公开(公告)号：US12083521B2

公开(公告)日：2024-09-10

申请号：US16626647

申请日：2018-07-02

Applicant: BLINK AG

Inventor： Torsten Schulz ,  Eugen Ermantraut ,  Katrin Steinmetzer ,  Alrik Wolff

IPC: B01F35/513 ,  B01F23/411 ,  B01F31/20 ,  B01L3/00 ,  B01L7/00 ,  B01L99/00 ,  C12M1/00 ,  C12M1/34 ,  G01N15/00 ,  G01N15/1433 ,  G01N15/14

CPC classification number: B01L7/52 ,  B01F23/4111 ,  B01F31/20 ,  B01F35/513 ,  B01L3/502776 ,  B01L3/505 ,  C12M41/14 ,  G01N15/1433 ,  B01L2200/0636 ,  B01L2200/0684 ,  B01L2300/042 ,  B01L2300/0681 ,  B01L2300/0832 ,  B01L2300/1822 ,  B01L2400/0409 ,  B01L2400/0457 ,  B01L2400/0481 ,  B01L2400/049 ,  B01L2400/0655 ,  G01N2015/1493

Abstract: The present invention relates to a sample cartridge for incubating and/or analyzing a dispersion of particles, cells or droplets and/or for performing biochemical reactions with or in such dispersion. The present invention furthermore relates to a device for incubating a dispersion of particles, cells or droplets and/or for performing a biochemical reaction therewith. Moreover, the present invention also relates to the use of a sample cartridge or of a device for generating and/or processing a dispersion of particles, cells or droplets. Moreover, the present invention relates to a method of processing a dispersion of particles, cells or droplets. Furthermore, the present invention relates to a method of generating a dispersion of droplets and to a method of generating a dispersion of solid or semi-solid particles.

公开(公告)号：USRE50088E1

公开(公告)日：2024-08-20

申请号：US17514186

申请日：2021-11-12

Applicant: Sony Corporation

Inventor： Masataka Shinoda

IPC: G01N15/1404 ,  B01L3/00 ,  B01L3/02 ,  G01N15/14 ,  G01N15/1434 ,  G01N27/447 ,  G01N15/10 ,  G01N15/1409 ,  G01N15/149

CPC classification number: G01N15/1404 ,  B01L3/0268 ,  B01L3/502761 ,  B01L3/502776 ,  G01N15/1434 ,  G01N15/1459 ,  G01N15/1484 ,  G01N27/44743 ,  G01N27/44791 ,  B01L3/502746 ,  B01L2400/0433 ,  B01L2400/0487 ,  G01N2015/1006 ,  G01N2015/1028 ,  G01N15/1409 ,  G01N2015/1422 ,  G01N2015/1481 ,  G01N2015/1488 ,  G01N15/149 ,  Y10T137/8158

Abstract: A microchip is provided that includes a flow path through which a liquid containing a micro particle flows, an orifice through which the liquid flowing through the flow path is discharged into a space outside the microchip, and a light-irradiated portion provided at a predetermined location of the flow path and configured to be irradiated with light. A width of the flow path and a depth of the flow path at the orifice are set to be smaller than a width of the flow path and a depth of the flow path at the light-irradiated portion, and the flow path is configured to gradually decrease from upstream of the orifice in a cross-section area perpendicular to a liquid-delivering direction between the light-irradiated portion and the orifice. A cartridge including the microchip is also provided.

公开(公告)号：US20240141421A1

公开(公告)日：2024-05-02

申请号：US18384971

申请日：2023-10-30

Applicant: Stokes Bio Limited

Inventor： Mark DAVIES ,  Tara DALTON

IPC: C12Q1/686 ,  B01F25/10 ,  B01F33/30 ,  B01L3/00 ,  B01L7/00 ,  G01N35/08

CPC classification number: C12Q1/686 ,  B01F25/10 ,  B01F33/30 ,  B01L3/50273 ,  B01L3/502776 ,  B01L3/502784 ,  B01L7/525 ,  G01N35/08 ,  B01L2200/0636 ,  B01L2200/0673 ,  B01L2300/087 ,  B01L2400/0409 ,  B01L2400/0487 ,  G01N35/1095

Abstract: A microfluidic analysis system (1) performs polymerase chain reaction (PCR) analysis on a bio sample. In a centrifuge (6) the sample is separated into DNA and RNA constituents. The vortex is created by opposing flow of a silicon oil primary carrier fluid effecting circulation by viscous drag. The bio sample exits the centrifuge enveloped in the primary carrier fluid. This is pumped by a flow controller (7) to a thermal stage (9). The thermal stage (9) has a number of microfluidic devices (70) each having thermal zones (71, 72, 73) in which the bio sample is heated or cooled by heat conduction to/from a thermal carrier fluid and the primary carrier fluid. Thus, the carrier fluids envelope the sample, control its flowrate, and control its temperature without need for moving parts at the micro scale.

