公开(公告)号：US20240352424A1

公开(公告)日：2024-10-24

申请号：US18642228

申请日：2024-04-22

申请人： Board of Supervisors of Louisiana State University and Agricultural and Mechanical College

发明人： Mandi J. LOPEZ ,  Takashi TAGUCHI

IPC分类号： C12N5/077 ,  A61L27/36 ,  C12M1/00 ,  C12M1/42 ,  C12M3/00 ,  C12N5/00

CPC分类号： C12N5/066 ,  A61L27/3637 ,  C12M21/08 ,  C12M29/10 ,  C12M35/04 ,  C12N5/0068 ,  C12N2501/15 ,  C12N2506/1384 ,  C12N2513/00

摘要： The invention relates to a method of producing a tendon neotissue from a population of adipose-derived stem cells (ASCs) subjected to mechanical and biological stimulations in a bioreactor system. The tendon neotissues are effective as implants to treat tendon or ligament injury in a subject. The invention also relates to a customized bioreactor useful for producing a tri-dimensional engineered tissue.

公开(公告)号：US20240342055A1

公开(公告)日：2024-10-17

申请号：US18752794

申请日：2024-06-25

申请人： ALPHINITY USA, INC.

发明人： Michael C. Gagne

IPC分类号： A61J1/10 ,  A61J1/14 ,  B01F27/61 ,  B01F27/71 ,  B01F27/91 ,  B01F35/513 ,  B01F35/75 ,  B01F101/44 ,  C12M1/00 ,  C12M1/06 ,  C12M3/00 ,  F04B43/06 ,  F04D1/00 ,  F04D29/40

CPC分类号： A61J1/10 ,  B01F27/61 ,  B01F27/71 ,  B01F27/91 ,  B01F35/513 ,  B01F35/7544 ,  C12M23/14 ,  C12M23/46 ,  C12M27/02 ,  C12M29/10 ,  F04D1/00 ,  F04D29/40 ,  A61J1/1481 ,  B01F2101/44 ,  F04B43/06

摘要： A method of pumping fluid out of a bioprocess vessel includes providing a bioprocess vessel defining an interior volume and having a bottom surface containing an aperture or port therein for the passage of fluid and a pump head integrally formed with or bonded to the bottom surface of the bioprocess vessel or secured to the port, wherein the pump head further comprises an inlet and an outlet with the inlet in fluid communication with the interior volume, the pump head further including a plurality of diaphragms, each diaphragm associated with a check-valve, wherein sequential actuation of the plurality of diaphragms pumps fluid from the interior volume of the bioprocess vessel into the inlet and out of the outlet. The plurality of diaphragms are actuated with a motor secured to the pump head. In other embodiments, the pump head is secured to the bioprocess vessel with fasteners.

公开(公告)号：US12116559B2

公开(公告)日：2024-10-15

申请号：US17559366

申请日：2021-12-22

申请人： Trizell Ltd.

发明人： Piia Kristiina Valonen ,  Hanna P. Lesch ,  Eva Kristiina Rasanen ,  Tarja Hannele Tuunanen ,  Minna Kristiina Karhinen ,  Seppo Ylä-Herttuala

IPC分类号： C12M1/00 ,  C07K14/005 ,  C12M1/02 ,  C12M1/12 ,  C12M1/34 ,  C12N15/86 ,  C12P21/02

CPC分类号： C12M29/18 ,  C07K14/005 ,  C12M1/02 ,  C12M25/18 ,  C12M29/10 ,  C12M41/26 ,  C12M41/46 ,  C12N15/86 ,  C12P21/02 ,  C12N2740/15051

摘要： We have modified a commercially-available adherent cell culture bioreactor in several ways to increase productivity of cultured cells, while decreasing contamination risk. We found that modifying a commercially-available adherent cell culture bioreactor to provide for slower cell culture medium flow unexpectedly and dramatically increases the productivity of the cultured adherent cells. We also developed a new sampling manifold configuration and new way of taking samples, to reduce contamination risk.

