公开(公告)号：US11905509B2

公开(公告)日：2024-02-20

申请号：US17512558

申请日：2021-10-27

Applicant: Koligo Therapeutics, Inc

Inventor： Rolf Wolters ,  Jules Valenti ,  Tom Gurski

IPC: C12M1/00 ,  A61K35/12 ,  B04B1/02 ,  C12M1/26 ,  B04B1/00

CPC classification number: C12M47/04 ,  A61K35/12 ,  B04B1/00 ,  B04B1/02 ,  C12M33/10 ,  C12M45/05 ,  C12M45/06 ,  C12M45/09

Abstract: Cell separation systems and methods of separating cells are disclosed. In an embodiment, a cell separation system is described that comprises a non-transitory storage device that executes a centrifugation program to separate cell volume from biologic material volume; a heating mechanism; a containment mechanism; and an assembly comprised of a single-walled centrifugation bowl. In an embodiment, methods of separating cells are disclosed whereby cells are separated by agitating a volume of biologic material and a volume digestion media to form a digested volume of biologic material; centrifuging the digested volume of biologic material; removing a portion of a resulting waste via at least one fluid outlet; isolating a different portion of the waste, and removing the concentrated cell volumes from the reservoir.

公开(公告)号：US20230203552A1

公开(公告)日：2023-06-29

申请号：US18177995

申请日：2023-03-03

Applicant: Fluid Quip Process Technologies, LLC

Inventor： Neal Jakel ,  John Kwik ,  Michael Franko ,  Andrew Whalen

IPC: C12P19/02 ,  C12P19/14 ,  C12M1/00 ,  C13K1/06 ,  C12P7/10 ,  C12P7/649 ,  C12P7/06 ,  C12M1/33

CPC classification number: C12P19/02 ,  C12P19/14 ,  C12M21/12 ,  C12M47/10 ,  C12M23/58 ,  C12M45/09 ,  C13K1/06 ,  C12P7/10 ,  C12P7/649 ,  C12P7/06 ,  C12M29/04 ,  C12M45/02 ,  C12P2203/00 ,  Y02E50/10 ,  Y02E50/30

Abstract: An improved dry grind system and method for producing a sugar stream from grains or similar carbohydrate sources and/or residues, such as for biofuel production. In particular, a sugar/carbohydrate stream, which includes a desired Dextrose Equivalent (DE) where DE describes the degree of conversion of starch to dextrose (aka glucose) and/or has had removed therefrom an undesirable amount of unfermentable components, can be produced after saccharification and prior to fermentation (or other sugar conversion process), with such sugar stream being available for biofuel production, e.g., alcohol production, or other processes. In addition, the systems and methods also can involve the removal of certain grain components, e.g., corn kernel components, including protein, oil and/or fiber, prior to fermentation or other conversion systems. In other words, sugar stream production and/or grain component separation occurs on the front end of the system and method.

公开(公告)号：US11666605B2

公开(公告)日：2023-06-06

申请号：US17129642

申请日：2020-12-21

Applicant: GID BIO, Inc.

Inventor： William W. Cimino ,  Adam J. Katz ,  Ramon Llull

IPC: C12M1/06 ,  A61K35/35 ,  C12M1/00 ,  A61K45/06 ,  C12N5/077 ,  A61K35/12 ,  A61M1/00

CPC classification number: A61K35/35 ,  A61K35/12 ,  A61K45/06 ,  A61M1/60 ,  A61M1/892 ,  C12M27/02 ,  C12M45/09 ,  C12N5/0653 ,  A61M2202/08 ,  A61M2205/75

Abstract: Preparation of a product comprising stromal vascular fraction cells includes washing human biological material comprising adipose in a container apparatus having an internal filter, which divides an internal containment volume of the container apparatus into a tissue retention volume on one side of the filter and a filtrate volume on an opposite side of the filter, and a mixing device with at least one rotatable mixing member disposed in the tissue retention volume. The washing includes operation of the mixing device to rotate the mixing member through the human biological material within the tissue retention volume, and the washing is followed by digesting washed material within the internal containment volume with added enzyme, centrifuging of the container apparatus to prepare a centrifuged pellet in the filtrate volume, selectively removing material of the pellet and preparing a product with a mixture of stromal vascular fraction cells of removed pellet material and an aqueous suspension liquid.

