-
公开(公告)号：US12091650B2
公开(公告)日：2024-09-17
申请号：US16286185
申请日：2019-02-26
发明人： S. Jordan Kerns , Norman Wen , Carolina Lucchesi , Christopher David Hinojosa , Jacob Fraser , Geraldine Hamilton , Gad Vatine , Samuel Sances , Clive Svendsen , Daniel Levner , Dhruv Sareen
CPC分类号： C12M23/16 , B01L3/502715 , C12M25/02 , C12N5/0068 , C12N5/0619 , C12N5/0622 , C12N5/069 , B01L2200/0647 , B01L2300/0861 , C12N2502/081 , C12N2502/28 , C12N2506/02 , C12N2506/45 , C12N2531/00 , C12N2539/00
摘要： The invention relates to culturing brain endothelial cells, and optionally astrocytes and neurons in a fluidic device under conditions whereby the cells mimic the structure and function of the blood brain barrier. Culture of such cells in a microfluidic device, whether alone or in combination with other cells, drives maturation and/or differentiation further than existing systems.
-
公开(公告)号：US20240093136A1
公开(公告)日：2024-03-21
申请号：US18520821
申请日：2023-11-28
申请人： EMULATE, Inc.
发明人： Daniel Levner , Josiah Daniel Sliz , Christopher Davidd Hinojosa , Joshua Gomes , Jose Fernandez-Alcon
IPC分类号： C12M3/06 , A01N1/02 , B01L3/00 , B01L9/00 , C12M1/00 , C12M1/34 , C12M1/36 , C12M1/42 , C12M3/00 , C12N5/071
CPC分类号： C12M23/16 , A01N1/021 , A01N1/0247 , B01L3/502707 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L9/527 , C12M21/08 , C12M23/38 , C12M23/40 , C12M23/42 , C12M29/10 , C12M35/04 , C12M41/40 , C12M41/48 , C12N5/0602 , B01L2200/025 , B01L2200/027 , B01L2200/12 , B01L2300/0681 , B01L2300/0887 , B01L2300/123 , B01L2300/14 , B01L2300/161 , B01L2300/165 , B01L2400/0481 , B01L2400/0487 , B01L2400/06 , C12N2521/00
摘要： A culture module is contemplated that allows the perfusion and optionally mechanical actuation of one or more microfluidic devices, such as organ-on-a-chip microfluidic devices comprising cells that mimic at least one function of an organ in the body. A method for pressure control is contemplated to allow the control of flow rate (while perfusing cells) despite limitations of common pressure regulators. The method for pressure control allows for perfusion of a microfluidic device, such as an organ on a chip microfluidic device comprising cells that mimic cells in an organ in the body, that is detachably linked with said assembly, so that fluid enters ports of the microfluidic device from a fluid reservoir, optionally without tubing, at a controllable flow rate.
-
公开(公告)号：US11833512B2
公开(公告)日：2023-12-05
申请号：US17215900
申请日：2021-03-29
申请人： EMULATE, Inc.
发明人： S. Jordan Kerns , Riccardo Barrile , Geraldine Hamilton , Catherine Karalis , Daniel Levner , Carolina Lucchesi , Antonio Varone , Remi Villenave
CPC分类号： B01L3/502753 , B01L3/502715 , C12M23/16 , C12M29/04 , C12M35/08 , G01N33/5044 , C12N5/0018 , C12N5/0075
摘要： An in vitro microfluidic “organ-on-chip” is described herein that mimics the structure and at least one function of specific areas of the epithelial system in vivo. In particular, a multicellular, layered, microfluidic culture is described, allowing for interactions between lamina propria-derived cells and the associated tissue specific epithelial cells and endothelial cells. This in vitro microfluidic system can be used for modeling inflammatory tissue, e.g., autoimmune disorders involving epithelia and diseases involving epithelial layers. These multicellular, layered microfluidic “organ-on-chip”, e.g. “epithelia-on-chip” further allow for comparisons between types of epithelia tissues, e.g., lung (Lung-On-Chip), bronchial (Airway-On-Chip), skin (Skin-On-Chip), cervix (Cervix-On-Chip), blood brain barrier (BBB-On-Chip), etc., in additional to neurovascular tissue, (Brain-On-Chip), and between different disease states of tissue, i.e. healthy, pre-disease and diseased areas. Additionally, these microfluidic “organ-on-chips” allow identification of cells and cellular derived factors driving disease states in addition to drug testing for reducing inflammation effecting epithelial regions.
-
公开(公告)号：US11733234B2
公开(公告)日：2023-08-22
申请号：US15648339
申请日：2017-07-12
申请人： Emulate Inc.
发明人： Daniel Levner , Kyung Jin Jang , Jacob Fraser , S. Jordan Kerns , Antonio Varone , Dongeun Huh
IPC分类号： C12N5/00 , C12N5/02 , A61K38/00 , C07K2/00 , C07K4/00 , C07K5/00 , C07K7/00 , C07K14/00 , C07K16/00 , C07K17/00 , G01N33/50 , C07K14/78 , C12N5/071 , C12M3/06 , C12M1/00 , C12M1/12 , C12N5/077
CPC分类号： G01N33/5032 , C07K2/00 , C07K14/78 , C12M23/16 , C12M23/20 , C12M25/02 , C12N5/0018 , C12N5/0068 , C12N5/067 , C12N5/0658 , G01N33/5014 , C12N2500/32 , C12N2521/00 , C12N2533/30 , C12N2533/50 , C12N2533/52 , C12N2533/54 , C12N2533/90 , C12N2535/10 , C12N2537/10 , G01N33/5044
摘要： Compositions, devices and methods are described for improving adhesion, attachment, and/or differentiation of cells in a microfluidic device or chip. In one embodiment, one or more ECM proteins are covalently coupled to the surface of a microchannel of a microfluidic device. The microfluidic devices can be stored or used immediately for culture and/or support of living cells such as mammalian cells, and/or for simulating a function of a tissue, e.g., a liver tissue, muscle tissue, etc. Extended adhesion and viability with sustained function over time is observed.
