公开(公告)号：US12129458B2

公开(公告)日：2024-10-29

申请号：US17126482

申请日：2020-12-18

Applicant: Sartorius Stedim Biotech GmbH

Inventor： Christian Manzke

IPC: B01D63/08 ,  B01D65/02 ,  C12M1/00 ,  C12M1/34

CPC classification number: C12M29/04 ,  B01D63/081 ,  B01D63/082 ,  B01D63/084 ,  B01D65/022 ,  C12M23/40 ,  C12M41/00 ,  B01D2313/105 ,  B01D2313/18 ,  B01D2313/243 ,  B01D2321/346

Abstract: A filter system (100) for biopharmaceutical processes includes: at least one filter unit (10); and at least one distributor plate (200), against which the filter unit rests. A tubeless distributor unit (202) is arranged within the distributor plate and is fluidically connected to the filter unit. The distributor unit guides the fluid to be filtered to the filter unit and/or receives and discharges the filtered fluid from the filter unit. The distributor unit includes at least one active control element (232; 238), with which a fluid flow (14) through the distributor unit) and the filter unit is controlled in an open loop flow or a closed loop flow. The fluid connection between the filter unit and the distributor plate is tubeless. Also provided are a distributor plate (200) for a filter system (100) for biopharmaceutical processes and a method for producing a filter system (100) for biopharmaceutical processes.

公开(公告)号：US20240287428A1

公开(公告)日：2024-08-29

申请号：US18654917

申请日：2024-05-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Michael E. Goodwin ,  Nephi D. JONES

IPC: C12M1/00 ,  B01F27/07 ,  B01F27/113 ,  B01F27/2121 ,  B01F27/213 ,  B01F27/231 ,  B01F27/61 ,  B01F33/00 ,  B01F35/22 ,  B01F35/513 ,  B01F35/71 ,  C12M1/06 ,  C12M1/34 ,  C12M3/00

CPC classification number: C12M23/14 ,  B01F27/071 ,  B01F27/113 ,  B01F27/2121 ,  B01F27/213 ,  B01F27/2312 ,  B01F27/61 ,  B01F33/86 ,  B01F35/2209 ,  B01F35/513 ,  C12M23/26 ,  C12M23/48 ,  C12M23/52 ,  C12M27/02 ,  C12M27/06 ,  C12M41/00 ,  B01F35/715

Abstract: A fluid mixing system includes a support housing having an interior surface bounding a chamber. A flexible bag is disposed within the chamber of the support housing, the flexible bag having an interior surface bounding a compartment. An impeller is disposed within the chamber of the flexible bag. A drive shaft is coupled with the impeller such that rotation of the drive shaft facilitates rotation of the impeller. A drive motor assembly is coupled with the draft shaft and is adapted to rotate the drive shaft. An adjustable arm assembly is coupled with the drive motor assembly and is adapted to move the drive motor assembly which in turn moves the position of the drive shaft and impeller. An electrical controller can control movement of the adjustable arm.

公开(公告)号：US20240191170A1

公开(公告)日：2024-06-13

申请号：US18286397

申请日：2021-07-02

Applicant: Charlotte OHONIN

Inventor： Charlotte OHONIN ,  Ferenc SARKÖZI

IPC: C12M1/02 ,  C12M1/00 ,  C12M1/34

CPC classification number: C12M27/00 ,  C12M23/40 ,  C12M41/00

Abstract: The present invention relates to a cultivation device (10) for cultivating cell cultures, having



at least one aggregation layer (20) with at least one cell input (22) for introducing a cell suspension (ZS) and at least one aggregation input (24) for introducing an aggregating fluid (AF), wherein the cell input (22) and the aggregation input (24) lead into a mixing section (26) of the aggregation layer (20) for mixing the cell suspension (ZS) and the aggregating fluid (AF) in order to form cell aggregates (ZA),
further having at least one separating layer (30) with a separating input (32) for introducing the cell aggregates (ZA) from the mixing section (26), at least one separating section (36) for separating an individual cell aggregate (ZA) from the suspension and a separating output (34) for discharging the remaining suspension after the at least one separating section (36),
further having at least one cultivation layer (40) with at least one cultivation chamber (46) with a cultivation input (42) for receiving separated individual cell aggregates (ZA) from the at least one separating section (36) and a cultivation output (44) for discharging fluid from the cultivation chamber (46).

