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公开(公告)号：US12042576B2
公开(公告)日：2024-07-23
申请号：US18116472
申请日：2023-03-02
Applicant: NATIONAL DEFENSE MEDICAL CENTER
Inventor： Chung-Hsing Chou , Dueng-Yuan Hueng , Tsung-Neng Tsai
CPC classification number: A61L27/3891 , A61K35/30 , A61K35/44 , A61L27/24 , A61L27/3633 , A61L27/3675 , A61L27/3808 , A61L27/383 , A61L27/3878 , C12N5/0618 , C12N5/0697 , A61L2430/32 , C12N2500/25 , C12N2500/46 , C12N2501/11 , C12N2501/115 , C12N2501/392 , C12N2502/088 , C12N2502/28 , C12N2533/54 , A61K35/30 , A61K2300/00 , A61K35/44 , A61K2300/00
Abstract: The invention relates to a cell sheet construct for neurovascular reconstruction. The cell sheet construct has a vascular endothelial cell layer and a neural stem cell layer, and the two layers are physically in direct contact with each other, where the vascular endothelial cell layer forms branching vasculatures, and the neural stem cell layer differentiates into neurons. The invention also relates to a method for manufacturing the cell sheet construct, having the following steps: culturing vascular endothelial cells on a substrate to form a vascular endothelial cell layer, seeding neural stem cells on the vascular endothelial cell layer to make the neural stem cells be physically in direct contact with the vascular endothelial cell layer, and culturing the neural stem cells and the vascular endothelial cell layer to differentiate into neurons and branching vasculatures to form a cell sheet construct.
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公开(公告)号：US11623024B2
公开(公告)日：2023-04-11
申请号：US16705344
申请日：2019-12-06
Applicant: NATIONAL DEFENSE MEDICAL CENTER
Inventor： Chung-Hsing Chou , Dueng-Yuan Hueng , Tsung-Neng Tsai
Abstract: The invention relates to a cell sheet construct for neurovascular reconstruction. The cell sheet construct has a vascular endothelial cell layer and a neural stem cell layer, and the two layers are physically in direct contact with each other, where the vascular endothelial cell layer forms branching vasculatures, and the neural stem cell layer differentiates into neurons. The invention also relates to a method for manufacturing the cell sheet construct, having the following steps: culturing vascular endothelial cells on a substrate to form a vascular endothelial cell layer, seeding neural stem cells on the vascular endothelial cell layer to make the neural stem cells be physically in direct contact with the vascular endothelial cell layer, and culturing the neural stem cells and the vascular endothelial cell layer to differentiate into neurons and branching vasculatures to form a cell sheet construct.
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Patent Agency Ranking
公开(公告)号：US11439730B2
公开(公告)日：2022-09-13
申请号：US16705314
申请日：2019-12-06
Applicant: NATIONAL DEFENSE MEDICAL CENTER
Inventor： Chung-Hsing Chou , Dueng-Yuan Hueng , Tsung-Neng Tsai
Abstract: The invention relates to a cell sheet construct for neurovascular reconstruction. The cell sheet construct has a vascular endothelial cell layer and a neural stem cell layer, and the two layers are physically in direct contact with each other, where the vascular endothelial cell layer forms branching vasculatures, and the neural stem cell layer differentiates into neurons. The invention also relates to a method for manufacturing the cell sheet construct, having the following steps: culturing vascular endothelial cells on a substrate to form a vascular endothelial cell layer, seeding neural stem cells on the vascular endothelial cell layer to make the neural stem cells be physically in direct contact with the vascular endothelial cell layer, and culturing the neural stem cells and the vascular endothelial cell layer to differentiate into neurons and branching vasculatures to form a cell sheet construct.
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公开(公告)号：US20230201423A1
公开(公告)日：2023-06-29
申请号：US18116472
申请日：2023-03-02
Applicant: NATIONAL DEFENSE MEDICAL CENTER
Inventor： Chung-Hsing CHOU , Dueng-Yuan Hueng , Tsung-Neng TSAI
CPC classification number: A61L27/3891 , A61L27/3633 , A61L27/383 , A61L27/3808 , A61L27/3878 , A61L27/3675 , A61L27/24 , C12N5/0697 , A61K35/30 , C12N5/0618 , A61K35/44 , A61L2430/32 , C12N2502/28 , C12N2502/088 , C12N2533/54 , C12N2500/25 , C12N2500/46 , C12N2501/392 , C12N2501/11 , C12N2501/115
Abstract: The invention relates to a cell sheet construct for neurovascular reconstruction. The cell sheet construct has a vascular endothelial cell layer and a neural stem cell layer, and the two layers are physically in direct contact with each other, where the vascular endothelial cell layer forms branching vasculatures, and the neural stem cell layer differentiates into neurons. The invention also relates to a method for manufacturing the cell sheet construct, having the following steps: culturing vascular endothelial cells on a substrate to form a vascular endothelial cell layer, seeding neural stem cells on the vascular endothelial cell layer to make the neural stem cells be physically in direct contact with the vascular endothelial cell layer, and culturing the neural stem cells and the vascular endothelial cell layer to differentiate into neurons and branching vasculatures to form a cell sheet construct.
