公开(公告)号：US20240327821A1

公开(公告)日：2024-10-03

申请号：US18442492

申请日：2024-02-15

申请人： Athebio AG

发明人： Patrik Forrer ,  Rohan Sakariah Eapen

IPC分类号： C12N15/10 ,  C07K14/00 ,  C40B40/10

CPC分类号： C12N15/1055 ,  C07K14/001 ,  C12N15/1089 ,  C40B40/10

摘要： Described herein are proteins comprising an ankyrin repeat domain with a mutation in the internal ankyrin repeat that is adjacent to the N-terminal capping module, in particular a mutation at position 23 of said internal ankyrin repeat, as well as related products and methods.

公开(公告)号：US12091777B2

公开(公告)日：2024-09-17

申请号：US17030216

申请日：2020-09-23

申请人： Twist Bioscience Corporation

发明人： Ray Tabibiazar ,  Aaron Sato ,  Pankaj Garg ,  Qiang Liu

IPC分类号： C40B40/10 ,  C07K16/28 ,  C12N15/10 ,  C40B40/08

CPC分类号： C40B40/10 ,  C07K16/2896 ,  C12N15/1044 ,  C40B40/08 ,  C07K2317/24 ,  C07K2317/565 ,  C07K2317/92

摘要： Provided herein are methods and compositions relating to prostaglandin D2 receptor 2 (DP2 or CRTH2R) libraries having nucleic acids encoding for a scaffold comprising a CRTH2R binding domain. CRTH2R libraries described herein encode for immunoglobulins including antibodies and single domain antibodies. Libraries described herein include variegated libraries comprising nucleic acids each encoding for a predetermined variant of at least one predetermined reference nucleic acid sequence. Further described herein are protein libraries generated when the nucleic acid libraries are translated. Further described herein are cell libraries expressing variegated nucleic acid libraries described herein.

公开(公告)号：US12071710B2

公开(公告)日：2024-08-27

申请号：US17058124

申请日：2019-05-31

申请人： MOGAM INSTITUTE FOR BIOMEDICAL RESEARCH

发明人： Dong-Sik Kim ,  Mi Jung Lee ,  Mi Young Oh ,  Hye-Ji Choi ,  Gil-Jung Kim ,  Shin A Jang ,  Ae Rin Yoon

IPC分类号： C40B40/10 ,  C07K16/00 ,  C07K16/28 ,  C12N15/10

CPC分类号： C40B40/10 ,  C07K16/005 ,  C07K16/28 ,  C12N15/1037 ,  C07K2317/40 ,  C07K2317/55 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/94

摘要： The present invention relates to a novel antibody library and an antibody-screening method using same. Having a human sequence-derived specific VH or VL scaffold, the antibody library according to the present invention exhibits high thermodynamic stability and enjoys the advantages of allowing high soluble expression as well as reversible folding. In addition, the antibody according to the present invention includes a variety of rationally controlled CDRs so as to exhibit high specificity and high affinity to all antigens and thus can be advantageously used for selecting an adequate candidate antibody against a target antigen.

公开(公告)号：US20240271329A1

公开(公告)日：2024-08-15

申请号：US18281854

申请日：2022-02-18

申请人： Yafei Shanghai Biology Medicine Science & Technology Co. Ltd.

发明人： Wei WEI ,  Chen LIU ,  Bin CHEN ,  Yuan LIU, ,  Yuanchao BAI

IPC分类号： C40B50/18 ,  C40B40/10 ,  C40B50/06 ,  C40B70/00 ,  C40B80/00

CPC分类号： C40B50/18 ,  C40B40/10 ,  C40B50/06 ,  C40B70/00 ,  C40B80/00

摘要： The invention relates to a cyclic compound library and a method for making cyclic compounds. The method includes reacting a solid-phase carrier, a molecule containing a photocleavable group, a linking molecule, a building block, and an A-end ring-closure molecule and a B-end ring-closure molecule at the two ends for synthesizing. The method also includes removing the solid-phase carrier using decomposition under light radiation and performing a ring-closure reaction using amino acid residue structures of the A-end ring-closure molecule A and the B-end ring-closure molecule B under the action of a cyclic peptide synthetase.

