公开(公告)号：US12196749B2

公开(公告)日：2025-01-14

申请号：US16942130

申请日：2020-07-29

Applicant: Meso Scale Technologies, LLC.

Inventor： Eli N. Glezer ,  Jonathan K. Leland ,  Mark A. Billadeau ,  Joseph M. Leginus ,  Bandele Jeffrey-Coker ,  Jeff D. Debad ,  Koustubh A. Phalnikar ,  Sriram Jambunathan

IPC: G01N33/543 ,  B01J19/00 ,  B01L3/00 ,  B01L9/00 ,  C12Q1/00 ,  G01N21/76 ,  G01N27/30 ,  B33Y80/00 ,  C40B40/10

Abstract: Assay modules, preferably assay cartridges, are described as are reader apparatuses which may be used to control aspects of module operation. The modules preferably comprise a detection chamber with integrated electrodes that may be used for carrying out electrode induced luminescence measurements. Methods are described for immobilizing assay reagents in a controlled fashion on these electrodes and other surfaces. Assay modules and cartridges are also described that have a detection chamber, preferably having integrated electrodes, and other fluidic components which may include sample chambers, waste chambers, conduits, vents, bubble traps, reagent chambers, dry reagent pill zones and the like. In certain preferred embodiments, these modules are adapted to receive and analyze a sample collected on an applicator stick.

公开(公告)号：US12187813B2

公开(公告)日：2025-01-07

申请号：US18167350

申请日：2023-02-10

Applicant: Florida Atlantic University Board of Trustees ,  University of Florida Research Foundation

Inventor： Predrag Cudic ,  Jay McLaughlin

IPC: A61K31/4045 ,  A61K9/00 ,  A61K47/64 ,  A61K47/65 ,  A61P25/04 ,  A61P25/06 ,  A61P25/16 ,  A61P25/18 ,  A61P25/28 ,  A61P25/36 ,  C07K5/12 ,  C07K7/64 ,  A61K38/00 ,  C40B40/10

Abstract: Novel cyclic peptides, cyclic peptide conjugates and compositions containing them for treating neurological diseases in a subject include an Odorranalectin (OL) sequence or modified OL sequence as a scaffold and a biologically active peptide or protein and/or therapeutic agent conjugated thereto. Methods of treatment of neurological diseases are based on intranasal delivery of a cyclic peptide or cyclic peptide conjugate as described herein. Combinatorial libraries that include a plurality of cyclic peptides have also been developed and can be used to screen for a ligand(s) for a receptor of interest.

公开(公告)号：US20240384437A1

公开(公告)日：2024-11-21

申请号：US18430601

申请日：2024-02-01

Applicant: RULES-BASED MEDICINE INC.

Inventor： Andrew Raymon Morton BRADBURY ,  Michael Frank ERASMUS ,  Andre TEIXEIRA

IPC: C40B40/10 ,  C12N15/10 ,  C12N15/81 ,  C40B40/08 ,  C40B50/06

Abstract: Antibody libraries comprising a plurality of heavy chain variable domains and/or a plurality of light chain variable domains, which comprise complementary determining regions (CDRs) found in naturally-occurring human antibodies, and methods of making such antibody libraries. The antibody libraries are free of members that comprise one or more liabilities affecting one or more features of such members. Further, the antibody libraries comprise members having heavy chain and/or light chain CDRs not found in the same naturally-occurring human antibody.

公开(公告)号：US20240327821A1

公开(公告)日：2024-10-03

申请号：US18442492

申请日：2024-02-15

Applicant: Athebio AG

Inventor： Patrik Forrer ,  Rohan Sakariah Eapen

IPC: C12N15/10 ,  C07K14/00 ,  C40B40/10

CPC classification number: C12N15/1055 ,  C07K14/001 ,  C12N15/1089 ,  C40B40/10

Abstract: Described herein are proteins comprising an ankyrin repeat domain with a mutation in the internal ankyrin repeat that is adjacent to the N-terminal capping module, in particular a mutation at position 23 of said internal ankyrin repeat, as well as related products and methods.