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公开(公告)号：US10098966B2
公开(公告)日：2018-10-16
申请号：US15640529
申请日：2017-07-01
Applicant: National Defense Medical Center
Inventor： Teh-Min Hu , Dueng-Yuan Hueng , Jen-Kun Chen , Hsin-Ying Clair Chiou , Li-Hui Tsai
Abstract: The present invention relates to complex particles for delivering nitric oxide, method of producing the particles, and application of the particles, and more particularly, relates to complex particles for delivering nitric oxide and an additional therapeutic reagent.
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公开(公告)号：US10537664B2
公开(公告)日：2020-01-21
申请号：US14826927
申请日：2015-08-14
Applicant: National Defense Medical Center
Inventor： Chung-Hsing Chou , Dueng-Yuan Hueng , Tsung-Neng Tsai
Abstract: The invention relates to a cell sheet construct for neurovascular reconstruction. The cell sheet construct has a vascular endothelial cell layer and a neural stem cell layer, and the two layers are physically in direct contact with each other, where the vascular endothelial cell layer forms branching vasculatures, and the neural stem cell layer differentiates into neurons. The invention also relates to a method for manufacturing the cell sheet construct, having the following steps: culturing vascular endothelial cells on a substrate to form a vascular endothelial cell layer, seeding neural stem cells on the vascular endothelial cell layer to make the neural stem cells be physically in direct contact with the vascular endothelial cell layer, and culturing the neural stem cells and the vascular endothelial cell layer to differentiate into neurons and branching vasculatures to form a cell sheet construct.
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7.发明申请公开(公告)号：US20160045641A1
公开(公告)日：2016-02-18
申请号：US14826927
申请日：2015-08-14
Applicant: NATIONAL DEFENSE MEDICAL CENTER
Inventor： Chung-Hsing CHOU , Dueng-Yuan Hueng , Tsung-Neng TSAI
CPC classification number: A61L27/3891 , A61K35/30 , A61K35/44 , A61L27/24 , A61L27/3633 , A61L27/3675 , A61L27/3808 , A61L27/383 , A61L27/3878 , A61L2430/32 , C12N5/0618 , C12N5/0697 , C12N2500/25 , C12N2500/46 , C12N2501/11 , C12N2501/115 , C12N2501/392 , C12N2502/088 , C12N2502/28 , C12N2533/54 , A61K2300/00
Abstract: The invention relates to a cell sheet construct for neurovascular reconstruction. The cell sheet construct has a vascular endothelial cell layer and a neural stem cell layer, and the two layers are physically in direct contact with each other, where the vascular endothelial cell layer forms branching vasculatures, and the neural stem cell layer differentiates into neurons. The invention also relates to a method for manufacturing the cell sheet construct, having the following steps: culturing vascular endothelial cells on a substrate to form a vascular endothelial cell layer, seeding neural stem cells on the vascular endothelial cell layer to make the neural stem cells be physically in direct contact with the vascular endothelial cell layer, and culturing the neural stem cells and the vascular endothelial cell layer to differentiate into neurons and branching vasculatures to form a cell sheet construct.
Abstract translation: 本发明涉及用于神经血管重建的细胞片构建体。 细胞片构建体具有血管内皮细胞层和神经干细胞层，并且两层物理上彼此直接接触，其中血管内皮细胞层形成分支血管系统，神经干细胞层分化为神经元。 本发明还涉及一种用于制造细胞片构建体的方法，具有以下步骤：在基底上培养血管内皮细胞以形成血管内皮细胞层，在血管内皮细胞层上接种神经干细胞以制备神经干细胞 物理上与血管内皮细胞层直接接触，并培养神经干细胞和血管内皮细胞层，以分化为神经元和分支血管系统以形成细胞片构建体。
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