公开(公告)号：US20240352426A1

公开(公告)日：2024-10-24

申请号：US18686708

申请日：2022-08-25

Applicant: Vanderbilt University ,  The United States as Represented by The Department of Veterans Affairs

Inventor： Lauren E. WOODARD ,  Julie BEJOY

IPC: C12N5/071 ,  A61K35/545

CPC classification number: C12N5/0686 ,  A61K35/545 ,  C12N2500/38 ,  C12N2501/119 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/165 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2501/91 ,  C12N2506/45 ,  C12N2533/52

Abstract: Disclosed herein is a method for producing intermediate mesodermal cells from human pluripotent stem cells. Also disclosed is a method for producing nephron progenitor cells from human pluripotent stem cells. Also disclosed is a method for producing podocytes. Also disclosed is a method for treating a kidney disease in a subject, that involves administering to the subject a therapeutically effective amount of the produced intermediate mesodermal cells, nephron progenitor cells, and/or podocytes.

公开(公告)号：US20240352418A1

公开(公告)日：2024-10-24

申请号：US18571568

申请日：2022-06-17

Applicant: Megakaryon Corporation

Inventor： Yukitaka ITO ,  Sachiyo MISUMI

IPC: C12N5/078

CPC classification number: C12N5/0644 ,  C12N2501/727 ,  C12N2501/998 ,  C12N2510/04

Abstract: A method for improving the amount of platelets produced. The production method is a method for producing multinucleated megakaryocyte cells with enhanced platelet production capability, the method including a multinucleation step of inducing multinucleated megakaryocyte cells through multinucleation of pre-multinucleated megakaryocyte cells in the presence of a growth factor of the pre-multinucleated megakaryocyte cells. In the multinucleation step, the multinucleated megakaryocyte cells are induced by suppressing the activity of at least one protein selected from the group consisting of an aryl hydrocarbon receptor (AhR), a Rho-associated kinase (ROCK), dual-specificity tyrosine phosphorylation-regulated kinases (DYRK), and myosin 2, and multinucleating the pre-multinucleated megakaryocyte cells in the presence of harmine.

公开(公告)号：US20240352413A1

公开(公告)日：2024-10-24

申请号：US18639238

申请日：2024-04-18

Applicant: THE GENERAL HOSPITAL CORPORATION

Inventor： Jayaraj RAJAGOPAL ,  Hongmei Mou

IPC: C12N5/074 ,  A61K9/00 ,  A61K35/42 ,  A61L27/38 ,  A61L27/56 ,  A61L27/58 ,  C12N5/071 ,  G01N33/68 ,  G03B21/28

CPC classification number: C12N5/0607 ,  A61K35/42 ,  A61L27/3834 ,  A61L27/3882 ,  A61L27/56 ,  A61L27/58 ,  C12N5/0678 ,  C12N5/068 ,  C12N5/0688 ,  C12N5/0689 ,  G01N33/6884 ,  G03B21/28 ,  A61K9/0073 ,  A61L2430/22 ,  C12N2501/115 ,  C12N2501/117 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/385 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2506/02 ,  C12N2506/45 ,  C12N2533/90

Abstract: Provided herein are methods and compositions relating, in part, to the generation of human progenitor cells committed to the lung lineage and uses of such cells for treatment of lung diseases/disorders or injury to the lung. Whether an adult stem cell can be isolated from human adult lung remains controversial in the art and at present, methods for isolating and using adult lung stem cells from humans lack reproducibility. Thus, the methods and compositions described herein are advantageous over the present state of knowledge in the art and permit the generation of human lung progenitor cells for treatment, tissue engineering, and screening assays.

公开(公告)号：US12123023B2

公开(公告)日：2024-10-22

申请号：US17503143

申请日：2021-10-15

Applicant: President and Fellows of Harvard College

Inventor： Douglas A. Melton ,  Mads Gurtler

IPC: C12N5/071 ,  A61K35/39 ,  G01N33/50

CPC classification number: C12N5/0678 ,  A61K35/39 ,  C12N5/0676 ,  G01N33/507 ,  C12N2501/16 ,  C12N2501/727

Abstract: Disclosed herein are methods for generating SC-β cells, and isolated populations of SC-β cells for use in various applications, such as cell therapy.

公开(公告)号：US12123019B2

公开(公告)日：2024-10-22

申请号：US17071627

申请日：2020-10-15

Applicant: Whitehead Institute for Biomedical Research ,  Dana-Farber Cancer Institute, Inc.

