公开(公告)号：US20230408501A1

公开(公告)日：2023-12-21

申请号：US18241275

申请日：2023-09-01

申请人： The Regents of the University of California

发明人： Ahmet Ali Yanik ,  Xiangchao Zhu

IPC分类号： G01N33/543 ,  B01L3/00 ,  B03C1/01 ,  B03C1/28 ,  B03C1/30 ,  G01N27/48 ,  G01N27/74

CPC分类号： G01N33/54333 ,  B01L3/502761 ,  B03C1/01 ,  B03C1/288 ,  B03C1/30 ,  B03C2201/26 ,  G01N27/745 ,  G01N33/5438 ,  B01L2200/0668 ,  B01L2300/0896 ,  B01L2400/043 ,  G01N27/48

摘要： Methods for detecting submolar concentrations of a target analyte in a sample are disclosed. These methods combine a process of biomarker to bead conversion with bead enrichment and simple visual, optical, or electrochemical detection of the presence of enriched beads to provide sensitive and inexpensive assay for detecting analytes in a sample. Devices for performing these methods are also disclosed.

公开(公告)号：US11747329B2

公开(公告)日：2023-09-05

申请号：US16823592

申请日：2020-03-19

申请人： Western Digital Technologies, Inc.

发明人： Daniel Bedau

IPC分类号： G01N33/543 ,  G01N33/58 ,  G01N33/573 ,  G01N33/542 ,  C12Q1/6869 ,  B01L3/00 ,  G01N27/74

CPC分类号： G01N33/54326 ,  B01L3/502 ,  C12Q1/6869 ,  G01N27/745 ,  G01N33/542 ,  G01N33/54373 ,  G01N33/573 ,  G01N33/585 ,  C12Q1/6869 ,  C12Q2565/607 ,  C12Q2563/143 ,  C12Q2563/155

摘要： Disclosed herein are devices for molecule detection and methods for using detection devices for molecule detection, such as nucleic acid sequencing. In some embodiments, a detection device comprises one or more pole pieces, one or more sensors, each of the one or more sensors coupled to at least one of the one or more pole pieces, and detection circuitry coupled to the one or more sensors. The detection circuitry is configured to detect a characteristic of each of the one or more sensors, the characteristic indicating presence or absence of one or more magnetic nanoparticles (MNPs) coupled to at least one of a plurality of molecules to be detected, and at least one of the one or more pole pieces is operable to draw the one or more MNPs toward at least one of the one or more sensors.

公开(公告)号：US20190120794A1

公开(公告)日：2019-04-25

申请号：US16087833

申请日：2017-03-24

申请人： TDK CORPORATION

发明人： Sachio TSUBOIKE ,  Susumu HARATANI ,  Sumiko KITAGAWA ,  Takashi KIKUKAWA ,  Haruki YUGA

IPC分类号： G01N27/72 ,  G01N33/543 ,  C12Q1/6825

CPC分类号： G01N27/72 ,  C12Q1/6816 ,  C12Q1/6825 ,  G01N27/745 ,  G01N33/54373 ,  C12Q2565/607

摘要： A chemical sensor that enables high detection accuracy that includes a soft-magnetic-material film, a magnetoresistance-effect element, a first film disposed over the soft-magnetic-material film, and a second film disposed over the magnetoresistance-effect element, wherein, when viewed from a direction perpendicular to the film surface of the soft-magnetic-material film, the soft-magnetic-material film is disposed at least partially so as not to overlap the magnetoresistance-effect element; when viewed from the direction perpendicular to the film surface of the soft-magnetic-material film, the magnetoresistance-effect element is disposed at least partially so as not to overlap the soft-magnetic-material film; and the second film has higher solubility in a specific liquid than the first film.

公开(公告)号：US20180303375A1

公开(公告)日：2018-10-25

申请号：US15770364

申请日：2016-10-17

申请人： KONINKLIJKE PHILIPS N.V.