公开(公告)号：US12091777B2

公开(公告)日：2024-09-17

申请号：US17030216

申请日：2020-09-23

Applicant: Twist Bioscience Corporation

Inventor： Ray Tabibiazar ,  Aaron Sato ,  Pankaj Garg ,  Qiang Liu

IPC: C40B40/10 ,  C07K16/28 ,  C12N15/10 ,  C40B40/08

CPC classification number: C40B40/10 ,  C07K16/2896 ,  C12N15/1044 ,  C40B40/08 ,  C07K2317/24 ,  C07K2317/565 ,  C07K2317/92

Abstract: Provided herein are methods and compositions relating to prostaglandin D2 receptor 2 (DP2 or CRTH2R) libraries having nucleic acids encoding for a scaffold comprising a CRTH2R binding domain. CRTH2R libraries described herein encode for immunoglobulins including antibodies and single domain antibodies. Libraries described herein include variegated libraries comprising nucleic acids each encoding for a predetermined variant of at least one predetermined reference nucleic acid sequence. Further described herein are protein libraries generated when the nucleic acid libraries are translated. Further described herein are cell libraries expressing variegated nucleic acid libraries described herein.

公开(公告)号：US12071710B2

公开(公告)日：2024-08-27

申请号：US17058124

申请日：2019-05-31

Applicant: MOGAM INSTITUTE FOR BIOMEDICAL RESEARCH

Inventor： Dong-Sik Kim ,  Mi Jung Lee ,  Mi Young Oh ,  Hye-Ji Choi ,  Gil-Jung Kim ,  Shin A Jang ,  Ae Rin Yoon

IPC: C40B40/10 ,  C07K16/00 ,  C07K16/28 ,  C12N15/10

CPC classification number: C40B40/10 ,  C07K16/005 ,  C07K16/28 ,  C12N15/1037 ,  C07K2317/40 ,  C07K2317/55 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/94

Abstract: The present invention relates to a novel antibody library and an antibody-screening method using same. Having a human sequence-derived specific VH or VL scaffold, the antibody library according to the present invention exhibits high thermodynamic stability and enjoys the advantages of allowing high soluble expression as well as reversible folding. In addition, the antibody according to the present invention includes a variety of rationally controlled CDRs so as to exhibit high specificity and high affinity to all antigens and thus can be advantageously used for selecting an adequate candidate antibody against a target antigen.

公开(公告)号：US20240271329A1

公开(公告)日：2024-08-15

申请号：US18281854

申请日：2022-02-18

Applicant: Yafei Shanghai Biology Medicine Science & Technology Co. Ltd.

Inventor： Wei WEI ,  Chen LIU ,  Bin CHEN ,  Yuan LIU, ,  Yuanchao BAI

IPC: C40B50/18 ,  C40B40/10 ,  C40B50/06 ,  C40B70/00 ,  C40B80/00

CPC classification number: C40B50/18 ,  C40B40/10 ,  C40B50/06 ,  C40B70/00 ,  C40B80/00

Abstract: The invention relates to a cyclic compound library and a method for making cyclic compounds. The method includes reacting a solid-phase carrier, a molecule containing a photocleavable group, a linking molecule, a building block, and an A-end ring-closure molecule and a B-end ring-closure molecule at the two ends for synthesizing. The method also includes removing the solid-phase carrier using decomposition under light radiation and performing a ring-closure reaction using amino acid residue structures of the A-end ring-closure molecule A and the B-end ring-closure molecule B under the action of a cyclic peptide synthetase.

公开(公告)号：US12060655B2

公开(公告)日：2024-08-13

申请号：US17316381

申请日：2021-05-10

Applicant: NantBio, Inc.

