公开(公告)号：US20250034512A1

公开(公告)日：2025-01-30

申请号：US18275380

申请日：2023-03-30

Applicant: QILU UNIVERSITY OF TECHNOLOGY (SHANDONG ACADEMY OF SCIENCES)

Inventor： Lujiang HAO ,  Yuhao JU ,  Wei WANG ,  Kai SHAN ,  Xiaofei ZHANG ,  Ruiwen CAO ,  Nan LIU ,  Gangrui ZHANG ,  Xingbao WANG

IPC: C12N1/20 ,  B01D15/36 ,  C12P19/04 ,  C12R1/01

Abstract: The present disclosure provides a Pseudoalteromonas agarivorans strain and a preparation method of an exopolysaccharide (EPS), and relates to the technical field of functional bacteria. In the present disclosure, the Pseudoalteromonas agarivorans strain HJWL2022 has a deposit number of CGMCC No. 26272. Fermentation, centrifugation, alcohol precipitation, protein removal, purification, and drying are conducted with the strain HJWL2022 to obtain a pure EPS. A proportion of a monosaccharide component in the product EPS is controlled by precisely controlling a pH value in a fermentation environment of the strain HJWL2022. Targeted control of a monosaccharide ratio is achieved to enhance a certain biological activity by adjusting fermentation conditions to change a composition of a bacterial EPS.

公开(公告)号：US12203119B2

公开(公告)日：2025-01-21

申请号：US17340466

申请日：2021-06-07

Applicant: Jiangnan University

Inventor： Bo Jiang ,  Tao Zhang ,  Luhua Zheng ,  Xiaolin Yao

IPC: C12P19/04

Abstract: The disclosure discloses a method for producing maltodextrin with a single polymerization degree by multienzyme coupling, and belongs to the technical field of biology. The disclosure provides a method for producing non-reducing maltodextrin with a uniform and low polymerization degree. After a reaction is preformed for 2-6 hours by using the method of the disclosure, the content of 4-O-α-maltohexaosyl α-D-glucoside in a reaction solution can be as high as 57.2% to 77.3%, accounting for 50% to 90% of the total amount of maltodextrin in the reaction solution. In maltodextrin prepared by using the method of the disclosure, non-reducing maltodextrin with low polymerization degree only includes 4-O-α-maltohexaosyl α-D-glucoside, and the content of non-reducing maltodextrin with low polymerization degree can be 50% to 90% of the total amount of maltodextrin. Therefore, only filtration is needed in preparation of non-reducing maltodextrin with low polymerization degree by using the method of the disclosure; additional separation and purification steps are not needed; high-purity non-reducing maltodextrin with low polymerization degree can be obtained; and the production cost is low.

公开(公告)号：US20250011381A1

公开(公告)日：2025-01-09

申请号：US18661777

申请日：2024-05-13

Applicant: NUTRITION & BIOSCIENCES USA 4, INC.

Inventor： QIONG CHENG ,  ROBERT DICOSIMO ,  JAHNAVI CHANDRA PRASAD ,  ZHENGHONG ZHANG

IPC: C07K14/47 ,  A23L33/10 ,  A23L33/135 ,  A23L33/21 ,  A61K8/60 ,  A61K8/64 ,  A61K8/73 ,  A61K31/716 ,  A61Q19/00 ,  C08B37/00 ,  C08L5/00 ,  C08L5/02 ,  C11D3/22 ,  C12N9/10 ,  C12P19/04 ,  C12P19/18

Abstract: Disclosed herein are proteins capable of forming glucans having alpha-1,2 linkages/branches, reactions and methods for producing such glucan, compositions comprising such glucan, and various applications thereof.

公开(公告)号：US12188055B2

公开(公告)日：2025-01-07

申请号：US18157512

申请日：2023-01-20

Applicant: NUTRITION & BIOSCIENCES USA 4, INC.

Inventor： Yougen Li ,  Ellen D. Semke ,  Qiong Cheng ,  Jian Ping Lai

IPC: C12N9/10 ,  C08B37/00 ,  C12N15/56 ,  C12P19/04 ,  C12P19/18 ,  G16B25/00

Abstract: Disclosed herein are glucosyltransferases with modified amino acid sequences. Such engineered enzymes exhibit improved alpha-glucan product yields and/or lower leucrose yields, for example. Further disclosed are reactions and methods in which engineered glucosyltransferases are used to produce alpha-glucan.

公开(公告)号：US20240425892A1

公开(公告)日：2024-12-26

申请号：US18826686

申请日：2024-09-06

Applicant: Modern Synthesis Ltd.

Inventor： Jennifer Killeen Keane

IPC: C12P19/04 ,  C12M1/12 ,  D06M16/00 ,  D06M101/06

Abstract: A mesh reinforced bacterial cellulose hybrid composition, such as a film, includes a two-dimensional mesh fully incorporated into a BC sheet. Such materials can be fabricated by providing a bacterial cellulose-producing bacterium; providing a suitable bacteria nutritional medium; culturing the bacterium in the nutritional medium under conditions to produce bacterial cellulose; and providing a two-dimensional mesh, which can have openings distributed throughout a lateral area of the mesh through which the BC extends uninhibited by the mesh, such that the mesh is fully incorporated into the bacterial cellulose.

