公开(公告)号：US20240352426A1

公开(公告)日：2024-10-24

申请号：US18686708

申请日：2022-08-25

Applicant: Vanderbilt University ,  The United States as Represented by The Department of Veterans Affairs

Inventor： Lauren E. WOODARD ,  Julie BEJOY

IPC: C12N5/071 ,  A61K35/545

CPC classification number: C12N5/0686 ,  A61K35/545 ,  C12N2500/38 ,  C12N2501/119 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/165 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2501/91 ,  C12N2506/45 ,  C12N2533/52

Abstract: Disclosed herein is a method for producing intermediate mesodermal cells from human pluripotent stem cells. Also disclosed is a method for producing nephron progenitor cells from human pluripotent stem cells. Also disclosed is a method for producing podocytes. Also disclosed is a method for treating a kidney disease in a subject, that involves administering to the subject a therapeutically effective amount of the produced intermediate mesodermal cells, nephron progenitor cells, and/or podocytes.

公开(公告)号：US20240352413A1

公开(公告)日：2024-10-24

申请号：US18639238

申请日：2024-04-18

Applicant: THE GENERAL HOSPITAL CORPORATION

Inventor： Jayaraj RAJAGOPAL ,  Hongmei Mou

IPC: C12N5/074 ,  A61K9/00 ,  A61K35/42 ,  A61L27/38 ,  A61L27/56 ,  A61L27/58 ,  C12N5/071 ,  G01N33/68 ,  G03B21/28

CPC classification number: C12N5/0607 ,  A61K35/42 ,  A61L27/3834 ,  A61L27/3882 ,  A61L27/56 ,  A61L27/58 ,  C12N5/0678 ,  C12N5/068 ,  C12N5/0688 ,  C12N5/0689 ,  G01N33/6884 ,  G03B21/28 ,  A61K9/0073 ,  A61L2430/22 ,  C12N2501/115 ,  C12N2501/117 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/385 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2506/02 ,  C12N2506/45 ,  C12N2533/90

Abstract: Provided herein are methods and compositions relating, in part, to the generation of human progenitor cells committed to the lung lineage and uses of such cells for treatment of lung diseases/disorders or injury to the lung. Whether an adult stem cell can be isolated from human adult lung remains controversial in the art and at present, methods for isolating and using adult lung stem cells from humans lack reproducibility. Thus, the methods and compositions described herein are advantageous over the present state of knowledge in the art and permit the generation of human lung progenitor cells for treatment, tissue engineering, and screening assays.

公开(公告)号：US20240344034A1

公开(公告)日：2024-10-17

申请号：US18757662

申请日：2024-06-28

Applicant: The University of Queensland

Inventor： Melissa Little ,  Minoru Takasato

IPC: C12N5/071 ,  G01N33/50

CPC classification number: C12N5/0687 ,  G01N33/5014 ,  G01N33/5044 ,  C12N2501/119 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/385 ,  C12N2501/415 ,  C12N2501/91 ,  C12N2506/02 ,  C12N2506/45

Abstract: A method is provided for simultaneously producing both nephron progenitor cells and ureteric epithelial progenitor cells including the step of contacting intermediate mesoderm cells with: fibroblast growth factor 9 and/or fibroblast growth factor 20 and optionally, one or more selected from the group consisting of: bone morphogenic protein 7; heparin; a Wnt agonist; retinoic acid; and an RA antagonist. The concentrations of Wnt agonist, retinoic acid and/or RA antagonist may be manipulated to favour the relative production of nephron progenitor cells and ureteric epithelial progenitor cells. The intermediate mesoderm cells are ultimately derived from human pluripotent stem cells via a posterior primitive streak stage. The nephron progenitor cells and ureteric epithelial progenitor cells may have end uses such as for kidney repair and regeneration, bioprinting of kidneys and screening compounds for nephrotoxicity.

公开(公告)号：US20240342218A1

公开(公告)日：2024-10-17

申请号：US18294700

申请日：2022-08-03

Applicant: University Health Network

Inventor： Michael George FEHLINGS ,  Mohammad KHAZAEI ,  Christopher S. AHUJA

IPC: A61K35/30 ,  A61P25/00 ,  C12N5/0797

CPC classification number: A61K35/30 ,  A61P25/00 ,  C12N5/0623 ,  C12N2501/119 ,  C12N2501/13 ,  C12N2501/155 ,  C12N2501/385 ,  C12N2506/45

Abstract: The present disclosure relates generally to neural progenitor cells and therapeutic uses thereof. More particularly, the present disclosure provides cervical spinal cord-specific neural progenitor cells (cerNPCs), methods of producing cerNPCs, pharmaceutical compositions comprising cerNPCs. and methods of treating neurological diseases or disorders with the cerNPCs.

公开(公告)号：US20240318146A1

公开(公告)日：2024-09-26

申请号：US18684587

申请日：2022-08-17

Applicant: CHILDREN’S HOSPITAL MEDICAL CENTER

Inventor： Mingxia GU ,  Yifei MIAO

IPC: C12N5/071

CPC classification number: C12N5/0697 ,  C12N5/0679 ,  C12N5/0688 ,  C12N5/0691 ,  C12N2500/84 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/415 ,  C12N2506/45 ,  C12N2513/00

Abstract: Disclosed herein are compositions of organoids comprising a mesodermal vascular network and definitive endoderm derivatives. Also disclosed herein are methods making use of human pluripotent stem cells to differentiate cells of multiple germ layer lineages with proper organization. These organoids exhibit vasculature and can be used as models for studying endoderm-derived organogenesis and vascular interactions.

