公开(公告)号：US12077742B2

公开(公告)日：2024-09-03

申请号：US16853865

申请日：2020-04-21

申请人： Life Technologies Corporation

发明人： Nephi D. Jones ,  Christopher D. Brau

IPC分类号： C12M1/06 ,  B01F27/1111 ,  B01F27/191 ,  B01F27/2111 ,  B01F35/41 ,  B01F35/513 ,  B01F101/44 ,  C12M1/00 ,  C12M1/02 ,  C12N1/00

CPC分类号： C12M27/02 ,  B01F27/1111 ,  B01F27/191 ,  B01F27/2111 ,  B01F35/4121 ,  B01F35/513 ,  C12M1/007 ,  C12M1/02 ,  C12M23/14 ,  C12N1/00 ,  B01F2101/44

摘要： A method for mixing a biological suspension includes disposing a biological suspension within a compartment of a container, the biological suspension comprising cells or microorganisms suspended within a nutrient growth medium; and rotating a first drive line and laterally spaced apart second drive line within the compartment of the container so as to cause the drive lines to twist into a helical configuration and mix the biological suspension.

公开(公告)号：US11939561B2

公开(公告)日：2024-03-26

申请号：US16438815

申请日：2019-06-12

申请人： Sartorius Stedim Biotech GmbH

发明人： Diana Kronenberg

IPC分类号： B65B5/04 ,  B01F27/072 ,  B01F27/191 ,  B01F27/88 ,  B01F27/91 ,  B01F33/453 ,  B01F35/513 ,  B65B25/00 ,  B65D6/24 ,  B65D19/38 ,  B65D19/40 ,  B65D77/00 ,  B65D77/04 ,  C12M1/00 ,  C12M1/12 ,  B01F101/44

CPC分类号： C12M23/26 ,  B01F27/0723 ,  B01F27/0724 ,  B01F27/191 ,  B01F27/88 ,  B01F27/91 ,  B01F33/4531 ,  B01F35/513 ,  B65B5/04 ,  B65B25/00 ,  B65D11/1873 ,  B65D19/385 ,  B65D19/40 ,  B65D77/003 ,  B65D77/0466 ,  C12M23/14 ,  C12M23/28 ,  C12M25/06 ,  B01F2101/44 ,  B65D2519/00532

摘要： A packaging for a flexible container, in particular for a disposable bag for use in a bioreactor, including a bottom part for fixing a first portion of the container, a ceiling part for fixing an opposite second portion of the container, a receiving space which on a first side is defined by the bottom part and on an opposite second side is defined by the ceiling part, and struts arranged between the bottom part and the ceiling part, which fix a defined distance between the bottom part and the ceiling part.

公开(公告)号：US11654408B2

公开(公告)日：2023-05-23

申请号：US16421168

申请日：2019-05-23

申请人： LIFE TECHNOLOGIES CORPORATION

发明人： Trevor G. Williams

IPC分类号： B01F27/2111 ,  B01F35/513 ,  B01F35/41 ,  C12M1/02 ,  C12M1/00 ,  C12M1/10 ,  B01F27/07 ,  B01F27/2121 ,  B01F101/44

CPC分类号： B01F35/4111 ,  B01F27/071 ,  B01F27/2111 ,  B01F27/2121 ,  B01F35/513 ,  C12M1/007 ,  C12M1/02 ,  C12M1/10 ,  B01F2101/44

摘要： A method for operating a bioreactor includes passing a drive shaft into a first tubular connector and a second tubular connector projecting from the first tubular connector, the first tubular connector and the second tubular connector being at least partially disposed within a container, the first tubular connector being more flexible than the second tubular connector, the second tubular connector having a length that comprises at least 20% of a combined length of the first tubular connector and the second tubular connector. Rotating the drive shaft so as to rotate the first tubular connector and the second tubular connector within the container.

公开(公告)号：US20240342055A1

公开(公告)日：2024-10-17

申请号：US18752794

申请日：2024-06-25

申请人： ALPHINITY USA, INC.

