公开(公告)号：US09994687B2

公开(公告)日：2018-06-12

申请号：US14316478

申请日：2014-06-26

申请人： Illumina, Inc.

发明人： Lorenzo Berti ,  Andrew A. Brown ,  Wayne N. George

IPC分类号： C08J7/12 ,  C12Q1/68 ,  C08F8/00 ,  C09D133/26 ,  B01J19/00 ,  C08F220/60

CPC分类号： C08J7/12 ,  B01J19/0046 ,  B01J2219/00529 ,  B01J2219/00533 ,  B01J2219/00619 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00722 ,  C08F8/00 ,  C08F2220/603 ,  C08J2335/00 ,  C09D133/26 ,  C12Q1/6837 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2527/125 ,  C12Q2565/537 ,  C12Q2565/629

摘要： Some embodiments described herein relate to a substrate with a surface comprising a silane or a silane derivative covalently attached to optionally substituted cycloalkene or optionally substituted heterocycloalkene for direct conjugation with a functionalized molecule of interest, such as a polymer, a hydrogel, an amino acid, a nucleoside, a nucleotide, a peptide, a polynucleotide, or a protein. In some embodiments, the silane or silane derivative contains optionally substituted norbornene or norbornene derivatives. Method for preparing a functionalized surface and the use in DNA sequencing and other diagnostic applications are also disclosed.

公开(公告)号：US20180112211A1

公开(公告)日：2018-04-26

申请号：US15834138

申请日：2017-12-07

申请人： Wisconsin Alumni Research Foundation

发明人： Lloyd Smith ,  Cheng-Hsien Wu

IPC分类号： C12N15/10 ,  B01J19/00 ,  C40B50/18

CPC分类号： C12N15/1068 ,  B01J19/0046 ,  B01J2219/005 ,  B01J2219/00529 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00711 ,  B01J2219/00722 ,  C12Q1/6837 ,  C40B50/18 ,  C12Q2525/113

摘要： Described herein are RNA arrays, and compositions and methods for generating RNA arrays, particularly high density RNA arrays. The disclosed methods for generating RNA arrays utilize template DNA arrays and RNA polymerase to generate RNA arrays. In some embodiments, the disclosed methods use an RNA polymerase and modified ribonucleosides to generate modified RNA arrays for various applications, e.g. RNA arrays having higher nuclease resistance, more conformationally stable RNA arrays, and higher binding affinity RNA aptamer arrays. In some embodiments, the disclosed methods are used to generate RNA bead arrays.

公开(公告)号：US09719929B2

公开(公告)日：2017-08-01

申请号：US13981787

申请日：2011-12-26

申请人： Kumie Ozaki ,  Hiromichi Sasamoto ,  Kunihisa Nagino

发明人： Kumie Ozaki ,  Hiromichi Sasamoto ,  Kunihisa Nagino

IPC分类号： G01N33/48 ,  G01N33/50 ,  G01N21/64 ,  G01N33/543 ,  G01N33/58 ,  G06F19/20 ,  C40B60/12

CPC分类号： G01N21/6486 ,  B01J2219/00529 ,  B01J2219/00576 ,  B01J2219/00693 ,  C40B60/12 ,  G01N21/6452 ,  G01N21/6456 ,  G01N33/54373 ,  G01N33/582 ,  G06F19/20

摘要： A microarray analysis method, in which a microarray obtained by arranging probes on a substrate surface having an irregular shape is irradiated with excitation light and fluorescence amounts of the probes excited by the excitation light are obtained as numerical data, includes a step (a) of measuring the fluorescence amounts of the probes to acquire fluorescence image data, a step (b) of receiving reflected light and/or scattered light from the substrate surface to acquire the irregular shape of the substrate surface of the microarray as alignment image data based on the light receiving intensities of the light, and a step (c) of determining positions of the probes on the fluorescence image data based on the alignment image data.

公开(公告)号：US09669376B2

公开(公告)日：2017-06-06

申请号：US14656267

申请日：2015-03-12

申请人： Agena Bioscience, Inc.

发明人： Thomas Becker ,  Aaron A. Hanson

IPC分类号： B01J19/00 ,  B01L3/02 ,  B82Y30/00 ,  G01N35/10 ,  G01N1/10 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N35/00 ,  G01N35/02

CPC分类号： B01J19/0046 ,  B01J2219/00387 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00686 ,  B01J2219/00689 ,  B01J2219/00691 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L3/0244 ,  B01L3/0248 ,  B01L2200/0657 ,  B82Y30/00 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N1/10 ,  G01N35/0099 ,  G01N35/028 ,  G01N35/10 ,  G01N35/1016 ,  G01N35/1065 ,  G01N2035/00158 ,  G01N2035/1037 ,  Y10T436/11 ,  Y10T436/112499 ,  Y10T436/119163 ,  Y10T436/24 ,  Y10T436/2575

摘要： A substrate for use in mass spectrometric analyzes having an array of target locations.

