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公开(公告)号：US20240141445A1
公开(公告)日：2024-05-02
申请号：US17778927
申请日：2020-11-16
Inventor： Stefan KUBICEK , Andreas REICHER
IPC: C12Q1/6897 , C12N15/10 , G01N33/50
CPC classification number: C12Q1/6897 , C12N15/1086 , G01N33/5038 , G01N2500/10
Abstract: The present invention relates to a method for monitoring the effect of an environmental factor on the proteome of a cell. Furthermore, cell populations are provided that comprise multiple cells each comprising an inserted tag sequence in an intron of said cell, wherein the tag is inserted in-frame with the preceding exonic sequence and wherein the intron into which the tag is inserted is different between cells.
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公开(公告)号：US11906510B2
公开(公告)日：2024-02-20
申请号：US17207295
申请日：2021-03-19
Inventor： Anthony T. Le
CPC classification number: G01N33/5038 , G01N30/06 , G01N30/72 , G01N2030/027
Abstract: Disclosed herein are methods of analyzing a biological sample comprising: separating components of the biological sample via reversed-phase (RP) chromatography to obtain an elute; subjecting the elute to separation via ion-exchange (IEX) chromatography or mixed-mode IEX chromatography; and detecting the separated compounds to determine the components of the biological sample. Also disclosed are devices comprising a reversed-phase (RP) chromatography column in communication with an ion-exchange (IEX) chromatography column or mixed-mode IEX chromatography column, wherein there is no switching valve between the columns.
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公开(公告)号：US11899014B2
公开(公告)日：2024-02-13
申请号：US16906669
申请日：2020-06-19
Inventor： Virginia Wood Cornish , Nili Ostrov , Miguel Jimenez , Sonja Billerbeck
IPC: G01N33/566 , C12Q1/02 , G01N33/74 , G01N33/50 , C12Q1/00 , C12Q1/06 , G01N33/569
CPC classification number: G01N33/566 , C12Q1/005 , C12Q1/02 , C12Q1/025 , C12Q1/06 , G01N33/5023 , G01N33/5038 , G01N33/569 , G01N33/5695 , G01N33/56911 , G01N33/56916 , G01N33/56961 , G01N33/74 , G01N2333/726
Abstract: The present invention provides sensor cells comprising a receptor that binds to an analyte indicative of the presence of an agent, where binding of the analyte to the receptor triggers a detection event that is indicative of the presence of the agent. In certain embodiments, the detection event is appearance of a reporter detectable by the naked eye. The present invention also provides uses of such sensor cells for detecting the presence of an agent in a sample.
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公开(公告)号：US11819276B2
公开(公告)日：2023-11-21
申请号：US17939566
申请日：2022-09-07
Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA
Inventor： Robert Vince , Swati Sudhakar More
IPC: G01J3/28 , A61B3/12 , G01N21/31 , G01J3/00 , G01J3/40 , G01N21/25 , A61B3/14 , A61K38/06 , G01N33/50 , A61B5/00
CPC classification number: A61B3/12 , A61B3/14 , A61B5/4848 , A61K38/063 , G01J3/00 , G01J3/28 , G01J3/40 , G01N21/25 , G01N21/31 , G01N33/5038
Abstract: Described herein is the use of a visible near infrared (VNIR) hyperspectral imaging system as a non-invasive diagnostic tool for early detection of Alzheimer's disease (AD). Also described herein is the use of a VNIR hyperspectral imaging system in high throughput screening of potential therapeutics against AD.
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公开(公告)号：US20230341379A1
公开(公告)日：2023-10-26
申请号：US18188665
申请日：2023-03-23
Applicant: Genentech Inc.
Inventor： Katherine Kozak , Josefa dela Cruz Chuh
CPC classification number: G01N33/505 , G01N33/5011 , G01N33/5038 , G01N33/582
Abstract: The present disclosure provides methods and systems for high throughput assays for testing immune cell engager molecules and potential immune cell engager molecules. In some embodiments, multiple parameters, for example, in connection with engagement of tumor cells by immune cells such as T cells and in connection with tumor cell death, may be analyzed from the same samples in the assays, and, in some cases, may be analyzed simultaneously. In some embodiments, the methods and systems allow for determining the kinetics of various parameters.
