公开(公告)号：US20250032997A1

公开(公告)日：2025-01-30

申请号：US18781201

申请日：2024-07-23

Applicant: Repligen Corporation

Inventor： Michael Bransby ,  Philip Yuen ,  Derek Carroll

IPC: B01D69/08 ,  B01D71/26 ,  B01D71/34 ,  B01D71/36 ,  C12M1/00

Abstract: The present invention relates to hollow fiber tangential flow depth filtration media adapted for perfusion bioprocessing, and related TFDF filtration units, bioprocessing systems, and methods, including methods for perfusion cell culture. The hollow fiber elements of the present invention combine a relatively thin porous wall with a relatively low porosity to provide filtration media resistant to fouling during perfusion culture.

公开(公告)号：US20240133472A1

公开(公告)日：2024-04-25

申请号：US18405525

申请日：2024-01-05

Applicant: Repligen Corporation

Inventor： Michael C. Gagne ,  Steven V. Cates ,  Dean C. Richards ,  Scott Bendon

IPC: F16K11/22 ,  B29C45/14 ,  B29C65/00 ,  F16K7/06 ,  F16K7/07 ,  F16K27/00 ,  F16K27/02

CPC classification number: F16K11/22 ,  B29C45/14614 ,  B29C66/01 ,  F16K7/06 ,  F16K7/07 ,  F16K27/003 ,  F16K27/0236 ,  F16B13/00

Abstract: A valve assembly with a multidirectional flow path includes a valve body that includes respective hinged portions defining respective passageways extending through the valve body when in a closed state. A flexible conduit or tubing having a loop portion and a plurality of branches extending from the loop portion is at least partially positioned within the valve body. Portions of the loop that extend beyond the valve body are encapsulated by respective rigid jackets. Multiple valves are situated on the valve body and are used to selectively pinch the loop portion in different pinching configurations to direct fluid to or from the particular branches. The valve assembly may be integrated into a manufacturing or other production process with optional additional jackets used to encapsulate the flexible conduit branches.

公开(公告)号：US11958018B2

公开(公告)日：2024-04-16

申请号：US17091616

申请日：2019-05-28

Applicant: REPLIGEN CORPORATION

Inventor： Michael Bransby ,  Derek Carroll ,  Philip Yuen ,  Ralf Kuriyel

IPC: B01D61/14 ,  B01D63/02 ,  B01D63/06 ,  B01D65/08 ,  C12M1/26

CPC classification number: B01D63/06 ,  B01D61/147 ,  B01D63/02 ,  B01D65/08 ,  C12M33/14 ,  B01D2315/10 ,  B01D2321/20

Abstract: The disclosure provides tangential flow depth filtration (TFDF) systems which exhibit improved filter fluxes and process capacities and reduced fouling characteristics. The TFDF systems of the disclosure optionally utilize tubular depth filters (TDF). Methods are provided which include the passage of a non-laminar flow through at least a portion of a length of a TDF in a TFDF system.

公开(公告)号：US20240091671A1

公开(公告)日：2024-03-21

申请号：US18460843

申请日：2023-09-05

Applicant: Repligen Corporation

Inventor： Erik Fiedler ,  Ulrich Haupts

IPC: B01D15/38 ,  B01J20/32 ,  C07K1/22 ,  C07K14/31

CPC classification number: B01D15/3809 ,  B01J20/3274 ,  C07K1/22 ,  C07K14/31

Abstract: The present invention relates to Fc binding proteins comprising one or more domains with Cysteine in the C-terminal helical region. The invention further relates to affinity matrices comprising the Fc binding proteins of the invention. The invention also relates to a use of these Fc binding proteins or affinity matrices for affinity purification of immunoglobulins and to methods of affinity purification using the Fc binding proteins of the invention.

公开(公告)号：US11834646B2

公开(公告)日：2023-12-05

申请号：US17752345

申请日：2022-05-24

Applicant: Repligen Corporation

Inventor： Rudolf Pavlik

IPC: F04B49/20 ,  F04B23/04 ,  F04B43/00 ,  F04B49/06 ,  B01D61/22 ,  B01D61/58 ,  B01D63/02 ,  B01D61/20 ,  F04B23/02 ,  C12M1/36 ,  C12M1/34 ,  C12M1/00 ,  F04B43/073

CPC classification number: C12M41/48 ,  B01D61/20 ,  B01D61/22 ,  B01D61/58 ,  B01D63/02 ,  C12M29/00 ,  C12M29/04 ,  C12M29/18 ,  C12M41/00 ,  C12M41/44 ,  C12M47/02 ,  C12M47/10 ,  F04B23/04 ,  F04B43/0081 ,  F04B43/073 ,  F04B49/065 ,  F04B49/20 ,  B01D2311/2688 ,  F04B23/02 ,  F04B2205/09

Abstract: A control unit includes a first connector configured to connect a proportional pressure regulator to a positive pressure supply and a second connector configured to connect the proportional pressure regulator to a negative pressure supply. The control unit further includes at least one sensor configured to detect an amount of air flow (volume per unit of time), positive or negative, within an air flow line connected to an output of the proportional pressure regulator, and a third connector configured to connect the air flow line to an air side of a diaphragm. Additionally, the control unit includes a controller programmed to control at least an opening and closing function of the proportional pressure regulator to attain a desired amount of air flow (volume per unit of time), positive or negative, within the air flow line.