Inventor： Thorold W Theunissen ,  Nathanael S. Gray ,  Rudolf Jaenisch

IPC: C12N5/0735 ,  C12N5/074

CPC classification number: C12N5/0606 ,  C12N5/0696 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/165 ,  C12N2501/727 ,  C12N2501/999

Abstract: The present disclosure provides compounds of any one of Formulae (A) to (L). The present disclosure also provides compositions, uses, and methods that include or involve a compound described herein, a serine/threonine-protein kinase B-Raf (BRAF) inhibitor, an epidermal growth factor receptor (EGFR) inhibitor, a vascular endothelial growth factor 1 (VEGFR1) inhibitor, a fibroblast growth factor receptor 1 (FGFR1) inhibitor, or a combination thereof. The compounds, compositions, uses, and methods are useful in changing the pluripotency state of a vertebrate cell to a more naïve state.

公开(公告)号：US20240336897A1

公开(公告)日：2024-10-10

申请号：US18687608

申请日：2022-08-26

Applicant: CORNELL UNIVERSITY

Inventor： Vimal Selvaraj ,  Viju Pillai

IPC: C12N5/074

CPC classification number: C12N5/0696 ,  C12N2501/15 ,  C12N2501/16 ,  C12N2501/2306 ,  C12N2501/235 ,  C12N2501/727 ,  C12N2510/00 ,  C12N2533/90

Abstract: Described in several exemplary embodiments herein are compositions and methods for reprogramming cells, such as somatic cells. Also described in several exemplary embodiments herein are induced pluripotent stem cells (iPSCs), particularly ruminant iPSCs.

公开(公告)号：US20240318144A1

公开(公告)日：2024-09-26

申请号：US18433553

申请日：2024-02-06

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Yaqub HANNA ,  Noa NOVERSHTERN ,  Yoach RAIS

IPC: C12N5/074 ,  C12N5/00 ,  C12N5/0735

CPC classification number: C12N5/0696 ,  C12N5/0037 ,  C12N5/0606 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2501/01 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/235 ,  C12N2501/415 ,  C12N2501/50 ,  C12N2501/60 ,  C12N2501/71 ,  C12N2501/72 ,  C12N2501/727 ,  C12N2501/73 ,  C12N2501/999 ,  C12N2506/00

Abstract: A culture medium is disclosed which comprises STAT3 activator, an ERK1/2 inhibitor and an Axin stabilizer, and optionally also a PKC inhibitor. Cell cultures comprising same and uses thereof are also disclosed.

公开(公告)号：US12098386B2

公开(公告)日：2024-09-24

申请号：US16462346

申请日：2017-12-13

Applicant: L'OREAL

Inventor： Theebah Sellathurai ,  Khalid Bakkar

IPC: C12N5/071 ,  C12N5/0775

CPC classification number: C12N5/0628 ,  C12N5/0666 ,  C12N5/0697 ,  C12N2501/40 ,  C12N2501/727 ,  C12N2501/999 ,  C12N2502/092 ,  C12N2502/094 ,  C12N2502/13 ,  C12N2502/1376 ,  C12N2513/00

Abstract: The invention relates to the use of germ cells for obtaining a micro hair follicle and to the use thereof for evaluating the effect of cosmetic, pharmaceutical or dermatological products and also for the prophylactic or therapeutic treatment of a state of reduced pilosity.

公开(公告)号：US20240301346A1

公开(公告)日：2024-09-12

申请号：US18270924

申请日：2022-05-11

Applicant: TREEFROG THERAPEUTICS

Inventor： Maxime FEYEUX ,  Philippe Joseph COHEN

IPC: C12N5/00 ,  C12N5/074

CPC classification number: C12N5/0012 ,  C12N5/0696 ,  C12N2501/727 ,  C12N2510/00 ,  C12N2513/00 ,  C12N2533/74

Abstract: The invention relates to a three-dimensional cellular microcompartment or a three-dimensional cellular microcompartment assembly comprising at least one external hydrogel layer and inside said external layer at least one layer of cells and/or at least one cellular base layer, of which less than 20% of the total population of cells present in the microcompartment or in the microcompartment assembly are cells having at least one mutation.
The invention also relates to a method for producing such a microcompartment or microcompartment assembly.

公开(公告)号：US12084680B2

公开(公告)日：2024-09-10

申请号：US17149713

申请日：2021-01-14

Applicant: Wisconsin Alumni Research Foundation

Inventor： Ethan Scott Lippmann ,  Neha Sehgal ,  Randolph Scott Ashton

IPC: C12N5/0797

CPC classification number: C12N5/0623 ,  C12N2500/38 ,  C12N2501/119 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2501/999

Abstract: Described herein are chemically defined, adherent culture protocols for generating functional motor neurons characteristic of diverse hindbrain and spinal cord regions, with high efficiency.