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1.发明公开公开(公告)号：US20240352426A1
公开(公告)日：2024-10-24
申请号：US18686708
申请日：2022-08-25
发明人： Lauren E. WOODARD , Julie BEJOY
IPC分类号： C12N5/071 , A61K35/545
CPC分类号： C12N5/0686 , A61K35/545 , C12N2500/38 , C12N2501/119 , C12N2501/155 , C12N2501/16 , C12N2501/165 , C12N2501/415 , C12N2501/727 , C12N2501/91 , C12N2506/45 , C12N2533/52
摘要： Disclosed herein is a method for producing intermediate mesodermal cells from human pluripotent stem cells. Also disclosed is a method for producing nephron progenitor cells from human pluripotent stem cells. Also disclosed is a method for producing podocytes. Also disclosed is a method for treating a kidney disease in a subject, that involves administering to the subject a therapeutically effective amount of the produced intermediate mesodermal cells, nephron progenitor cells, and/or podocytes.
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公开(公告)号：US20240344029A1
公开(公告)日：2024-10-17
申请号：US18632921
申请日：2024-04-11
发明人： Allen EHRLICHER , Ajinkya GHAGRE
IPC分类号： C12N5/077 , C12N5/0793
CPC分类号： C12N5/0654 , C12N5/0619 , C12N5/0653 , C12N2500/30 , C12N2500/38 , C12N2500/42 , C12N2506/1346 , C12N2527/00 , C12N2531/00
摘要： There is provided a method of selectively differentiating mesenchymal stem cells into a first predetermined cell lineage associated with the nuclear localization of Yes-associated protein (YAP) or into a second predetermined cell lineage associated with the cytoplasmic localization of YAP. The curvature of the nucleus is controlled to have a maximum nuclear curvature (Kmax) of at least 0.5 μm−1 to select for the first predetermined cell lineage, or a Kmax that does not exceed 0.5 μm−1 to select for the second predetermined cell lineage. The mesenchymal stem cells having a controlled nuclear curvature are incubated in a media with or without differentiation additives to obtain the first predetermined cell lineage or the second predetermined cell lineage.
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公开(公告)号：US20240327783A1
公开(公告)日：2024-10-03
申请号：US18435956
申请日：2024-02-07
发明人： Joseph White , Matthew Smonskey , Thomas Mellody
CPC分类号： C12N5/0018 , C12N5/0603 , C12N5/0604 , C12N2500/32 , C12N2500/34 , C12N2500/36 , C12N2500/38
摘要： Described herein are formulations of cell culture media and supplements that are stable when stored at room temperature. Embodiments described herein encompass dry granulated or agglomerated cell culture media with carbohydrate sources and vitamins that are physically separated. Further embodiments described herein are dry granulated or agglomerated cell culture media with a carbohydrate source that is separately agglomerated or powdered. Additional embodiments are dry granulated or agglomerated cell culture media with a carbohydrate source that is separately agglomerated or powdered and optionally contains vitamins. In another embodiment, the culture media or supplement is a dry granulated or agglomerated cell culture media containing granulated or agglomerated or powdered carbohydrates and vitamins in one or more of a liquid-, powdered-, granulated-, agglomerated-, coated-, or encapsulated-form.
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公开(公告)号：US12104170B2
公开(公告)日：2024-10-01
申请号：US18002828
申请日：2021-06-28
发明人： Cynthia Bamdad , Kevin R. Yi , Scott T. Moe , Thomas Jeon
IPC分类号： C12N5/0793 , A61K35/30
CPC分类号： C12N5/0619 , A61K35/30 , C12N2500/38 , C12N2501/10 , C12N2501/415 , C12N2501/999 , C12N2506/02 , C12N2506/45
摘要： The present application discloses a method of producing dopaminergic neurons from human stem cells by adding or increasing concentration of vitamin into neuro basal media at approximately Day 20+/−3 of a protocol for differentiating pluripotent stem cells into dopaminergic neurons.
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公开(公告)号：US20240318144A1
公开(公告)日：2024-09-26
申请号：US18433553
申请日：2024-02-06
发明人： Yaqub HANNA , Noa NOVERSHTERN , Yoach RAIS
IPC分类号： C12N5/074 , C12N5/00 , C12N5/0735
CPC分类号： C12N5/0696 , C12N5/0037 , C12N5/0606 , C12N2500/38 , C12N2500/90 , C12N2501/01 , C12N2501/115 , C12N2501/125 , C12N2501/15 , C12N2501/155 , C12N2501/16 , C12N2501/235 , C12N2501/415 , C12N2501/50 , C12N2501/60 , C12N2501/71 , C12N2501/72 , C12N2501/727 , C12N2501/73 , C12N2501/999 , C12N2506/00
摘要： A culture medium is disclosed which comprises STAT3 activator, an ERK1/2 inhibitor and an Axin stabilizer, and optionally also a PKC inhibitor. Cell cultures comprising same and uses thereof are also disclosed.
