知搜-全球专利检索

  1. 登录
  2. 注册

搜索结果

377 条记录 (耗时 0.053 秒)

    Kits for analysis using nucleic acid encoding and/or label
    1.
    发明授权
    Kits for analysis using nucleic acid encoding and/or label 有权

    公开(公告)号：US12130291B2

    公开(公告)日：2024-10-29

    申请号：US17819263

    申请日：2022-08-11

    申请人： Encodia, Inc.

    发明人： Mark S. Chee ,  John M. Beierle ,  Norihito Muranaka ,  Kevin L. Gunderson ,  Michael Phillip Weiner ,  Lei Shi ,  Robert C. James ,  Luca Monfregola

    IPC分类号： G01N33/68 ,  C40B40/10 ,  C40B70/00

    CPC分类号： G01N33/6842 ,  C40B40/10 ,  C40B70/00 ,  G01N33/6845

    摘要： Kits and methods of using the kits for analyzing macromolecules, including peptides, polypeptides, and proteins, employing nucleic acid encoding are disclosed. The sample analysis kits employ nucleic acid encoding and/or nucleic acid recording of a molecular interaction and/or reaction, such as recognition events (e.g., between an antigen and an antibody, between a modified terminal amino acid residue, or between a small molecule or peptide therapeutic and a target, etc.). Additional barcoding reagents, such as those for cycle-specific barcoding (e.g., “clocking”), compartment barcoding, combinatorial barcoding, spatial barcoding, or any combination thereof, may be included in the kits. The sample may comprise macromolecules, including peptides, polypeptides, and proteins, and the recording may generate molecular interaction and/or reaction information, and/or polypeptide sequence information. The kits may be used in high-throughput, multiplexed, and/or automated analysis, and are suitable for analysis of a proteome or subset thereof.

    SYSTEMS AND METHODS FOR HIGH-THROUGHPUT IMAGE-BASED SCREENING
    4.
    发明公开
    SYSTEMS AND METHODS FOR HIGH-THROUGHPUT IMAGE-BASED SCREENING 审中-公开

    公开(公告)号：US20240175081A1

    公开(公告)日：2024-05-30

    申请号：US18461672

    申请日：2023-09-06

    申请人： President and Fellows of Harvard College

    发明人： Xiaowei Zhuang ,  George Alexander Emanuel ,  Jeffrey R. Moffitt

    IPC分类号： C12Q1/6841 ,  C40B30/04 ,  C40B70/00

    CPC分类号： C12Q1/6841 ,  C40B30/04 ,  C40B70/00 ,  C12Q2525/161 ,  C12Q2563/179 ,  C12Q2565/514

    摘要： The present invention generally relates to imaging cells, for example, to determine phenotypes and/or genotypes in populations of cells. In some aspects, cells may be analyzed, e.g., imaged, to determine their phenotype, and their genotypes may be determined by exposing the cells to nucleic acid probes, e.g., as in smFISH. MERFISH, FISH, in situ hybridization, or other suitable techniques. In some cases, the cells may be exposed to a nucleic acid comprising an identification portion, which may be used to distinguish the cells from each other. In some embodiments, the cells may be exposed to a nucleic acid comprising an expression portion, e.g. a gene, or coding region for a non-translated RNA, etc., that when expressed, produces a protein, RNA, DNA, or the like that may alter the phenotype of the cell or the variable nucleic acid sequence can consist of promoters, gene regulatory elements, transcription factor binding sites, Cas9 guide RNA coding regions, etc. that otherwise alter the phenotype of the cell. In some embodiments, the modifications that contain either the identification portion, the expression portion, or both may be introduced into the genome of a host organism or as exogenous materials, e.g. plasmids. Such changes may involve the addition of synthetic materials, such as synthetic nucleic acids, or modifications, e.g. deletions or mutations, of the genomic material of the host organism. Other aspects are generally directed to compositions or devices for use in such methods, kits for use in such methods, or the like.

    Kits for analysis using nucleic acid encoding and/or label
    6.
    发明授权
    Kits for analysis using nucleic acid encoding and/or label 有权

    公开(公告)号：US11782062B2

    公开(公告)日：2023-10-10

    申请号：US18166461

    申请日：2023-02-08

    申请人： Encodia, Inc.

    发明人： Mark S. Chee ,  John M. Beierle ,  Norihito Muranaka ,  Kevin L. Gunderson ,  Michael Phillip Weiner ,  Lei Shi ,  Robert C. James ,  Luca Monfregola

    IPC分类号： C40B70/00 ,  G01N33/68 ,  C40B40/10

    CPC分类号： G01N33/6842 ,  C40B40/10 ,  C40B70/00 ,  G01N33/6845

    摘要： Kits and methods of using the kits for analyzing macromolecules, including peptides, polypeptides, and proteins, employing nucleic acid encoding are disclosed. The sample analysis kits employ nucleic acid encoding and/or nucleic acid recording of a molecular interaction and/or reaction, such as recognition events (e.g., between an antigen and an antibody, between a modified terminal amino acid residue, or between a small molecule or peptide therapeutic and a target, etc.). Additional barcoding reagents, such as those for cycle-specific barcoding (e.g., “clocking”), compartment barcoding, combinatorial barcoding, spatial barcoding, or any combination thereof, may be included in the kits. The sample may comprise macromolecules, including peptides, polypeptides, and proteins, and the recording may generate molecular interaction and/or reaction information, and/or polypeptide sequence information. The kits may be used in high-throughput, multiplexed, and/or automated analysis, and are suitable for analysis of a proteome or subset thereof.

    Kits for analysis using nucleic acid encoding and/or label
    9.
    发明授权
    Kits for analysis using nucleic acid encoding and/or label 有权

    公开(公告)号：US11513126B2

    公开(公告)日：2022-11-29

    申请号：US16760028

    申请日：2018-10-31

    申请人： Encodia, Inc.

    发明人： Mark S. Chee ,  John M. Beierle ,  Norihito Muranaka ,  Kevin L. Gunderson ,  Michael Phillip Weiner ,  Lei Shi ,  Robert C. James ,  Luca Monfregola

    IPC分类号： C12Q1/6804 ,  G01N33/68 ,  C40B40/10 ,  C40B70/00

    摘要： Kits and methods of using the kits for analyzing macromolecules, including peptides, polypeptides, and proteins, employing nucleic acid encoding are disclosed. The sample analysis kits employ nucleic acid encoding and/or nucleic acid recording of a molecular interaction and/or reaction, such as recognition events (e.g., between an antigen and an antibody, between a modified terminal amino acid residue, or between a small molecule or peptide therapeutic and a target, etc.). Additional barcoding reagents, such as those for cycle-specific barcoding (e.g., “clocking”), compartment barcoding, combinatorial barcoding, spatial barcoding, or any combination thereof, may be included in the kits. The sample may comprise macromolecules, including peptides, polypeptides, and proteins, and the recording may generate molecular interaction and/or reaction information, and/or polypeptide sequence information. The kits may be used in high-throughput, multiplexed, and/or automated analysis, and are suitable for analysis of a proteome or subset thereof.