公开(公告)号：US12005452B2

公开(公告)日：2024-06-11

申请号：US17404478

申请日：2021-08-17

Applicant: Battelle Memorial Institute

Inventor： Ryan T. Kelly ,  Ying Zhu ,  Richard D. Smith

IPC: B01L3/00 ,  A61B10/02 ,  B01J19/00 ,  C12M1/00 ,  C12M1/34 ,  C12Q1/6881 ,  C40B60/04 ,  G01N1/28 ,  G01N1/30 ,  G01N1/34 ,  G01N30/88 ,  G01N33/483 ,  G01N33/68 ,  G02B21/32

CPC classification number: B01L3/502753 ,  A61B10/02 ,  B01J19/0046 ,  B01L3/00 ,  B01L3/50853 ,  B01L3/5088 ,  C12M23/58 ,  C12M41/00 ,  C12Q1/6881 ,  C40B60/04 ,  G01N1/2813 ,  G01N1/30 ,  G01N1/34 ,  G01N30/88 ,  G01N33/4833 ,  G01N33/68 ,  G02B21/32 ,  B01J2219/00317 ,  B01J2219/00367 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00659 ,  B01J2219/00702 ,  B01J2219/00725 ,  B01J2219/00743 ,  B01L2200/142 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2300/0887 ,  G01N2001/284 ,  G01N2030/8831

Abstract: Provided herein are methods and systems for biochemical analysis, including compositions and methods for processing and analysis of small cell populations and biological samples (e.g., a robotically controlled chip-based nanodroplet platform). In particular aspects, the methods described herein can reduce total processing volumes from conventional volumes to nanoliter volumes within a single reactor vessel (e.g., within a single droplet reactor) while minimizing losses, such as due to sample evaporation.

公开(公告)号：US20240150698A1

公开(公告)日：2024-05-09

申请号：US18383626

申请日：2023-10-25

Applicant: Sharp Display Technology Corporation

Inventor： Chihiro TACHINO ,  Tomoko TERANISHI ,  Satoshi IHIDA ,  Takeshi HARA

IPC: C12M1/12 ,  C12M1/00 ,  C12M1/34

CPC classification number: C12M25/14 ,  C12M23/22 ,  C12M41/00

Abstract: A cell culture device includes a substrate, a wire disposed on a main surface of the substrate, an insulating film disposed on the main surface of the substrate and having a part that overlaps the wire, and the electrodes disposed on the insulating film and having a part that overlaps the wire. The insulating film has a contact hole at a position overlapping the wire and the electrode. A plurality of grooves are formed on at least the surface of a part of the electrode that overlaps the contact hole.

公开(公告)号：US20240132825A1

公开(公告)日：2024-04-25

申请号：US18490429

申请日：2023-10-18

Applicant: CMDC LABS

Inventor： DANIEL M. STOREY ,  CHRISTINA K. THOMAS ,  SIENNA PEARL STOLL

IPC: C12M1/00 ,  C12M1/34 ,  C12M3/00

CPC classification number: C12M29/04 ,  C12M23/48 ,  C12M41/00

Abstract: The invention discloses a quasi in-vivo testing method using a uniquely designed multichambered bioblock apparatus. Cells are placed inside a top chamber fitted with a pore size membrane that allows certain cells to migrate through the pores into a bottom chamber holding a substrate. The portion of cells migrating from the top chamber and interacting with the substrate surface in a real time physiological quasi in vivo environment can be used to determine both the migratory response of specific cell types to selected attractive surfaces and the effect of the migratory cells on the surface over selected times of exposure.

公开(公告)号：US11939611B2

公开(公告)日：2024-03-26

申请号：US15436315

申请日：2017-02-17

Applicant: INV Nylon Chemicals Americas, LLC

Inventor： Gary Smith ,  Alex Van Eck Conradie ,  Gregory S. Kirby ,  Paul S. Pearlman

IPC: C12P13/00 ,  C12M1/00 ,  C12M1/34 ,  C12N1/20 ,  C12P5/00 ,  C12P5/02 ,  C12P7/06 ,  C12P7/24 ,  C12P7/625 ,  C25B1/04 ,  F01D15/10 ,  F01K7/16 ,  F01K11/02

CPC classification number: C12P13/001 ,  C12M29/00 ,  C12M41/00 ,  C12N1/20 ,  C12P5/00 ,  C12P5/007 ,  C12P5/02 ,  C12P7/065 ,  C12P7/24 ,  C12P7/625 ,  C12P13/00 ,  C25B1/04 ,  F01D15/10 ,  F01K7/16 ,  F01K11/02 ,  F05D2220/31 ,  Y02E50/10 ,  Y02E50/30 ,  Y02E60/36

