Integrated microfluidic system for generation of MicroOrganoSpheres (MOS)

    公开(公告)号:US11999934B2

    公开(公告)日:2024-06-04

    申请号:US18378921

    申请日:2023-10-11

    申请人: Xilis, Inc.

    摘要: A microfluidic apparatus includes a microfluidic chip for MicroOrganoSpheres (MOS) generation. A first channel is defined in a surface of the microfluidic chip and includes: a droplet generation portion including an inlet portion, a junction between the inlet portion and an emulsifying fluid channel, and a chamber downstream of the junction. A cross-sectional area of the chamber is larger than that of the inlet portion. The first channel includes a polymerization portion downstream of the droplet generation portion, the polymerization portion having a serpentine configuration. The apparatus includes a cartridge for MOS demulsification, including: a collection container; a substrate disposed on the collection container, and a membrane disposed between the collection container and the surface of the substrate. A second channel is defined in the surface of the substrate that faces the collection container and is fluidically connected to an output of the polymerization portion of the first channel.

    Biological culture unit
    7.
    发明授权

    公开(公告)号:US11976262B2

    公开(公告)日:2024-05-07

    申请号:US17002940

    申请日:2020-08-26

    IPC分类号: C12M1/00 C12M1/34 C12M3/06

    摘要: A biological culture unit comprises a chamber body and a valve plate. The chamber body has defined therein a growth chamber and an aliquot chamber. The valve plate is disposed on the top surface of the chamber body and is movable to define selectable configurations of the biological culture unit for: (1) loading the growth chamber; (3) transferring an aliquot from the growth chamber to the aliquot chamber via a path defined within the biological culture unit in the transfer position; and (4) extracting the aliquot from the aliquot chamber. The selectable positions may further include (2) a growth position, and (5) a termination position for putting a termination agent into the growth and aliquot chambers. The valve plate may further include a neutralizer port that is aligned with the aliquot chamber in the loading position, for loading a neutralizing agent into the aliquot chamber.

    Overpressure protection means
    10.
    发明授权

    公开(公告)号:US11952566B2

    公开(公告)日:2024-04-09

    申请号:US17052942

    申请日:2019-01-04

    摘要: The invention relates to an apparatus 10 for storing and/or processing at least one medium 11, in particular a bioprocessing device, comprising at least one disposable container 1, which is designed to accommodate at least some of the at least one medium 11, and at least one overpressure protection means 5, which is fluid-connected to the at least one disposable container 1. The at least one overpressure protection means is designed, when triggered, to conduct at least some of the at least one medium 11 into an apparatus region 3a′ in front of the overpressure protection means 5 in relation to a flow direction F, in particular into the at least one disposable container 1 and/or into at least one further, second container 1a.