公开(公告)号：US20240254658A1

公开(公告)日：2024-08-01

申请号：US18593788

申请日：2024-03-01

申请人： 10x Genomics, Inc.

发明人： Jason Bell ,  Francesca Meschi ,  Geoffrey McDermott ,  Michael Schnall-Levin ,  Xinying Zheng

IPC分类号： C40B30/04 ,  C07K14/74 ,  C12N15/10 ,  C12N15/11 ,  C12N15/85 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  C40B50/06 ,  C40B70/00 ,  G01N33/50 ,  G01N33/53 ,  G01N33/532 ,  G01N33/543 ,  G01N33/548 ,  G01N33/569 ,  G01N33/58

CPC分类号： C40B30/04 ,  C07K14/70539 ,  C12N15/1037 ,  C12N15/1055 ,  C12N15/1065 ,  C12N15/1075 ,  C12N15/11 ,  C12N15/85 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  G01N33/5032 ,  G01N33/505 ,  G01N33/5304 ,  G01N33/5306 ,  G01N33/5308 ,  G01N33/532 ,  G01N33/54306 ,  G01N33/54366 ,  G01N33/548 ,  G01N33/56977 ,  G01N33/58 ,  C12N2310/20 ,  C12N2320/10 ,  C12Q2537/164 ,  C12Q2563/179 ,  C12Q2563/185 ,  C12Q2565/1015 ,  C40B50/06 ,  C40B70/00

摘要： Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

公开(公告)号：US10725027B2

公开(公告)日：2020-07-28

申请号：US16375093

申请日：2019-04-04

申请人： 10X Genomics, Inc.

发明人： Jason Bell ,  Geoffrey McDermott ,  Francesca Meschi ,  Michael Schnall-Levin ,  Xinying Zheng

IPC分类号： G01N33/532 ,  G01N33/543 ,  G01N33/548 ,  C12N15/10 ,  C12Q1/6804 ,  C12Q1/6806 ,  G01N33/50 ,  G01N33/53 ,  G01N33/58 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  C12N15/11 ,  G01N33/569 ,  C40B70/00 ,  C07K14/74 ,  C12N15/85

摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from a single cell. Such polynucleotide processing may be useful for a variety of applications. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins) and chromatin (e.g., accessible chromatin).

公开(公告)号：US20190383804A1

公开(公告)日：2019-12-19

申请号：US16375119

申请日：2019-04-04

申请人： 10X Genomics, Inc.

发明人： Kamila Belhocine ,  Jason Bell ,  Zachary Bent ,  Rajiv Bharadwaj ,  Christopher Hindson ,  Mohammad Rahimi Lenji ,  Bill Kengli Lin ,  Anthony Makarewicz ,  Geoffrey McDermott ,  Elliott Meer ,  Francesca Meschi ,  Tarjei Sigurd Mikkelsen ,  Christopher Joachim O'Keeffe ,  Katherine Pfeiffer ,  Andrew D. Price ,  Paul Ryvkin ,  Michael Schnall-Levin ,  Sarah Taylor ,  Jessica Michele Terry ,  Tobias Daniel Wheeler ,  Yifeng Yin ,  Xinying Zheng ,  Solongo Batjargal Ziraldo ,  Eswar Prasad Ramachandran Iyer ,  Luigi Jhon Alvarado Martinez

IPC分类号： G01N33/53 ,  G01N33/543 ,  C12N15/11

摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from a single cell. Such polynucleotide processing may be useful for a variety of applications. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, epigenetic information (e.g., accessible chromatin, DNA methylation), and RNA molecules (e.g., mRNA or CRISPR guide RNAs). In some cases, the disclosed methods comprise analysis of analytes from a cell using a cell bead.

公开(公告)号：US20190367969A1

公开(公告)日：2019-12-05

申请号：US16375093

申请日：2019-04-04

申请人： 10X Genomics, Inc.