公开(公告)号：US20230375407A1

公开(公告)日：2023-11-23

申请号：US18198513

申请日：2023-05-17

Applicant: Repligen Corporation

Inventor： Matthew Muller ,  Richard Hall, III ,  Yusheng Zhang ,  Peter Halatin

IPC: G01J3/42 ,  G01N21/31 ,  G01J3/10 ,  G01N21/25

CPC classification number: G01J3/42 ,  G01N21/3103 ,  G01J3/10 ,  G01N21/255

Abstract: A method includes determining whether a variation in probe radiation intensity meets a stability criterion; directing the probe radiation through a probe, when the probe is disposed at a first position, defining a first path length L1 of the probe radiation through the fluid sample; measuring a transmitted intensity I1 of the probe radiation after passing through the fluid sample when the probe is disposed at the first position; directing the probe radiation through the probe when the probe is disposed at a second position, defining a second path length L2 of the probe radiation through the fluid sample; measuring a transmitted intensity I2 of the probe radiation after passing through the fluid sample when the probe is disposed at the second position; and determining a concentration C of a material in the fluid sample based upon L1, I1, L2, and I2, when the stability criterion is met.

公开(公告)号：US11813573B2

公开(公告)日：2023-11-14

申请号：US17869077

申请日：2022-07-20

Applicant: REPLIGEN CORPORATION

Inventor： Rudolf Pavlik

IPC: B01D63/02 ,  B01D69/08 ,  B01D37/04 ,  B01D35/26 ,  B01D29/11 ,  B01D29/90 ,  F04B43/00 ,  F04B43/06 ,  F04B53/20 ,  C12M1/00 ,  C12M1/12 ,  F04B43/067

CPC classification number: B01D63/02 ,  B01D29/11 ,  B01D29/908 ,  B01D35/26 ,  B01D37/04 ,  B01D69/08 ,  C12M25/02 ,  C12M29/10 ,  F04B43/0063 ,  F04B43/0081 ,  F04B43/067 ,  F04B53/20 ,  B01D2313/243 ,  B01D2313/58 ,  B01D2313/90

Abstract: A fluid filtration assembly includes a filter housing having a first end for fluid connection with a fluid storage vessel. A filter cartridge is disposable within the filter housing, and a plunger pump is coupled at a second end of the filter housing. The plunger pump includes a housing having a rigid portion and a flexible portion. The flexible portion has a plunger-engaging portion for coupling to the plunger of an actuator. The flexible portion selectively movable with respect to the rigid portion via the actuator. The filter cartridge can be a hollow fiber filter. The plunger pump can be configured to induce alternating tangential flow in at least a portion of the assembly. The fluid filtration assembly can be provided as a disposable single-use arrangement.

公开(公告)号：US20230338872A1

公开(公告)日：2023-10-26

申请号：US18218143

申请日：2023-07-05

Applicant: Repligen Corporation

Inventor： James Ronald Peyser ,  Dana Cristina Pentia

IPC: B01D15/20 ,  A61L2/08

CPC classification number: B01D15/206 ,  A61L2/081 ,  A61L2/087 ,  B01D15/20 ,  B01D15/3809

Abstract: If one sterilizes pre-packed, plastic chromatography columns with an appropriate level of gamma irradiation, the resulting sterile chromatography columns maintain sufficient packing media function and maintain column mechanical properties and pressure ratings.

公开(公告)号：US11680239B2

公开(公告)日：2023-06-20

申请号：US16730516

申请日：2019-12-30

Applicant: Repligen Corporation

Inventor： Michael Bransby ,  Derek Carroll ,  Philip Yuen

IPC: C12M1/12 ,  C12M3/00 ,  B01D69/08 ,  B01D63/04 ,  C12M1/00 ,  B01D71/26

CPC classification number: C12M25/10 ,  B01D63/04 ,  B01D69/081 ,  B01D71/26 ,  C12M21/08 ,  C12M29/10 ,  B01D2239/0428 ,  B01D2239/1216 ,  B01D2239/1225

Abstract: This disclosure relates to the use of a hydrophobic hollow fiber filter for the filtration of cell cultures and other biological perfusions, due to its resistance to fouling, as well as the ability to filter solutions with a high solid content. A hydrophobic hollow fiber filter may be used within a filter housing in conjunction with a process vessel and a traditional separation system. When the system is used with alternating tangential flow or tangential flow filtration, the hydrophobic hollow fiber filter results in more effective filtration of the filtrate, leading to greater concentration of the retentate, even in solution containing high levels of solids.

公开(公告)号：US20220193614A1

公开(公告)日：2022-06-23

申请号：US17559792

申请日：2021-12-22

Applicant: Repligen Corporation

Inventor： Bao Le ,  Philip Yuen

IPC: B01D63/02 ,  B01D19/00

Abstract: Devices, systems, and methods for continually degassing/venting a feedstream within a filtration system. The filtration system may include a filter device utilizing hollow fiber filters to filter the feedstream. Additional venting hollow fiber filters vent gas from the filtration system to outside the filtration system. The venting hollow fiber filters may have ends which expose the interior lumens thereof to outside the filter unit to vent gases which pass through the walls of the hollow fiber filters. Degassed permeate thus flows through the filtering hollow fiber filters.