公开(公告)号：US10011625B2

公开(公告)日：2018-07-03

申请号：US15141605

申请日：2016-04-28

Applicant: XYLECO, INC.

Inventor： Marshall Medoff ,  Thomas Craig Masterman

IPC: C12M1/00 ,  C07G1/00 ,  C08H8/00 ,  C10L1/02 ,  C12P7/10 ,  C12P7/40 ,  C12P7/62 ,  C12P19/14 ,  C12P7/02 ,  C12M1/26 ,  C12M1/42 ,  C12M1/34 ,  C08B37/00 ,  C12P7/54 ,  C13K1/02 ,  C12M1/33 ,  C12P19/04

CPC classification number: C07G1/00 ,  C08B37/0003 ,  C08H8/00 ,  C10G2300/1014 ,  C10L1/02 ,  C10L1/026 ,  C12M21/12 ,  C12M29/00 ,  C12M33/00 ,  C12M35/02 ,  C12M41/00 ,  C12M41/32 ,  C12M41/36 ,  C12M45/02 ,  C12M45/06 ,  C12M45/07 ,  C12M45/09 ,  C12P7/02 ,  C12P7/10 ,  C12P7/40 ,  C12P7/54 ,  C12P7/62 ,  C12P19/04 ,  C12P19/14 ,  C12P2201/00 ,  C12P2203/00 ,  C13K1/02 ,  Y02E50/13 ,  Y02E50/16 ,  Y02E50/17 ,  Y02E50/30 ,  Y02P30/20 ,  Y02T50/678

Abstract: Biomass feedstocks (e.g., plant biomass, animal biomass, and municipal waste biomass) are processed to produce useful products, such as fuels. For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, to produce a product or intermediate, e.g., energy, a food, a fuel, or a material.

公开(公告)号：US09982283B2

公开(公告)日：2018-05-29

申请号：US14411473

申请日：2012-07-11

Applicant: Minoru Genta ,  Seiichi Terakura ,  Ryosuke Uehara ,  Seiji Kobayashi

Inventor： Minoru Genta ,  Seiichi Terakura ,  Ryosuke Uehara ,  Seiji Kobayashi

IPC: C12P19/14 ,  C13K1/02 ,  C12P7/10 ,  C13B20/16 ,  C12M1/40 ,  C12M1/00 ,  C12M1/26 ,  C12P7/02 ,  C12P19/02

CPC classification number: C12P19/14 ,  B01J2219/00006 ,  B01J2219/0004 ,  C12M21/18 ,  C12M29/00 ,  C12M29/14 ,  C12M33/00 ,  C12M45/06 ,  C12M45/09 ,  C12M45/20 ,  C12M47/10 ,  C12P7/02 ,  C12P7/10 ,  C12P19/02 ,  C12P2201/00 ,  C12P2203/00 ,  C13B20/165 ,  C13K1/02 ,  Y02E50/16

Abstract: Provided is a biomass hydrothermal decomposition system which includes: a biomass supply unit that supplies a biomass raw material; hydrothermally decomposing the biomass raw material using pressurized hot water; a hydrothermal decomposition unit that dissolves lignin components and hemicellulose components in the pressurized hot water; a biomass solid fraction discharge unit that discharges a biomass solid fraction from the hydrothermal decomposition unit; an enzymatic liquefaction tank that is in connection with the biomass solid fraction discharge unit, and in which the discharged biomass solid fraction is introduced, and an enzyme is supplied to the biomass solid fraction to liquefy the biomass solid fraction; and a discharge unit that discharges the liquefied biomass solid fraction.

公开(公告)号：US09907883B2

公开(公告)日：2018-03-06

申请号：US14440846

申请日：2014-01-10

Applicant: THE GID GROUP, INC.