-
公开(公告)号：US20220282221A1
公开(公告)日：2022-09-08
申请号：US17678485
申请日：2022-02-23
发明人： S. Jordan Kerns , Norman Wen , Carol Lucchesi , Christopher David Hinojosa , Jacob Fraser , Jefferson Puerta , Geraldine Hamilton , Robert Barrett , Clive Svendsen , Daniel Levner , Stephen R. Targan , Michael Workman , Dhruv Sareen , Uthra Rajamani , Magdalena Kasendra
摘要： Organs-on-chips are microfluidic devices for culturing living cells in micrometer sized chambers in order to model physiological functions of tissues and organs. Engineered patterning and continuous fluid flow in these devices has allowed culturing of intestinal cells bearing physiologically relevant features and sustained exposure to bacteria while maintaining cellular viability, thereby allowing study of inflammatory bowl diseases. However, existing intestinal cells do not possess all physiologically relevant subtypes, do not possess the repertoire of genetic variations, or allow for study of other important cellular actors such as immune cells. Use of iPSC-derived epithelium, including IBD patient-specific cells, allows for superior disease modeling by capturing the multi-faceted nature of the disease.
-
公开(公告)号：US20220155328A1
公开(公告)日：2022-05-19
申请号：US17538518
申请日：2021-11-30
申请人： EMULATE, Inc.
发明人： JOSIAH SLIZ , Daniel Levner , Brian Zuckerman , Norman Wen , Jonathan Rubins , Tanvi Shroff , Christopher David Hinojosa , Grace Ahn , Victor Antontsev , Jefferson Puerta , David Conegliano , S. Jordan Kerns
摘要： The present invention is related to the field of microfluidics and compound distribution within microfluidic devices and their associated systems. In one embodiment, present invention aims to solve the problem of molecule and compound absorbency into the materials making up laboratory equipment, microfluidic devices and their related infrastructure, without unduly restricting gas transport within microfluidic devices.
-
公开(公告)号：US11248203B2
公开(公告)日：2022-02-15
申请号：US17160617
申请日：2021-01-28
申请人： EMULATE, INC.
发明人： Daniel Levner , Christopher David Hinojosa , Norman Wen , Antonio Varone , Justin Nguyen , Lina Williamson , S. Jordan Kerns , Catherine Karalis , Geraldine Hamilton , Carol Lucchesi
IPC分类号： C12M3/06 , C12M1/12 , C12M1/00 , C12M1/42 , G01N33/50 , G01N33/543 , C12N5/0793 , C12N5/079 , C12N5/071 , C12N5/077
摘要： A microfluidic device is contemplated comprising an open-top cavity with structural anchors on the vertical wall surfaces that serve to prevent gel shrinkage-induced delamination, a porous membrane (optionally stretchable) positioned in the middle over a microfluidic channel(s). The device is particularly suited to the growth of cells mimicking dermal layers.
-
公开(公告)号：US20210340572A1
公开(公告)日：2021-11-04
申请号：US17375324
申请日：2021-07-14
申请人： EMULATE, Inc.
发明人： Kyung-Jin Jang , Daniel Levner , Konstantia-Roumvini Kodella , Jonathan Rubins , Debora Barreiros Petropolis , matt Boeckeler , Geraldine Hamilton
摘要： The present invention is related to high-content microscopy imaging of microfluidic cell culture systems. A method of high-content microfluidic device microscopy is contemplated, along with related statistical analysis and microfluidic device adaptors.
-
公开(公告)号：US10989721B2
公开(公告)日：2021-04-27
申请号：US15648162
申请日：2017-07-12
申请人： Emulate Inc.
发明人： Daniel Levner , Christopher David Hinojosa , Norman Wen , Jacob Fraser , Justin Nguyen , Riccardo Barrile , Geraldine Hamilton , Catherine Karalis , Hyoungshin Park , Antonio Varone , Andries Van der Meer , Monicah Otieno , David Conegliano
摘要： Compositions, devices and methods are described for preventing, reducing, controlling or delaying adhesion, adsorption, surface-mediated clot formation, or coagulation in a microfluidic device or chip. In one embodiment, blood (or other fluid with blood components) that contains anticoagulant is introduced into a microfluidic device comprising one or more additive channels containing one or more reagents that will re-activate the native coagulation cascade in the blood that makes contact with it “on-chip” before moving into the experimental region of the chip.
-
公开(公告)号：US10974242B2
公开(公告)日：2021-04-13
申请号：US15647853
申请日：2017-07-12
申请人： EMULATE, Inc.
发明人： Daniel Levner , Josiah Daniel Sliz , Christopher David Hinojosa , Joshua Gomes , Kyung Jin Jang
摘要： Methods of removing bubbles from a microfluidic device are described where the flow is not stopped. Methods are described that combine pressure and flow to remove bubbles from a microfluidic device. Bubbles can be removed even where the device is made of a polymer that is largely gas impermeable.
-
-
-
-
-
-
-
-
-
搜索结果
104 条记录 (耗时 0.028 秒)
