公开(公告)号：US12097495B2

公开(公告)日：2024-09-24

申请号：US17750195

申请日：2022-05-20

申请人： Bio-Rad Laboratories, Inc.

发明人： Benjamin J. Hindson ,  Serge Saxonov ,  Phillip Belgrader ,  Kevin D. Ness ,  Michael Y. Lucero ,  Billy W. Colston, Jr. ,  Shawn Paul Hodges ,  Nicholas J. Heredia ,  Jeffrey Clark Mellen ,  Camille Bodley Troup ,  Paul Wyatt

IPC分类号： B01L3/00 ,  B01F23/41 ,  B01F33/3011 ,  B01F33/81 ,  B01L9/00 ,  C12Q1/686 ,  B01F25/00 ,  B01F25/23 ,  B01F33/302 ,  G01N35/08

CPC分类号： B01L3/50273 ,  B01F23/41 ,  B01F33/3011 ,  B01F33/813 ,  B01L3/502 ,  B01L3/502784 ,  B01L3/52 ,  B01L9/527 ,  C12Q1/686 ,  B01F25/14 ,  B01F25/23 ,  B01F33/3021 ,  B01L3/502761 ,  B01L2200/025 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/14 ,  B01L2200/16 ,  B01L2300/0609 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0861 ,  B01L2400/049 ,  G01N35/08

摘要： The present disclosure provides methods and compositions for detecting polynucleotides in a sample and for quantifying polynucleotide load in a sample. The polynucleotides can be associated with a disease, disorder, or condition. In some applications, methylated DNA is quantified, e.g., in order to determine the load of polynucleotides in a sample. The present disclosure also provides methods and compositions for determining the load of fetal polynucleotides in a biological sample, e.g., the load of fetal polynucleotides (e.g., DNA, RNA) in maternal plasma. The present disclosure provides methods and compositions for detecting cellular processes such as cellular viability, growth rates, and infection rates. This disclosure also provides compositions and methods for detecting differences in copy number of a target polynucleotide. In some embodiments, the methods and compositions provided herein are useful for diagnosis of fetal genetic abnormalities, when the starting sample is maternal tissue (e.g., blood, plasma). The methods and materials described apply techniques for allowing detection of small, but statistically significant, differences in polynucleotide copy number.

公开(公告)号：US12091710B2

公开(公告)日：2024-09-17

申请号：US17174519

申请日：2021-02-12

申请人： Bio-Rad Laboratories, Inc.

发明人： Darren Roy Link

IPC分类号： C12Q1/6848 ,  B01F23/41 ,  B01F33/3011 ,  B01J19/00 ,  B01L3/00 ,  B03C5/00 ,  B03C5/02 ,  C12N15/10 ,  C12Q1/00 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6874 ,  G01N1/38 ,  G01N15/14 ,  G01N35/00 ,  G01N35/08 ,  B01L7/00 ,  B01L9/00

CPC分类号： C12Q1/6848 ,  B01F23/41 ,  B01F33/3011 ,  B01J19/0093 ,  B01L3/502715 ,  B01L3/502753 ,  B01L3/502776 ,  B01L3/502784 ,  B01L3/565 ,  B03C5/005 ,  B03C5/026 ,  C12N15/1075 ,  C12Q1/00 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6874 ,  G01N1/38 ,  G01N15/147 ,  G01N35/08 ,  B01J2219/00783 ,  B01J2219/00788 ,  B01J2219/00828 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00837 ,  B01J2219/00853 ,  B01J2219/0086 ,  B01J2219/00869 ,  B01J2219/00889 ,  B01J2219/00903 ,  B01J2219/0097 ,  B01J2219/00988 ,  B01L3/502746 ,  B01L7/525 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2200/141 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/165 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0487 ,  B01L2400/086 ,  B03C2201/26 ,  C12Q2525/191 ,  C12Q2535/101 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/628 ,  C12Q2565/629 ,  G01N2035/00158 ,  G01N2035/00237 ,  G01N2035/00326

摘要： The invention generally relates to assemblies for displacing droplets from a vessel that facilitate the collection and transfer of the droplets while minimizing sample loss. In certain aspects, the assembly includes at least one droplet formation module, in which the module is configured to form droplets surrounded by an immiscible fluid. The assembly also includes at least one chamber including an outlet, in which the chamber is configured to receive droplets and an immiscible fluid, and in which the outlet is configured to receive substantially only droplets. The assembly further includes a channel, configured such that the droplet formation module and the chamber are in fluid communication with each other via the channel. In other aspects, the assembly includes a plurality of hollow members, in which the hollow members are channels and in which the members are configured to interact with a vessel. The plurality of hollow members includes a first member configured to expel a fluid immiscible with droplets in the vessel and a second member configured to substantially only droplets from the vessel. The assembly also includes a main channel, in which the second member is in fluid communication with the main channel. The assembly also includes at least one analysis module connected to the main channel.