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公开(公告)号：US20180237741A1
公开(公告)日：2018-08-23
申请号：US15958976
申请日：2018-04-20
Applicant: Cedars-Sinai Medical Center
Inventor： Dan Gazit , Gadi Pelled , Zulma Gazit , Dmitriy Sheyn
CPC classification number: B01L3/5027 , A61K49/0019 , A61K49/005 , A61K51/02 , B01L3/5085 , C07K14/78 , C12N5/0062 , C12N5/0075 , C12N5/0654 , C12N5/0662 , C12N5/0663 , C12N5/0696 , C12N5/0697 , G01N33/5038 , G01T1/1644 , G01T1/2985
Abstract: Microfluidic “organ-on-a-chip” devices have been developed with the aim to replicate human tissues in vitro. However, there is no option to quantitatively monitor biological processes that take place within the chip, over time. Destructive methods in order to analyze, tissue formation, gene expression, protein secretion etc. require the harvest of the “tissue” at a certain time point. Described herein are methods and compositions for non-destructive molecular imaging methods and systems in order to quantitatively monitor specific biological processes, over time, within the chip, without the need to harvest.
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公开(公告)号：US20180217149A1
公开(公告)日：2018-08-02
申请号：US15747888
申请日：2016-07-28
Inventor： Cédric BOUZIGUES , Mouna ABDESSELEM , Antigoni ALEXANDROU , Thierry GACOIN , Jean-Pierre BOILOT
CPC classification number: G01N33/582 , G01N21/6428 , G01N21/6458 , G01N21/6489 , G01N33/5038 , G01N33/5091 , G01N2458/30 , G01N2458/40 , G01N2800/7009
Abstract: The invention relates to a method which can be used to analyse oxidising species in a sample, comprising the steps which consist of: placing (12) the sample in contact with a first photoluminescent agent, and a second optically active agent, at least the first photoluminescent agent including nanoparticles doped with rare earth elements and being oxidisable, the luminescence of the first photoluminescent agent varying, at least at one first wavelength, with the amount of oxidising species, and the signal emitted by the second optically active agent being constant or varying, at least at one second wavelength other than the first wavelength, with the amount of oxidising species in a direction opposite to the luminescence of the first photoluminescent agent; energising (16) the first and second agents in the sample; measuring (18) the light intensity of the sample at least at said first and second wavelengths; and assessing the presence and/or the amount of oxidising species by interpreting (20) said measured light intensities.
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8.发明授权
公开(公告)号：US09921212B2
公开(公告)日：2018-03-20
申请号：US14879507
申请日：2015-10-09
Applicant: KYOTO UNIVERSITY
Inventor： Junya Toguchida , Ryuta Nishikomori , Koji Yokoyama , Makoto Ikeya , Toshio Heike
IPC: G01N33/50 , C07D473/34
CPC classification number: G01N33/5041 , C07D473/34 , G01N33/5038 , G01N33/5044 , G01N2333/4703 , G01N2400/00 , G01N2400/40 , G01N2440/14 , G01N2500/10
Abstract: A method of screening for a therapeutic and/or preventive drug for cartilaginous hyperplasia and a therapeutic and/or preventive drug for cartilaginous hyperplasia are provided.The following are provided: a method of screening for a therapeutic and/or preventive drug for cartilaginous hyperplasia, comprising a step of culturing chondroprogenitor cells under conditions in which the cells are brought into contact with a test substance and conditions in which the cells are not brought into contact with the test substance and a step of determining the SOX9 promoter activity, cAMP level, or degree of phosphorylation of CREB in the cells or the extracellular matrix volume in a culture; and a therapeutic and/or preventive drug for cartilaginous hyperplasia, comprising as an active ingredient an adenylate cyclase inhibitor.
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公开(公告)号：US20170307592A1
公开(公告)日：2017-10-26
申请号：US15589302
申请日：2017-05-08
Inventor： Steven L. McKnight , Joseph M. Ready , Andrew A. Pieper , Gelin Wang , Ting Han
CPC classification number: G01N33/5058 , A61K38/00 , C12Q1/48 , G01N33/5014 , G01N33/5038 , G01N2333/91142
Abstract: Provided herein are methods for identifying a compound having cell-protective (e.g., neuroprotective) activity. Compounds identified therefrom are also provided. These compounds can be used to treat various diseases, disorders, or conditions associated with, for example, unwanted cell death.
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公开(公告)号：US20170307591A1
公开(公告)日：2017-10-26
申请号：US15516956
申请日：2015-10-29
Applicant: CHILDREN'S MEDICAL CENTER CORPORATION
Inventor： Clifford J. WOOLF , Alexandra S. LATINI , Nick A. ANDREWS , Alban LATREMOLIERE , Michael COSTIGAN
IPC: G01N33/50 , A61K31/519 , A61K31/4045 , G01N33/487
CPC classification number: G01N33/5038 , A61K31/4045 , A61K31/519 , C12Q1/26 , G01N33/487 , G01N33/5008 , G01N2560/00 , G01N2800/52
Abstract: Described herein are methods and assays relating to the inhibition of sepiapterin reductase and measuring said inhibition by measuring the level of sepiapterin. In some embodiments, the methods can further relate to treating a subject with BH4 and/or BH2.
