公开(公告)号：US11815508B2

公开(公告)日：2023-11-14

申请号：US16508459

申请日：2019-07-11

Applicant: Dana-Farber Cancer Institute, Inc.

Inventor： Anthony Letai ,  Juan Jose Montero Boronat ,  Jeremy Ryan

IPC: G01N33/50 ,  G01N33/574 ,  G01N33/569

CPC classification number: G01N33/5011 ,  G01N33/5008 ,  G01N33/5014 ,  G01N33/5032 ,  G01N33/5079 ,  G01N33/56972 ,  G01N33/5743 ,  G01N33/57419 ,  G01N33/57423 ,  G01N33/57426 ,  G01N33/5023 ,  G01N33/5094 ,  G01N2800/52

Abstract: The present invention provides methods of predicting cell sensitivity or resistance to a therapeutic agent.

公开(公告)号：US20230341378A1

公开(公告)日：2023-10-26

申请号：US18214651

申请日：2023-06-27

Applicant: EMULATE, Inc.

Inventor： Daniel Levner ,  Kyung Jin Jang ,  Jacob Fraser ,  S. Jordan Kerns ,  Antonio Varone ,  Dongeun Huh

IPC: G01N33/50 ,  C12N5/071 ,  C12M1/12 ,  C12N5/077 ,  C12N5/00 ,  C07K2/00 ,  C07K14/78 ,  C12M3/06 ,  C12M1/00

CPC classification number: G01N33/5032 ,  C07K2/00 ,  C07K14/78 ,  C12M23/16 ,  C12M23/20 ,  C12M25/02 ,  C12N5/0018 ,  C12N5/0068 ,  C12N5/0658 ,  C12N5/067 ,  G01N33/5014 ,  C12N2500/32 ,  C12N2521/00 ,  C12N2533/30 ,  C12N2533/50 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/90 ,  C12N2535/10 ,  C12N2537/10 ,  G01N33/5044

Abstract: Compositions, devices and methods are described for improving adhesion, attachment, and/or differentiation of cells in a microfluidic device or chip. In one embodiment, one or more ECM proteins are covalently coupled to the surface of a microchannel of a microfluidic device. The microfluidic devices can be stored or used immediately for culture and/or support of living cells such as mammalian cells, and/or for simulating a function of a tissue, e.g., a liver tissue, muscle tissue, etc. Extended adhesion and viability with sustained function over time is observed.

公开(公告)号：US11739440B2

公开(公告)日：2023-08-29

申请号：US16897126

申请日：2020-06-09

Applicant: 10X Genomics, Inc.

Inventor： Jason Bell ,  Geoffrey McDermott ,  Francesca Meschi ,  Katherine Pfeiffer ,  Michael Schnall-Levin ,  Xinying Zheng

IPC: C40B30/04 ,  C12N15/85 ,  G01N33/532 ,  G01N33/543 ,  G01N33/548 ,  C12N15/10 ,  C12Q1/6806 ,  G01N33/50 ,  G01N33/53 ,  G01N33/58 ,  C12N15/11 ,  C40B70/00 ,  C40B50/06 ,  C07K14/74 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  G01N33/569

CPC classification number: C40B30/04 ,  C07K14/70539 ,  C12N15/1037 ,  C12N15/1055 ,  C12N15/1065 ,  C12N15/1075 ,  C12N15/11 ,  C12N15/85 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  G01N33/505 ,  G01N33/5032 ,  G01N33/532 ,  G01N33/5304 ,  G01N33/5306 ,  G01N33/5308 ,  G01N33/548 ,  G01N33/54306 ,  G01N33/54366 ,  G01N33/56977 ,  G01N33/58 ,  C12N2310/20 ,  C12N2320/10 ,  C12Q2537/164 ,  C12Q2563/179 ,  C12Q2563/185 ,  C12Q2565/1015 ,  C40B50/06 ,  C40B70/00

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from a single cell. Such polynucleotide processing may be useful for a variety of applications. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins) and chromatin (e.g., accessible chromatin).

公开(公告)号：US20230236175A1

公开(公告)日：2023-07-27

申请号：US17820560

申请日：2022-08-17

Applicant: 10x Genomics, Inc.

