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公开(公告)号：US12042576B2
公开(公告)日：2024-07-23
申请号：US18116472
申请日：2023-03-02
Applicant: NATIONAL DEFENSE MEDICAL CENTER
Inventor： Chung-Hsing Chou , Dueng-Yuan Hueng , Tsung-Neng Tsai
CPC classification number: A61L27/3891 , A61K35/30 , A61K35/44 , A61L27/24 , A61L27/3633 , A61L27/3675 , A61L27/3808 , A61L27/383 , A61L27/3878 , C12N5/0618 , C12N5/0697 , A61L2430/32 , C12N2500/25 , C12N2500/46 , C12N2501/11 , C12N2501/115 , C12N2501/392 , C12N2502/088 , C12N2502/28 , C12N2533/54 , A61K35/30 , A61K2300/00 , A61K35/44 , A61K2300/00
Abstract: The invention relates to a cell sheet construct for neurovascular reconstruction. The cell sheet construct has a vascular endothelial cell layer and a neural stem cell layer, and the two layers are physically in direct contact with each other, where the vascular endothelial cell layer forms branching vasculatures, and the neural stem cell layer differentiates into neurons. The invention also relates to a method for manufacturing the cell sheet construct, having the following steps: culturing vascular endothelial cells on a substrate to form a vascular endothelial cell layer, seeding neural stem cells on the vascular endothelial cell layer to make the neural stem cells be physically in direct contact with the vascular endothelial cell layer, and culturing the neural stem cells and the vascular endothelial cell layer to differentiate into neurons and branching vasculatures to form a cell sheet construct.
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公开(公告)号：US10537664B2
公开(公告)日：2020-01-21
申请号：US14826927
申请日：2015-08-14
Applicant: National Defense Medical Center
Inventor： Chung-Hsing Chou , Dueng-Yuan Hueng , Tsung-Neng Tsai
Abstract: The invention relates to a cell sheet construct for neurovascular reconstruction. The cell sheet construct has a vascular endothelial cell layer and a neural stem cell layer, and the two layers are physically in direct contact with each other, where the vascular endothelial cell layer forms branching vasculatures, and the neural stem cell layer differentiates into neurons. The invention also relates to a method for manufacturing the cell sheet construct, having the following steps: culturing vascular endothelial cells on a substrate to form a vascular endothelial cell layer, seeding neural stem cells on the vascular endothelial cell layer to make the neural stem cells be physically in direct contact with the vascular endothelial cell layer, and culturing the neural stem cells and the vascular endothelial cell layer to differentiate into neurons and branching vasculatures to form a cell sheet construct.
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公开(公告)号：US11623024B2
公开(公告)日：2023-04-11
申请号：US16705344
申请日：2019-12-06
Applicant: NATIONAL DEFENSE MEDICAL CENTER
Inventor： Chung-Hsing Chou , Dueng-Yuan Hueng , Tsung-Neng Tsai
Abstract: The invention relates to a cell sheet construct for neurovascular reconstruction. The cell sheet construct has a vascular endothelial cell layer and a neural stem cell layer, and the two layers are physically in direct contact with each other, where the vascular endothelial cell layer forms branching vasculatures, and the neural stem cell layer differentiates into neurons. The invention also relates to a method for manufacturing the cell sheet construct, having the following steps: culturing vascular endothelial cells on a substrate to form a vascular endothelial cell layer, seeding neural stem cells on the vascular endothelial cell layer to make the neural stem cells be physically in direct contact with the vascular endothelial cell layer, and culturing the neural stem cells and the vascular endothelial cell layer to differentiate into neurons and branching vasculatures to form a cell sheet construct.
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公开(公告)号：US11439730B2
公开(公告)日：2022-09-13
申请号：US16705314
申请日：2019-12-06
Applicant: NATIONAL DEFENSE MEDICAL CENTER
Inventor： Chung-Hsing Chou , Dueng-Yuan Hueng , Tsung-Neng Tsai
Abstract: The invention relates to a cell sheet construct for neurovascular reconstruction. The cell sheet construct has a vascular endothelial cell layer and a neural stem cell layer, and the two layers are physically in direct contact with each other, where the vascular endothelial cell layer forms branching vasculatures, and the neural stem cell layer differentiates into neurons. The invention also relates to a method for manufacturing the cell sheet construct, having the following steps: culturing vascular endothelial cells on a substrate to form a vascular endothelial cell layer, seeding neural stem cells on the vascular endothelial cell layer to make the neural stem cells be physically in direct contact with the vascular endothelial cell layer, and culturing the neural stem cells and the vascular endothelial cell layer to differentiate into neurons and branching vasculatures to form a cell sheet construct.
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