公开(公告)号：US20220296723A1

公开(公告)日：2022-09-22

申请号：US17806107

申请日：2022-06-09

申请人： Merck Sharp & Dohme LLC

发明人： Patrick McHugh ,  Michael Albert Winters ,  Janelle Konietzko

IPC分类号： A61K47/54 ,  C07H1/00 ,  A61K47/62

摘要： The present invention provides a number of process improvements related to the conjugation of capsular polysaccharides from Streptococcus pneumoniae to a carrier protein. These process are serotype specific and include acid hydrolysis, addition of sodium chloride to the reductive amination reaction, and addition of sucrose to dissolve polysaccharides. Polysaccharide-protein conjugates prepared using the processes of the invention can be included in multivalent pneumococcal conjugate vaccines.

公开(公告)号：US12060583B2

公开(公告)日：2024-08-13

申请号：US17046871

申请日：2019-04-19

申请人： Merck Sharp & Dohme LLC

发明人： Adam Kristopeit ,  Janelle Konietzko ,  Wanli Ma ,  Katherine Phillips ,  Andrew Swartz ,  Sheng-Ching Wang ,  Marc D. Wenger ,  Matthew Woodling ,  Tiago Matos

IPC分类号： B01D15/36 ,  A61K39/12 ,  A61K39/245 ,  C12N5/079 ,  C12N7/02

CPC分类号： C12N7/02 ,  B01D15/362 ,  B01D15/363 ,  C12N5/0621 ,  C12N2710/16111

摘要： The present invention relates to a scalable process for the purification of human cytomegalovirus particles from cell culture medium. In particular, the process involves a two step chromatography process starting with an anion exchange chromatography step followed by a polishing chromatography step selected from mixed mode chromatography or cation exchange chromatography.