公开(公告)号：US12173282B2

公开(公告)日：2024-12-24

申请号：US17030232

申请日：2020-09-23

Applicant: Twist Bioscience Corporation

Inventor： Aaron Sato ,  Pankaj Garg ,  Qiang Liu ,  Tom Yuan

IPC: C12N15/10 ,  C07K16/28

Abstract: Provided herein are methods and compositions relating to variant nucleic acid libraries encoding for antibodies including single domain antibodies. Libraries generated using methods described herein have improved characteristics including improved binding affinity. Libraries described herein include variegated libraries comprising nucleic acids each encoding for a predetermined variant of at least one predetermined reference nucleic acid sequence. Further described herein are protein libraries generated when the nucleic acid libraries are translated. Further described herein are cell libraries expressing variegated nucleic acid libraries described herein.

公开(公告)号：US12018065B2

公开(公告)日：2024-06-25

申请号：US17242170

申请日：2021-04-27

Applicant: Twist Bioscience Corporation

Inventor： Aaron Sato ,  Qiang Liu ,  Tom Yuan

IPC: C07K16/10 ,  C07K16/00 ,  A61K39/00

CPC classification number: C07K16/10 ,  C07K16/005 ,  A61K2039/505 ,  C07K2317/565 ,  C07K2317/76 ,  C07K2317/92

Abstract: Provided herein are methods and compositions relating to libraries of optimized antibodies having nucleic acids encoding for an antibody comprising modified sequences. Libraries described herein comprise nucleic acids encoding SARS-CoV-2 or ACE2 antibodies. Further described herein are protein libraries generated when the nucleic acid libraries are translated. Further described herein are cell libraries expressing variegated nucleic acid libraries described herein.

公开(公告)号：US11492727B2

公开(公告)日：2022-11-08

申请号：US16802423

申请日：2020-02-26

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： Ray Tabibiazar ,  Aaron Sato ,  Pankaj Garg ,  Qiang Liu ,  Fumiko Axelrod

IPC: C40B40/08 ,  C07K16/28

Abstract: Provided herein are methods and compositions relating to glucagon-like peptide-1 receptor (GLP1R) libraries having nucleic acids encoding for a scaffold comprising a GLP1R binding domain. Libraries described herein include variegated libraries comprising nucleic acids each encoding for a predetermined variant of at least one predetermined reference nucleic acid sequence. Further described herein are protein libraries generated when the nucleic acid libraries are translated. Further described herein are cell libraries expressing variegated nucleic acid libraries described herein.

公开(公告)号：US12201857B2

公开(公告)日：2025-01-21

申请号：US17847104

申请日：2022-06-22

Applicant: Twist Bioscience Corporation

Inventor： Aaron Sato ,  Qiang Liu ,  Tom Yuan

IPC: A61P31/14 ,  A61K39/00 ,  C07K16/10

Abstract: Provided herein are methods and compositions relating to libraries of optimized antibodies having nucleic acids encoding for an antibody comprising modified sequences. Libraries described herein comprise nucleic acids encoding SARS-CoV-2 or ACE2 antibodies. Further described herein are protein libraries generated when the nucleic acid libraries are translated. Further described herein are cell libraries expressing variegated nucleic acid libraries described herein.

公开(公告)号：US12091777B2

公开(公告)日：2024-09-17

申请号：US17030216

申请日：2020-09-23

Applicant: Twist Bioscience Corporation

Inventor： Ray Tabibiazar ,  Aaron Sato ,  Pankaj Garg ,  Qiang Liu

IPC: C40B40/10 ,  C07K16/28 ,  C12N15/10 ,  C40B40/08

CPC classification number: C40B40/10 ,  C07K16/2896 ,  C12N15/1044 ,  C40B40/08 ,  C07K2317/24 ,  C07K2317/565 ,  C07K2317/92

Abstract: Provided herein are methods and compositions relating to prostaglandin D2 receptor 2 (DP2 or CRTH2R) libraries having nucleic acids encoding for a scaffold comprising a CRTH2R binding domain. CRTH2R libraries described herein encode for immunoglobulins including antibodies and single domain antibodies. Libraries described herein include variegated libraries comprising nucleic acids each encoding for a predetermined variant of at least one predetermined reference nucleic acid sequence. Further described herein are protein libraries generated when the nucleic acid libraries are translated. Further described herein are cell libraries expressing variegated nucleic acid libraries described herein.

公开(公告)号：US12202905B2

公开(公告)日：2025-01-21

申请号：US17580547

申请日：2022-01-20

Applicant: Twist Bioscience Corporation

Inventor： Aaron Sato ,  Qiang Liu ,  Fumiko Axelrod ,  Linya Wang

IPC: C07K16/28

Abstract: Provided herein are methods and compositions relating to adenosine A2A receptor libraries having nucleic acids encoding for a scaffold comprising an adenosine A2A binding domain. adenosine A2A receptor libraries described herein encode for immunoglobulins such as antibodies.

公开(公告)号：US11492728B2

公开(公告)日：2022-11-08

申请号：US16802439

申请日：2020-02-26

Applicant: TWIST BIOSCIENCE CORPORATION

Inventor： Aaron Sato

IPC: C40B40/08 ,  G16B35/10 ,  G16B35/20 ,  C07K16/00 ,  C12N15/10

Abstract: Provided herein are methods and compositions relating to libraries of optimized antibodies having nucleic acids encoding for an antibody comprising modified sequences. Libraries described herein include variegated libraries comprising nucleic acids each encoding for a predetermined variant of at least one predetermined reference nucleic acid sequence. Further described herein are protein libraries generated when the nucleic acid libraries are translated. Further described herein are cell libraries expressing variegated nucleic acid libraries described herein.

公开(公告)号：US12134656B2

公开(公告)日：2024-11-05

申请号：US18056679

申请日：2022-11-17

Applicant: Twist Bioscience Corporation

Inventor： Aaron Sato ,  Tom Yuan ,  Linya Wang ,  Fumiko Axelrod

IPC: C07K16/32

Abstract: Provided herein are methods and compositions relating to libraries of optimized antibodies having nucleic acids encoding for an antibody comprising modified sequences. Libraries described herein comprise nucleic acids encoding Dickkopf WNT signaling pathway inhibitor 1 (DKK1) antibodies. Further described herein are protein libraries generated when the nucleic acid libraries are translated. Further described herein are cell libraries expressing variegated nucleic acid libraries described herein.