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公开(公告)号：US20240301351A1
公开(公告)日：2024-09-12
申请号：US18662758
申请日：2024-05-13
CPC分类号： C12N5/0621 , A61K35/30 , C12N2500/02 , C12N2500/38 , C12N2501/115 , C12N2501/15 , C12N2501/16 , C12N2506/02 , C12N2533/52
摘要： A population of human polygonal RPE cells is disclosed. At least 95% of the cells thereof co-express premelanosome protein (PMEL17) and cellular retinaldehyde binding protein (CRALBP), wherein the trans-epithelial electrical resistance of the cells is greater than 100 ohms. Methods of generating same are also disclosed.
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公开(公告)号：US12087399B2
公开(公告)日：2024-09-10
申请号：US16482946
申请日：2018-02-01
申请人： Sapphiros AI Bio LLC
IPC分类号： G16B20/20 , C12N5/00 , C12Q1/6881
CPC分类号： G16B20/20 , C12N5/0018 , C12Q1/6881 , C12N2500/30 , C12N2500/32 , C12N2500/36 , C12N2500/38 , C12Q2600/156 , Y10S423/05
摘要： Described herein are various systems and methods for customizing cell culture media for optimized cell proliferation based on genetic traits of the cells. Factors of the cells, such as genetics, can determine the optimum cell culture conditions for growth and use of individual cells in vitro. In certain embodiments, genetics can determine sensitivity from a toxic perspective. In certain embodiments, genetics can determine what concentration(s) of growth factor(s) and nutrient(s) are required for optimum growth.
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公开(公告)号：US12084680B2
公开(公告)日：2024-09-10
申请号：US17149713
申请日：2021-01-14
IPC分类号： C12N5/0797
CPC分类号： C12N5/0623 , C12N2500/38 , C12N2501/119 , C12N2501/415 , C12N2501/727 , C12N2501/999
摘要： Described herein are chemically defined, adherent culture protocols for generating functional motor neurons characteristic of diverse hindbrain and spinal cord regions, with high efficiency.
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公开(公告)号：US20240293479A1
公开(公告)日：2024-09-05
申请号：US18593178
申请日：2024-03-01
IPC分类号： A61K35/741 , A61K9/19 , C12N1/20 , C12R1/46
CPC分类号： A61K35/741 , A61K9/19 , C12N1/205 , C12N2500/16 , C12N2500/34 , C12N2500/38 , C12N2500/50 , C12N2500/74 , C12N2500/84 , C12N2501/998 , C12R2001/46
摘要： Provided is a method of producing a freeze-dried probiotic composition as well as a method of producing a probiotic raw material which involves culturing of a Streptococcus salivarius strain. Furthermore, a corresponding probiotic composition and a corresponding probiotic raw material is provided.
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公开(公告)号：US20240287460A1
公开(公告)日：2024-08-29
申请号：US18573148
申请日：2022-06-23
CPC分类号： C12N5/0658 , C12N1/12 , C12N5/0025 , C12N2500/05 , C12N2500/32 , C12N2500/34 , C12N2500/38 , C12N2500/76 , C12N2500/90 , C12N2502/70
摘要： The present invention provides a method for producing a composition for culturing animal cells, wherein the method includes: (1) a step in which an algae is mixed with a solid acid catalyst and an algae extract is obtained by heating; and (2) a step in which the algae extract is added to a medium for culturing animal cells and the concentration of the algae extract is adjusted. The present invention also provides a recycling/culturing method for algae and animal cells including: (i) a step in which a waste liquid (a first waste liquid) previously used to culture animal cells is used to culture algae; (ii) a step in which the algae is collected, mixed with a solid catalyst, and heated, and an algae extract is thereby obtained; (iii) a step in which the algae extract is added to the waste liquid (a second waste liquid) previously used to culture algae in (i), the concentration of the algae extract is adjusted, and a composition for culturing animal cells is produced; and (iv) a step in which animal cells are cultured using the composition for culturing animal cells.
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