公开(公告)号：US20240294877A1

公开(公告)日：2024-09-05

申请号：US18573536

申请日：2022-06-28

申请人： SAGA UNIVERSITY

发明人： Koichi NAKAYAMA ,  Anna Maria NAKAMURA

IPC分类号： C12N5/077

CPC分类号： C12N5/0655 ,  C12N2501/135 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2506/45 ,  C12N2513/00

摘要： A method for producing a three-dimensional structure of cartilage cells, the method being characterized by comprising layering cell aggregates that have been produced by culturing a mesenchymal stem cell using a culture medium for inducing the differentiation into a cartilage cell on the 9th or 10th day after the starting of the culture.

公开(公告)号：US20160022870A1

公开(公告)日：2016-01-28

申请号：US14776101

申请日：2013-10-24

申请人： SAGA UNIVERSITY ,  CYFUSE BIOMEDICAL K.K.

发明人： Ryo NOGUCHI ,  Tadashi TAMURA ,  Koichi NAKAYAMA ,  Shigeki MORITA ,  Koichi NODE

IPC分类号： A61L27/36 ,  A61K35/33 ,  A61K35/44 ,  A61K35/34

CPC分类号： A61L27/3625 ,  A61K35/33 ,  A61K35/34 ,  A61K35/44 ,  A61L27/3804 ,  A61L27/3886 ,  A61L27/507 ,  A61L2300/64 ,  A61L2430/20 ,  C12N5/0691 ,  C12N5/0697 ,  C12N2502/1323 ,  C12N2502/1329 ,  C12N2502/28

摘要： Provided is a method for producing a cardiac tissue spheroid or vascular tissue spheroid formed from a mixture of a myocardial cell or smooth muscle cell and at least one type of cell selected from a vascular endothelial cell and fibroblast, and a three dimensional cardiac tissue structure or three dimensional vascular tissue structure which are characterized by combining or laminating said spheroid.

摘要翻译： 本发明提供一种由心肌细胞或平滑肌细胞和至少一种选自血管内皮细胞和成纤维细胞的细胞的混合物形成的心脏组织球状体或血管组织球形体的制造方法，以及三维心脏组织结构或 三维血管组织结构，其特征在于组合或层压所述球体。

公开(公告)号：US20220213424A1

公开(公告)日：2022-07-07

申请号：US17438614

申请日：2020-03-13

申请人： SAGA UNIVERSITY

发明人： Koichi NAKAYAMA

IPC分类号： C12M1/12 ,  B33Y80/00 ,  C12N5/071 ,  G01N33/50

摘要： A cell culture apparatus having a cell-holding container and a pinholder-shaped member comprising needle-shaped bodies arranged on a substrate, wherein a protruding part is formed in the center of the bottom surface of the cell-holding container, a recessed part is formed between the center and a side wall, and through-holes through which the needle-shaped bodies penetrate are established on the bottom surface of the recessed part; the needle-shaped bodies are arranged in correspondence with the positions of the through-holes; and the pinholder-shaped member is arranged, such that a tip-side portion of each of the needle-shaped bodies penetrates through the corresponding through-hole from the bottom surface side or upper surface side of the cell-holding container.

公开(公告)号：US20240318113A1

公开(公告)日：2024-09-26

申请号：US18578809

申请日：2022-07-19

申请人： SAGA UNIVERSITY

发明人： Koichi NAKAYAMA ,  Anna Maria NAKAMURA

IPC分类号： C12M1/12 ,  C12N5/00

CPC分类号： C12M25/14 ,  C12N5/0062

摘要： A culture system that comprises: a support for stacking cell aggregates, said support being provided with needle-shaped parts that are arranged on a substrate like a kenzan (flower frog needles), in which the outermost needle-shaped parts on the substrate are arranged so that the distance from the needle-shaped parts to the outer edge of the substrate is shorter than the distance between adjacent needle-shaped parts; a culture tank for housing a culture unit in which cell aggregates are stacked on the support; a liquid culture medium container; and a plurality of liquid culture medium supply pipes that supply a liquid culture medium from the liquid culture medium container to the culture unit, wherein each of the plurality of liquid culture medium supply pipes is positioned between the needle-shaped part.