公开(公告)号：US11987789B2

公开(公告)日：2024-05-21

申请号：US17230582

申请日：2021-04-14

申请人： Purigen Biosystems, Inc.

发明人： Lewis A. Marshall ,  Amy L. Hiddessen ,  Nathan P. Hoverter ,  Klint A. Rose ,  Juan G. Santiago ,  Matthew S. Munson ,  Janine Mok ,  Sean Arin ,  Yatian Qu ,  Andrew Lee ,  Michael Christopher De Renzi

IPC分类号： B01L3/00 ,  B01L9/00 ,  C12N15/10 ,  G01N27/447

CPC分类号： C12N15/101 ,  B01L3/502738 ,  B01L3/502746 ,  B01L3/502761 ,  B01L3/502776 ,  B01L3/5085 ,  G01N27/44717 ,  B01L9/527 ,  B01L2200/0621 ,  B01L2200/0642 ,  B01L2400/0421 ,  B01L2400/0688 ,  G01N27/44704 ,  G01N27/44743 ,  G01N27/44791

摘要： The present disclosure relates to fluidic systems and devices for processing, extracting, or purifying one or more analytes. These systems and devices can be used for processing samples and extracting nucleic acids, for example by isotachophoresis. In particular, the systems and related methods can allow for extraction of nucleic acids, including non-crosslinked nucleic acids, from samples such as tissue or cells. The systems and devices can also be used for multiplex parallel sample processing.

公开(公告)号：US20240110236A1

公开(公告)日：2024-04-04

申请号：US18486391

申请日：2023-10-13

申请人： Becton, Dickinson and Company

发明人： Thomas Catalino Gubatayao ,  Kalyan Handique ,  Karthik Ganesan ,  Daniel M. Drummond

IPC分类号： C12Q1/686 ,  B01L7/00 ,  B01L9/00 ,  G01N21/64 ,  G01N35/00 ,  G01N35/02

CPC分类号： C12Q1/686 ,  B01L7/52 ,  B01L9/527 ,  G01N21/6428 ,  G01N21/6452 ,  G01N35/00693 ,  G01N35/026 ,  G01N21/274

摘要： Systems and methods for performing simultaneous nucleic acid amplification and detection. The systems and methods comprise methods for managing a plurality of protocols in conjunction with directing a sensor array across each of a plurality of reaction chambers. In certain embodiments, the protocols comprise thermocycling profiles and the methods may introduce offsets and duration extensions into the thermocycling profiles to achieve more efficient detection behavior.

公开(公告)号：US11920114B2

公开(公告)日：2024-03-05

申请号：US17176873

申请日：2021-02-16

申请人： EMULATE, Inc.

发明人： Daniel Levner ,  Josiah Daniel Sliz ,  Christopher David Hinojosa ,  Joshua Gomes ,  Jose Fernandez-Alcon

IPC分类号： C12M3/06 ,  A01N1/02 ,  B01L3/00 ,  B01L9/00 ,  C12M1/00 ,  C12M1/34 ,  C12M1/36 ,  C12M1/42 ,  C12M3/00 ,  C12N5/071

CPC分类号： C12M23/16 ,  A01N1/021 ,  A01N1/0247 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L9/527 ,  C12M21/08 ,  C12M23/38 ,  C12M23/40 ,  C12M23/42 ,  C12M29/10 ,  C12M35/04 ,  C12M41/40 ,  C12M41/48 ,  C12N5/0602 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/12 ,  B01L2300/0681 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2300/14 ,  B01L2300/161 ,  B01L2300/165 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/06 ,  C12N2521/00

摘要： A culture module is contemplated that allows the perfusion and optionally mechanical actuation of one or more microfluidic devices, such as organ-on-a-chip microfluidic devices comprising cells that mimic at least one function of an organ in the body. A method for pressure control is contemplated to allow the control of flow rate (while perfusing cells) despite limitations of common pressure regulators. The method for pressure control allows for perfusion of a microfluidic device, such as an organ on a chip microfluidic device comprising cells that mimic cells in an organ in the body, that is detachably linked with said assembly, so that fluid enters ports of the microfluidic device from a fluid reservoir, optionally without tubing, at a controllable flow rate.

