公开(公告)号：US20150308957A1

公开(公告)日：2015-10-29

申请号：US14793678

申请日：2015-07-07

申请人： Emergence Genomics, LLC

发明人： Michael Okura ,  Bruce J. Richardson

IPC分类号： G01N21/64 ,  B01L7/00 ,  G01N21/03

CPC分类号： G01N21/6408 ,  B01J2219/00529 ,  B01J2219/00722 ,  B01L7/00 ,  B01L2300/0636 ,  B01L2300/0822 ,  B01L2300/088 ,  B01L2300/1822 ,  B01L2300/1838 ,  B01L2400/0487 ,  C12Q1/68 ,  G01N21/0332 ,  G01N21/6428 ,  G01N21/6452 ,  G01N2021/6441

摘要： A method and apparatus for performing melting curve analyses of nucleic acids on a microarray is described. The present method includes varying the temperature of a fluid on a microarray to dissociate and remove target DNA, scanning the mircoarray for fluorescence, collecting the target DNA removed from the microarray, and reusing the collected target DNA and the microarray. The apparatus of the present disclosure includes a microarray stage, a light source and detector, and a temperature controller, wherein the temperature controller is configured to adjust the temperature of a fluid within a sample chamber on the microarray such that the temperature of the fluid is varied during the analysis such that target DNA is dissociated from the microarray, and wherein the light source is directed to the microarray and the resulting fluorescence is perceived by the detector.

摘要翻译： 描述了在微阵列上进行核酸的熔解曲线分析的方法和装置。 本发明的方法包括改变微阵列上的流体的温度以解离和去除目标DNA，扫描微阵列进行荧光，收集从微阵列移除的靶DNA，并重新使用收集的靶DNA和微阵列。 本公开的装置包括微阵列级，光源和检测器以及温度控制器，其中温度控制器被配置为调节微阵列上的样品室内的流体的温度，使得流体的温度为 在分析期间变化，使得靶DNA从微阵列解离，并且其中光源被引导到微阵列，并且所得到的荧光被检测器感知。