发明人： Kamila Belhocine ,  Jason Bell ,  Zachary Bent ,  Rajiv Bharadwaj ,  Christopher Hindson ,  Mohammad Rahimi Lenji ,  Bill Kengli Lin ,  Anthony Makarewicz ,  Geoffrey McDermott ,  Elliott Meer ,  Francesca Meschi ,  Tarjei Sigurd Mikkelsen ,  Christopher Joachim O'Keeffe ,  Katherine Pfeiffer ,  Andrew D. Price ,  Paul Ryvkin ,  Michael Schnall-Levin ,  Sarah Taylor ,  Jessica Michele Terry ,  Tobias Daniel Wheeler ,  Yifeng Yin ,  Xinying Zheng ,  Solongo Batjargal Ziraldo ,  Eswar Prasad Ramachandran Iyer ,  Luigi Jhon Alvarado Martinez

IPC分类号： C12Q1/6818 ,  C12Q1/6827

摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from a single cell. Such polynucleotide processing may be useful for a variety of applications. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins) and chromatin (e.g., accessible chromatin).

公开(公告)号：US11739440B2

公开(公告)日：2023-08-29

申请号：US16897126

申请日：2020-06-09

申请人： 10X Genomics, Inc.

发明人： Jason Bell ,  Geoffrey McDermott ,  Francesca Meschi ,  Katherine Pfeiffer ,  Michael Schnall-Levin ,  Xinying Zheng

IPC分类号： C40B30/04 ,  C12N15/85 ,  G01N33/532 ,  G01N33/543 ,  G01N33/548 ,  C12N15/10 ,  C12Q1/6806 ,  G01N33/50 ,  G01N33/53 ,  G01N33/58 ,  C12N15/11 ,  C40B70/00 ,  C40B50/06 ,  C07K14/74 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  G01N33/569

CPC分类号： C40B30/04 ,  C07K14/70539 ,  C12N15/1037 ,  C12N15/1055 ,  C12N15/1065 ,  C12N15/1075 ,  C12N15/11 ,  C12N15/85 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  G01N33/505 ,  G01N33/5032 ,  G01N33/532 ,  G01N33/5304 ,  G01N33/5306 ,  G01N33/5308 ,  G01N33/548 ,  G01N33/54306 ,  G01N33/54366 ,  G01N33/56977 ,  G01N33/58 ,  C12N2310/20 ,  C12N2320/10 ,  C12Q2537/164 ,  C12Q2563/179 ,  C12Q2563/185 ,  C12Q2565/1015 ,  C40B50/06 ,  C40B70/00

摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from a single cell. Such polynucleotide processing may be useful for a variety of applications. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins) and chromatin (e.g., accessible chromatin).

公开(公告)号：US20220259586A1

公开(公告)日：2022-08-18

申请号：US17492536

申请日：2021-10-01

申请人： 10X GENOMICS, INC.

发明人： Zahra Kamila Belhocine ,  Geoffrey McDermott ,  Francesca Meschi ,  Xinying Zheng

IPC分类号： C12N15/10

摘要： Methods and systems for sample preparation techniques that allow amplification (e.g., whole genome amplification) and sequencing of chromatin accessible regions of single cells are provided. The methods and systems generally operate by forming or providing partitions (e.g., droplets) including a single biological particle and a single bead comprising a barcoded oligonucleotide. The preparation of barcoded next-generation sequencing libraries prepared from a single cell is facilitated by the transposon-mediated transposition and fragmentation of a target nucleic acid sequence. The methods and systems may be configured to allow the implementation of single-operation or multi-operation chemical and/or biochemical processing within the partitions.

公开(公告)号：US10927370B2

公开(公告)日：2021-02-23

申请号：US16419630

申请日：2019-05-22

申请人： 10X GENOMICS, INC.