发明人： Michael C. Gagne

IPC分类号： A61J1/10 ,  A61J1/14 ,  B01F27/61 ,  B01F27/71 ,  B01F27/91 ,  B01F35/513 ,  B01F35/75 ,  B01F101/44 ,  C12M1/00 ,  C12M1/06 ,  C12M3/00 ,  F04B43/06 ,  F04D1/00 ,  F04D29/40

CPC分类号： A61J1/10 ,  B01F27/61 ,  B01F27/71 ,  B01F27/91 ,  B01F35/513 ,  B01F35/7544 ,  C12M23/14 ,  C12M23/46 ,  C12M27/02 ,  C12M29/10 ,  F04D1/00 ,  F04D29/40 ,  A61J1/1481 ,  B01F2101/44 ,  F04B43/06

摘要： A method of pumping fluid out of a bioprocess vessel includes providing a bioprocess vessel defining an interior volume and having a bottom surface containing an aperture or port therein for the passage of fluid and a pump head integrally formed with or bonded to the bottom surface of the bioprocess vessel or secured to the port, wherein the pump head further comprises an inlet and an outlet with the inlet in fluid communication with the interior volume, the pump head further including a plurality of diaphragms, each diaphragm associated with a check-valve, wherein sequential actuation of the plurality of diaphragms pumps fluid from the interior volume of the bioprocess vessel into the inlet and out of the outlet. The plurality of diaphragms are actuated with a motor secured to the pump head. In other embodiments, the pump head is secured to the bioprocess vessel with fasteners.

公开(公告)号：US20240253000A1

公开(公告)日：2024-08-01

申请号：US18416692

申请日：2024-01-18

申请人： Levitronix GmbH

发明人： Thomas HOLENSTEIN ,  Nils SCHLEGEL ,  Florian WASSMER

IPC分类号： B01F35/32 ,  B01F27/808 ,  B01F27/90 ,  B01F33/453 ,  B01F35/511 ,  B01F35/513 ,  B01F101/22 ,  B01F101/44 ,  C12M1/00 ,  C12M1/06

CPC分类号： B01F35/3213 ,  B01F27/808 ,  B01F27/90 ,  B01F33/453 ,  B01F35/511 ,  B01F35/513 ,  C12M23/28 ,  C12M27/02 ,  B01F2101/22 ,  B01F2101/44 ,  B01F2215/0422

摘要： A mixing device for mixing at least two substances includes a single-use device and a reusable device. The single-use device includes an impeller, vanes, and a cup for receiving the rotor housing. A magnetically effective core is in the rotor housing. The reusable device includes a stator to contactlessly magnetically drive the impeller about the axis of rotation and magnetically levitate the impeller with respect to the stator. A plurality of stabilizing elements is on the impeller for stabilizing the impeller against tilting. Each stabilizing element is arranged between two vanes and extends from a radial inner edge to a radial outer edge with respect to the radial direction, and each radial outer edge has a distance from the axis of rotation which is smaller than half the outer diameter of the impeller.

公开(公告)号：US11739289B2

公开(公告)日：2023-08-29

申请号：US16854183

申请日：2020-04-21

申请人： Lonza Limited

发明人： Michael Mietzner ,  Richard Falk ,  Rajesh Beri ,  Mark Caswell

IPC分类号： B01F27/00 ,  C12M1/06 ,  B01F27/191 ,  B01F27/1125 ,  B01F101/44

CPC分类号： C12M27/02 ,  B01F27/1125 ,  B01F27/191 ,  B01F2101/44

摘要： An impeller is usable in a variable diameter bioreactor having multiple vessel sections of successively increasing or decreasing volume. The impeller includes an impeller blade extending along an impeller blade axis between first and second axial ends and having opposed impeller blade faces, an impeller blade leading edge, and an impeller blade trailing edge. Each of the leading and trailing edges defines a helix or spiral between the axial ends of the impeller blade. In certain arrangements, the impeller blade is one of at least two impeller blades joined together along an impeller shaft extending axially along the impeller blade axis mentioned, and the helix or spiral has a pitch approximately equal to one half of a length between the first and second axial ends.

