公开(公告)号：US12054742B2

公开(公告)日：2024-08-06

申请号：US16251740

申请日：2019-01-18

Applicant: THE REGENTS OF THE UNIVERSITY OF MICHIGAN

Inventor： Rajesh Rao ,  Qiang Li

IPC: C12N5/0735 ,  C12N5/00 ,  C12N5/077 ,  C12N5/079 ,  C12N5/0793 ,  C12N15/85 ,  C12Q1/02

CPC classification number: C12N5/0606 ,  C12N5/0037 ,  C12N5/062 ,  C12N5/0621 ,  C12N5/0657 ,  C12N15/85 ,  C12Q1/025 ,  C12N2500/99 ,  C12N2501/22 ,  C12N2501/2303 ,  C12N2501/385 ,  C12N2501/998 ,  C12N2506/02 ,  C12N2510/00 ,  C12N2513/00 ,  C12N2533/90

Abstract: The disclosure relates to methods for producing mesodermal lineage cells, cardiac lineage cells, hematopoietic lineage cells, retinal lineage cells, and combinations thereof. In some aspects, the disclosure relates to methods of producing mixed tissue organoids comprising retinal lineage cells and cardiac lineage cells. When interaction of WDR5 protein at the binding pocket/interaction surface with RBBP5/MYC/KANSL2 is disrupted in the embryonic stem cell for a set period of time after differentiation of the embryonic stem cell has begun, and the disruption of that interaction is subsequently removed, differentiation of the embryonic stem cell to a mesodermal lineage cell is obtained. Such mesodermal lineage cells may, in some methods of the disclosure, be subsequently differentiated into cardiac lineage cells and hematopoietic lineage cells. The disclosure also relates to method for producing mixed lineage organoids, comprising retinal and mesodermal, including cardiac, lineage cells in a single organoid. The disclosure also relates to cells and organoids obtained by the methods, uses of the cells and organoids, and kits comprising the cells and/or reagents for producing them.

公开(公告)号：US12049646B2

公开(公告)日：2024-07-30

申请号：US18395362

申请日：2023-12-22

Applicant: President and Fellows of Harvard College

Inventor： Quinn P. Peterson ,  Felicia J. Pagliuca ,  Douglas A. Melton ,  Jeffrey Robert Millman ,  Michael Saris Segel ,  Mads Gurtler

IPC: C12N5/071 ,  A61K35/39 ,  A61P3/10 ,  A61P5/48 ,  A61P5/50 ,  C12N5/0735 ,  C12N5/074

CPC classification number: C12N5/0678 ,  A61K35/39 ,  A61P3/10 ,  A61P5/48 ,  A61P5/50 ,  C12N5/0606 ,  C12N5/0676 ,  C12N5/0696 ,  C12N2501/11 ,  C12N2501/117 ,  C12N2501/15 ,  C12N2501/16 ,  C12N2501/375 ,  C12N2501/385 ,  C12N2501/395 ,  C12N2501/40 ,  C12N2501/41 ,  C12N2501/727 ,  C12N2501/998 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2506/03 ,  C12N2506/22 ,  C12N2506/45

Abstract: Disclosed herein are methods, compositions, kits, and agents useful for inducing β cell maturation, and isolated populations of SC-β cells for use in various applications, such as cell therapy.

公开(公告)号：US12018278B2

公开(公告)日：2024-06-25

申请号：US16618531

申请日：2017-05-31

Applicant: BeiHao Stem Cell and Regenerative Medicine Research Institute Co., Ltd. ,  HONG GUAN LTD.

