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公开(公告)号：US12227762B2
公开(公告)日：2025-02-18
申请号：US17437411
申请日：2020-03-11
Inventor： Cheil Moon , Won Bae Jeon , Sam Hwan Kim , Seong Kyoon Choi
IPC: C12N5/0793
Abstract: The present invention relates to a method for culturing neural stem cells into spheroids, the method including: culturing neural stem cells in a culture vessel coated with a protein containing a VGVPG pentapeptide and an RGD integrin receptor ligand; and isolating the neural stem cells that are aggregated and formed into spheroids during the culturing.
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公开(公告)号：US12227761B2
公开(公告)日：2025-02-18
申请号：US16624756
申请日：2018-06-19
Applicant: UPM-KYMMENE CORPORATION
Inventor： Markus Nuopponen , Lauri Paasonen , Susanna Narkilahti , Tiina Joki , Laura Ylä-Outinen
IPC: C12N5/0793 , C08B15/02
Abstract: The present invention is related to methods and materials for culturing expanding cells in a three-dimensional culture. The material comprises plant-derived anionic nanofibrillar cellulose, wherein the anionic nanofibrillar cellulose is in a form of hydrogel. The invention also provides methods for producing materials and compositions comprising plant-derived anionic nanofibrillar cellulose.
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公开(公告)号：US12221720B2
公开(公告)日：2025-02-11
申请号：US16763681
申请日：2018-11-13
Applicant: The Broad Institute, Inc. , Massachusetts Institute of Technology , President and Fellows of Harvard College
Inventor： Naomi Habib , Aviv Regev , Eugene Drokhlyansky , Anindita Basu , Inbal Avraham-Davidi , Orit Rozenblatt-Rosen , David A. Weitz
IPC: C40B50/06 , A61K35/30 , C12N5/0793 , C12N5/0797 , C12Q1/6806 , C12Q1/6881 , C40B30/06 , C12N5/00
Abstract: Techniques Nuc-seq, Div-Seq, and Dronc-Seq are allow for unbiased analysis of any complex tissue. Nuc-Seq, a scalable single nucleus RNA-Seq method, can sensitively identify closely related cell types, including within the adult hippocampus. Div-seq combines Nuc-Seq with EdU-mediated labeling of proliferating cells, allowing tracking of transcriptional dynamics of newborn neurons in an adult neurogenic region in the hippocampus. Dronc-Seq uses a microfluidic device to co-encapsulate individual nuclei in reverse emulsion aqueous droplets in an oil medium together with one uniquely barcoded mRNA-capture bead.
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公开(公告)号：US12221624B2
公开(公告)日：2025-02-11
申请号：US16755312
申请日：2018-10-17
Applicant: National University of Singapore
Inventor： Karl Tryggvason , Hwee Goon Tay , Aida Moreno Moral
IPC: C12N5/0793 , C12P21/02
Abstract: Retinal progenitors and mature photoreceptor cells can be produced by differentiating human embryonic stem cells on a surface having a laminin matrix thereon made of two laminins One laminin is laminin-521, and the other laminin is either laminin-323 or laminin-523. Stem cells plated on this substrate can be differentiated using various cell culture mediums to obtain the retinal progenitors and mature photoreceptor cells.
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公开(公告)号：US20250009805A1
公开(公告)日：2025-01-09
申请号：US18708322
申请日：2022-11-08
Applicant: CORNELL UNIVERSITY
Inventor： David Lin
IPC: A61K35/30 , A61P25/00 , C12N5/0793
Abstract: Described in several embodiments herein are olfactory sensory neuron (OSN) derived extracellular vehicles (EVs), formulations thereof, and uses thereof. In some embodiments, the OSN derived extracellular vesicles are effective to increase the growth of neurons during regeneration. In some embodiments, the OSN derived EVs and/or formulations thereof are used to promote nerve regeneration, particularly peripheral nerve regeneration, and to treat nerve injury.
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公开(公告)号：US12188020B2
公开(公告)日：2025-01-07
申请号：US17323826
申请日：2021-05-18
Applicant: Ionis Pharmaceuticals, Inc.
Inventor： Frank Rigo
IPC: C07H21/04 , A61K31/7088 , A61P25/28 , C12N5/0793 , C12N15/113
Abstract: Provided herein are methods for decreasing Ataxin-2 mRNA expression. Such methods are useful to ameliorate symptoms of Ataxin-2 associated diseases. Such Ataxin-2 associated diseases include amyotrophic lateral sclerosis (ALS). Such symptoms include loss of motor function, reduced CMAP amplitude, denervation, and loss of motor neurons.