发明人： Zahra Kamila Belhocine ,  Geoffrey McDermott ,  Francesca Meschi ,  Xinying Zheng

IPC分类号： C12N15/10

摘要： Methods and systems for sample preparation techniques that allow amplification (e.g., whole genome amplification) and sequencing of chromatin accessible regions of single cells are provided. The methods and systems generally operate by forming or providing partitions (e.g., droplets) including a single biological particle and a single bead comprising a barcoded oligonucleotide. The preparation of barcoded next-generation sequencing libraries prepared from a single cell is facilitated by the transposon-mediated transposition and fragmentation of a target nucleic acid sequence. The methods and systems may be configured to allow the implementation of single-operation or multi-operation chemical and/or biochemical processing within the partitions.

公开(公告)号：US20200263232A1

公开(公告)日：2020-08-20

申请号：US16530930

申请日：2019-08-02

申请人： 10X GENOMICS, INC.

发明人： Jason Bell ,  Josephine Lee ,  Corey Nemec ,  Francesca Meschi

IPC分类号： C12Q1/6806 ,  C12N9/12 ,  C12N15/10

摘要： Methods and compositions to minimize barcode exchange during the preparation of barcoded next-generation sequencing libraries prepared from a single cell. The methods utilize oligonucleotides containing a 3′-terminated blocking group or sequences that prevent amplification or extension.

公开(公告)号：US20180340170A1

公开(公告)日：2018-11-29

申请号：US15842687

申请日：2017-12-14

申请人： 10X Genomics, Inc.

发明人： Kamila Belhocine ,  Geoffrey McDermott ,  Francesca Meschi ,  Xinying Zheng

IPC分类号： C12N15/10

CPC分类号： C12N15/1065 ,  C12N15/1068 ,  C12N15/1075 ,  C12N15/1096 ,  C12Q2563/179

摘要： Methods and systems for sample preparation techniques that allow amplification (e.g., whole genome amplification) and sequencing of chromatin accessible regions of single cells are provided. The methods and systems generally operate by forming or providing partitions (e.g., droplets) including a single biological particle and a single bead comprising a barcoded oligonucleotide. The preparation of barcoded next-generation sequencing libraries prepared from a single cell is facilitated by the transposon-mediated transposition and fragmentation of a target nucleic acid sequence. The methods and systems may be configured to allow the implementation of single-operation or multi-operation chemical and/or biochemical processing within the partitions.

公开(公告)号：US12049712B2

公开(公告)日：2024-07-30

申请号：US17229859

申请日：2021-04-13

申请人： 10X Genomics, Inc.

发明人： Jason Bell ,  Francesca Meschi ,  Geoffrey McDermott ,  Michael Schnall-Levin ,  Xinying Zheng

IPC分类号： C40B30/04 ,  C07K14/74 ,  C12N15/10 ,  C12N15/11 ,  C12N15/85 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  C40B50/06 ,  C40B70/00 ,  G01N33/50 ,  G01N33/53 ,  G01N33/532 ,  G01N33/543 ,  G01N33/548 ,  G01N33/569 ,  G01N33/58

CPC分类号： C40B30/04 ,  C07K14/70539 ,  C12N15/1037 ,  C12N15/1055 ,  C12N15/1065 ,  C12N15/1075 ,  C12N15/11 ,  C12N15/85 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  G01N33/5032 ,  G01N33/505 ,  G01N33/5304 ,  G01N33/5306 ,  G01N33/5308 ,  G01N33/532 ,  G01N33/54306 ,  G01N33/54366 ,  G01N33/548 ,  G01N33/56977 ,  G01N33/58 ,  C12N2310/20 ,  C12N2320/10 ,  C12Q2537/164 ,  C12Q2563/179 ,  C12Q2563/185 ,  C12Q2565/1015 ,  C40B50/06 ,  C40B70/00

摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from a single cell. Such polynucleotide processing may be useful for a variety of applications. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins) and chromatin (e.g., accessible chromatin).