公开(公告)号：US20240360409A1

公开(公告)日：2024-10-31

申请号：US18292173

申请日：2022-07-29

申请人： TOKYO MEDICAL UNIVERSITY

发明人： Takahiro OCHIYA ,  Tatsuya SHINODA ,  Kazuya TAKIZAWA

IPC分类号： C12N5/079 ,  C12N15/113

CPC分类号： C12N5/0622 ,  C12N15/113 ,  C12N2310/141 ,  C12N2501/727

摘要： A production method for highly proliferating cells or ectodermal progenitor cells, the production method including (i) preparing ectodermal cells which serve as a raw material, (ii) culturing the ectodermal cells which serve as the raw material in a medium containing a small-molecule signaling pathway inhibitor by bringing the inhibitor into contact with the ectodermal cells, and (iii) after the contact, performing additional culturing in a medium containing the inhibitor to obtain a culture containing highly proliferating cells that have higher cell proliferative ability than the ectodermal cells which serve as the raw material; highly proliferating cells having characteristics of ectodermal cells and proliferative ability that allows the number of the highly proliferating cells after more than 28 days of a culturing period during which the cells are brought into contact with a small-molecule signaling pathway inhibitor to be greater than 1.0 times the number of ectodermal cells.

公开(公告)号：US20240352415A1

公开(公告)日：2024-10-24

申请号：US18687436

申请日：2022-08-29

申请人： Albert Einstein College of Medicine

发明人： Wei Liu

IPC分类号： C12N5/079 ,  C12N5/071

CPC分类号： C12N5/0621 ,  C12N5/0697 ,  C12N2506/02 ,  C12N2506/45 ,  C12N2513/00 ,  C12N2533/90

摘要： Provided herein are organoids having concentric zones of telencephalic and ocular progenitors and methods of producing these organoids. Also provided herein are retinal ganglion cells (RGCs) growing directional axons in these organoids and methods of isolating RGCs.

公开(公告)号：US20240279604A1

公开(公告)日：2024-08-22

申请号：US18582703

申请日：2024-02-21

申请人： Industry-University Cooperation Foundation Hanyang University Erica Campus

发明人： Chong Suk RYOU ,  Hyun Jung PARK

IPC分类号： C12N5/0793 ,  C12M1/00 ,  C12N5/00 ,  C12N5/079

CPC分类号： C12N5/0619 ,  C12M23/02 ,  C12N5/0018 ,  C12N5/0622 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2501/115 ,  C12N2503/02 ,  C12N2506/08 ,  C12N2513/00

摘要： The present invention relates to a method for producing a spheroid including neurons and astrocytes, the method including: culturing induced pluripotent stem cell-neural progenitor cells (iPSC-NPCs) in an NPC medium; culturing the cultured cells in a neuronal differentiation medium (NDM); and culturing the cultured cells in a glial differentiation medium (GDM). The spheroid produced by the method according to the present invention contains mature neurons and astrocytes which express a desired marker after 3 weeks of in vitro culture and has a uniform size, and thus can be used for simple visual screening to confirm drug efficacy.

公开(公告)号：US12060583B2

公开(公告)日：2024-08-13

申请号：US17046871

申请日：2019-04-19

申请人： Merck Sharp & Dohme LLC

发明人： Adam Kristopeit ,  Janelle Konietzko ,  Wanli Ma ,  Katherine Phillips ,  Andrew Swartz ,  Sheng-Ching Wang ,  Marc D. Wenger ,  Matthew Woodling ,  Tiago Matos

IPC分类号： B01D15/36 ,  A61K39/12 ,  A61K39/245 ,  C12N5/079 ,  C12N7/02

CPC分类号： C12N7/02 ,  B01D15/362 ,  B01D15/363 ,  C12N5/0621 ,  C12N2710/16111

摘要： The present invention relates to a scalable process for the purification of human cytomegalovirus particles from cell culture medium. In particular, the process involves a two step chromatography process starting with an anion exchange chromatography step followed by a polishing chromatography step selected from mixed mode chromatography or cation exchange chromatography.

