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公开(公告)号：US12070746B2
公开(公告)日：2024-08-27
申请号：US15958142
申请日：2018-04-20
发明人： Dan Gazit , Gadi Pelled , Zulma Gazit , Dmitriy Sheyn , Christopher David Hinojosa , Norman Wen , Geraldine Hamilton
IPC分类号： C12N5/077 , A61K49/00 , A61K51/02 , A61L27/38 , B01L3/00 , C12N5/00 , C12N5/071 , C12N5/074 , C12N5/0775 , G01N33/50 , G01T1/164 , G01T1/29 , C07K14/78 , C12M1/34
CPC分类号： B01L3/5027 , A61K49/0019 , A61K49/005 , A61K51/02 , A61L27/3895 , B01L3/5085 , C12N5/0062 , C12N5/0075 , C12N5/0654 , C12N5/0655 , C12N5/0662 , C12N5/0663 , C12N5/0668 , C12N5/0696 , C12N5/0697 , G01N33/5038 , G01T1/1644 , G01T1/2985 , A61M2205/3334 , C07K14/78 , C12M41/40
摘要： Microfluidic “organ-on-a-chip” devices have been developed with the aim to replicate human tissues in vitro. However, there is no option to quantitatively monitor biological processes that take place within the chip, over time. Destructive methods in order to analyze, tissue formation, gene expression, protein secretion etc. require the harvest of the “tissue” at a certain time point. Described herein are methods and compositions for non-destructive molecular imaging methods and systems in order to quantitatively monitor specific biological processes, over time, within the chip, without the need to harvest.
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公开(公告)号：US11174462B2
公开(公告)日：2021-11-16
申请号：US15955335
申请日：2018-04-17
发明人： S. Jordan Kerns , Norman Wen , Carolina Lucchesi , Christopher David Hinojosa , Jacob Fraser , Geraldine Hamilton , Gad Vatine , Samuel Sanees , Clive Svendsen , Daniel Levner , Dhruv Sareen
摘要： The invention relates to culturing brain endothelial cells, and optionally astrocytes and neurons in a fluidic device under conditions whereby the cells mimic the structure and function of the blood brain barrier. Culture of such cells in a microfluidic device, whether alone or in combination with other cells, drives maturation and/or differentiation further than existing systems.
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公开(公告)号：US20180305651A1
公开(公告)日：2018-10-25
申请号：US15768736
申请日：2016-10-19
发明人： S. Jordan Kerns , Norman Wen , Carolina Lucchesi , Christopher David Hinojosa , Jacob Fraser , Geraldine Hamilton , Gad Vatine , Samuel Sances , Clive Svendsen , Daniel Levner , Dhruv Sareen
IPC分类号： C12M3/06 , C12N5/0793 , C12N5/071 , B01L3/00
CPC分类号： C12N5/0619 , B01L3/5027 , B01L2200/0647 , B01L2300/0861 , C12M23/16 , C12M25/02 , C12M35/08 , C12N5/0618 , C12N5/0622 , C12N5/069 , C12N5/0692 , C12N2502/081 , C12N2502/086 , C12N2502/28 , C12N2506/45 , C12N2531/00 , C12N2533/52 , C12N2533/54 , C12N2533/56 , C12N2535/10 , C12N2539/00 , C12Q1/02
摘要： The invention relates to culturing brain endothelial cells, and optionally astrocytes and neurons in a fluidic device under conditions whereby the cells mimic the structure and function of the blood brain barrier. Culture of such cells in a microfluidic device, whether alone or in combination with other cells, drives maturation and/or differentiation further than existing systems.