发明人： BERNHARD GLEICH

IPC分类号： A61B5/05 ,  G01N27/74 ,  G01R33/12

CPC分类号： A61B5/0515 ,  A61B2562/0223 ,  G01N27/745 ,  G01R33/1269

摘要： The invention relates to an examination apparatus (100), a method and a computer program for tracking permanently magnetic beads (107) that are transported by a fluid flowing through a channel (106) of an object (105). The examination apparatus (100) comprises at least three magnetic field sensors (101, 102, 103) and an evaluation unit (104). With the magnetic field sensors, the magnetic field caused by the permanently magnetic beads (107) is detected. Due to shear forces acting in the fluid, the permanently magnetic beads (107) are rotating and the magnetic field caused by the beads (107) is temporally varying. This temporal variation of the magnetic field is used by the evaluation unit (104) for discriminating sub-signals related to single beads (107) from the overall signal generated by the magnetic field sensors. Furthermore, the evaluation unit determines positioning information of individual beads on the basis of the discriminated sub-signals.

公开(公告)号：US20180266991A1

公开(公告)日：2018-09-20

申请号：US15459556

申请日：2017-03-15

申请人： QUALCOMM Incorporated

发明人： Jimmy Jianan Kan ,  Peiyuan Wang ,  Chando Park ,  Seung Hyuk Kang

IPC分类号： G01N27/74 ,  G01R33/12 ,  G01R33/09 ,  G01N33/543 ,  G01N27/12

CPC分类号： G01N27/745 ,  G01N27/122 ,  G01N33/5438 ,  G01R33/063 ,  G01R33/09 ,  G01R33/1269 ,  H01F10/3218

摘要： Magneto-impedance (MI) sensors employing current confinement and exchange bias layer(s) for increased MI sensitivity are disclosed. MI sensors may be used as biosensors to detect biological materials. The sensing by the MI devices is based on a giant magneto-impedance (GMI) effect, which is very sensitive to a magnetic field. The GMI effect is a change in impedance of a magnetic material resulting from a change in skin depth of the magnetic material as a function of an external direct current (DC) magnetic field applied to the magnetic material and an alternating current (AC) current flowing through the magnetic material (or adjacent conductive materials). Thus, this change in impedance resulting from a magnetic stray field generated by magnetic nanoparticles can be detected in lower concentrations and measured to determine the amount of magnetic nanoparticles present, and thus the target analyte of interest.

公开(公告)号：US10036729B2

公开(公告)日：2018-07-31

申请号：US14125618

申请日：2012-06-18

申请人： Andrea Ranzoni ,  Menno Willem Jose Prins

发明人： Andrea Ranzoni ,  Menno Willem Jose Prins

IPC分类号： G01N33/553 ,  G01N27/74 ,  G01N21/17 ,  G01N21/51

CPC分类号： G01N27/745 ,  G01N21/1717 ,  G01N21/51 ,  G01N2021/1727 ,  G01N2446/00

摘要： The invention relates to a method and a sensor device (100) for the detection of clusters (C) of magnetic particles (MP) in a sample volume (111), particularly of clusters (C) consisting of two magnetic particles (MP) with different binding sites that are bound to a target molecule in a sandwich configuration. Output light (L2) originating from an interaction of input light (L1) with clusters (C) of magnetic particles (MP) is detected. Moreover, the magnetic particles (MP, C) are actuated by a magnetic actuation field (B), wherein said actuation is at least once interrupted by a pause. In this way a high output signal can be achieved that properly reflects the amount of specifically bound clusters (C).

公开(公告)号：US09927431B2

公开(公告)日：2018-03-27

申请号：US14343252

申请日：2012-09-14

申请人： Jian-Ping Wang ,  Yuanpeng Li ,  Wang Yi

发明人： Jian-Ping Wang ,  Yuanpeng Li ,  Wang Yi

IPC分类号： G01N33/543 ,  G01N27/74 ,  G01R33/09 ,  G01R33/12 ,  G01R33/00 ,  G01R33/07

CPC分类号： G01N33/54326 ,  G01N27/745 ,  G01R33/0052 ,  G01R33/07 ,  G01R33/09 ,  G01R33/093 ,  G01R33/1269 ,  Y10T29/4902

摘要： A biosensor includes a magnetic structure having grooved surface to biologically bond magnetic labels to a biological substance within the grooves. The grooves are positioned within the magnetic structure so that stray magnetic fields from the magnetic structure magnetize magnetic labels within the groove. The magnetic labels may be magnetic nanoparticles or magnetic microbeads. The techniques may reduce or eliminate the usage of any external magnetic field generator, e.g., electromagnets or current lines.