公开(公告)号：US12054698B2

公开(公告)日：2024-08-06

申请号：US17670404

申请日：2022-02-11

申请人： The Regents of the University of California

发明人： Momoko Watanabe ,  Kenichiro Kamei

IPC分类号： C12M3/00 ,  C12M1/00 ,  C12M1/12 ,  C12M1/42 ,  C12N5/079 ,  G01N33/50

CPC分类号： C12M21/08 ,  C12M25/00 ,  C12M29/04 ,  C12M35/08 ,  C12N5/0618 ,  G01N33/5032 ,  G01N33/5088 ,  C12N2513/00

摘要： The disclosure provides for multilayered organ-on-a-chip systems that can be used to generate topographic neural organoids, and uses thereof, including as models to study neurological disorders.

公开(公告)号：US20240252544A1

公开(公告)日：2024-08-01

申请号：US18290392

申请日：2022-05-13

申请人： RIKEN ,  Sumitomo Pharma Co., Ltd.

发明人： Michiko Mandai ,  Suguru Yamasaki

IPC分类号： A61K35/30 ,  C12N5/079

CPC分类号： A61K35/30 ,  C12N5/0618 ,  C12N2501/155 ,  C12N2501/727 ,  C12N2506/02 ,  C12N2513/00 ,  C12N2533/52

摘要： An object of the present invention is to provide a method for producing a retinal cell or retinal tissue with a reduced proportion of non-target cells in differentiating pluripotent stem cells. The method of the present invention for producing a retinal cell or retinal tissue comprises: (A) suspension-culturing a pluripotent stem cell to form a cell aggregate of pluripotent stem cells; and (B) suspension-culturing the cell aggregate obtained in the step (A) in the presence of a BMP signaling pathway agonist and a CHK1 signaling pathway inhibitor to obtain a cell aggregate containing a retinal cell.

公开(公告)号：US20240245832A1

公开(公告)日：2024-07-25

申请号：US18551085

申请日：2022-03-18

申请人： THE UNIVERSITY OF SYDNEY ,  SOUTH EASTERN SYDNEY LOCAL HEALTH DISTRICT

发明人： Jingjing YOU ,  Gerard SUTTON ,  Morgan OVERMASS ,  Constantinos PETSOGLOU ,  Sheng HUA ,  Li WEN

IPC分类号： A61L27/38 ,  A61L27/24 ,  A61L27/36 ,  A61L27/54 ,  C12N5/079

CPC分类号： A61L27/3808 ,  A61L27/24 ,  A61L27/3616 ,  A61L27/3687 ,  A61L27/54 ,  C12N5/0621 ,  A61L2300/414 ,  A61L2430/16 ,  C12N2500/32 ,  C12N2500/38 ,  C12N2501/165 ,  C12N2533/54 ,  C12N2533/56 ,  C12N2537/10

摘要： The present invention relates to bioengineered collagen constructs and methods for the preparation and use of said constructs. The constructs and methods may find application in, but are not limited to, the provision of replacement tissue and/or cells, and/or the delivery of agents to biological targets such as tissues and cells. The constructs and methods may also find application in, but are not limited to, replacing endothelial tissue.

公开(公告)号：US12042576B2

公开(公告)日：2024-07-23

申请号：US18116472

申请日：2023-03-02

申请人： NATIONAL DEFENSE MEDICAL CENTER

发明人： Chung-Hsing Chou ,  Dueng-Yuan Hueng ,  Tsung-Neng Tsai

IPC分类号： A61L27/38 ,  A61K35/30 ,  A61K35/44 ,  A61L27/24 ,  A61L27/36 ,  C12N5/071 ,  C12N5/079

CPC分类号： A61L27/3891 ,  A61K35/30 ,  A61K35/44 ,  A61L27/24 ,  A61L27/3633 ,  A61L27/3675 ,  A61L27/3808 ,  A61L27/383 ,  A61L27/3878 ,  C12N5/0618 ,  C12N5/0697 ,  A61L2430/32 ,  C12N2500/25 ,  C12N2500/46 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/392 ,  C12N2502/088 ,  C12N2502/28 ,  C12N2533/54 ,  A61K35/30 ,  A61K2300/00 ,  A61K35/44 ,  A61K2300/00