Inventor： Ramon Llull ,  Severiano Dos Anjos Vilaboa ,  William W. Cimino

IPC: A61L27/36 ,  A61K35/32 ,  A61L27/20 ,  A61L27/38 ,  A61L27/54 ,  C12M1/00 ,  C12N5/071 ,  A61L27/22 ,  C12N5/0775

CPC classification number: A61L27/3683 ,  A61K35/32 ,  A61L27/20 ,  A61L27/225 ,  A61L27/3608 ,  A61L27/3616 ,  A61L27/365 ,  A61L27/3804 ,  A61L27/3821 ,  A61L27/3847 ,  A61L27/54 ,  A61L2300/414 ,  A61L2430/02 ,  C12M45/09 ,  C12N5/0663 ,  C12N2509/00 ,  C08L5/08

Abstract: Cancellous bone material may be processed in a portable container apparatus to prepare a stromal vascular fraction concentrate. Cancellous bone material may be washed to remove non-bone material, digested and digested material centrifuged, with all operations being performed on cancellous bone material while disposed in the portable container apparatus. Uncultured stromal vascular fraction cells separated from enzyme-digested cancellous bone material, and which contain osteoblasts and osteoclasts, may be removed from the portable container and used without culturing for a variety of medical applications. Medical treatment compositions may be prepared including recovered stromal vascular fraction cells and scaffold material.

公开(公告)号：US20180037863A1

公开(公告)日：2018-02-08

申请号：US15550582

申请日：2016-03-16

Applicant: Iogen Corporation

Inventor： Brian Foody ,  Daniel G. MacDonald ,  Kristin Martens ,  Natacha Leduc ,  John Dechman ,  Robert Griffin

IPC: C12M1/00 ,  C12P19/14 ,  D21C3/06 ,  C12P7/14 ,  D21C1/02 ,  D21C1/04 ,  C12P19/02 ,  C12P7/10

CPC classification number: C12M45/03 ,  B01D53/002 ,  B01D53/1481 ,  B01D53/501 ,  B01D2251/60 ,  B01D2252/10 ,  B01D2252/102 ,  B01D2257/302 ,  C12M45/09 ,  C12M45/20 ,  C12P7/10 ,  C12P7/14 ,  C12P19/02 ,  C12P19/14 ,  C12P2201/00 ,  C12P2203/00 ,  C12Y302/01004 ,  D21C1/02 ,  D21C1/04 ,  D21C3/06 ,  Y02E50/16

Abstract: A process for producing ethanol from lignocellulosic biomass includes adding at least one of sulfur dioxide and sulfurous acid to the lignocellulosic biomass to provide an equivalent sulfur dioxide loading of at least 10 wt % sulfur dioxide to dry lignocellulosic biomass. The acidified lignocellulosic biomass is pretreated at a temperature above about 185° C. and for a pretreatment time less than about 10 minutes, to provide a pretreated biomass composition wherein the biomass is readily hydrolyzed by enzymes. Advantageously, sulfur dioxide from at least one of the flash stream and a stream derived from the flash is recovered and recycled back into the process.

公开(公告)号：US20170349923A1

公开(公告)日：2017-12-07

申请号：US15538850

申请日：2015-12-22

Applicant: Iogen Energy Corporation

Inventor： Torbjorn van der Meulen ,  Lee Castiglione ,  Stephanie Lepine

IPC: C12P19/14 ,  C12P7/14 ,  C12M1/00 ,  C12M1/06 ,  C12P19/02 ,  C12M1/40

CPC classification number: C12P19/14 ,  C12M21/12 ,  C12M21/18 ,  C12M27/02 ,  C12M45/09 ,  C12P7/10 ,  C12P7/14 ,  C12P19/02 ,  C12Y302/01004 ,  C12Y302/01021 ,  Y02E50/16

Abstract: Provided herein is a process for hydrolyzing a cellulosic feedstock to produce sugar. The process comprises introducing a pretreated cellulosic slurry to an inlet region of a plug flow hydrolysis reactor using a slurry introduction device that reduces the axial momentum of the slurry at the surface of the reactor contents. The cellulosic feedstock slurry is hydrolyzed in the plug flow hydrolysis reactor by contacting the cellulosic feedstock with at least cellulase enzymes to produce glucose. Also provided herein is a vertically-oriented, unmixed downflow hydrolysis reactor for hydrolyzing a pretreated cellulosic feedstock slurry which comprises such a slurry introduction device disposed in a top region thereof.