公开(公告)号：US12110484B2

公开(公告)日：2024-10-08

申请号：US17837385

申请日：2022-06-10

申请人： STOBBE GMBH

发明人： Per Stobbe

IPC分类号： C12M1/00 ,  C12M1/06 ,  C12M1/12

CPC分类号： C12M47/10 ,  C12M23/28 ,  C12M23/38 ,  C12M23/40 ,  C12M25/02 ,  C12M27/02 ,  C12M29/00 ,  C12M29/04 ,  C12M29/10 ,  C12M29/18 ,  C12M29/26 ,  C12M37/04

摘要： Embodiments of the invention relate to a Disposable Bioprocess System including a Single-Use-Bioreactor, a Single-Use-Pump and a single-use micro-organism retention filter. Combined most suitable for cultivation of suspended micro-organisms in a liquid media at high micro-organism concentration in a perfusion mode continuous process for expression of biological material. The inlet port of the liquid Single-Use-Pump connects via a valve to the broth reservoir of the Single-Use-Bioreactor through a liquid conveying port. The outlet port of the liquid pumping Single-Use-Pump connects via a valve to a micro-organism retention filter. And a method for operating said sterile Disposable Bioprocess System in perfusion mode for continuously processing.

公开(公告)号：US20240287451A1

公开(公告)日：2024-08-29

申请号：US18656253

申请日：2024-05-06

申请人： Genzyme Corporation

发明人： Agata Villiger-Oberbek ,  Jianguo Yang ,  Yang Yang ,  Gabrielle Lorenzo

IPC分类号： C12N5/071 ,  C12M1/00 ,  C12M1/32 ,  C12M3/04 ,  C12P21/00

CPC分类号： C12N5/0602 ,  C12M23/12 ,  C12M27/10 ,  C12M29/10 ,  C12P21/00 ,  C12N2510/02 ,  C12N2527/00

摘要： Provided herein are methods of culturing a mammalian cell and various methods that utilize these culturing methods. Also provided are multi-well cell culture plates, e.g., for use in perfusion culturing methods.

公开(公告)号：US20240271072A1

公开(公告)日：2024-08-15

申请号：US18625358

申请日：2024-04-03

申请人： Battelle Memorial Institute

发明人： Charles L. Desanti ,  Ryan W. Daly ,  David Charles Seibert, JR. ,  Patrick Norris ,  John Bartholomew ,  Sherry K. Blight

IPC分类号： C12M3/06 ,  C12M1/00 ,  C12M1/34

CPC分类号： C12M23/16 ,  C12M23/34 ,  C12M23/40 ,  C12M27/18 ,  C12M29/10 ,  C12M41/46

摘要： A biological culture unit comprises a chamber body and a valve plate. The chamber body has defined therein a growth chamber and an aliquot chamber. The valve plate is disposed on the top surface of the chamber body and is movable to define selectable configurations of the biological culture unit for: (1) loading the growth chamber; (3) transferring an aliquot from the growth chamber to the aliquot chamber via a path defined within the biological culture unit in the transfer position; and (4) extracting the aliquot from the aliquot chamber. The selectable positions may further include (2) a growth position, and (5) a termination position for putting a termination agent into the growth and aliquot chambers. The valve plate may further include a neutralizer port that is aligned with the aliquot chamber in the loading position, for loading a neutralizing agent into the aliquot chamber.

公开(公告)号：US20240218306A1

公开(公告)日：2024-07-04

申请号：US18484728

申请日：2023-10-11

申请人： The Trustees of the University of Pennsylvania

发明人： Dongeun Huh ,  Mark Mondrinos ,  Woo Yul Byun

IPC分类号： C12M3/00 ,  A61L27/18 ,  A61L27/22 ,  A61L27/36 ,  A61L27/38 ,  A61L27/50 ,  A61L27/52 ,  C08L83/04 ,  C12M1/00 ,  C12M1/12 ,  C12M3/06 ,  C12N5/00 ,  G01N33/50 ,  G09B23/30

CPC分类号： C12M21/08 ,  A61L27/18 ,  A61L27/22 ,  A61L27/3633 ,  A61L27/3804 ,  A61L27/50 ,  A61L27/52 ,  C08L83/04 ,  C12M3/00 ,  C12M23/16 ,  C12M23/20 ,  C12M25/02 ,  C12M25/14 ,  C12M29/10 ,  C12N5/0018 ,  C12N5/0062 ,  C12N5/0068 ,  G01N33/5005 ,  G01N33/5044 ,  G09B23/30 ,  G09B23/306 ,  C12N2533/30 ,  C12N2533/54 ,  C12N2535/00 ,  C12N2537/10

摘要： The presently disclosed subject matter provides a biomimetic lung disease model, and methods of its production and use. In one exemplary embodiment, the biomimetic lung disease model can include a first and second microchannel with a membrane coated with airway epithelial cells disposed between the microchannels and at least one device coupled to the biomimetic model that delivers an agent to at least one microchannel. In certain embodiments, the agent is cigarette smoke.