公开(公告)号：US12060655B2

公开(公告)日：2024-08-13

申请号：US17316381

申请日：2021-05-10

申请人： NantBio, Inc.

发明人： Clifford Anders Olson ,  Kayvan Niazi

IPC分类号： C40B50/06 ,  C12N5/09 ,  C12N15/10 ,  C12N15/86 ,  C40B40/02 ,  C40B40/10 ,  C12N15/85

CPC分类号： C40B50/06 ,  C12N5/0693 ,  C12N15/1037 ,  C12N15/1062 ,  C12N15/86 ,  C40B40/02 ,  C40B40/10 ,  C07K2317/55 ,  C07K2317/565 ,  C12N2015/8518

摘要： Compositions, methods and uses of a recombinant virus and/or recombinant viral vector encoding a distinct antibody or antibody fragment generated from high-diversity nucleic acid library are presented. Preferably, the recombinant virus is genetically modified, low immunogenic virus, for example, an E2b-deleted adenovirus. The high-diversity nucleic acid library comprises or is derived from (1) a VH-CDR1/2 sub-library, (2) a plurality of VH-CDR3 sub-libraries, and (3) a VL sub-library, each of which comprises a plurality of members. Preferably, each member of the sub-libraries comprises at least one random cassette that has a plurality of degenerate base positions. In an especially preferred embodiment, at least portions of at least two members of the VH-CDR1/2 sub-library, the plurality of VH-CDR3 sub-libraries, and the VL sub-library are recombined to form an expression library member in an expression library, where each member of the expression library encodes a distinct antibody or antibody fragment.

公开(公告)号：US20240263355A1

公开(公告)日：2024-08-08

申请号：US18622742

申请日：2024-03-29

申请人： NantBio, Inc.

发明人： Clifford Anders Olson ,  Kayvan Niazi

IPC分类号： C40B50/06 ,  C12N5/09 ,  C12N15/10 ,  C12N15/85 ,  C12N15/86 ,  C40B40/02 ,  C40B40/10

CPC分类号： C40B50/06 ,  C12N5/0693 ,  C12N15/1037 ,  C12N15/1062 ,  C12N15/86 ,  C40B40/02 ,  C40B40/10 ,  C07K2317/55 ,  C07K2317/565 ,  C12N2015/8518

摘要： Compositions, methods and uses of a recombinant virus and/or recombinant viral vector encoding a distinct antibody or antibody fragment generated from high-diversity nucleic acid library are presented. Preferably, the recombinant virus is genetically modified, low immunogenic virus, for example, an E2b-deleted adenovirus. The high-diversity nucleic acid library comprises or is derived from (1) a VH-CDR1/2 sub-library, (2) a plurality of VH-CDR3 sub-libraries, and (3) a VL sub-library, each of which comprises a plurality of members. Preferably, each member of the sub-libraries comprises at least one random cassette that has a plurality of degenerate base positions. In an especially preferred embodiment, at least portions of at least two members of the VH-CDR1/2 sub-library, the plurality of VH-CDR3 sub-libraries, and the VL sub-library are recombined to form an expression library member in an expression library, where each member of the expression library encodes a distinct antibody or antibody fragment.

公开(公告)号：US20240132875A1

公开(公告)日：2024-04-25

申请号：US18133377

申请日：2023-04-11

申请人： The Trustees of the California State University

发明人： Kambiz Hamadani

IPC分类号： C12N15/10 ,  C07K1/13 ,  C12Q1/6874 ,  C40B40/08 ,  C40B40/10

CPC分类号： C12N15/1062 ,  C07K1/13 ,  C12N15/102 ,  C12N15/1065 ,  C12Q1/6874 ,  C40B40/08 ,  C40B40/10

摘要： Aspects of the present disclosure include methods of producing modified polypeptides and modified polypeptide-ribosome or polypeptide-mRNA complexes, and methods of screening polynucleotide and polypeptide libraries. The present disclosure also provides polypeptide libraries useful in screening for single molecule phenotypes. Also provided are kits useful for producing polypeptides capable of being modified using methods disclosed herein.

