公开(公告)号：US20240084248A1

公开(公告)日：2024-03-14

申请号：US18514880

申请日：2023-11-20

申请人： Regeneron Pharmaceuticals, Inc.

发明人： Wei Xue ,  John Chen ,  Carolyn Scott ,  Theodore Loney ,  Nathaniel Golden

IPC分类号： C12N5/00

CPC分类号： C12N5/0037 ,  C12N5/0043 ,  C07K2319/30 ,  C12N2500/30 ,  C12N2500/40 ,  C12N2500/46

摘要： Cell culture media are provided herein as are methods of using the media for cell culture and protein production from cells.

公开(公告)号：US11891510B2

公开(公告)日：2024-02-06

申请号：US16645915

申请日：2018-12-06

申请人： Sigma-Aldrich Co. LLC

发明人： Scott Wilson ,  Kevin Kent ,  Chandana Sharma

IPC分类号： C08L71/02 ,  C08L71/00 ,  C12N5/00

CPC分类号： C08L71/02 ,  C08L71/00 ,  C12N5/0043

摘要： Described herein are poloxamer compositions for use as a shear protectant in cell culture and methods for preparing and using such compositions.

公开(公告)号：US20190136202A1

公开(公告)日：2019-05-09

申请号：US16242463

申请日：2019-01-08

申请人： AJINOMOTO CO., INC.,

发明人： Sho SENDA ,  Tomomi YOSHIDA ,  Satoru OKAMOTO ,  Yoko KURIYAMA ,  Manabu KITAZAWA ,  Ikue HARATA ,  Nao SUGIMOTO

IPC分类号： C12N5/074 ,  C12N5/0735 ,  C12N5/00

CPC分类号： C12N5/0696 ,  C12N5/0043 ,  C12N5/0606 ,  C12N2500/36 ,  C12N2500/46 ,  C12N2500/84 ,  C12N2533/90

摘要： Cultivating a pluripotent stem cell in a medium comprising at least one member selected from the group consisting of ethanolamine, an ethanolamine analog, and a s pharmaceutically acceptable salt thereof, and which is substantially free of β-mercaptoethanol or contains β-mercaptoethanol at a concentration of not more than 9 μM, and the like, is effective for the proliferation of a pluripotent stem cell while maintaining an undifferentiated state.

公开(公告)号：US20170283505A1

公开(公告)日：2017-10-05

申请号：US15623226

申请日：2017-06-14

申请人： Genentech, Inc.

发明人： Martin GAWLITZEK ,  Shun LUO ,  Christina Teresa BEVILACQUA

IPC分类号： C07K16/28 ,  C07K16/18 ,  C07K14/705 ,  C12N5/00 ,  C07K16/22

CPC分类号： C07K16/2878 ,  C07K14/70575 ,  C07K16/18 ,  C07K16/22 ,  C07K2317/14 ,  C07K2319/30 ,  C12N5/0018 ,  C12N5/0043 ,  C12N2500/32 ,  C12N2500/33 ,  C12N2500/90

摘要： The present invention relates generally to glutamine-free cell culture media supplemented with asparagine. The invention further concerns the production of recombinant proteins, such as antibodies, in asparagine-supplemented glutamine-free mammalian cell culture.

公开(公告)号：US09714293B2

公开(公告)日：2017-07-25

申请号：US14670079

申请日：2015-03-26

申请人： GENENTECH, INC.

发明人： Martin Gawlitzek ,  Shun Luo ,  Christina Teresa Bevilacqua

IPC分类号： C12N5/18 ,  C07K16/28 ,  C12N5/00 ,  C07K16/18 ,  C07K16/22 ,  C07K14/705

CPC分类号： C07K16/2878 ,  C07K14/70575 ,  C07K16/18 ,  C07K16/22 ,  C07K2317/14 ,  C07K2319/30 ,  C12N5/0018 ,  C12N5/0043 ,  C12N2500/32 ,  C12N2500/33 ,  C12N2500/90

摘要： The present invention relates generally to glutamine-free cell culture media supplemented with asparagine. The invention further concerns the production of recombinant proteins, such as antibodies, in asparagine-supplemented glutamine-free mammalian cell culture.

公开(公告)号：US09664671B2

公开(公告)日：2017-05-30

申请号：US13949310

申请日：2013-07-24

申请人： Nissan Chemical Industries, Ltd. ,  Kyoto University

发明人： Taito Nishino ,  Tatsuro Kanaki ,  Ayako Otani ,  Koichiro Saruhashi ,  Misayo Tomura ,  Takehisa Iwama ,  Masato Horikawa ,  Norio Nakatsuji ,  Tomomi Otsuji

IPC分类号： C12N5/071 ,  C12N5/09 ,  G01N33/50

CPC分类号： C12N5/0043 ,  C12N5/005 ,  C12N5/067 ,  C12N5/0693 ,  C12N2500/14 ,  C12N2500/34 ,  C12N2531/00 ,  C12N2533/80 ,  G01N33/5011 ,  G01N33/5014 ,  G01N33/5044 ,  G01N33/5067

摘要： The present invention provides a culture method of cells and/or tissues including culturing cells and/or tissues in a suspended state by using a medium composition wherein indeterminate structures are formed in a liquid medium, the structures are uniformly dispersed in the solution and substantially retain the cells and/or tissues without substantially increasing the viscosity of the solution, thus affording an effect of preventing sedimentation thereof, and the like.