Inventor： Clifford Anders Olson ,  Kayvan Niazi

IPC: C40B50/06 ,  C12N5/09 ,  C12N15/10 ,  C12N15/86 ,  C40B40/02 ,  C40B40/10 ,  C12N15/85

CPC classification number: C40B50/06 ,  C12N5/0693 ,  C12N15/1037 ,  C12N15/1062 ,  C12N15/86 ,  C40B40/02 ,  C40B40/10 ,  C07K2317/55 ,  C07K2317/565 ,  C12N2015/8518

Abstract: Compositions, methods and uses of a recombinant virus and/or recombinant viral vector encoding a distinct antibody or antibody fragment generated from high-diversity nucleic acid library are presented. Preferably, the recombinant virus is genetically modified, low immunogenic virus, for example, an E2b-deleted adenovirus. The high-diversity nucleic acid library comprises or is derived from (1) a VH-CDR1/2 sub-library, (2) a plurality of VH-CDR3 sub-libraries, and (3) a VL sub-library, each of which comprises a plurality of members. Preferably, each member of the sub-libraries comprises at least one random cassette that has a plurality of degenerate base positions. In an especially preferred embodiment, at least portions of at least two members of the VH-CDR1/2 sub-library, the plurality of VH-CDR3 sub-libraries, and the VL sub-library are recombined to form an expression library member in an expression library, where each member of the expression library encodes a distinct antibody or antibody fragment.

公开(公告)号：US20240263355A1

公开(公告)日：2024-08-08

申请号：US18622742

申请日：2024-03-29

Applicant: NantBio, Inc.

Inventor： Clifford Anders Olson ,  Kayvan Niazi

IPC: C40B50/06 ,  C12N5/09 ,  C12N15/10 ,  C12N15/85 ,  C12N15/86 ,  C40B40/02 ,  C40B40/10

CPC classification number: C40B50/06 ,  C12N5/0693 ,  C12N15/1037 ,  C12N15/1062 ,  C12N15/86 ,  C40B40/02 ,  C40B40/10 ,  C07K2317/55 ,  C07K2317/565 ,  C12N2015/8518

Abstract: Compositions, methods and uses of a recombinant virus and/or recombinant viral vector encoding a distinct antibody or antibody fragment generated from high-diversity nucleic acid library are presented. Preferably, the recombinant virus is genetically modified, low immunogenic virus, for example, an E2b-deleted adenovirus. The high-diversity nucleic acid library comprises or is derived from (1) a VH-CDR1/2 sub-library, (2) a plurality of VH-CDR3 sub-libraries, and (3) a VL sub-library, each of which comprises a plurality of members. Preferably, each member of the sub-libraries comprises at least one random cassette that has a plurality of degenerate base positions. In an especially preferred embodiment, at least portions of at least two members of the VH-CDR1/2 sub-library, the plurality of VH-CDR3 sub-libraries, and the VL sub-library are recombined to form an expression library member in an expression library, where each member of the expression library encodes a distinct antibody or antibody fragment.

公开(公告)号：US20240132875A1

公开(公告)日：2024-04-25

申请号：US18133377

申请日：2023-04-11

Applicant: The Trustees of the California State University

Inventor： Kambiz Hamadani

IPC: C12N15/10 ,  C07K1/13 ,  C12Q1/6874 ,  C40B40/08 ,  C40B40/10

CPC classification number: C12N15/1062 ,  C07K1/13 ,  C12N15/102 ,  C12N15/1065 ,  C12Q1/6874 ,  C40B40/08 ,  C40B40/10

Abstract: Aspects of the present disclosure include methods of producing modified polypeptides and modified polypeptide-ribosome or polypeptide-mRNA complexes, and methods of screening polynucleotide and polypeptide libraries. The present disclosure also provides polypeptide libraries useful in screening for single molecule phenotypes. Also provided are kits useful for producing polypeptides capable of being modified using methods disclosed herein.