公开(公告)号：US20240182829A1

公开(公告)日：2024-06-06

申请号：US18441108

申请日：2024-02-14

申请人： Axogen Corporation

发明人： Daniel BAROUSSE ,  Gerhard Andrew FOELSCHE

IPC分类号： C12M3/00 ,  B65D21/00 ,  C12M1/00 ,  C12M1/12 ,  C12M1/33

CPC分类号： C12M21/08 ,  C12M23/44 ,  C12M25/10 ,  C12M29/10 ,  C12M45/02 ,  C12M45/09 ,  C12M47/04 ,  B65D21/00

摘要： Tissue-processing containers are disclosed for facilitating multistep processing of tissue. Also disclosed are systems incorporating the tissue-processing containers that include shakers, incubators, controllers, and pumps. Multistep methods are disclosed for processing tissues using the tissue-processing containers. The tissue-processing containers are specially designed to achieve efficiencies in automated tissue processing, exposure of tissues to processing media, and multistep tissue processing protocols. The described tissue-processing containers comprise a cup-like cavity comprising a middle hole covered by a mesh screen, troughs for separate processing steps, and are stackable in a substantially airtight and substantially watertight manner. Tissues for processing using the described tissue-processing containers include nerve tissue. Also described herein is a transport housing for the transport of stacks of tissue-processing containers, and which may directly incubate those containers, or which may be placed inside a separate incubator for incubating the containers.

公开(公告)号：US20240174966A1

公开(公告)日：2024-05-30

申请号：US18277298

申请日：2022-02-25

申请人： SECURECELL AG

发明人： Carlo ANDRETTA

IPC分类号： C12M1/26 ,  C12M1/00 ,  C12M1/12 ,  C12M1/34 ,  C12M3/00

CPC分类号： C12M33/14 ,  C12M23/38 ,  C12M23/48 ,  C12M29/10 ,  C12M29/14 ,  C12M29/20 ,  C12M37/04 ,  C12M41/32

摘要： A fluid sampling system for biotechnological applications, comprises a bioreactor chamber (2) for a fluid culture medium (4) containing cells (6) and further comprising transfer means (8) for transferring controlled amounts of culture medium from said bioreactor chamber to a target container (10). The transfer means comprise: a perfusion probe (12) with a fluid-tight probe housing surrounding an internal probe volume (14) and having an inlet probe aperture (16) and an outlet probe aperture (18); a fluid filtering element (20) sealingly connected to the probe housing and forming a cover of the inlet probe aperture. A fluid connection line comprises a fluid receptacle (30) disposed between the outlet probe aperture (18) of the perfusion probe and the target container (10) and disposed on a weight measuring station (32) configured for acquisition of a weight signal corresponding to the fluid receptacle's momentary weight.

公开(公告)号：US20240166984A1

公开(公告)日：2024-05-23

申请号：US18283748

申请日：2022-03-23

申请人： JANSSEN BIOTECH, INC.

发明人： Caitlin O'MAHONY-BARTNETT ,  Barry J. MC CARTHY ,  Christopher W. RODE ,  Ronan HAYES ,  Fiona MADDEN ,  Jingjie MO ,  Francis C. MASLANKA ,  Kevin CLARK ,  Daniel A. TROUT ,  Pushpraj RANA ,  Emmanouela GRATSIA ,  Carl RAFFERTY ,  Karin M. BALSS ,  Olav LYNGBERG

IPC分类号： C12M1/36 ,  C12M1/00 ,  C12M1/34 ,  G16C20/20 ,  G16C20/70

CPC分类号： C12M41/48 ,  C12M29/10 ,  C12M41/26 ,  C12M41/32 ,  G16C20/20 ,  G16C20/70

摘要： Methods for determining glycation on a molecule and/or a glycan structure on a glycosylated molecule through the use of a combination of spectroscopic analysis and chemometric modeling are described. In addition, methods and systems for producing a molecule with a desired level of glycation, including a non-glycated molecule, and/or a desired level of a glycan structure on a glycosylated molecule are described.