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    CELL CULTURE MEDIA COMPOSITION AND METHODS OF PRODUCING THEREOF
    2.
    发明申请
    CELL CULTURE MEDIA COMPOSITION AND METHODS OF PRODUCING THEREOF 审中-公开
    细胞培养基组合物及其生产方法

    公开(公告)号：US20160017277A1

    公开(公告)日：2016-01-21

    申请号：US14773299

    申请日：2014-02-17

    申请人： NEUCLONE BIOLOGICS PTY LTD

    发明人： Noelle Sunstrom

    IPC分类号： C12N5/00

    CPC分类号： C12N5/0037 ,  C12N5/0056 ,  C12N5/0682 ,  C12N2500/90 ,  C12N2501/305 ,  C12N2502/243 ,  C12N2502/99

    摘要： A serum free cell culture media, wherein the media is adapted to be conditioned by culturing a first set of eukaryotic cells in the media, wherein the first set of eukaryotic cells use an expression vector to excrete levels of desired complex proteins into the media; wherein said desired complex proteins include human Growth Hormone (hGH), Growth Hormone-like growth factors, insulin-like growth factors, insulin, modified insulins, cytokines, mitogenic proteases and mixtures thereof; and wherein the media is adapted to grow a set of eukaryotic cells.

    摘要翻译： 一种无血清细胞培养基，其中所述培养基适于通过在所述培养基中培养第一组真核细胞进行调节，其中所述第一组真核细胞使用表达载体将所需复合蛋白的水平排出到培养基中; 其中所述所需的复合蛋白包括人生长激素（hGH），生长激素样生长因子，胰岛素样生长因子，胰岛素，修饰的胰岛素，细胞因子，促有丝分裂蛋白酶及其混合物; 并且其中所述培养基适于生长一组真核细胞。

    SYNTHETIC ATTACHMENT MEDIUM FOR CELL CULTURE
    3.
    发明申请
    SYNTHETIC ATTACHMENT MEDIUM FOR CELL CULTURE 审中-公开
    细胞培养的合成附着介质

    公开(公告)号：US20150010999A1

    公开(公告)日：2015-01-08

    申请号：US14375614

    申请日：2013-01-31

    申请人： CORNING INCORPORATED

    发明人： Stephen Joseph Caracci ,  David Henry ,  Jessica Jo Kelley ,  Mark Alan Lewis ,  Yue Zhou

    IPC分类号： C12N5/00 ,  C12N5/0735 ,  B05D3/02 ,  C12N5/0775

    CPC分类号： C12N5/0068 ,  B05D3/0254 ,  C12M23/20 ,  C12N5/0037 ,  C12N5/0056 ,  C12N5/0606 ,  C12N5/0663 ,  C12N2500/90 ,  C12N2500/98 ,  C12N2533/30 ,  C12N2533/40 ,  C12N2533/50

    摘要： An aqueous cell culture medium composition includes an aqueous cell culture solution configured to support the culture of mammalian cells. The composition further includes a synthetic polymer conjugated to a polypeptide dissolved in the aqueous cell culture solution. The synthetic polymer conjugated to a polypeptide is configured to attach to the surface of a cell culture article under cell culture conditions. Incubation of the aqueous cell culture medium composition on a cell culture surface under cell culture conditions results is attachment to the surface of the synthetic polymer conjugated to the polypeptide.

    摘要翻译： 水性细胞培养基组合物包括配置成支持哺乳动物细胞培养的水性细胞培养液。 该组合物还包括与溶解在水性细胞培养溶液中的多肽缀合的合成聚合物。 与多肽缀合的合成聚合物被配置为在细胞培养条件下附着于细胞培养物品的表面。 水细胞培养基组合物在细胞培养条件下在细胞培养物表面上的孵育结果是与多肽缀合的合成聚合物表面的附着。

    MEDIA FOR CULTURING STEM CELLS
    5.
    发明申请
    MEDIA FOR CULTURING STEM CELLS 有权
    培养干细胞培养基

    公开(公告)号：US20130252329A1

    公开(公告)日：2013-09-26

    申请号：US13909128

    申请日：2013-06-04

    申请人： Technion Research & Development Foundation Limited

    发明人： Michal AMIT ,  Joseph Itskovitz-Eldor

    IPC分类号： C12N5/0735

    CPC分类号： C12N5/0606 ,  C12N5/0037 ,  C12N5/0043 ,  C12N5/0056 ,  C12N5/0602 ,  C12N5/0603 ,  C12N2500/90 ,  C12N2500/98 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/23 ,  C12N2501/2306 ,  C12N2506/02 ,  C12N2533/52

    摘要： Well-defined, xeno-free culture media which comprise a TGF-beta isoform or the chimera formed between IL6 and the soluble IL6 receptor (IL6RIL6), which are capable of mainataining stem cells, and particularly, human embryonic stem cells, in an undifferentiated state are provided. Also provided are cell cultures comprising the culture media and the stem cells and methods of expanding and deriving embryonic stem cells in such well-defined, xeno-free culture media. In addition, the present invention provides methods of differentiating ESCs or EBs formed therefrom for the generation of lineage specific cells.

    摘要翻译： 包含TGF-β同种型或在IL6和可溶性IL6受体（IL6RIL6）之间形成的嵌合体的定义良好的，不含异种的培养基，其能够将干细胞，特别是人类胚胎干细胞在未分化的 状态。 还提供了包含培养基和干细胞的细胞培养物以及在这种定义良好的不含异种植物的培养基中扩增和衍生胚胎干细胞的方法。 此外，本发明提供了区分由其形成的ESC或用于产生谱系特异性细胞的EBs的方法。