/221 ,  G01N33/48707 ,  G01N33/48728 ,  G01N33/5438

Abstract: Systems, apparatus and methods for designing and operating a cell-electrode impedance sensor to detect chemical and biological samples, including biological cells. In one implementation, a method of designing a cell-electrode impedance sensor includes determining a cell free cell-electrode impedance and a cell covered cell-electrode impedance based on a design model for the cell-electrode impedance sensor, wherein the design model is based on one or more factors, the factors including properties and elements of a cell-electrode impedance measurement system, using the cell free cell-electrode impedance and the cell covered cell-electrode impedance to obtain a sensor sensitivity of the cell-electrode impedance measurement system, and choosing one or more design parameters of the cell-electrode impedance sensor in the cell-electrode impedance measurement system to maximize the sensor sensitivity.

Abstract translation: 用于设计和操作细胞电极阻抗传感器以检测包括生物细胞在内的化学和生物样品的系统，装置和方法。 在一个实施方式中，设计电池电极阻抗传感器的方法包括基于用于电池电极阻抗传感器的设计模型来确定无电池单元电极阻抗和电池覆盖的电池电极阻抗，其中设计模型基于 关于一个或多个因素，包括电池电极阻抗测量系统的特性和元件的因素，使用无电池电池电阻和电池覆盖的电池 - 电极阻抗来获得电池电极阻抗测量系统的传感器灵敏度 ，并且在单元电极阻抗测量系统中选择电池电极阻抗传感器的一个或多个设计参数以最大化传感器灵敏度。

公开(公告)号：US20140128281A1

公开(公告)日：2014-05-08

申请号：US14073745

申请日：2013-11-06

Applicant: Tsinghua University ,  CapitalBio Corporation

Inventor： Guanbin ZHANG ,  Kaijun ZHAO ,  Tao SHENG ,  Shuang AN ,  Tao GUO ,  Jia WANG ,  Wanli XING ,  Jing CHENG

IPC: B01J19/00

CPC classification number: B01J19/0046

Abstract: The present disclosure provides a cover sheet for a microarray reaction device. In one aspect, the present cover sheet or device ensures the reaction units/volumes are stable and/or consistent among assay samples and assay runs, allowing samples (e.g., reaction solutions) to be conveniently added and distributed uniformly.

Abstract translation: 本公开提供了用于微阵列反应装置的覆盖片。 在一个方面，本发明的覆盖片或装置确保了测定样品和测定运行之间的反应单元/体积是稳定和/或一致的，允许样品（例如反应溶液）方便地加入并均匀分布。

公开(公告)号：US20210322980A1

公开(公告)日：2021-10-21

申请号：US17249398

申请日：2021-03-01

Applicant: CAPITALBIO CORPORATION

Inventor： Lei WANG ,  Liang BAI ,  Xiang CHEN ,  Tengfei GUO ,  Xinying ZHOU ,  Jing CHENG ,  Fei WEN ,  Yanan WANG ,  Longtang ZHENG ,  Peng LIU ,  Baolian LI ,  Youchun XU ,  Bin ZHUANG ,  Wanli XING

IPC: B01L3/00

Abstract: A whole-process biological detection device is provided. The whole-process biological detection device belongs to the technical field of biological detection, and includes at least one reactor, where the reactor includes at least one sample-loading unit, at least one liquid release-control unit, at least one extraction unit, at least one liquid switch-control unit, at least one liquid collection unit, at least one liquid transfer unit, at least one liquid flow-control unit, at least one liquid quantitative dispersion unit and multiple reaction cells. The product of the solution has the characteristics of high integration, no need for additional reagents, simple operation, stable and reliable, etc., thereby achieving the goal of sealing the whole process of biological detection and effectively avoiding cross-contamination between samples and equipment.