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公开(公告)号:US12087399B2
公开(公告)日:2024-09-10
申请号:US16482946
申请日:2018-02-01
申请人: Sapphiros AI Bio LLC
IPC分类号: G16B20/20 , C12N5/00 , C12Q1/6881
CPC分类号: G16B20/20 , C12N5/0018 , C12Q1/6881 , C12N2500/30 , C12N2500/32 , C12N2500/36 , C12N2500/38 , C12Q2600/156 , Y10S423/05
摘要: Described herein are various systems and methods for customizing cell culture media for optimized cell proliferation based on genetic traits of the cells. Factors of the cells, such as genetics, can determine the optimum cell culture conditions for growth and use of individual cells in vitro. In certain embodiments, genetics can determine sensitivity from a toxic perspective. In certain embodiments, genetics can determine what concentration(s) of growth factor(s) and nutrient(s) are required for optimum growth.
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公开(公告)号:US12115037B2
公开(公告)日:2024-10-15
申请号:US16490147
申请日:2018-03-02
申请人: Sapphiros AI Bio LLC
IPC分类号: A61D19/04 , A61B17/435 , C12N5/0735 , C12N5/076 , C12N9/22 , C12N15/11 , C12N15/90
CPC分类号: A61D19/04 , A61B17/435 , C12N5/061 , C12N5/0611 , C12N9/22 , C12N15/11 , C12N15/907 , C12N2310/20 , C12N2506/45 , C12N2800/80
摘要: Reserves of immortalized genetic material are stored in a bank for providing a resource (e.g., artificial gametes) for couples (e.g., same sex couples) to produce biologically-related children. The reserves provide the ability to derive sperm from induced pluripotent stem cells (iPSCs) of one partner and/or eggs from iPSCs of the other partner. For example, a biological sample is stored as iPSCs that can be used to generate an unlimited supply of genetic material (e.g., artificial gametes for conception) when needed by a user (e.g., a year or more after the sample is provided, e.g., 5 years or more after the sample is provided). Such a bank is helpful for an individual in a same sex (or infertile) couple who desires to have biological children at some point during his/her lifetime, but does not plan to have children in the immediate timeframe, for example.
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3.
公开(公告)号:US20240026303A1
公开(公告)日:2024-01-25
申请号:US18202050
申请日:2023-05-25
申请人: Sapphiros AI Bio LLC
IPC分类号: C12N5/074 , G16B45/00 , G16B35/10 , G01N33/569
CPC分类号: C12N5/0696 , G16B45/00 , G16B35/10 , G01N33/56966 , C12N2501/999 , C12N2510/00 , C12N2510/02
摘要: Described herein are various systems, methods, and apparatus for systematic creation of isolated homogeneous colonies of cells from vector-based lineages. The vector-based lineages may originate from multiple types of viral vector families (e.g., Paramyx-oviridae, Retroviridae, Parvoviridae) or non-natural engineered vectors or a plurality of vector combinations, for example. In certain embodiments, the isolated homogeneous colonies of cells are vector-free sub-colonies; in other embodiments, the isolated homogeneous colonies of cells are homogeneous vector sub-colonies. In other embodiments, vector mixed sub-colonies are created. The disclosed systems, methods, and apparatus are useful for inducible pluripotent stem cell (iPSC) production and work by selectively binding to one or more corresponding protein markers expressed on the surface of a cell that indicate that cellular reprogramming has occurred. Software is used to automate the purification and isolation of the iPSCs produced.
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公开(公告)号:US11692174B2
公开(公告)日:2023-07-04
申请号:US16484167
申请日:2018-02-08
申请人: Sapphiros AI Bio LLC
IPC分类号: G01N33/48 , C12N5/074 , G16B45/00 , G16B35/10 , G01N33/569
CPC分类号: C12N5/0696 , G01N33/56966 , G16B35/10 , G16B45/00 , C12N2501/999 , C12N2510/00 , C12N2510/02
摘要: Described herein are various systems, methods, and apparatus for systematic creation of isolated homogeneous colonies of cells from vector-based lineages. The vector-based lineages may originate from multiple types of viral vector families (e.g., Paramyx-oviridae, Retroviridae, Parvoviridae) or non-natural engineered vectors or a plurality of vector combinations, for example. In certain embodiments, the isolated homogeneous colonies of cells are vector-free sub-colonies; in other embodiments, the isolated homogeneous colonies of cells are homogeneous vector sub-colonies. In other embodiments, vector mixed sub-colonies are created. The disclosed systems, methods, and apparatus are useful for inducible pluripotent stem cell (iPSC) production and work by selectively binding to one or more corresponding protein markers expressed on the surface of a cell that indicate that cellular reprogramming has occurred. Software is used to automate the purification and isolation of the iPSCs produced.
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