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公开(公告)号：US20180298332A1
公开(公告)日：2018-10-18
申请号：US15955383
申请日：2018-04-17
发明人： S. Jordan Kerns , Norman Wen , Carolina Lucchesi , Christopher David Hinojosa , Jacob Fraser , Geraldine Hamilton , Gad Vatine , Samuel Sances , Clive Svendsen , Daniel Levner , Dhruv Sareen
IPC分类号： C12N5/0793 , C12N5/071 , C12M3/06 , C12M1/12 , C12M1/42
摘要： The invention relates to culturing brain endothelial cells, and optionally astrocytes and neurons in a fluidic device under conditions whereby the cells mimic the structure and function of the blood brain barrier. Culture of such cells in a microfluidic device, whether alone or in combination with other cells, drives maturation and/or differentiation further than existing systems.
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公开(公告)号：US12091650B2
公开(公告)日：2024-09-17
申请号：US16286185
申请日：2019-02-26
发明人： S. Jordan Kerns , Norman Wen , Carolina Lucchesi , Christopher David Hinojosa , Jacob Fraser , Geraldine Hamilton , Gad Vatine , Samuel Sances , Clive Svendsen , Daniel Levner , Dhruv Sareen
CPC分类号： C12M23/16 , B01L3/502715 , C12M25/02 , C12N5/0068 , C12N5/0619 , C12N5/0622 , C12N5/069 , B01L2200/0647 , B01L2300/0861 , C12N2502/081 , C12N2502/28 , C12N2506/02 , C12N2506/45 , C12N2531/00 , C12N2539/00
摘要： The invention relates to culturing brain endothelial cells, and optionally astrocytes and neurons in a fluidic device under conditions whereby the cells mimic the structure and function of the blood brain barrier. Culture of such cells in a microfluidic device, whether alone or in combination with other cells, drives maturation and/or differentiation further than existing systems.
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公开(公告)号：US20220282221A1
公开(公告)日：2022-09-08
申请号：US17678485
申请日：2022-02-23
发明人： S. Jordan Kerns , Norman Wen , Carol Lucchesi , Christopher David Hinojosa , Jacob Fraser , Jefferson Puerta , Geraldine Hamilton , Robert Barrett , Clive Svendsen , Daniel Levner , Stephen R. Targan , Michael Workman , Dhruv Sareen , Uthra Rajamani , Magdalena Kasendra
摘要： Organs-on-chips are microfluidic devices for culturing living cells in micrometer sized chambers in order to model physiological functions of tissues and organs. Engineered patterning and continuous fluid flow in these devices has allowed culturing of intestinal cells bearing physiologically relevant features and sustained exposure to bacteria while maintaining cellular viability, thereby allowing study of inflammatory bowl diseases. However, existing intestinal cells do not possess all physiologically relevant subtypes, do not possess the repertoire of genetic variations, or allow for study of other important cellular actors such as immune cells. Use of iPSC-derived epithelium, including IBD patient-specific cells, allows for superior disease modeling by capturing the multi-faceted nature of the disease.
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公开(公告)号：US20180305668A1
公开(公告)日：2018-10-25
申请号：US15958142
申请日：2018-04-20
发明人： Dan Gazit , Gadi Pelled , Zulma Gazit , Dmitriy Sheyn , Christopher David Hinojosa , Norman Wen , Geraldine Hamilton
IPC分类号： C12N5/077 , C12N5/0775 , C12N5/00
CPC分类号： C12N5/0075 , A61K49/0019 , A61K49/005 , B01L3/5027 , C07K14/78 , C12N5/0062 , C12N5/0654 , C12N5/0663 , G01N33/5038
摘要： Microfluidic “organ-on-a-chip” devices have been developed with the aim to replicate human tissues in vitro. However, there is no option to quantitatively monitor biological processes that take place within the chip, over time. Destructive methods in order to analyze, tissue formation, gene expression, protein secretion etc. require the harvest of the “tissue” at a certain time point. Described herein are methods and compositions for non-destructive molecular imaging methods and systems in order to quantitatively monitor specific biological processes, over time, within the chip, without the need to harvest.