公开(公告)号：US20180038820A1

公开(公告)日：2018-02-08

申请号：US15547722

申请日：2016-01-15

申请人： FUNDACIÓN TEKNIKER

发明人： Santos MERINO ,  Unai ELETXIGERRA ,  Aritz JUAROS ,  Josu MARTÍNEZ

IPC分类号： G01N27/327 ,  B01L3/00 ,  G01N33/58 ,  C12Q1/28 ,  G01N27/49 ,  G01N33/543

CPC分类号： G01N27/3277 ,  B01L3/502761 ,  B01L2200/0631 ,  B01L2200/0668 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/043 ,  B01L2400/0487 ,  C12Q1/28 ,  C12Y111/01007 ,  G01N27/49 ,  G01N27/745 ,  G01N33/54326 ,  G01N33/5438 ,  G01N33/581 ,  G01N2333/908

摘要： A device for detecting and quantifying an analyte in a sample and a method of using the device includes a disposable cartridge having a microfluidic inlet for loading the sample having reporter probe-analyte-capture probe-magnetic bead complexes. A purifying chamber for purifying the magnetic bead complexes is also includes, as well as a measure chamber with a chip having electrodes. External electromagnets are situated each on opposite sides of the purifying chamber for retaining, or releasing or shaking magnetic bead complexes present in the purifying chamber. A permanent magnet is located under the working electrode of the measurement chamber to attract and retain the magnetic bead complexes on the surface of the working electrode of the chip. The device further includes a potentiostat for carrying out the electrochemical detection and quantification of the analyte.

公开(公告)号：US09841421B2

公开(公告)日：2017-12-12

申请号：US13988833

申请日：2011-11-28

申请人： Wendy Uyen Dittmer ,  Mikhail Mikhaylovich Ovsyanko ,  Toon Hendrik Evers ,  Jeroen Hans Nieuwenhuis ,  Joannes Baptist Adrianus Dionisius Van Zon

发明人： Wendy Uyen Dittmer ,  Mikhail Mikhaylovich Ovsyanko ,  Toon Hendrik Evers ,  Jeroen Hans Nieuwenhuis ,  Joannes Baptist Adrianus Dionisius Van Zon

IPC分类号： G01N33/543 ,  G01N27/82 ,  G01N27/72 ,  G01N27/74 ,  G01N35/00 ,  G01N21/27 ,  G01N21/552

CPC分类号： G01N33/54373 ,  G01N21/274 ,  G01N21/552 ,  G01N27/72 ,  G01N27/745 ,  G01N27/825 ,  G01N33/54326 ,  G01N35/0098

摘要： The invention relates to a sensor device (100) and a method for the detection of magnetic particles (1) in a sample chamber (2) with a contact surface (11). The sensor device (100) comprises a sensor unit (120, 130) for detecting magnetic particles (1) in a target region (TR) and/or in at least one reference region on the contact surface. Moreover, it comprises a magnetic field generator (140) for generating a magnetic field that shall guide magnetic particles to the contact surface. With the help of these components, an “auxiliary parameter” is determined that is related to the magnetic particles (1) and/or their movement but that is independent of binding processes taking place in the target region between magnetic particles and the contact surface. The auxiliary parameter may for example be related to the degree of mismatch between the positions reached by the magnetic particles (1) under the influence of a magnetic field and the target region (TR). The evaluation results can be used to validate and/or correct the measurements obtained in the target region (TR).

公开(公告)号：US20170336403A1

公开(公告)日：2017-11-23

申请号：US15520055

申请日：2015-10-16

申请人： Christopher Gordon ATWOOD

发明人： Christopher Gordon ATWOOD

IPC分类号： G01N33/558 ,  G01N21/64 ,  G01N27/74

CPC分类号： G01N33/558 ,  G01N21/6428 ,  G01N21/6445 ,  G01N21/648 ,  G01N27/745 ,  G01N33/542 ,  G01N33/54326 ,  G01N33/582 ,  G01N2021/6439 ,  G01N2021/6463

摘要： In one aspect, presence and/or level of an analyte within a sample is determined by use of a construct comprising a magnetic moiety and a fluorescent moiety. In one embodiment, the construct is magnetically migrated to a transparent surface and then dragged along the surface. In one aspect, an evanescent field is applied and changes in the diffusional or rotational properties of the fluorescent moiety as it migrates in and out of the evanescent field are measured by changes in its fluorescent emission, providing a measure of the interaction between the construct and a component of the sample.