公开(公告)号：US20240118273A1

公开(公告)日：2024-04-11

申请号：US18368931

申请日：2023-09-15

申请人： Vibrant Holdings, LLC

发明人： John J. Rajasekaran ,  Vasanth Jayaraman ,  Tianhao Wang ,  Kang Bei ,  Hari Krishnan Krishnamurthy

IPC分类号： G01N33/543 ,  C40B40/06 ,  C40B40/10 ,  G01N35/00 ,  G16B20/00

CPC分类号： G01N33/54366 ,  C40B40/06 ,  C40B40/10 ,  G01N33/54386 ,  G01N35/00029 ,  G01N35/00069 ,  G01N35/0099 ,  G16B20/00 ,  G01N2035/00158 ,  G01N2035/00168 ,  G01N2035/1034

摘要： Disclosed herein are formulations, substrates, and arrays. Also disclosed herein are methods for manufacturing and methods of assuring a uniformly high quality of a microarray of features that are attached to a surface of the microarray at positionally-defined locations.

公开(公告)号：US20240084287A1

公开(公告)日：2024-03-14

申请号：US18327345

申请日：2023-06-01

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology

发明人： Paul Blainey ,  Navpreet Ranu ,  Todd Gierahn ,  J. Christopher Love

IPC分类号： C12N15/10 ,  C12N1/20 ,  C12Q1/6806 ,  C12Q1/6869 ,  C40B30/04 ,  C40B40/06 ,  C40B40/10 ,  C40B50/06

CPC分类号： C12N15/1006 ,  C12N1/205 ,  C12Q1/6806 ,  C12Q1/6869 ,  C40B30/04 ,  C40B40/06 ,  C40B40/10 ,  C40B50/06 ,  C12N2310/20 ,  C12R2001/465 ,  C12Y301/11 ,  C12Y302/02027

摘要： The present invention relates to the analysis of complex single cell sequencing libraries. Disclosed are methods for enrichment of library members based on the presence of cell-of origin barcodes to identify and concentrate DNA that is relevant to interesting cells or components that would be expensive or difficult to study otherwise. Also, disclosed are methods of capturing cDNA library molecules by use of CRISPR systems, hybridization or PCR. The present invention allows for identifying the properties of rare cells in single cell RNA-seq data and accurately profile them through clustering approaches. Further information on transcript abundances from subpopulations of single cells can be analyzed at a lower sequencing effort. The methods also allow for linking TCR alpha and beta chains at the single cell level.

公开(公告)号：US11866484B2

公开(公告)日：2024-01-09

申请号：US17190626

申请日：2021-03-03

申请人： INSERM (Institut National de la Sante et de la Recherche Medicale) ,  Institut Curie ,  Universite Paul Sabatier Toulouse III ,  Centre National de la Recherche Scientifique (CNRS)

发明人： Aurelien Olichon ,  Sandrine Moutel ,  Franck Perez

IPC分类号： C40B50/06 ,  C07K16/00 ,  C07K16/32 ,  C07K16/44 ,  C40B40/08 ,  C07K16/40 ,  C40B40/10

CPC分类号： C07K16/005 ,  C07K16/32 ,  C07K16/40 ,  C07K16/44 ,  C40B40/08 ,  C07K2317/22 ,  C07K2317/24 ,  C07K2317/565 ,  C07K2317/569 ,  C07K2317/76 ,  C07K2317/80 ,  C07K2317/92 ,  C40B40/10 ,  C40B50/06

摘要： The invention relates to the identification of a highly stable single domain antibody scaffold (hs2dAb) and its use in generating synthetic single domain antibody library (hs2dAb-L1). The invention also relates to antigen-binding proteins comprising said stable single domain antibody scaffold and their uses, in particular as therapeutics.