Abstract: The present disclosure describes biocatalytic processes for producing a product, comprising providing an aqueous stream (AS) comprising at least one fermentable substrate and a gaseous stream (GS) comprising at least one of CO2/H2, H2, methane, and/or CO to a fermentation zone, wherein the GS and AS stream are optionally contacted and/or mixed; the fermentation zone comprising at least one organism capable of metabolizing an AS substrate and a GS substrate, wherein the fermentation operates at conditions to mixotrophically metabolize at least one gaseous substrate in the GS and at least one substrate in the AS, producing the product. The present disclosure also describes compositions comprising an AS, a GS, and an organism, wherein the organism or an equivalent or engineered equivalent thereof is a methanotroph or a hydrogen-metabolizing or CO-metabolizing chemolithotrophic organism, and wherein the organism is capable of mixotrophic metabolism of at least one gaseous substrate in the GS and at least one substrate in the AS. The present disclosure also describes a process wherein said fermentation operates at conditions to mixotrophically metabolize at least H2 in the gaseous stream and glycerol and lactic acid in the aqueous stream. The present disclosure also describes a system for producing a fermentation or bio-derived product.

公开(公告)号：US11834646B2

公开(公告)日：2023-12-05

申请号：US17752345

申请日：2022-05-24

Applicant: Repligen Corporation

Inventor： Rudolf Pavlik

IPC: F04B49/20 ,  F04B23/04 ,  F04B43/00 ,  F04B49/06 ,  B01D61/22 ,  B01D61/58 ,  B01D63/02 ,  B01D61/20 ,  F04B23/02 ,  C12M1/36 ,  C12M1/34 ,  C12M1/00 ,  F04B43/073

CPC classification number: C12M41/48 ,  B01D61/20 ,  B01D61/22 ,  B01D61/58 ,  B01D63/02 ,  C12M29/00 ,  C12M29/04 ,  C12M29/18 ,  C12M41/00 ,  C12M41/44 ,  C12M47/02 ,  C12M47/10 ,  F04B23/04 ,  F04B43/0081 ,  F04B43/073 ,  F04B49/065 ,  F04B49/20 ,  B01D2311/2688 ,  F04B23/02 ,  F04B2205/09

Abstract: A control unit includes a first connector configured to connect a proportional pressure regulator to a positive pressure supply and a second connector configured to connect the proportional pressure regulator to a negative pressure supply. The control unit further includes at least one sensor configured to detect an amount of air flow (volume per unit of time), positive or negative, within an air flow line connected to an output of the proportional pressure regulator, and a third connector configured to connect the air flow line to an air side of a diaphragm. Additionally, the control unit includes a controller programmed to control at least an opening and closing function of the proportional pressure regulator to attain a desired amount of air flow (volume per unit of time), positive or negative, within the air flow line.

公开(公告)号：US11827874B2

公开(公告)日：2023-11-28

申请号：US17156701

申请日：2021-01-25

Applicant: Sartorius Stedim Biotech GmbH

Inventor： Bernward Husemann ,  Simon Topp-Manske ,  Bjoern Nickel ,  Marco Leupold

IPC: C12M1/00 ,  C12M3/00 ,  C12M1/34 ,  C12M1/06

CPC classification number: C12M29/26 ,  C12M23/14 ,  C12M23/22 ,  C12M23/26 ,  C12M23/28 ,  C12M23/38 ,  C12M23/48 ,  C12M27/02 ,  C12M41/00 ,  C12M41/12

Abstract: A bioreactor (1; 2; 3) includes a receiving container (10) for receiving a disposable bag (44). A bottom surface (11) of the receiving container (10) is designed to support the disposable bag (44) in the receiving container (10). An outlet opening (5) is formed in the bottom surface (11) and tapers down with respect to the ground for receiving a drain port (45) of the disposable bag (44). The drain port is complementary to the outlet opening (5).

公开(公告)号：US20230357713A1

公开(公告)日：2023-11-09

申请号：US18207304

申请日：2023-06-08

Applicant: PLURI BIOTECH LTD.

Inventor： Lior RAVIV ,  Eran BEN ELIEZER

IPC: C12N5/04 ,  C12M1/12 ,  C12M1/26 ,  C12M1/06 ,  C12M1/00 ,  C12M1/34

CPC classification number: C12N5/04 ,  C12M25/18 ,  C12M33/08 ,  C12M25/02 ,  C12M27/02 ,  C12M29/00 ,  C12M41/00

Abstract: This invention is directed to a method for three-dimensional cultivation of proliferating plant cells in a packed bed bioreactor, the bioreactor comprising at least one packed bed chamber configured to confine said proliferating plant cells within it such that the proliferating plant cells become a three-dimensional stationary biomass phase; and At least one container comprising a fluid media, the fluid media is configured to flow through said proliferating plant cells stationary biomass phase, wherein, the flow of the fluid media through the proliferating plant cells stationary biomass phase allows transfer of compounds from the fluid media into the cells and vice versa in low sheer forces within the at least one packed bed chamber thereby imitating natural growth environment of the proliferating plant cells within the 3D bioreactor.