Inventor： Hongkui Deng ,  Xiang Li ,  Defang Liu ,  Yantao Ma ,  Xiaomin Du

IPC: C12N5/073

CPC classification number: C12N5/0603 ,  C12N2501/01 ,  C12N2501/065 ,  C12N2501/15 ,  C12N2501/385 ,  C12N2501/999

Abstract: Provided are chemical inducers of lineage reprogramming (CiLR) which include glycogen synthase kinase inhibitors, TGFβ receptor inhibitors, cyclic AMP agonists or histone acetylators. Also provided is a method of inducing lineage reprograming in a partially or completely differentiated cell of a first type into a cell with characteristics of a second and different lineage. The method includes: contacting a cell with the CiLR for a sufficient period of time to result in reprograming the cell into a modified XEN-like cell which is subsequently programmed into a cell with characteristics of a second and different lineage.

公开(公告)号：US20240158743A1

公开(公告)日：2024-05-16

申请号：US18422961

申请日：2024-01-25

Applicant: Trailhead Biosystems Inc.

Inventor： Nooshin Amini

IPC: C12N5/079

CPC classification number: C12N5/0622 ,  C12N2501/155 ,  C12N2501/385 ,  C12N2501/41 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2506/02 ,  C12N2506/45 ,  C12N2533/50

Abstract: Methods for generating pre-oligodendrocyte progenitor cells (pre-OPCs) and oligodendrocyte progenitor cells (OPCs) from human pluripotent stem cells are provided using chemically-defined culture media that allow for generation of pre-OPCs and OPCs in as little as three days. Culture media, isolated cell populations and kits are also provided.

公开(公告)号：US20240117303A1

公开(公告)日：2024-04-11

申请号：US18199415

申请日：2023-05-19

Applicant: Trailhead Biosystems Inc.

Inventor： Nooshin AMINI

IPC: C12N5/0793

CPC classification number: C12N5/0619 ,  C12N2501/13 ,  C12N2501/155 ,  C12N2501/385 ,  C12N2501/415 ,  C12N2501/42 ,  C12N2501/727 ,  C12N2501/815 ,  C12N2501/91 ,  C12N2506/02 ,  C12N2506/45

Abstract: Methods for generating human midbrain immature neurons and mature dopaminergic neurons from neural progenitor cells are provided. The immature and mature midbrain neurons are obtained from neural progenitors such as committed midbrain neural stem cells (NSCs) and midbrain neural progenitor cells (midbrain NPCs), which themselves are generated from human pluripotent stem cells. The methods for generating midbrain immature and mature neurons use chemically-defined culture media that allow for generation of mature dopaminergic neurons in as little as 23 days. Culture media, isolated cell populations and kits are also provided.

公开(公告)号：US11905530B2

公开(公告)日：2024-02-20

申请号：US17127221

申请日：2020-12-18

Applicant: ViaCyte, Inc.

Inventor： Alan D. Agulnick ,  Olivia Kelly ,  Yuki Ohi ,  Allan Robins ,  Thomas Schulz

IPC: C12N5/071 ,  A61K35/39

CPC classification number: C12N5/0676 ,  A61K35/39 ,  C12N2500/25 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/117 ,  C12N2501/16 ,  C12N2501/195 ,  C12N2501/385 ,  C12N2501/41 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2506/02 ,  C12N2506/45

Abstract: Disclosed herein are cell culture compositions, for example, pancreatic cell culture compositions, derived from dedifferentiated human reprogrammed pluripotent stem cells, such as induced pluripotent stem (iPS) cells, and methods for producing and using such cell culture compositions.

公开(公告)号：US20230313143A1

公开(公告)日：2023-10-05

申请号：US17935574

申请日：2022-09-26

Applicant: AVITA INTERNATIONAL LTD. ,  FUNDAÇÃO BUTANTAN

Inventor： Irina Kerkis

IPC: C12N5/0775 ,  C12N5/0793 ,  C12N5/071 ,  A61K35/30 ,  A61K35/32 ,  C12N5/0797 ,  A61K35/28 ,  A61K45/06 ,  C12N5/079

CPC classification number: C12N5/0664 ,  A61K35/28 ,  A61K35/30 ,  A61K35/32 ,  A61K45/06 ,  C12N5/0619 ,  C12N5/0621 ,  C12N5/0623 ,  C12N5/0678 ,  C12N2500/25 ,  C12N2500/38 ,  C12N2500/84 ,  C12N2500/90 ,  C12N2501/385 ,  C12N2501/39 ,  C12N2501/392 ,  C12N2501/395 ,  C12N2506/1361 ,  C12N2533/76

Abstract: The present invention is directed to therapeutic multifunctional immature dental pulp stem cells (IDPSCs), and IDPSCs multi-lineage compositions. The invention is further directed to the use of IDPSCs and compositions to reduce the risk of and/or treat degenerative diseases or for other medicinal and aesthetic purposes.

