公开(公告)号：US12129492B2

公开(公告)日：2024-10-29

申请号：US17343411

申请日：2021-06-09

申请人： D. Lansing Taylor ,  Albert Gough ,  Larry Vernetti

发明人： D. Lansing Taylor ,  Albert Gough ,  Larry Vernetti

IPC分类号： C12N5/071 ,  B01L3/00 ,  C12M1/42 ,  C12M3/06 ,  G01N33/50

CPC分类号： C12N5/0697 ,  B01L3/502715 ,  B01L3/502761 ,  C12M23/16 ,  C12M35/08 ,  C12N5/067 ,  G01N33/5067 ,  G01N33/5082 ,  B01L2300/0636 ,  C12N2502/11 ,  C12N2502/14 ,  C12N2502/28 ,  C12N2510/00 ,  C12N2513/00 ,  C12N2533/50 ,  C12N2533/54

摘要： Microfluidic devices for modeling three-dimensional tissue structures and methods for making and using the same are described herein.

公开(公告)号：US20240352419A1

公开(公告)日：2024-10-24

申请号：US18025588

申请日：2022-12-02

申请人： NUWACELL BIOTECHNOLOGIES CO., LTD.

发明人： Junying YU ,  Tao ZHOU ,  Changqing TIAN ,  Yali GAO ,  Ying ZHANG

IPC分类号： C12N5/0783 ,  C12N5/071 ,  C12N5/0789

CPC分类号： C12N5/0646 ,  C12N5/0647 ,  C12N5/069 ,  C12N2500/30 ,  C12N2500/90 ,  C12N2501/165 ,  C12N2501/91 ,  C12N2506/45 ,  C12N2513/00

摘要： The present disclosure provides, inter alia, methods and compositions for differentiation of pluripotent stem cells and derived hematopoietic lineage cells including hemogenic endothelial cells, hematopoietic progenitor cells and natural killer cells. The differentiation efficiency for the hemogenic endothelial cells, the hematopoietic progenitor cells and the natural killer cells can be improved by using the methods and compositions of this disclosure described herein.

公开(公告)号：US20240344033A1

公开(公告)日：2024-10-17

申请号：US18682573

申请日：2021-08-18

申请人： TOHOKU UNIVERSITY

发明人： Takahiro ARIMA ,  Shun SHIBATA ,  Hiroaki OKAE

IPC分类号： C12N5/071 ,  G09B23/30

CPC分类号： C12N5/0682 ,  G09B23/306 ,  C12N2502/02 ,  C12N2503/04 ,  C12N2513/00 ,  C12N2533/54

摘要： There is provided an endometrial model including: a stromal extracellular matrix-containing gel; and endometrial epithelial cells, wherein at least some of the endometrial epithelial cells are exposed on a surface of the gel, and wherein the at least some of the endometrial epithelial cells exposed on the surface of the gel continuously form a luminal structure and a non-luminal structure. Also, a method for preparing the endometrial model is also provided. Further, an endometrial implantation model including: the endometrial model and an embryonic cell is provided.

公开(公告)号：US12110510B2

公开(公告)日：2024-10-08

申请号：US17253038

申请日：2019-06-20

申请人： The Board of Trustees of the Leland Stanford Junior University

发明人： Jimena Andersen ,  Sergiu P. Pasca

IPC分类号： C12N5/071 ,  C12N5/077 ,  C12N5/0793 ,  G01N33/50

CPC分类号： C12N5/0697 ,  C12N5/0619 ,  C12N5/0658 ,  G01N33/5082 ,  C12N2500/38 ,  C12N2501/01 ,  C12N2501/105 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/13 ,  C12N2501/15 ,  C12N2501/41 ,  C12N2501/734 ,  C12N2501/999 ,  C12N2502/081 ,  C12N2502/1335 ,  C12N2506/45 ,  C12N2513/00

摘要： Functional human cortico-spinal-muscle assembled spheroids are generated by in vitro culture. Complete cortico-spinal-muscle spheroids (hCS-hSC-hSkM) are assembled from component cultured cell systems, where each cultured cell system is designed to provide specific sets of neural and/or muscle cells, and which components are functionally integrated in the assembled spheroid.

公开(公告)号：US20240327800A1

公开(公告)日：2024-10-03

申请号：US18625271

申请日：2024-04-03

申请人： University of Massachusetts

发明人： Feiyu Yang ,  Narciso Pavon ,  ChangHui Pak ,  Yubing Sun

IPC分类号： C12N5/071 ,  C12M1/32 ,  C12N5/00 ,  C12N5/079

CPC分类号： C12N5/0697 ,  C12M23/12 ,  C12N5/0062 ,  C12N5/0618 ,  C12N2501/41 ,  C12N2506/00 ,  C12N2513/00 ,  C12N2533/54

摘要： The present disclosure provides a device and methods for patterning organoids. The device includes a plurality of layers vertically arranged. The plurality of layers includes a base layer, a diffusion medium disposed on the base layer, a microwell layer configured above the diffusion medium, and a partitioning layer. The microwell layer includes a lower surface, an upper surface, and one or more microwells. Each microwell defines an opening configured to extend between the microwell and the diffusion medium. The partitioning layer defines a chemical reservoir and one or more medium chambers, where the wall divides the chemical reservoir from the one or more medium chambers.