公开(公告)号：US20170147748A1

公开(公告)日：2017-05-25

申请号：US15426860

申请日：2017-02-07

申请人： Synthetic Genomics, Inc.

发明人： Peer F. Staehler ,  Cord F. Staehler ,  Manfred Mueller

IPC分类号： G06F19/22 ,  G06F19/28 ,  C12P19/34

CPC分类号： G16B30/00 ,  B01J19/0046 ,  B01J19/0093 ,  B01J2219/00317 ,  B01J2219/00432 ,  B01J2219/00436 ,  B01J2219/00439 ,  B01J2219/00441 ,  B01J2219/00448 ,  B01J2219/00479 ,  B01J2219/00497 ,  B01J2219/00511 ,  B01J2219/00529 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00603 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00675 ,  B01J2219/00689 ,  B01J2219/00702 ,  B01J2219/00704 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L3/502707 ,  B01L3/5085 ,  B01L2300/0654 ,  B01L2300/069 ,  B01L2300/0864 ,  B82Y30/00 ,  C12N15/10 ,  C12N15/66 ,  C12P19/34 ,  C40B40/06 ,  C40B40/10 ,  C40B50/14 ,  G03F7/70216 ,  G16B50/00

摘要： The invention relates to a method for producing polymers, in particular synthetic nucleic acid double strands of optional sequence, comprising the steps: (a) provision of a support having a surface area which contains a plurality of individual reaction areas, (b) location-resolved synthesis of nucleic acid fragments having in each case different base sequences in several of the individual reaction areas, and (c) detachment of the nucleic acid fragments from individual reaction areas.

公开(公告)号：US20160256846A1

公开(公告)日：2016-09-08

申请号：US15162304

申请日：2016-05-23

申请人： ILLUMINA CAMBRIDGE LIMITED

发明人： Mark Edward Brennan Smith ,  Andrea Sabot ,  Isabelle Marie Julia Rasolonjatovo ,  Jean-Ernest Sohna Sohna ,  Adrian Martin Horgan ,  Harold Philip Swerdlow

IPC分类号： B01J19/00 ,  C08F222/38 ,  C12Q1/68

CPC分类号： B01J19/0046 ,  B01J2219/00351 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00639 ,  B01J2219/00641 ,  B01J2219/00716 ,  B01J2219/0072 ,  B01J2219/00722 ,  C08F222/38 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6837 ,  C12Q1/6876 ,  C40B40/06 ,  C40B50/18 ,  Y10T428/265

摘要： Methods and compositions are disclosed relating to the localization of nucleic acids to arrays such as silane-free arrays, and of sequencing the nucleic acids localized thereby.

摘要翻译： 公开了关于将核酸定位到诸如无硅烷基阵列的阵列的方法和组合物，以及对其定位的核酸进行测序。

公开(公告)号：US09416418B2

公开(公告)日：2016-08-16

申请号：US14513788

申请日：2014-10-14

申请人： Seiko Epson Corporation

发明人： Fumio Takagi

IPC分类号： C12P19/34 ,  C12Q1/68 ,  B01L3/00 ,  B01L7/00 ,  G06F19/18

CPC分类号： C12Q1/6876 ,  B01J2219/00317 ,  B01J2219/00421 ,  B01J2219/00529 ,  B01J2219/00576 ,  B01J2219/00608 ,  B01J2219/00693 ,  B01J2219/00702 ,  B01J2219/00722 ,  B01L3/5025 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/50851 ,  B01L7/52 ,  B01L2200/0673 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/161 ,  B01L2400/0409 ,  C12Q1/6851 ,  G06F19/18 ,  C12Q2563/107

摘要： A biochip used for quantitative analysis of a target DNA contained in a sample. The biochip includes a type I chamber that includes a primer designed to bind to the target DNA, an internal standard DNA of a first amount that has a sequence different from a sequence of the target DNA, and is amplifiable with the primer, and a fluorescent probe that is designed to bind to a part of a PCR product of the target DNA and to a part of a PCR product of the internal standard DNA. The fluorescent probe fluoresces differently for the PCR product of the target DNA and the PCR product of the internal standard DNA. The biochip also includes a type II chamber that includes the internal standard DNA of a second amount, the primer, and the fluorescent probe. The first and second amounts are different.