公开(公告)号：US11760977B2

公开(公告)日：2023-09-19

申请号：US16301671

申请日：2017-05-24

Applicant: SALK INSTITUTE FOR BIOLOGICAL STUDIES

Inventor： Ronald Evans ,  Michael Downes ,  Annette Atkins ,  Eiji Yoshihara ,  Ruth Yu

IPC: C12N5/071 ,  G01N33/50 ,  A61K35/39

CPC classification number: C12N5/0677 ,  A61K35/39 ,  G01N33/5088 ,  C12N2501/117 ,  C12N2501/16 ,  C12N2501/385 ,  C12N2501/395 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2501/999 ,  C12N2502/1382 ,  C12N2502/28 ,  C12N2503/02 ,  C12N2506/02 ,  C12N2506/45 ,  C12N2513/00 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/90 ,  G01N2800/04 ,  G01N2800/7028

Abstract: The invention features pancreatic islet and pancreatic organoids, and cell cultures and methods that are useful for the rapid and reliable generation of pancreatic islet and pancreatic islet organoids. The invention also features methods of treating pancreatic diseases and methods of identifying agents that are useful for treatment of pancreatic diseases, such as type 2 diabetes and pancreatic cancer, using the pancreatic islet and pancreatic organoids of the invention.

公开(公告)号：US11708562B2

公开(公告)日：2023-07-25

申请号：US16598920

申请日：2019-10-10

Applicant: Janssen Biotech, Inc.

Inventor： Alireza Rezania ,  Jean Xu

IPC: C12N5/071 ,  A61K35/39

CPC classification number: C12N5/0676 ,  A61K35/39 ,  C12N5/067 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/16 ,  C12N2501/385 ,  C12N2501/415 ,  C12N2501/42 ,  C12N2501/70 ,  C12N2501/727 ,  C12N2506/02 ,  C12N2533/90

Abstract: The present invention provides methods to promote the differentiation of pluripotent stem cells. In particular, the present invention provides an improved method for the formation of pancreatic endoderm, pancreatic hormone expressing cells and pancreatic hormone secreting cells. The present invention also provides methods to promote the differentiation of pluripotent stem cells without the use of a feeder cell layer.

公开(公告)号：US20230210906A1

公开(公告)日：2023-07-06

申请号：US17570266

申请日：2022-01-06

Applicant: Jay GOPALAKRISHNAN ,  Elke GABRIEL ,  Aruljothi MARIAPPAN ,  Anand RAMANI

Inventor： Jay GOPALAKRISHNAN ,  Elke GABRIEL ,  Aruljothi MARIAPPAN ,  Anand RAMANI

IPC: A61K35/30 ,  C12N5/0793 ,  C12N5/079 ,  C12Q1/02

CPC classification number: A61K35/30 ,  C12N5/0619 ,  C12N5/0621 ,  C12N5/062 ,  C12Q1/025 ,  C12N2506/45 ,  C12N2513/00 ,  C12N2501/385 ,  C12N2501/155 ,  C12N2501/15 ,  C12N2501/16

Abstract: The present invention relates to 3D brain organoids, uses thereof, methods and culture medium for generating such organoids. An aspect of the invention provides brain organoids and methods of generating such organoids with bilaterally symmetric optic vesicles, containing both neuronal and non-neuronal cell types, and exhibiting functional circuitry. These organoids can be generated within short time intervals (e.g., 50 days) and therefore are useful for medical modelling and applications.