公开(公告)号：US20240318144A1

公开(公告)日：2024-09-26

申请号：US18433553

申请日：2024-02-06

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Yaqub HANNA ,  Noa NOVERSHTERN ,  Yoach RAIS

IPC: C12N5/074 ,  C12N5/00 ,  C12N5/0735

CPC classification number: C12N5/0696 ,  C12N5/0037 ,  C12N5/0606 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2501/01 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/235 ,  C12N2501/415 ,  C12N2501/50 ,  C12N2501/60 ,  C12N2501/71 ,  C12N2501/72 ,  C12N2501/727 ,  C12N2501/73 ,  C12N2501/999 ,  C12N2506/00

Abstract: A culture medium is disclosed which comprises STAT3 activator, an ERK1/2 inhibitor and an Axin stabilizer, and optionally also a PKC inhibitor. Cell cultures comprising same and uses thereof are also disclosed.

公开(公告)号：US12098352B2

公开(公告)日：2024-09-24

申请号：US16983850

申请日：2020-08-03

Applicant: EMULATE, INC.

Inventor： Janna Nawroth ,  Riccardo Barrile ,  David Conegliano ,  Remi Villenave ,  Carolina Lucchesi ,  Justin Nguyen ,  Antonio Varone ,  Catherine Karalis ,  Geraldine Hamilton

IPC: C12M3/06 ,  B01L3/00 ,  C12N5/071 ,  C12N5/074

CPC classification number: C12M23/16 ,  B01L3/5027 ,  C12N5/0688 ,  C12N5/0696 ,  C12N2501/115 ,  C12N2501/117 ,  C12N2501/119 ,  C12N2501/155 ,  C12N2501/41 ,  C12N2502/1323 ,  C12N2502/27 ,  C12N2503/04 ,  C12N2506/02 ,  C12N2513/00 ,  G01N2800/12

Abstract: An in vitro microfluidic “organ-on-chip” device is described herein that mimics the structure and at least one function of specific areas of the epithelial system in vivo. In particular, a stem cell-based Lung-on-Chip is described. This in vitro microfluidic system can be used for modeling differentiation of cells on-chip into lung cells, e.g., a lung (Lung-On-Chip), bronchial (Airway-On-Chip; small-Airway-On-Chip), alveolar sac (Alveolar-On-Chip), etc., for use in modeling disease states of derived tissue, i.e. as healthy, pre-disease and diseased tissues. Additionally, stem cells under differentiation protocols for deriving (producing) differentiated lung cells off-chips may be seeded onto microfluidic devices at any desired point during the in vitro differentiation pathway for further differentiation on-chip or placed on-chip before, during or after terminal differentiation.

公开(公告)号：US20240294501A1

公开(公告)日：2024-09-05

申请号：US18581700

申请日：2024-02-20

Applicant: Vertex Pharmaceuticals Incorporated

Inventor： Austin Thiel ,  Jihad Yasin ,  Evrett Thompson ,  Felicia J. Pagliuca

IPC: C07D401/14 ,  C07D401/04 ,  C07K14/495 ,  C07K14/50 ,  C12N5/071

CPC classification number: C07D401/14 ,  C07D401/04 ,  C07K14/495 ,  C07K14/50 ,  C12N5/0676 ,  C12N2501/117 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/385 ,  C12N2501/41

Abstract: Disclosed herein are compositions and methods useful for manufacturing SC-β cell, and isolated populations of SC-β cells for use in various applications, such as cell therapy.

公开(公告)号：US12077778B2

公开(公告)日：2024-09-03

申请号：US16989554

申请日：2020-08-10

Applicant: INDIANA UNIVERSITY RESEARCH AND TECHNOLOGY CORPORATION

Inventor： Karl R. Koehler ,  Eri Hashino

IPC: C12N5/0793 ,  C12N5/0735 ,  C12N5/074

CPC classification number: C12N5/062 ,  C12N5/0606 ,  C12N5/0607 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/415 ,  C12N2506/02 ,  C12N2533/52 ,  C12N2533/90

Abstract: Provided herein are methods for directing differentiation of human pluripotent stem cells into inner ear sensory epithelia and sensory neurons. More particularly, provided herein are methods for obtaining three-dimensional cultures comprising human pluripotent stem cell-derived pre-otic epithelium, otic vesicles, and inner ear sensory epithelia containing hair cells, sensory neurons, and supporting cells.

公开(公告)号：US20240277774A1

公开(公告)日：2024-08-22

申请号：US18623641

申请日：2024-04-01

Applicant: BONUS THERAPEUTICS LTD.

Inventor： Shai MERETZKI ,  Dror BEN-DAVID ,  Atara NOVIK ,  Ronit SHTRICHMAN

IPC: A61K35/28 ,  A61K35/00 ,  A61K35/12 ,  A61P19/00 ,  C12N5/00 ,  C12N5/077 ,  C12Q1/68 ,  C12Q1/6876

CPC classification number: A61K35/28 ,  A61K35/12 ,  A61P19/00 ,  C12N5/0062 ,  C12N5/0653 ,  C12Q1/68 ,  C12Q1/6876 ,  A61K35/00 ,  C12N5/0075 ,  C12N5/0652 ,  C12N2501/155 ,  C12Q2600/158

Abstract: A composition comprising a cell population wherein the population is suitable for transplantation into a subject in need thereof, and characterized by differences of expression levels of a plurality of genes. Further, methods and kits for identifying a cell population suitable for transplantation into a subject in need thereof.