公开(公告)号：US09334530B2

公开(公告)日：2016-05-10

申请号：US12779493

申请日：2010-05-13

申请人： Mark J. Lim ,  Kenneth J. Rothschild

发明人： Mark J. Lim ,  Kenneth J. Rothschild

IPC分类号： G01N33/53 ,  C12Q1/68 ,  B01J19/00 ,  B82Y30/00

CPC分类号： C12Q1/6837 ,  B01J19/0046 ,  B01J2219/005 ,  B01J2219/00529 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00722 ,  B82Y30/00 ,  C12Q1/6844 ,  C12Q2565/537 ,  C12Q2563/155

摘要： Methods of amplifying nucleic acid on a solid support are described. Beads and template, each in known concentrations, are employed so a range of template to bead ratios can be exploited. Where the beads contain primers, the template can be amplified. After amplification, non-covalently bound template is removed, so as to leave beads with extended primers (or beads with primers that were not extended).

摘要翻译： 描述了在固体支持物上扩增核酸的方法。 使用已知浓度的珠和模板，因此可以利用范围的模板与珠比率。 当珠含有引物时，可以扩增模板。 扩增后，除去非共价结合的模板，以便用延长的引物（或具有未延伸的引物的珠）留下珠。

公开(公告)号：US09206477B2

公开(公告)日：2015-12-08

申请号：US14513906

申请日：2014-10-14

申请人： Cornell Research Foundation, Inc.

发明人： Francis Barany ,  George Barany ,  Robert P. Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

IPC分类号： C07H21/04 ,  C12N15/11 ,  C12Q1/68 ,  B01J19/00 ,  B82Y30/00 ,  C40B40/06 ,  C40B60/14

CPC分类号： C12Q1/6827 ,  B01J19/0046 ,  B01J2219/00432 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00536 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00729 ,  B82Y30/00 ,  C12Q1/6816 ,  C12Q1/6837 ,  C12Q1/6876 ,  C12Q2600/156 ,  C40B40/06 ,  C40B60/14 ,  Y02A50/58 ,  C12Q2565/501 ,  C12Q2561/125 ,  C12Q2537/143 ,  C12Q2565/514

摘要： The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support.

摘要翻译： 本发明描述了用于鉴定多个靶核苷酸序列中一个或多个单碱基变化，插入，缺失或易位不同的多个序列中的一个或多个的方法。 该方法包括连接阶段，捕获阶段和检测阶段。 连接阶段利用具有目标序列特异性部分的一个寡核苷酸探针与可寻址阵列特异性部分之间的连接检测反应和具有靶序列特异性部分和可检测标记的第二寡核苷酸探针。 在连接阶段之后，捕获阶段通过将连接的寡核苷酸探针与具有固定化捕获寡核苷酸阵列的固体支持物杂交进行，其中至少一些与可寻址阵列特异性部分互补。 捕获阶段完成后，进行检测阶段以检测与固体支持物杂交的连接的寡核苷酸探针的标记。

公开(公告)号：US20150308957A1

公开(公告)日：2015-10-29

申请号：US14793678

申请日：2015-07-07

申请人： Emergence Genomics, LLC

发明人： Michael Okura ,  Bruce J. Richardson

IPC分类号： G01N21/64 ,  B01L7/00 ,  G01N21/03

CPC分类号： G01N21/6408 ,  B01J2219/00529 ,  B01J2219/00722 ,  B01L7/00 ,  B01L2300/0636 ,  B01L2300/0822 ,  B01L2300/088 ,  B01L2300/1822 ,  B01L2300/1838 ,  B01L2400/0487 ,  C12Q1/68 ,  G01N21/0332 ,  G01N21/6428 ,  G01N21/6452 ,  G01N2021/6441

摘要： A method and apparatus for performing melting curve analyses of nucleic acids on a microarray is described. The present method includes varying the temperature of a fluid on a microarray to dissociate and remove target DNA, scanning the mircoarray for fluorescence, collecting the target DNA removed from the microarray, and reusing the collected target DNA and the microarray. The apparatus of the present disclosure includes a microarray stage, a light source and detector, and a temperature controller, wherein the temperature controller is configured to adjust the temperature of a fluid within a sample chamber on the microarray such that the temperature of the fluid is varied during the analysis such that target DNA is dissociated from the microarray, and wherein the light source is directed to the microarray and the resulting fluorescence is perceived by the detector.

摘要翻译： 描述了在微阵列上进行核酸的熔解曲线分析的方法和装置。 本发明的方法包括改变微阵列上的流体的温度以解离和去除目标DNA，扫描微阵列进行荧光，收集从微阵列移除的靶DNA，并重新使用收集的靶DNA和微阵列。 本公开的装置包括微阵列级，光源和检测器以及温度控制器，其中温度控制器被配置为调节微阵列上的样品室内的流体的温度，使得流体的温度为 在分析期间变化，使得靶DNA从微阵列解离，并且其中光源被引导到微阵列，并且所得到的荧光被检测器感知。