公开(公告)号：US11951474B2

公开(公告)日：2024-04-09

申请号：US17304452

申请日：2021-06-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan Schultz ,  David Marran

IPC: C12Q1/6869 ,  B01L3/00 ,  G01N27/447 ,  G01N35/10

CPC classification number: B01L3/502715 ,  B01L3/502 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502746 ,  B01L3/502776 ,  B01L3/52 ,  C12Q1/6869 ,  G01N27/447 ,  B01J2219/00389 ,  B01J2219/00394 ,  B01L3/502769 ,  B01L2200/16 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0874 ,  B01L2300/0877 ,  B01L2400/0487 ,  G01N35/1002 ,  G01N35/1097 ,  Y10T137/0318 ,  Y10T137/0424 ,  Y10T137/85938

Abstract: The invention provides a passive fluidics circuit for directing different fluids to a common volume, such as a reaction chamber or flow cell, without intermixing or cross contamination. The direction and rate of flow through junctions, nodes and passages of the fluidics circuit are controlled by the states of upstream valves (e.g. opened or closed), differential fluid pressures at circuit inlets or upstream reservoirs, flow path resistances, and the like. Free diffusion or leakage of fluids from unselected inlets into the common outlet or other inlets at junctions or nodes is prevented by the flow of the selected inlet fluid, a portion of which sweeps by the inlets of unselected fluids and exits the fluidics circuit by waste ports, thereby creating a barrier against undesired intermixing with the outlet flow through leakage or diffusion.

公开(公告)号：US11873173B2

公开(公告)日：2024-01-16

申请号：US18136126

申请日：2023-04-18

Applicant: CYTONOME/ST, LLC

Inventor： John R. Gilbert ,  Manish Deshpande ,  Bernard Bunner

IPC: B65G51/08 ,  B01L3/00 ,  G01N15/14 ,  B07C5/00 ,  B07C5/34 ,  B01F33/301 ,  B65G51/00 ,  B01F33/3011

CPC classification number: B65G51/08 ,  B01F33/3017 ,  B01L3/502776 ,  B07C5/00 ,  B07C5/34 ,  G01N15/1404 ,  G01N15/1484 ,  B01F33/3011 ,  B01J2219/0086 ,  B01J2219/00822 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00891 ,  B01L2200/0636 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0887 ,  B01L2400/0487 ,  B65G51/00 ,  G01N2015/1409

Abstract: A microfabricated sheath flow structure for producing a sheath flow includes a primary sheath flow channel for conveying a sheath fluid, a sample inlet for injecting a sample into the sheath fluid in the primary sheath flow channel, a primary focusing region for focusing the sample within the sheath fluid and a secondary focusing region for providing additional focusing of the sample within the sheath fluid. The secondary focusing region may be formed by a flow channel intersecting the primary sheath flow channel to inject additional sheath fluid into the primary sheath flow channel from a selected direction. A sheath flow system may comprise a plurality of sheath flow structures operating in parallel on a microfluidic chip.

公开(公告)号：US20230381781A1

公开(公告)日：2023-11-30

申请号：US18318294

申请日：2023-05-16

Applicant: Intabio, LLC

Inventor： Luc BOUSSE ,  Don Wesley ARNOLD ,  Wesley CHANG ,  Erik GENTALEN ,  Eric GWERDER ,  Scott MACK

IPC: B01L3/00 ,  G01N27/447

CPC classification number: B01L3/502776 ,  B01L2400/0427 ,  B01L3/502761 ,  G01N27/44795 ,  B01L3/5027 ,  B01L3/502715 ,  G01N27/44791 ,  B01L2200/0647 ,  B01L2300/0645 ,  B01L2300/161 ,  B01L2300/12 ,  B01L2200/0636 ,  B01L2300/047 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2200/0684 ,  B01L2400/0421 ,  B01L3/502792

Abstract: Methods, devices, and systems for performing isoelectric focusing reactions are described. The systems or devices disclosed herein may comprise fixtures that have a membrane. In some instances, the disclosed devices may be designed to perform isoelectric focusing or other separation reactions followed by further characterization of the separated analytes using mass spectrometry. Two or more isoelectric focusing reactions may be performed in parallel. The disclosed methods, devices, and systems provide for fast, accurate separation and characterization of protein analyte mixtures or other biological molecules by isoelectric point.

