公开(公告)号：US20230167413A1

公开(公告)日：2023-06-01

申请号：US17921174

申请日：2021-04-30

申请人： CEDARS-SINAI MEDICAL CENTER

发明人： Barry R. Stripp ,  Bindu Konda ,  Apoorva Mulay

IPC分类号： C12N5/071 ,  G01N33/50 ,  G01N1/30 ,  G01N33/569

CPC分类号： C12N5/0689 ,  G01N33/5044 ,  G01N1/30 ,  G01N33/56966 ,  C12N2513/00 ,  C12N2509/10 ,  C12N2501/727 ,  C12N2501/15

摘要： The in vitro organoid model is a major technological breakthrough and an essential tool to study the basic biology of an organ system and for the development of various clinical applications for disease intervention. Organoids can self-renew and exhibit similarities in function as of their tissue of origin. Here, a step-by-step protocol is described to isolate region-specific progenitors from the human lung and generate 3D organoid cultures as an experimental and validation tool.

公开(公告)号：US20180208903A1

公开(公告)日：2018-07-26

申请号：US15865574

申请日：2018-01-09

申请人： TRUSTEES OF BOSTON UNIVERSITY

发明人： Darrell N. KOTTON ,  Katherine B. McCauley ,  Finn HAWKINS

IPC分类号： C12N5/074 ,  C12N5/0735 ,  C07K14/47 ,  C12N15/86 ,  A61P11/00

CPC分类号： C12N5/0696 ,  A61P11/00 ,  C07K14/4702 ,  C12N5/0606 ,  C12N5/0689 ,  C12N15/1138 ,  C12N15/86 ,  C12N2310/14 ,  C12N2501/415 ,  C12N2506/45

摘要： The technology described herein relates to methods and kits for directed differentation of primordial NKX2-1+ lung progenitors along proximal differentiation pathways into functional airway epithelial cells and airway organoids (“bronchospheres”) or along distal lineage pathways using modulation of Wnt signaling. Other aspects relate cell lines, methods, assays and kits comprising airway epithelial cells, and assays for diagnosing a disease that affects swelling of the bronchospheres, and/or for assessing genetic lesions and/or drugs for treating the the disease, where the disease is cystic fibrosis. Other aspects relate to personalized medicine and methods of treatment of cystic fibrosis using the airway epithelial cells.

公开(公告)号：US20170165301A1

公开(公告)日：2017-06-15

申请号：US15360561

申请日：2016-11-23

申请人： AAL Scientifics, Inc.

发明人： Piero ANVERSA ,  Annarosa LERI

IPC分类号： A61K35/42 ,  C12N5/071

CPC分类号： A61K35/42 ,  A61K45/06 ,  C12N5/0689

摘要： Embodiments of the invention relate to human stem cells and their therapeutic use in the treatment and/or prevention of lung diseases. Provided herein are compositions comprising c-kit positive human lung stem cells and methods of preparing and using c-kit positive human lung stem cells for the treatment and/or prevention of lung diseases.

公开(公告)号：US09528091B2

公开(公告)日：2016-12-27

申请号：US12308351

申请日：2007-06-18

申请人： Joseph H. Lin ,  Judith A. Shizuru

发明人： Joseph H. Lin ,  Judith A. Shizuru

IPC分类号： C12N5/00 ,  C12N15/85 ,  C12N5/071

CPC分类号： C12N5/0689

摘要： Substantially enriched mammalian lung endothelial and epithelial progenitor cell populations are provided. Methods are provided for the isolation and in vivo differentiation of such lung progenitor cells. The progenitor cells are obtained from lung tissue, including fetal and adult tissues. The cells are useful in transplantation, for experimental evaluation, and as a source of lineage and cell specific products, including mRNA species useful in identifying genes specifically expressed in these cells, and as targets for the discovery of factors or molecules that can affect them.