Inventor： Zahra Kamila Belhocine ,  Jason Bell ,  Jerald Sapida ,  Katrina Sullivan-Bibee

IPC: G01N33/532 ,  C07K14/74 ,  C12N15/85 ,  G01N33/543 ,  G01N33/548 ,  C12N15/10 ,  C12Q1/6804 ,  C12Q1/6806 ,  G01N33/50 ,  G01N33/53 ,  G01N33/58 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  C12N15/11 ,  G01N33/569

CPC classification number: G01N33/532 ,  C07K14/70539 ,  C12N15/85 ,  G01N33/54306 ,  G01N33/548 ,  C12N15/1037 ,  C12N15/1075 ,  C12Q1/6804 ,  C12Q1/6806 ,  G01N33/505 ,  G01N33/5308 ,  G01N33/58 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  C12N15/11 ,  G01N33/5304 ,  G01N33/5306 ,  C12N15/1055 ,  C12N15/1065 ,  G01N33/5032 ,  G01N33/54366 ,  G01N33/56977 ,  C12Q2537/164 ,  C12Q2565/1015 ,  C12Q2563/179 ,  C12Q2563/185 ,  C12N2310/20 ,  C40B70/00

Abstract: The present disclosure provides methods, systems, and kits for processing nucleic acid molecules. A method may comprise providing a template nucleic acid fragment (e.g., within a cell, cell bead, or cell nucleus) within a partition (e.g., a droplet or well) and subjecting the template nucleic acid fragment to one or more processes including a barcoding process and a single primer extension or amplification process. The processed template nucleic acid fragment may then be recovered from the partition and subjected to further amplification to provide material for subsequent sequencing analysis. The methods provided herein may permit simultaneous processing and analysis of both DNA and RNA molecules originating from the same cell, cell bead, or cell nucleus.

公开(公告)号：US20180179278A1

公开(公告)日：2018-06-28

申请号：US15891802

申请日：2018-02-08

Applicant: Board of Regents of the University of Texas System

Inventor： Jean X. Jiang ,  Manuel A. Riquelme ,  Sumin Gu

IPC: C07K16/28 ,  G01N33/50 ,  A61K49/00

CPC classification number: C07K16/28 ,  A61K49/0008 ,  C07K2317/76 ,  G01N33/5032

Abstract: Methods for identifying compounds that positively regulate connexin 43 hemichannels.

公开(公告)号：US20180066220A1

公开(公告)日：2018-03-08

申请号：US15697306

申请日：2017-09-06

Applicant: Pulak Nath ,  Jen-Huang Huang

Inventor： Pulak Nath ,  Jen-Huang Huang

IPC: C12M3/06 ,  C12M1/00 ,  C12M1/12 ,  C12M3/00 ,  C12N5/071 ,  G01N33/50 ,  C12M1/42 ,  C12M1/34

CPC classification number: C12M23/16 ,  C12M21/08 ,  C12M23/26 ,  C12M25/10 ,  C12M25/14 ,  C12M29/10 ,  C12M33/12 ,  C12M35/04 ,  C12M35/08 ,  C12M41/40 ,  C12N5/0697 ,  C12N2502/095 ,  C12N2502/1305 ,  C12N2502/1323 ,  C12N2502/27 ,  C12N2502/28 ,  C12N2502/30 ,  C12N2521/00 ,  C12N2533/78 ,  G01N33/5032

Abstract: Devices and systems for cell culture that include one or more hollow fibers or channels integrated into a chamber are provided. The hollow fibers or channels and/or the chamber are seeded with one or more cell types. Methods of co-culturing two or more cell types in the devices are also provided.

公开(公告)号：US20180024119A1

公开(公告)日：2018-01-25

申请号：US15648319

申请日：2017-07-12

Applicant: Emulate Inc.

Inventor： Daniel Levner ,  Kyung Jin Jang ,  Jacob Fraser ,  Jordan Kerns ,  Antonio Varone ,  Dongeun Huh

IPC: G01N33/50 ,  C12N5/071 ,  C12N5/00

CPC classification number: G01N33/5032 ,  C12M23/16 ,  C12M23/20 ,  C12M25/02 ,  C12N5/0018 ,  C12N5/0068 ,  C12N5/0658 ,  C12N5/067 ,  C12N2500/32 ,  C12N2521/00 ,  C12N2533/30 ,  C12N2533/50 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/90 ,  C12N2535/10 ,  C12N2537/10 ,  G01N33/5014 ,  G01N33/5044

Abstract: Compositions, devices and methods are described for improving adhesion, attachment, and/or differentiation of cells in a microfluidic device or chip. In one embodiment, one or more ECM proteins are covalently coupled to the surface of a microchannel of a microfluidic device. The microfluidic devices can be stored or used immediately for culture and/or support of living cells such as mammalian cells, and/or for simulating a function of a tissue, e.g., a liver tissue, muscle tissue, etc. Extended adhesion and viability with sustained function over time is observed.