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公开(公告)号：US20250002846A1
公开(公告)日：2025-01-02
申请号：US18711270
申请日：2022-12-23
Applicant: INDUSTRY FOUNDATION OF CHONNAM NATIONAL UNIVERSITY , AJOU UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION
Inventor： JANG HO KIM , YUN HOON CHOUNG , SUN HO PARK , YEON JU KIM
IPC: C12N5/0793 , C12M1/32
Abstract: In a method for producing an inner ear organoid, stem cells and a culture medium are treated on a microwell plate whose an inner circumferential surface has a curved surface inclined downward, the stem cells are seeded in each microwell, and the stem cells seeded in each microwell are cultured. Using the method, cell aggregates for production of inner ear organoids may be easily formed, and uniform and mature inner ear organoids may be produced using the specific initial cell number control method and growth factor treatment method of the present invention, and may be used for inner ear disease modeling and drug screening.
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公开(公告)号：US20240409891A1
公开(公告)日：2024-12-12
申请号：US18819435
申请日：2024-08-29
Applicant: Regeneron Pharmaceuticals, Inc.
Inventor： Marine Prissette , Matthew Koss , Mathieu Desclaux , John McWhirter , Arijit Bhowmick , David Frendewey , Brian Zambrowicz , Claudia Racioppi
IPC: C12N5/0793 , A61K9/00 , A61K48/00 , C12N15/113
Abstract: BANF1, PPP2CA, and ANKLE2 were identified as genes that promote tau aggregation when disrupted. Improved tauopathy models such as cells, tissues, or animals having mutations in or inhibition of expression of BANF1 and/or PPP2CA and/or ANKLE2 are provided. Methods of using such improved tauopathy models for assessing therapeutic candidates for the treatment of a tauopathy, methods of making the improved tauopathy models, and methods of accelerating or exacerbating tau aggregation in a tauopathy model are also provided.
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公开(公告)号：US20240409890A1
公开(公告)日：2024-12-12
申请号：US18739113
申请日：2024-06-10
Applicant: Li Gan et al. , Shiaoching Gong , Celeste Parra Bravo , Zeping Zhao
Inventor： Li Gan et al. , Shiaoching Gong , Celeste Parra Bravo , Zeping Zhao
IPC: C12N5/0793 , A61K31/343 , A61P25/28 , C07K14/47 , C12N5/00 , C12N9/22 , C12N15/113 , G01N33/50
Abstract: Provided herein are iPSC lines engineered to express 4R-tau and 4R-tau carrying the P301S MAPT mutation when differentiated into neurons. 4R-P301S neurons display progressive Tau inclusions upon seeding with Tau fibrils and recapitulate features of tauopathy phenotypes, including shared transcriptomic signatures, autophagic body accumulation, and impaired neuronal activity. A CRISPRi screening of genes associated with Tau pathobiology identified over 500 genetic modifiers of Tau-seeding-induced Tau propagation, including retromer VPS29 and the UFMylation cascade as top modifiers. In AD brains, the UFMylation cascade is altered in neurofibrillary-tangle-bearing neurons. Inhibiting the UFMylation cascade suppressed seeding-induced Tau propagation. Also provided herein is a platform to identify novel therapeutic strategies for 4R tauopathy.
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公开(公告)号：US20240400984A1
公开(公告)日：2024-12-05
申请号：US18182664
申请日：2023-03-13
Applicant: Shanghai GeneMagic BioSciences Co., Ltd.
Inventor： Haibo Zhou , Canbin Feng , Zhengzheng Xu , Ni Gao , Xinde Hu
IPC: C12N5/0793 , C12N9/22
Abstract: This invention provides the use of one or more inhibitors reducing the expression or activity of the genes, the genes's RNA, or the genes's encoding proteins to treat the diseases associated with neuronal functional loss or the neuronal death. The genes are selected from: Plpp7, Fam126a, Gprc5c, Tmed4, Tle6, Psmd5, Mastl, Ssr3, Rhoa, Rfx8, Rbm10, Hnrupa3, Prpf6, Pou3f3, Ncoal, Ccdc8, Adck1, Gjb2, Smad9, Nr2e1, Atp10b, Nid1, Tmcc3, Rad21, Amigo1, Cep192, Sepp1, Klf12, Nxf1, Trp53inp2, Phlpp1, Ptpdc1, Pebp1, Gm22174, Gm26117, Mir873a, Mir1900, Gm22414, Khdc4, Hnrnpa0, Hnrnph2, Srrm1, Hnrnpf, Srsf4, Mbnl1, Zbtb42, Kcmf1, Gtf2i, Chgb, Fos, Kat2a, Tsg101, Hmgb4, Junb, Cdx2, Cers2, Rhox6, Thap3, Zscan25.