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公开(公告)号：US20170226478A1
公开(公告)日：2017-08-10
申请号：US15458185
申请日：2017-03-14
发明人： Jordan Kerns , Norman Wen , Geraldine Hamilton , Christopher Hinojosa , Jacob Fraser , Catherine Karalis , Janna Nawroth , Dhruv Sareen , Anjoscha Kaus , Berhan Mandefro , Hyoung Shin Park , Ville Kujala
IPC分类号： C12N5/0793 , B01L3/00 , C12N5/077
CPC分类号： C12N5/0619 , C12M23/16 , C12N5/0658 , C12N2502/083 , C12N2506/45 , C12N2531/00 , C12N2533/52
摘要： The invention relates to culturing motor neuron cells together with skeletal muscle cells in a fluidic device under conditions whereby the interaction of these cells mimic the structure and function of the neuromuscular junction (NMJ) providing a NMJ-on-chip. Good viability, formation of myo-fibers and function of skeletal muscle cells on fluidic chips allow for measurements of muscle cell contractions. Embodiments of motor neurons co-cultures with contractile myo-fibers are contemplated for use with modeling diseases affecting NMJ's, e.g. Amyotrophic lateral sclerosis (ALS).
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公开(公告)号：US20240228954A1
公开(公告)日：2024-07-11
申请号：US18529198
申请日：2023-12-05
发明人： S. Jordan Kerns , Norman Wen , Carolina Lucchesi , Christopher David Hinojosa , Jacob Fraser , Geraldine Hamilton , Gad Vatine , Samuel Sances , Clive Svendsen , Daniel Levner , Dhruv Sareen
CPC分类号： C12N5/0619 , C12N5/0622 , C12N5/069 , C12N5/0692 , C12Q1/02 , B01L3/5027 , B01L2200/0647 , B01L2300/0861 , C12M23/16 , C12M25/02 , C12M35/08 , C12N5/0618 , C12N2502/081 , C12N2502/086 , C12N2502/28 , C12N2506/45 , C12N2531/00 , C12N2533/52 , C12N2533/54 , C12N2533/56 , C12N2535/10 , C12N2539/00
摘要： The invention relates to culturing brain endothelial cells, and optionally astrocytes and neurons in a fluidic device under conditions whereby the cells mimic the structure and function of the blood brain barrier. Culture of such cells in a microfluidic device, whether alone or in combination with other cells, drives maturation and/or differentiation further than existing systems.
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公开(公告)号：US11952592B2
公开(公告)日：2024-04-09
申请号：US17678485
申请日：2022-02-23
发明人： S. Jordan Kerns , Norman Wen , Carol Lucchesi , Christopher David Hinojosa , Jacob Fraser , Jefferson Puerta , Geraldine Hamilton , Robert Barrett , Clive Svendsen , Daniel Levner , Stephen R Targan , Michael Workman , Dhruv Sareen , Uthra Rajamani , Magdalena Kasendra
CPC分类号： C12N5/0679 , C12M21/08 , C12M23/16 , C12N5/0618 , C12N5/0696 , G01N33/5005 , C12N2501/11 , C12N2501/119 , C12N2501/13 , C12N2501/155 , C12N2501/16 , C12N2501/24 , C12N2501/25 , C12N2501/415 , C12N2501/998 , C12N2501/999 , C12N2506/45 , C12N2535/00
摘要： Organs-on-chips are microfluidic devices for culturing living cells in micrometer sized chambers in order to model physiological functions of tissues and organs. Engineered patterning and continuous fluid flow in these devices has allowed culturing of intestinal cells bearing physiologically relevant features and sustained exposure to bacteria while maintaining cellular viability, thereby allowing study of inflammatory bowl diseases. However, existing intestinal cells do not possess all physiologically relevant subtypes, do not possess the repertoire of genetic variations, or allow for study of other important cellular actors such as immune cells. Use of iPSC-derived epithelium, including IBD patient-specific cells, allows for superior disease modeling by capturing the multi-faceted nature of the disease.
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