公开(公告)号：US09580763B2

公开(公告)日：2017-02-28

申请号：US14759108

申请日：2014-01-21

Applicant: SEKAB E-TECHNOLOGY AB

Inventor： Marie-Louise Wallberg ,  Elias Sundvall

IPC: G06K9/00 ,  C12Q3/00 ,  C12M1/34 ,  C12M1/40 ,  C12M1/36 ,  C12M1/00 ,  C12P19/02 ,  C12P19/14 ,  C12P7/10 ,  D21C1/00 ,  D21C5/00

CPC classification number: C12Q3/00 ,  C12M21/18 ,  C12M41/32 ,  C12M41/36 ,  C12M41/48 ,  C12M45/09 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  D21C1/00 ,  D21C5/005 ,  Y02E50/16

Abstract: There is provided a method of producing a hydrolysate containing sugar from a lingocellulosic biomass, comprising the steps of: a) pretreating the lingocellulosic biomass in a pretreatment process to form a slurry comprising cellulosic particles; b) saccharification of the slurry obtained in step a) in the presence of hydrolytic enzymes in an enzymatic hydrolysis process to obtain a hydrolysate containing sugar; c) analyzing cellulosic particles present in the slurry obtained in step a) and/or cellulosic particles present in the enzymatic hydrolysis process during step b) using an image analysis method to obtain a data set; and d) controlling at least one process parameter of the pretreatment process in step a) and/or enzymatic hydrolysis process in step b) in response to the data set obtained in step c). A corresponding system is also provided.

Abstract translation: 提供了从纤维素生物质生产含糖的水解产物的方法，包括以下步骤：a）在预处理过程中预处理纤维素生物质以形成包含纤维素颗粒的浆料; b）在酶水解过程中在水解酶存在下，在步骤a）中获得的浆料糖化，得到含糖的水解产物; c）分析存在于步骤a）中获得的浆料中的纤维素颗粒和/或在步骤b）期间存在于酶水解过程中的纤维素颗粒使用图像分析方法获得数据集; 以及d）响应于在步骤c）中获得的数据集，在步骤a）和/或步骤b）中控制预处理过程的至少一个工艺参数和/或酶水解过程。 还提供了相应的系统。

公开(公告)号：US09574212B2

公开(公告)日：2017-02-21

申请号：US15199638

申请日：2016-06-30

Applicant: Iogen Corporation

Inventor： Brian Foody ,  Jeffrey S. Tolan ,  Daniel MacDonald ,  Patrick J. Foody

IPC: C12P7/14 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12M1/00 ,  D21C1/02 ,  D21C1/04 ,  B01D53/00

CPC classification number: C12M45/03 ,  B01D53/002 ,  B01D2257/302 ,  C12M45/09 ,  C12M45/20 ,  C12P7/10 ,  C12P7/14 ,  C12P19/02 ,  C12P19/14 ,  C12P2201/00 ,  C12Y302/01004 ,  D21C1/02 ,  D21C1/04 ,  D21C3/06 ,  Y02E50/16

Abstract: The disclosure provides a process for producing a fermentation product from a lignocellulosic feedstock. The process describes soaking a lignocellulosic feedstock in an aqueous solution to produce a soaked feedstock. The soaked feedstock is at least partially dewatered and the at least partially dewatered feedstock is subjected to pretreating in the presence of sulfur dioxide, sulfurous acid or a combination thereof to produce a pretreated feedstock composition. The pretreated feedstock composition is fed to an enzymatic hydrolysis in which the concentration of dissolved solids fed to the enzymatic hydrolysis is at least 50% (w/w) of the concentration of dissolved solids in the pretreated feedstock composition. The cellulose in the pretreated feedstock composition is hydrolyzed with cellulase enzymes in the presence of the dissolved solids to produce glucose. The glucose is fermented to produce the fermentation product.

Abstract translation: 本公开提供了从木质纤维素原料生产发酵产物的方法。 该方法描述了将木质纤维素原料浸泡在水溶液中以产生浸渍的原料。 浸渍的原料至少部分脱水，并且在二氧化硫，亚硫酸或其组合的存在下对至少部分脱水的原料进行预处理以产生预处理的原料组合物。 将预处理的原料组合物加入酶水解，其中进料到酶水解的溶解固体的浓度为预处理的原料组合物中溶解固体浓度的至少50％（w / w）。 预处理的原料组合物中的纤维素在溶解的固体存在下用纤维素酶水解以产生葡萄糖。 葡萄糖被发酵以产生发酵产物。