公开(公告)号：US20160369316A1

公开(公告)日：2016-12-22

申请号：US15235453

申请日：2016-08-12

申请人： Baxalta Innovations GmbH

发明人： Manfred Reiter ,  Wolfgang Mundt ,  Friedrich Dorner ,  Leopold Grillberger ,  Artur Mitterer

IPC分类号： C12P21/00 ,  C12N5/00 ,  C07K14/755

CPC分类号： C12P21/00 ,  C07K14/755 ,  C12N5/0037 ,  C12N5/0043 ,  C12N5/005 ,  C12N5/0682 ,  C12N2500/05 ,  C12N2500/32 ,  C12N2500/38 ,  C12N2500/46 ,  C12N2500/60 ,  C12N2500/76 ,  C12N2500/92

摘要： A medium is described for the protein-free and serum-free cultivation of cells, especially mammalian cells, whereby the medium contains a proportion of soy hydrolysate.

公开(公告)号：US09404085B2

公开(公告)日：2016-08-02

申请号：US13651151

申请日：2012-10-12

申请人： ES Cell International Pte Ltd

发明人： Bruce Paul Davidson ,  Ralph Eberhard Graichen ,  Robert Zweigerdt ,  Xiuqin Xu ,  Christine Lindsay Mummery ,  William Sun

IPC分类号： C12N5/02 ,  C12N5/077 ,  G01N33/50 ,  C12N5/00 ,  A61K35/12

CPC分类号： C12N5/0657 ,  A61K35/12 ,  C12N5/0043 ,  C12N2500/25 ,  C12N2500/99 ,  C12N2501/02 ,  C12N2501/105 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/155 ,  C12N2501/33 ,  C12N2501/999 ,  C12N2502/13 ,  C12N2502/30 ,  C12N2506/02 ,  C12N2510/00 ,  G01N33/502 ,  G01N33/5061

摘要： The present invention relates to the induction of differentiation in human embryonic stem (hES) cells to cardiomyocytes and factors such as prostaglandin I2 (PGI2), Fgf 9, Bmp6, Bmp4, Scf, Igf2, and insulin that influence the process of differentiation to cardiomyocytes. Media that is appropriate for the induction of differentiation of cardiomyocytes from hES cells is also provided wherein the media contains these factors. Genes that are upregulated in the process of cardiomyocyte differentiation are also provided.

摘要翻译： 本发明涉及诱导人胚胎干细胞分化为心肌细胞和诸如影响心肌细胞分化过程的前列腺素I2（PGI2），Fgf9，Bmp6，Bmp4，Scf，Igf2和胰岛素的因子 。 还提供了适合诱导心肌细胞从hES细胞分化的培养基，其中培养基含有这些因子。 还提供了在心肌细胞分化过程中上调的基因。

公开(公告)号：US09222075B2

公开(公告)日：2015-12-29

申请号：US14268859

申请日：2014-05-02

申请人： Baxter International Inc. ,  Baxter Healthcare SA

发明人： Leopold Grillberger ,  Manfred Reiter ,  Wolfgang Mundt ,  Friedrich Dorner

IPC分类号： C12N1/20 ,  C12N5/071 ,  C12N5/00

CPC分类号： C12N5/0043 ,  C12N5/0682 ,  C12N2500/46 ,  C12N2500/74 ,  C12N2500/76 ,  C12N2500/92

摘要： The present invention relates to animal protein-free cell culture media comprising polyamines and a plant- and/or yeast-derived hydrolysate. The invention also relates to animal protein-free culturing processes, wherein cells can be cultivated, propagated and passaged without adding supplementary animal proteins in the culture medium. These processes are useful in cultivating cells, such as recombinant cells or cells infected with a virus, and for producing biological products by cell culture processes.

摘要翻译： 本发明涉及含有多胺和植物和/或酵母衍生的水解产物的无动物蛋白的细胞培养基。 本发明还涉及无动物蛋白质的培养方法，其中细胞可培养，繁殖和传代，而不在培养基中添加补充动物蛋白。 这些方法可用于培养细胞，例如重组细胞或感染病毒的细胞，以及通过细胞培养方法生产生物产物。

公开(公告)号：US09040297B2

公开(公告)日：2015-05-26

申请号：US12309817

申请日：2007-08-02

申请人： Michal Amit ,  Joseph Itskovitz-Eldor

发明人： Michal Amit ,  Joseph Itskovitz-Eldor

IPC分类号： C12N5/08 ,  C12N5/0735 ,  C12N5/00

CPC分类号： C12N5/0606 ,  C12N5/0031 ,  C12N5/0037 ,  C12N5/0043 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/2306 ,  C12N2501/235 ,  C12N2533/12 ,  C12N2533/90

摘要： A method of expanding and maintaining human embryonic stem cells (ESCs) in an undifferentiated state by culturing the ESCs in a suspension culture under culturing conditions devoid of substrate adherence is provided. Also provided are a method of deriving ESC lines in the suspension culture and methods of generating lineage-specific cells from ESCs which were expanded in the suspension culture of the present invention.

摘要翻译： 提供了通过在没有底物粘附的培养条件下在悬浮培养物中培养ESCs来扩增和维持处于未分化状态的人胚胎干细胞（ESCs）的方法。 还提供了在悬浮培养物中得到ESC系的方法以及在本发明的悬浮培养物中扩增的ESC产生谱系特异性细胞的方法。