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公开(公告)号：US11821824B2
公开(公告)日：2023-11-21
申请号：US15707270
申请日：2017-09-18
Applicant: Koligo Therapeutics, Inc.
Inventor： Rolf Wolters , Anthony Yang , Josh Nelson , Stuart K. Williams
IPC: C12M3/00 , B01L3/00 , A61M1/00 , G01N1/34 , C12N5/077 , G01N1/40 , C12N5/0775 , A61K35/35 , C12M1/34
CPC classification number: G01N1/34 , A61K35/35 , A61M1/79 , C12M41/00 , C12N5/0653 , C12N5/0667 , G01N1/4044 , A61M1/73 , A61M2202/0014 , A61M2202/0071 , A61M2202/0085 , A61M2202/08 , A61M2202/09 , C12N2509/10
Abstract: Systems and methods herein are directed towards the separation of biologic material to obtain a target cell volume and/or cell concentration for harvesting. The target volume and/or concentration of cells may be obtained through a single cycle via three chambers, or by repeated cycles through one or more chambers to dilute the digestive enzymes used in the process and concentrate the harvestable cell volume to a predetermined target.
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公开(公告)号：US11905509B2
公开(公告)日：2024-02-20
申请号：US17512558
申请日：2021-10-27
Applicant: Koligo Therapeutics, Inc
Inventor： Rolf Wolters , Jules Valenti , Tom Gurski
CPC classification number: C12M47/04 , A61K35/12 , B04B1/00 , B04B1/02 , C12M33/10 , C12M45/05 , C12M45/06 , C12M45/09
Abstract: Cell separation systems and methods of separating cells are disclosed. In an embodiment, a cell separation system is described that comprises a non-transitory storage device that executes a centrifugation program to separate cell volume from biologic material volume; a heating mechanism; a containment mechanism; and an assembly comprised of a single-walled centrifugation bowl. In an embodiment, methods of separating cells are disclosed whereby cells are separated by agitating a volume of biologic material and a volume digestion media to form a digested volume of biologic material; centrifuging the digested volume of biologic material; removing a portion of a resulting waste via at least one fluid outlet; isolating a different portion of the waste, and removing the concentrated cell volumes from the reservoir.
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公开(公告)号：US11584912B2
公开(公告)日：2023-02-21
申请号：US14868266
申请日：2015-09-28
Applicant: Koligo Therapeutics, Inc.
Inventor： Gregory D. Ariff , Thomas Cannon , Jennifer L. Case , Christian L. Haller , Paul Kosnik , Charles P. Luddy , Craig A. Mauch , Erik Vossman , Stuart K. Williams
Abstract: The present invention provides automated devices for use in supporting various cell therapies and tissue engineering methods. The present invention provides an automated cell separation apparatus capable of separating cells from a tissue sample for use in cell therapies and/or tissue engineering. The cell separation apparatus can be used in combination with complementary devices such as cell collection device and/or a sodding apparatus to support various therapies. The automated apparatus includes media and tissue dissociating chemical reservoirs, filters, a cell separator and a perfusion flow loop through a graft chamber which supports a graft substrate or other endovascular device. The present invention further provides methods for using the tissue grafts and cell samples prepared by the devices described herein in a multitude of therapies including revascularization, regeneration and reconstruction of tissues and organs, as well as treatment and prevention of diseases.
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公开(公告)号：US11174458B2
公开(公告)日：2021-11-16
申请号：US16219001
申请日：2018-12-13
Applicant: Koligo Therapeutics, Inc.
Inventor： Rolf Wolters , Jules Valenti , Tom Gurski
Abstract: Cell separation systems and methods of separating cells are disclosed. In an embodiment, a cell separation system is described that comprises a non-transitory storage device that executes a centrifugation program to separate cell volume from biologic material volume; a heating mechanism; a containment mechanism; and an assembly comprised of a single-walled centrifugation bowl. In an embodiment, methods of separating cells are disclosed whereby cells are separated by agitating a volume of biologic material and a volume digestion media to form a digested volume of biologic material; centrifuging the digested volume of biologic material; removing a portion of a resulting waste via at least one fluid outlet; isolating a different portion of the waste, and removing the concentrated cell volumes from the reservoir.
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