摘要翻译： 提供了大量富集的哺乳动物肺内皮细胞和上皮祖细胞群体。 提供了用于分离和体内分化这些肺祖细胞的方法。 祖细胞从肺组织获得，包括胎儿和成人组织。 这些细胞可用于移植，用于实验评估，以及作为谱系和细胞特异性产物的来源，包括用于鉴定在这些细胞中特异性表达的基因的mRNA，以及作为发现可影响它们的因子或分子的靶标。

公开(公告)号：US20140329318A1

公开(公告)日：2014-11-06

申请号：US14371627

申请日：2013-01-11

申请人： THE GENERAL HOSPITAL CORPORATION

发明人： Jayaraj Rajagopal ,  Hongmei Mou

IPC分类号： C12N5/074

CPC分类号： C12N5/0607 ,  A61K9/0073 ,  A61K35/42 ,  A61L27/3834 ,  A61L27/3882 ,  A61L27/56 ,  A61L27/58 ,  A61L2430/22 ,  C12N5/0678 ,  C12N5/068 ,  C12N5/0688 ,  C12N5/0689 ,  C12N2501/115 ,  C12N2501/117 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/385 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2506/02 ,  C12N2506/45 ,  C12N2533/90 ,  G01N33/6884

摘要： Provided herein are methods and compositions relating, in part, to the generation of human progenitor cells committed to the lung lineage and uses of such cells for treatment of lung diseases/disorders or injury to the lung. Whether an adult stem cell can be isolated from human adult lung remains controversial in the art and at present, methods for isolating and using adult lung stem cells from humans lack reproducibility. Thus, the methods and compositions described herein are advantageous over the present state of knowledge in the art and permit the generation of human lung progenitor cells for treatment, tissue engineering, and screening assays.

摘要翻译： 本文提供的方法和组合物部分涉及致力于肺部谱系的人祖细胞的产生以及这种细胞用于治疗肺部疾病/病症或肺损伤的用途。 成人干细胞是否可以从人类成年肺分离，在本领域仍然是有争议的，目前用于分离和使用人类成年肺干细胞的方法缺乏再现性。 因此，本文所述的方法和组合物优于本领域现有知识状态并且允许产生用于治疗，组织工程和筛选测定的人肺祖细胞。

公开(公告)号：US20080292677A1

公开(公告)日：2008-11-27

申请号：US11298543

申请日：2005-12-09

申请人： Joaquin Cortiella ,  Larry Bonassar ,  Koji Kojima ,  Joan E. Nichols ,  Ronald P. Mlcak ,  Jean A. Niles

发明人： Joaquin Cortiella ,  Larry Bonassar ,  Koji Kojima ,  Joan E. Nichols ,  Ronald P. Mlcak ,  Jean A. Niles

IPC分类号： A61K35/42 ,  A61K9/10 ,  C12N5/06 ,  A61F2/00 ,  A61F2/02

CPC分类号： A61K9/0073 ,  A61K35/42 ,  A61L27/3804 ,  A61L27/3882 ,  C12N5/0689 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2533/40

摘要： Somatic lung progenitor cell/polymer constructs are disclosed along with methods for isolating somatic lung progenitor cells from adult mammals, seeding the cells onto or into polymeric scaffolds and allowing the cells to differentiate and proliferate into functional lung tissue/polymer implants. A method for treating lung disease, disorders or injuries is also disclosed.

摘要翻译： 公开了体细胞肺祖细胞/聚合物构建体以及从成年哺乳动物分离体细胞肺祖细胞的方法，将细胞接种到聚合支架上或聚合支架中，并允许细胞分化并增殖成功能性肺组织/聚合物植入物。 还公开了一种治疗肺部疾病，病症或损伤的方法。

公开(公告)号：US20060270035A1

公开(公告)日：2006-11-30

申请号：US11432775

申请日：2006-05-11

申请人： Menno Kielman

发明人： Menno Kielman

IPC分类号： C12N5/02

CPC分类号： C12N5/0606 ,  A61K35/12 ,  C07K14/475 ,  C12N5/0689 ,  C12N2501/415

摘要： The invention relates to a method for at least in part inhibiting differentiation of stem cells in a population of mammalian cells comprising up-regulating a Wnt-signaling pathway to a differentiation-inhibiting level in the population of cells. The invention increases at the same time the number of stem cells in a population of mammalian cells compared to a reference population, and induces, at least in part, apoptosis in mesenchymal cells in a population of mammalian cells. The invention also discloses a method for selective differentiation of a stem cell, comprising controlling the level of Wnt pathway activation. The invention is used for the proliferation and subsequent differentiation of embryonic stem cells and lung stem cells, and for ex vivo lung explant cultivation.