公开(公告)号：US20240327770A1

公开(公告)日：2024-10-03

申请号：US18675517

申请日：2024-05-28

申请人： TOYO SEIKAN GROUP HOLDINGS, LTD.

发明人： Azusa OTSUKI ,  Masahiro KUNINORI ,  Kentarou ICHIKAWA

IPC分类号： C12M1/12 ,  C08L1/02 ,  C12N5/00

CPC分类号： C12M25/02 ,  C08L1/02 ,  C12N5/0062 ,  C12N2513/00

摘要： The disclosure makes it possible to provide a cell culture membrane capable of easily decomposing after allowing cell layers to be formed on both sides of the cell culture membrane. A cell culture membrane having a cell culture region at both surfaces thereof, the cell culture membrane containing methyl cellulose having a weight average molecular weight of from 20000 to 190000 as a main component, or containing methyl cellulose having a weight average molecular weight of from 190000 to 400000 as a main component, and further containing a dissolution temperature regulator. The dissolution temperature regulator is preferably at least one of sodium styrenesulfonate, sodium iodide, or sodium toluenesulfonate. A poorly soluble material is preferably added to the cell culture membrane.

公开(公告)号：US20240318145A1

公开(公告)日：2024-09-26

申请号：US18573954

申请日：2022-06-30

申请人： Children's Hospital Medical Center

发明人： James Macormack Wells ,  Alexandra Kay Eicher

IPC分类号： C12N5/071

CPC分类号： C12N5/0697 ,  C12N2500/38 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/155 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2503/00 ,  C12N2506/45 ,  C12N2513/00

摘要： Disclosed herein are compositions of gastrointestinal organoids comprising cells originating from all three primary germ layers and methods of making and use thereof. These gastrointestinal organoids exhibit complex cellular organization and functions resembling naturally occurring organ tissue, and serve as excellent three dimensional models for studying gastrointestinal physiology.

公开(公告)号：US20240301339A1

公开(公告)日：2024-09-12

申请号：US18266951

申请日：2021-12-06

申请人： MITSUI CHEMICALS, INC.

发明人： Katsuhiro ESASHIKA ,  Hiroshi MIYASAKO ,  Takashi KOZEKI ,  Koh AMANO ,  Yoshinobu SAIJO ,  Hisaaki YANAI

IPC分类号： C12M1/12 ,  C12N5/00

CPC分类号： C12M25/14 ,  C12N5/0068 ,  C12N2513/00

摘要： Provided is a culture material and a culture tool, which allow to culture, cells, etc. to form a spheroid.
A culture material for culturing cells, a tissue, or an organ on a culture surface thereof, the culture material containing a 4-methyl-1-pentene polymer (X), having a water contact angle of the culture surface of more than 100° and 160° or less, and having an oxygen permeability at a temperature of 23° C. and humidity of 0% of 4500 to 90000 cm3/(m2×24 h×atm).

公开(公告)号：US12084641B2

公开(公告)日：2024-09-10

申请号：US17395452

申请日：2021-08-05

申请人： Avant Meats Company Limited

发明人： Po San Mario Chin ,  Kai Yi Carrie Chan ,  Chuen Wai Li ,  Tim Spitters

IPC分类号： C12M1/00 ,  A23L13/00 ,  A23L17/00 ,  C12M3/00 ,  C12N5/071 ,  C12N5/074 ,  C12N5/077 ,  C12N5/0775 ,  C12N15/113

CPC分类号： C12M21/08 ,  A23L13/00 ,  A23L17/00 ,  C12M29/04 ,  C12N5/0652 ,  C12N5/0656 ,  C12N5/0662 ,  C12N5/0679 ,  C12N5/0696 ,  C12N5/0697 ,  C12N15/113 ,  A23V2002/00 ,  C12N2310/141 ,  C12N2501/105 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/2306 ,  C12N2501/2311 ,  C12N2501/33 ,  C12N2501/998 ,  C12N2510/00 ,  C12N2513/00 ,  C12N2531/00 ,  C12N2533/74

摘要： A cell/tissue culture system comprising at least one bioreactor configured to hold at least one type of cell to cultivate tissue, a dialysis unit comprising a dialysis membrane, a fresh medium unit, and a waste removal unit configured to remove metabolic waste from dialysate, wherein the metabolic waste comprises ammonia and lactate, and wherein the waste removal unit comprises biocatalysts or enzymes configured to breakdown lactate and generate carbon sources that promote cell growth.

公开(公告)号：US20240294877A1

公开(公告)日：2024-09-05

申请号：US18573536

申请日：2022-06-28

申请人： SAGA UNIVERSITY

发明人： Koichi NAKAYAMA ,  Anna Maria NAKAMURA

IPC分类号： C12N5/077

CPC分类号： C12N5/0655 ,  C12N2501/135 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2506/45 ,  C12N2513/00

摘要： A method for producing a three-dimensional structure of cartilage cells, the method being characterized by comprising layering cell aggregates that have been produced by culturing a mesenchymal stem cell using a culture medium for inducing the differentiation into a cartilage cell on the 9th or 10th day after the starting of the culture.