公开(公告)号：US11591556B2

公开(公告)日：2023-02-28

申请号：US16863702

申请日：2020-04-30

申请人： LIFE TECHNOLOGIES CORPORATION ,  Takeda Pharmaceutical Company Limited

发明人： Kurt T. Kunas ,  Robert V. Oakley ,  Fauad F. Hasan ,  Michael E. Goodwin ,  Jeremy K. Larsen ,  Nephi D. Jones

IPC分类号： C12M1/00 ,  B01F27/07 ,  B01F27/88 ,  B01F27/91 ,  B01F33/00 ,  B01F35/51 ,  B01F35/513 ,  C12M1/06 ,  C12M1/12 ,  B01F101/44

摘要： A reactor system includes a support housing having an interior surface bounding a chamber, the chamber having a vertically extending central longitudinal axis. A flexible bag is disposed within the chamber of the support housing and has an interior surface bounding a compartment. A mixing element is disposed within the compartment of the flexible bag. A drive element, such as a drive shaft, is secured the mixing element, wherein the mixing element is laterally offset from and/or is angled relative to the vertically extending central longitudinal axis of the support housing.

公开(公告)号：US11584910B2

公开(公告)日：2023-02-21

申请号：US16238591

申请日：2019-01-03

申请人： LIFE TECHNOLOGIES CORPORATION

发明人： Christopher Brau ,  Nephi Jones ,  Mark Smith

IPC分类号： B01F23/00 ,  B01F27/00 ,  C12M1/42 ,  C12M1/00 ,  C12N5/071 ,  C12M1/06 ,  B01F23/233 ,  B01F27/054 ,  B01F27/92 ,  B01F27/114 ,  B01F27/213 ,  B01F35/43 ,  B01F35/513 ,  B01F35/32 ,  B01F35/41 ,  C12M1/36 ,  B01F27/90 ,  B01F35/30 ,  B01F101/44

摘要： The present set of embodiments relate to a bioproduction system, method, and apparatus for creating a scalable bioreactor system. Specifically, the present set of embodiments enable the determination of bioreaction performance characteristics of a commercial scale by matching operational parameters between a small test scale bioreaction to that of a commercial scale bioreaction. The system and methods do not rely on simply making bioreactor apparatuses across scales the same dimensionally which would not account for differences in fluid dynamic properties between very small to very large volumes, but requires tuning of a variety of systems (mixing assembly, sparger system, and headspace airflow system) in conjunction with one another to achieve predictive outcomes.

公开(公告)号：US11471840B2

公开(公告)日：2022-10-18

申请号：US16718822

申请日：2019-12-18

申请人： Billups-Rothenberg, Inc.

发明人： Royal Q. Le ,  Kyong Son ,  Barry E. Rothenberg

IPC分类号： B01F23/00 ,  B01F23/10 ,  C12M1/34 ,  B01F25/4314 ,  B01F35/21 ,  B01F35/221 ,  B01F101/44

摘要： Provided herein are gas mixing systems, comprising a gas inlet for receiving two or more gases and a mixing chamber with a static mixer for mixing the gases. Preferred mixing chambers further comprise a reduced pressure compartment downstream of the static mixer that is in fluid communication with the gas inlet. A gas outlet is in fluid communication with the mixing chamber, and one or more sensors are coupled to the reduced pressure compartment and are configured to continuously sense various parameters such as barometric pressure and the percentage of oxygen in the gas mixture moving through the mixing device. Most typically, the readings of the sensor are pre-compensated for temperature, pressure, and humidity. Also provided herein are methods for using the same.

公开(公告)号：US20240350991A1

公开(公告)日：2024-10-24

申请号：US18687434

申请日：2022-02-18

申请人： SHIMADZU CORPORATION

发明人： Tomohisa HASUNUMA

IPC分类号： B01F33/453 ,  B01F35/00 ,  B01F35/30 ,  B01F101/44 ,  C12M1/00 ,  C12M1/06

CPC分类号： B01F33/4532 ,  B01F35/55 ,  C12M23/40 ,  C12M27/02 ,  C12M27/20 ,  B01F2035/352 ,  B01F2101/44

摘要： An agitator comprises: a bearing unit that holds a shaft unit of the agitator and slides rotatably with respect to the shaft unit; and a rotary blade unit that is fixed to the bearing unit and rotates together with the bearing unit. The bearing unit comprises a first member composed of a material and the rotary blade unit comprises a second member composed of a material different from that of the first member.