摘要： The invention relates to a cell sheet construct for neurovascular reconstruction. The cell sheet construct has a vascular endothelial cell layer and a neural stem cell layer, and the two layers are physically in direct contact with each other, where the vascular endothelial cell layer forms branching vasculatures, and the neural stem cell layer differentiates into neurons. The invention also relates to a method for manufacturing the cell sheet construct, having the following steps: culturing vascular endothelial cells on a substrate to form a vascular endothelial cell layer, seeding neural stem cells on the vascular endothelial cell layer to make the neural stem cells be physically in direct contact with the vascular endothelial cell layer, and culturing the neural stem cells and the vascular endothelial cell layer to differentiate into neurons and branching vasculatures to form a cell sheet construct.

公开(公告)号：US20240228955A9

公开(公告)日：2024-07-11

申请号：US18453712

申请日：2023-08-22

申请人： BioLamina AB

发明人： Outi Hovatta ,  Karl Tryggvason

IPC分类号： C12N5/079 ,  A61K9/00 ,  A61K35/30 ,  C12N5/00

CPC分类号： C12N5/0621 ,  A61K9/0019 ,  A61K9/0048 ,  A61K35/30 ,  C12N5/0056 ,  C12N2500/92 ,  C12N2500/98 ,  C12N2501/115 ,  C12N2506/02 ,  C12N2533/50 ,  C12N2533/52

摘要： The present disclosure relates to the use of laminin-521 in obtaining retinal pigment epithelium (RPE) cells. Pluripotent human embryonic stem cells are cultured on plates coated with recombinant laminin-521 (laminin-11), in totally defined and xeno-free conditions. A first cell culture medium contains a growth factor, and a second cell culture medium does not contain growth factor. The stem cells are first exposed to the first cell culture medium, then exposed to the second cell culture medium for a longer time period. After a number of weeks, clinical grade RPE cells are obtained from the stem cells.

公开(公告)号：US20240228954A1

公开(公告)日：2024-07-11

申请号：US18529198

申请日：2023-12-05

申请人： EMULATE, Inc. ,  Cedars-Sinai Medical Center

发明人： S. Jordan Kerns ,  Norman Wen ,  Carolina Lucchesi ,  Christopher David Hinojosa ,  Jacob Fraser ,  Geraldine Hamilton ,  Gad Vatine ,  Samuel Sances ,  Clive Svendsen ,  Daniel Levner ,  Dhruv Sareen

IPC分类号： C12N5/0793 ,  C12N5/071 ,  C12N5/079 ,  C12Q1/02 ,  B01L3/00 ,  C12M1/12 ,  C12M1/42 ,  C12M3/06

CPC分类号： C12N5/0619 ,  C12N5/0622 ,  C12N5/069 ,  C12N5/0692 ,  C12Q1/02 ,  B01L3/5027 ,  B01L2200/0647 ,  B01L2300/0861 ,  C12M23/16 ,  C12M25/02 ,  C12M35/08 ,  C12N5/0618 ,  C12N2502/081 ,  C12N2502/086 ,  C12N2502/28 ,  C12N2506/45 ,  C12N2531/00 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/56 ,  C12N2535/10 ,  C12N2539/00

摘要： The invention relates to culturing brain endothelial cells, and optionally astrocytes and neurons in a fluidic device under conditions whereby the cells mimic the structure and function of the blood brain barrier. Culture of such cells in a microfluidic device, whether alone or in combination with other cells, drives maturation and/or differentiation further than existing systems.