公开(公告)号：US20240417771A1

公开(公告)日：2024-12-19

申请号：US18705359

申请日：2022-10-27

Applicant: SOCIETE DES PRODUITS NESTLE S.A.

Inventor： MARIA MARQUES DE LIMA ,  VIRGINIE BACHMANN ,  ARIANNA BIANCHI ,  OMID MASHINCHIAN ,  MARINE KRAUS

IPC: C12P21/02 ,  C12N5/071 ,  C12P19/04

Abstract: A method for producing mammary gland cells and a method of producing a mammalian milk like product, for example a human milk like product comprising generating lactocytes derived from mammalian induced pluripotent stem cells (miPSC), for example human induced pluripotent stem cells (hiPSC), and expressing the mammalian milk like product, for example the human milk like product from lactocytes.

公开(公告)号：US20240417768A1

公开(公告)日：2024-12-19

申请号：US18720533

申请日：2022-12-14

Applicant: INBIOSE N.V.

Inventor： Joeri Beauprez ,  Kristof Vandewalle ,  Annelies Vercauteren

IPC: C12P19/18 ,  C07H15/12 ,  C12N9/10 ,  C12P19/04

Abstract: The present invention is in the technical field of synthetic biology, metabolic engineering and cell cultivation. The present invention describes methods for the production of a fucosylated compound comprising Gal-β1,3-[Fuc-α1,4]-GlcNAc using a fucosyltransferase as well as the purification of said fucosylated compound, said fucosyltransferase having alpha-1,4-fucosyltransferase activity on the N-acetylglucosamine (GlcNAc) residue of Gal-β1,3-GlcNAc (lacto-N-biose, LNB) and/or Gal-β1,3-GlcNAc of a saccharide substrate comprising Gal-β1,3-GlcNAc. The present invention also provides a cell for production of a fucosylated compound comprising Gal-β1,3-[Fuc-α1,4]-GlcNAc.

公开(公告)号：US20240417767A1

公开(公告)日：2024-12-19

申请号：US18718677

申请日：2022-12-14

Applicant: INBIOSE N.V.

Inventor： Joeri Beauprez ,  Kristof Vandewalle ,  Annelies Vercauteren

IPC: C12P19/04 ,  C07H1/08 ,  C12N9/10 ,  C12P19/18

Abstract: This disclosure is in the technical field of synthetic biology, metabolic engineering and cell cultivation. The disclosure describes methods for the production of a fucosylated compound using a fucosyltransferase as well as the purification of the fucosylated compound, the fucosyltransferase having alpha-1,3-fucosyltransferase activity on the N-acetylglucosamine (GlcNAc) and/or the glucose (Glc) residue of Gal-β1,m-GlcNAc-β1,n-Gal-β1,4-Glc of a saccharide substrate comprising Gal-β1,m-GlcNAc-β1,n-Gal-β1,4-Glc wherein the m is 3 or 4 and the n is 3 or 6. The disclosure also provides a cell for production of a fucosylated compound. Next, the disclosure describes methods for the production of 3-fucosyllactose (3-FL) using a fucosyltransferase having alpha-1,3-fucosyltransferase activity on the Glc residue of lactose, as well as the purification of the 3-FL. The disclosure also provides a cell for production of 3-FL.

公开(公告)号：US20240417766A1

公开(公告)日：2024-12-19

申请号：US18561170

申请日：2022-05-17

Applicant: DSM IP Assets B.V.

Inventor： Manos PAPADAKIS

IPC: C12P19/04 ,  C12N1/20 ,  C12N9/10 ,  C12N9/26 ,  C12N15/70 ,  C12P19/18 ,  C12R1/19

Abstract: The present disclosure discloses the identification and introduction of a specific heterologous gene (denoted as smob), which encodes an α-1,2-fucosyltransferase, into an LNT production strain to produce LNFP-I in particular.
The smob gene originates from the organism Sulfuriflexus mobilis (https://www.dsmz.de/collection/catalogue/details/culture/DSM-102939), which is a sulfur-oxidizing bacterium isolated from a brackish lake sediment.
FIG. 1 should accompany the abstract.

公开(公告)号：US20240417765A1

公开(公告)日：2024-12-19

申请号：US18549668

申请日：2022-03-10

Applicant: Chr. Hansen A/S

Inventor： Maria Elina Sundberg ,  Vojislav Vojinovic ,  Hans Raj

IPC: C12P19/04 ,  A23C9/12

Abstract: The present invention relates to a fermented milk-based product comprising a combination of low levels of lactose and stable levels of galacto-oligosaccharides (GOS) produced in-situ and methods thereof.