公开(公告)号：US11857977B2

公开(公告)日：2024-01-02

申请号：US17003187

申请日：2020-08-26

申请人： Tsinghua University

发明人： Moran Wang ,  Wenhai Lei

IPC分类号： B01L9/00

CPC分类号： B01L9/527 ,  B01L2200/10

摘要： A fixing clamp for a microfluidic chip includes a set of sub-clamps arranged to be spaced apart on left and right. Each of the sub-clamps includes an upper plate body with a first passage, the first passage having a first outer interface and a first chip docking port, and the first chip docking port being located on a lower surface of the upper plate body and at one end of the upper plate body facing the other sub-clamp; a lower plate body with a second passage, the second passage having a second outer interface and a second chip docking port, and the second chip docking port being located on an upper surface of the lower plate body and at one end of the lower plate body facing the other sub-clamp; and a spacing adjusting mechanism connecting the upper plate body and the lower plate body together.

公开(公告)号：US20230383280A1

公开(公告)日：2023-11-30

申请号：US18231601

申请日：2023-08-08

申请人： Purigen Biosystems, Inc.

发明人： Lewis A. Marshall ,  Amy L. Hiddessen ,  Nathan P. Hoverter ,  Klint A. Rose ,  Juan G. Santiago ,  Matthew S. Munson ,  Janine Mok ,  Sean Arin ,  Yatian Qu ,  Andrew Lee ,  Michael Christopher De Renzi

IPC分类号： C12N15/10 ,  B01L3/00 ,  G01N27/447

CPC分类号： C12N15/101 ,  B01L3/502738 ,  B01L3/502746 ,  B01L3/502761 ,  B01L3/502776 ,  B01L3/5085 ,  G01N27/44717 ,  B01L2200/0621 ,  B01L9/527

摘要： The present disclosure relates to fluidic systems and devices for processing, extracting, or purifying one or more analytes. These systems and devices can be used for processing samples and extracting nucleic acids, for example by isotachophoresis. In particular, the systems and related methods can allow for extraction of nucleic acids, including non-crosslinked nucleic acids, from samples such as tissue or cells. The systems and devices can also be used for multiplex parallel sample processing.

公开(公告)号：US11813615B2

公开(公告)日：2023-11-14

申请号：US17963377

申请日：2022-10-11

申请人： Singular Genomics Systems, Inc.

发明人： Sandor Kovacs ,  David Baranson ,  Eli N. Glezer

IPC分类号： B01L9/00

CPC分类号： B01L9/527 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2300/168 ,  B01L2300/18

摘要： The present disclosure relates to a flow cell receiver. The flow cell receiver can include at least one platen, having a plurality of ports. The flow cell receiver can include magnets. The flow cell receiver can be configured to automatically align, secure, and retain a flow cell carrier containing a flow cell.

公开(公告)号：US11788127B2

公开(公告)日：2023-10-17

申请号：US17026653

申请日：2020-09-21

申请人： Becton, Dickinson and Company

发明人： Thomas Catalino Gubatayao ,  Kalyan Handique ,  Karthik Ganesan ,  Daniel M. Drummond

IPC分类号： C12Q1/68 ,  C12Q1/686 ,  G01N21/64 ,  G01N35/00 ,  B01L7/00 ,  B01L9/00 ,  G01N35/02 ,  G01N21/27

CPC分类号： C12Q1/686 ,  B01L7/52 ,  B01L9/527 ,  G01N21/6428 ,  G01N21/6452 ,  G01N35/00693 ,  G01N35/026 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1838 ,  G01N21/274 ,  G01N21/278 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2035/00366

摘要： Systems and methods for performing simultaneous nucleic acid amplification and detection. The systems and methods comprise methods for managing a plurality of protocols in conjunction with directing a sensor array across each of a plurality of reaction chambers. In certain embodiments, the protocols comprise thermocycling profiles and the methods may introduce offsets and duration extensions into the thermocycling profiles to achieve more efficient detection behavior.

公开(公告)号：US20230311119A1

公开(公告)日：2023-10-05

申请号：US18144485

申请日：2023-05-08

申请人： ILLUMINA, INC.

发明人： Erik Williamson ,  Bryan Crane ,  Patrick Leung ,  Drew Verkade ,  Mark T. Reed

IPC分类号： B01L3/00 ,  B01L9/00 ,  G01N21/05

CPC分类号： B01L3/502715 ,  B01L9/527 ,  G01N21/05 ,  B01L7/52 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/0689 ,  B01L2300/043 ,  B01L2300/0816 ,  B01L2300/0877 ,  Y10T436/25

摘要： A fluidic device holder configured to orient a fluidic device. The device holder includes a support structure configured to receive a fluidic device. The support structure includes a base surface that faces in a direction along the Z-axis and is configured to have the fluidic device positioned thereon. The device holder also includes a plurality of reference surfaces facing in respective directions along an XY-plane. The device holder also includes an alignment assembly having an actuator and a movable locator arm that is operatively coupled to the actuator. The locator arm has an engagement end. The actuator moves the locator arm between retracted and biased positions to move the engagement end away from and toward the reference surfaces. The locator arm is configured to hold the fluidic device against the reference surfaces when the locator arm is in the biased position.