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    CELL CULTIVATION METHOD, CELL SUPPORT COMPOSITE PRODUCTION METHOD, CULTIVATED CELLS, AND CELL SUPPORT COMPOSITE
    2.
    发明公开
    CELL CULTIVATION METHOD, CELL SUPPORT COMPOSITE PRODUCTION METHOD, CULTIVATED CELLS, AND CELL SUPPORT COMPOSITE 审中-公开

    公开(公告)号：US20240263144A1

    公开(公告)日：2024-08-08

    申请号：US18636926

    申请日：2024-04-16

    申请人： NIKKISO CO., LTD.

    发明人： Fumihiko KITAGAWA

    IPC分类号： C12N5/071

    CPC分类号： C12N5/0686 ,  C12N2500/30 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/90

    摘要： A cell support composite includes a substrate, a coating agent layer and cultivated cells. The coating agent layer covers at least a portion of the substrate, and contains one or more laminin molecules, a basement membrane matrix mixture, collagen molecules, and fragments of any of these. The cultivated cells adhere to the substrate with the coating agent layer being interposed therebetween. The cultivated cells are produced by cultivating primary cultured kidney cells dedifferentiated in a state of being non-adherent to a culture vessel, forming aggregates of the kidney cells, then cultivating the kidney cells in a state of having formed aggregates, and restoring physiological functions of the kidney cells. The primary cultured kidney cells include primary cultured renal proximal tubular epithelial cells, and a percentage of the primary cultured renal proximal tubular epithelial cells in the primary cultured kidney cells is 83% or more.

    METHOD FOR PRODUCING ANTIBODY POPULATION
    4.
    发明公开
    METHOD FOR PRODUCING ANTIBODY POPULATION 审中-公开

    公开(公告)号：US20240254534A1

    公开(公告)日：2024-08-01

    申请号：US18632968

    申请日：2024-04-11

    申请人： PRESTIGE BIOLOGICS CO., LTD.

    发明人： Joo Yang PARK ,  Kyung Hee JUNG ,  Ji Sung PARK ,  Jin Young KU

    IPC分类号： C12P21/00 ,  C07K16/24 ,  C12N5/00

    CPC分类号： C12P21/005 ,  C07K16/241 ,  C12N5/00 ,  C07K2317/21 ,  C12N2500/30 ,  C12N2500/60 ,  C12N2523/00

    摘要： Provided is a method for producing an antibody population and, specifically, to an antibody population of desired quality that is produced by culturing recombinant cells expressing antibodies under elaborately controlled culturing conditions such as pH and culture temperature, and a method for effectively producing an antibody population of high quality with excellent biological activity. The present method can effectively prepare an antibody population having desired proportions of main active antibodies and isomeric antibodies and an antibody population having a desired glycan structure by adjusting pH, culture temperature, and/or lactic acid supply. In addition, the present method can produce high-quality antibodies having excellent biological activity capable of reaching desired therapeutic efficacy when therapeutic monoclonal antibodies are generated. In particular, with respect to the manufacture of biosimilar drugs, it is possible to effectively manufacture antibodies of the same or very similar quality to the original drug by elaborately adjusting the culture conditions.

    Serum-free complete medium for inducing differentiation of a mesenchymal stem cell to a corneal epithelial cell
    5.
    发明授权
    Serum-free complete medium for inducing differentiation of a mesenchymal stem cell to a corneal epithelial cell 有权

    公开(公告)号：US12012611B2

    公开(公告)日：2024-06-18

    申请号：US17283491

    申请日：2020-05-25

    申请人： QINGDAO RESTORE BIOTECHNOLOGY CO., LTD.

    发明人： Bingqiang Zhang ,  Mengmeng Chen ,  Wei Zou ,  Xueqi Fu

    IPC分类号： C12N5/079

    CPC分类号： C12N5/0621 ,  C12N2500/30 ,  C12N2501/14 ,  C12N2501/235 ,  C12N2501/37 ,  C12N2506/1346

    摘要： A serum-free complete medium for inducing differentiation of a mesenchymal stem cell to a corneal epithelial cell in the field of differentiation induction of stem cells, prepared by the following method: uniformly mixing the serum-free complete medium, containing 5-10 μmol of resveratrol, 2-4 μmol of icariin, 1-3 nmol of aspirin, 1-3 nmol of parathyroid hormone, 5-10 nmol of hydrocortisone, 1-3 mg of rapamycin, 2-10 μg of testosterone, 2-10 μg of EPO, 2-10 μg of LIF and the balance of a corneal epithelial cell serum-free medium in per 1 L; and then performing sterilization by filtration. The disclosure uses resveratrol and icariin in combination with aspirin, parathyroid hormone, hydrocortisone, rapamycin, testosterone and growth factors to cooperatively induce directional differentiation, uses nontoxic induction components, is high in induction efficiency and short in induction time, and achieves high induced corneal epithelial cell activity, no cell transplantation rejection, no ethical problem and high safety.

    POLY-3-HYDROXYALKANOATES HAVING VINYL MOIETIES AND METHOD OF PRODUCING SUCH
    10.
    发明公开
    POLY-3-HYDROXYALKANOATES HAVING VINYL MOIETIES AND METHOD OF PRODUCING SUCH 审中-公开

    公开(公告)号：US20240060099A1

    公开(公告)日：2024-02-22

    申请号：US18030970

    申请日：2021-10-11

    申请人： POLYFERM CANADA INC.

    发明人： Bruce RAMSAY ,  Juliana RAMSAY ,  Araceli FLORES-SANCHEZ

    IPC分类号： C12P7/625 ,  C12N9/02 ,  C12N9/10 ,  C12N9/88 ,  C12N15/74

    CPC分类号： C12P7/625 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/88 ,  C12N15/74 ,  C12N2500/30 ,  C12Y103/08001 ,  C12Y402/01017

    摘要： A method of producing polyhydroxyalkanoate (PHA) copolymers comprising both short-chain-length (scl) and medium-chain-length (mcl) subunits, wherein some of the mcl subunits bear reactive vinyl groups, is provided. The method comprises providing cells comprising (i) a PHA synthase (phaC) gene encoding a particular class I poly(3-hydroxyalkanoate) polymerase, and (ii) a phaJ gene encoding a particular (R)-specific enoyl-CoA hydratase, and cultivating said cells in a growth medium comprising an alkenoic acid. In one example, cells grown in the presence of a mixture of decanoic acid and undecen-10-enoic acid yielded a copolymer comprising units of 3-hydroxybutyrate (94.73 mol %), 3-hydroxyhexanoate (1.51 mol %), 3-hydroxyoctanoate (2.33 mol %), 3-hydroxydecanoate (0.69 mol %) and 3-hydroxyhept-6-enoate (0.73 mol %). The presence of acrylic acid as a 13-oxidation inhibitor in the growth medium led to an increase in both the mcl-PHA subunit content and vinyl subunit content. For example, a copolymer containing about 1.2 mol % 3-hydroxyhept-6-enoate and about 0.5 mol % 3-hydroxynon-8-enoate was obtained at a concentration of 40 mM acrylic acid. PHA accumulation of up to 57.2% (w/w of cell dry weight) are reported. The scl-mcl PHAs with subunits bearing reactive vinyl groups are expected to be useful in a variety of applications, for instance for covalently linking bioactive molecules.