公开(公告)号：US09493555B2

公开(公告)日：2016-11-15

申请号：US15071068

申请日：2016-03-15

Applicant: The Board of Trustees of the Leland Stanford Junior University

Inventor： Ben A. Barres ,  Beth A. Stevens

IPC: C07K16/18 ,  A61K31/00 ,  A61K31/727 ,  A61K45/06 ,  G01N33/50 ,  G01N33/564 ,  G01N33/68 ,  A61K39/395 ,  A61K48/00 ,  A61K39/00

CPC classification number: G01N33/5058 ,  A61K31/00 ,  A61K31/727 ,  A61K39/3955 ,  A61K45/06 ,  A61K48/00 ,  A61K2039/505 ,  C07K16/18 ,  C07K2317/76 ,  G01N33/5032 ,  G01N33/564 ,  G01N33/6872 ,  G01N33/6896 ,  G01N2333/4716 ,  G01N2500/04 ,  G01N2800/28 ,  G01N2800/2821 ,  A61K2300/00

Abstract: C1q is shown to be expressed in neurons, where it acts as a signal for synapse elimination. Methods are provided for protecting or treating an individual suffering from adverse effects of synapse loss. These findings have broad implications for a variety of clinical conditions, including Alzheimer's disease.

公开(公告)号：US09382313B2

公开(公告)日：2016-07-05

申请号：US14751921

申请日：2015-06-26

Applicant: The Board of Trustees of the Leland Stanford Junior University

Inventor： Ben A. Barres ,  Beth A. Stevens

IPC: C07K16/18 ,  A61K31/00 ,  A61K31/727 ,  A61K45/06 ,  G01N33/50 ,  G01N33/564 ,  G01N33/68 ,  A61K39/395 ,  A61K48/00 ,  A61K39/00

CPC classification number: G01N33/5058 ,  A61K31/00 ,  A61K31/727 ,  A61K39/3955 ,  A61K45/06 ,  A61K48/00 ,  A61K2039/505 ,  C07K16/18 ,  C07K2317/76 ,  G01N33/5032 ,  G01N33/564 ,  G01N33/6872 ,  G01N33/6896 ,  G01N2333/4716 ,  G01N2500/04 ,  G01N2800/28 ,  G01N2800/2821 ,  A61K2300/00

Abstract: C1q is shown to be expressed in neurons, where it acts as a signal for synapse elimination. Methods are provided for protecting or treating an individual suffering from adverse effects of synapse loss. These findings have broad implications for a variety of clinical conditions, including Alzheimer's disease.

公开(公告)号：US20160061819A1

公开(公告)日：2016-03-03

申请号：US14835834

申请日：2015-08-26

Applicant: Leibniz - Institut fuer Analytische Wissenschaften - ISAS - e.V.

Inventor： Jonathan WEST ,  Dirk JANASEK ,  Albert SICKMANN

IPC: G01N33/50

CPC classification number: G01N33/5032 ,  G01N15/10 ,  G01N27/447 ,  G01N33/4915 ,  G01N2015/1006

Abstract: With a method for measurement of thrombocyte function, a solution is created, by which the sensitivity of individual thrombocytes can be measured with the least possible apparatus effort, with high throughput, by passing a liquid thrombocyte solution, in which the thrombocytes are present in isolated form, into a microfluidic chamber and brought into contact with at least one stimulant, wherein an electrical field directed transverse to the entry direction of the thrombocyte solution is applied to the chamber, and the movement path of the thrombocytes in the electrical field is observed and evaluated, in such a manner that thrombocytes having a movement path directed in the direction toward the minus pole of the electrical field are classified as non-activated thrombocytes, and thrombocytes having a movement path directed in the direction toward the plus pole of the electrical field are classified as activated thrombocytes.

Abstract translation: 使用测定血小板功能的方法，产生溶液，通过使溶液中存在分离的血小板溶液，可以以最小可能的装置努力以高通量测量各个凝血细胞的灵敏度 形成微流体室并与至少一种兴奋剂接触，其中横向于血小板溶液进入方向的电场被施加到腔室，并且观察到电场中血小板的移动路径， 评估，使得具有朝向电场负极的方向的移动路径的血小板被分类为非活化血小板，并且具有朝向电场的正极的方向的移动路径的血小板细胞 被分类为活化血小板。