摘要翻译： 本发明涉及至少部分抑制哺乳动物细胞群体中干细胞分化的方法，包括将Wnt信号通路上调至细胞群体中的分化抑制水平。 与参考群体相比，本发明同时增加哺乳动物细胞群体中的干细胞的数量，并且至少部分地诱导哺乳动物细胞群体中的间充质细胞的凋亡。 本发明还公开了一种用于选择性分化干细胞的方法，其包括控制Wnt途径活化的水平。 本发明用于胚胎干细胞和肺干细胞的增殖和随后的分化，并用于离体肺外植体培养。

公开(公告)号：US20240352413A1

公开(公告)日：2024-10-24

申请号：US18639238

申请日：2024-04-18

申请人： THE GENERAL HOSPITAL CORPORATION

发明人： Jayaraj RAJAGOPAL ,  Hongmei Mou

IPC分类号： C12N5/074 ,  A61K9/00 ,  A61K35/42 ,  A61L27/38 ,  A61L27/56 ,  A61L27/58 ,  C12N5/071 ,  G01N33/68 ,  G03B21/28

CPC分类号： C12N5/0607 ,  A61K35/42 ,  A61L27/3834 ,  A61L27/3882 ,  A61L27/56 ,  A61L27/58 ,  C12N5/0678 ,  C12N5/068 ,  C12N5/0688 ,  C12N5/0689 ,  G01N33/6884 ,  G03B21/28 ,  A61K9/0073 ,  A61L2430/22 ,  C12N2501/115 ,  C12N2501/117 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/385 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2506/02 ,  C12N2506/45 ,  C12N2533/90

摘要： Provided herein are methods and compositions relating, in part, to the generation of human progenitor cells committed to the lung lineage and uses of such cells for treatment of lung diseases/disorders or injury to the lung. Whether an adult stem cell can be isolated from human adult lung remains controversial in the art and at present, methods for isolating and using adult lung stem cells from humans lack reproducibility. Thus, the methods and compositions described herein are advantageous over the present state of knowledge in the art and permit the generation of human lung progenitor cells for treatment, tissue engineering, and screening assays.

公开(公告)号：US11814651B2

公开(公告)日：2023-11-14

申请号：US16490786

申请日：2018-03-07

申请人： FUJITA ACADEMY

发明人： Hideyuki Saya ,  Yoshimi Arima ,  Takashi Semba ,  Akiyoshi Kasuga

IPC分类号： C12N5/071 ,  A01K67/027 ,  A61K49/00 ,  C12N5/095 ,  C12Q1/02

CPC分类号： C12N5/0689 ,  A01K67/027 ,  A61K49/0008 ,  C12N5/0695 ,  C12Q1/025 ,  A01K2207/12 ,  A01K2227/105 ,  A01K2267/0331 ,  C12N2500/30 ,  C12N2500/90 ,  C12N2501/11 ,  C12N2501/117 ,  C12N2506/27 ,  C12N2513/00

摘要： Provided is an epithelial tissue stem cell derived from an adult, which lacks at least one tumor suppressor gene.

公开(公告)号：US11739299B2

公开(公告)日：2023-08-29

申请号：US17088090

申请日：2020-11-03

申请人： The Trustees of Columbia University in the City of New York

发明人： Hans-Willem Snoeck ,  Ya-Wen Chen

IPC分类号： C12N5/071

CPC分类号： C12N5/0689 ,  C12N2501/11 ,  C12N2501/117 ,  C12N2501/119 ,  C12N2501/155 ,  C12N2501/33 ,  C12N2501/727 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2506/45

摘要： The present disclosure provides methods for generating lung progenitor cells, and populations of cells made using the methods. The lung progenitors and related compositions can be used as therapeutic treatments for various pulmonary disorders or related injuries.