公开(公告)号：US11813612B2

公开(公告)日：2023-11-14

申请号：US17717800

申请日：2022-04-11

Applicant: Qorvo US, Inc.

Inventor： Ian Harmon

IPC: B01L3/00 ,  G01N29/22 ,  G01N29/24

CPC classification number: B01L3/50273 ,  B01L3/502715 ,  B01L3/502776 ,  G01N29/222 ,  G01N29/2425

Abstract: Methods include treating a portion of a sample composition to be tested for presence of an analyte by depleting or blocking the target analyte. The treated composition may be used to equilibrate an acoustic wave sensor prior to exposing the sensor to the untreated sample composition for analysis. By using the treated sample composition, in which the analyte is depleted or blocked, to equilibrate the sensor, the sensor may be equilibrated with a composition having a similar viscosity and non-specific binding characteristics to the untreated sample composition, which should result in improved accuracy when analyzing the analyte in the untreated sample composition.

公开(公告)号：US20230311123A1

公开(公告)日：2023-10-05

申请号：US18042113

申请日：2021-08-19

Applicant: Colorado State University Research Foundation

Inventor： Charles S. Henry ,  Ilhoon Jang

IPC: B01L3/00

CPC classification number: B01L3/502738 ,  B01L3/502776 ,  B01L2200/0621 ,  B01L2400/0481

Abstract: A microfluidic device includes a device body defining a microfluidic pathway including a first channel, a second channel downstream of the first channel, and a junction including a transition between the first channel and the second channel. The transition is configured to inhibit fluid entering the transition from the first channel from forming a meniscus across the second channel, thereby inhibiting capillary-driven flow into the second channel. The microfluidic device further includes a valve that, when activated while capillary-driven flow of the fluid is inhibited at the transition, induces capillary-driven flow through the second channel by facilitating formation of the meniscus.

公开(公告)号：US20230271793A1

公开(公告)日：2023-08-31

申请号：US18136126

申请日：2023-04-18

Applicant: CYTONOME/ST, LLC

Inventor： John R. Gilbert ,  Manish Deshpande ,  Bernard Bunner

IPC: B65G51/08 ,  B01L3/00 ,  G01N15/14 ,  B07C5/00 ,  B07C5/34 ,  B01F33/301

CPC classification number: B65G51/08 ,  B01L3/502776 ,  G01N15/1404 ,  G01N15/1484 ,  B07C5/00 ,  B07C5/34 ,  B01F33/3017 ,  B01J2219/00822 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/0086 ,  B01J2219/00891 ,  B01L2200/0636 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2400/0487 ,  B65G51/00

Abstract: A microfabricated sheath flow structure for producing a sheath flow includes a primary sheath flow channel for conveying a sheath fluid, a sample inlet for injecting a sample into the sheath fluid in the primary sheath flow channel, a primary focusing region for focusing the sample within the sheath fluid and a secondary focusing region for providing additional focusing of the sample within the sheath fluid. The secondary focusing region may be formed by a flow channel intersecting the primary sheath flow channel to inject additional sheath fluid into the primary sheath flow channel from a selected direction. A sheath flow system may comprise a plurality of sheath flow structures operating in parallel on a microfluidic chip.

公开(公告)号：US20190240663A1

公开(公告)日：2019-08-08

申请号：US16315732

申请日：2017-07-07

Applicant: Vanderbilt University

Inventor： Sinead E. MILLER ,  Charleson S. BELL ,  Todd D. GIORGIO ,  Andrew L. COOK

IPC: B01L3/00 ,  A61M5/165 ,  A61K38/14 ,  A61K38/12

CPC classification number: B01L3/502753 ,  A61K38/12 ,  A61K38/14 ,  A61M5/165 ,  B01L3/502715 ,  B01L3/502776 ,  B01L2300/0861 ,  B01L2300/16 ,  G01N33/48 ,  G01N33/53 ,  G01N33/543

Abstract: The present disclosure relates to a fluidic device to detect, capture, and/or remove disease material in a biological fluid. The present invention also relates to methods for the treatment/prevention of sepsis through the use of the claimed device.