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公开(公告)号：US20240142368A1
公开(公告)日：2024-05-02
申请号：US18384641
申请日：2023-10-27
Applicant: CYTONOME/ST, LLC
Inventor： Gary Durack , William J. Williams, V
IPC: G01N15/14
CPC classification number: G01N15/1434 , G01N15/1429 , G01N2015/1452
Abstract: A method, system and apparatus for selecting cells is disclosed. An example embodiment provides spatial information denoting the location, on a four part array of sensors, referred to as a quad array, of the center, of each particle that passes through the focused laser beam in a cell sorter. The center of a particle is commonly determined by the average fluorescence intensity within the field of view of a Side Fluorescence (SFL) collection lens. Fluorescence collected by an SFL lens from a particle having any orientation and any position in the core stream produces a spot large enough to illuminate all four segments of a quad array. By independently measuring the fluorescence intensity recorded by each of the four array segments for a particle, it is possible to calculate the x-y coordinate position across the quad array segments of the average total fluorescence emission collected from that particle.
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2.发明公开公开(公告)号：US20240042491A1
公开(公告)日：2024-02-08
申请号：US18382268
申请日：2023-10-20
Applicant: CYTONOME/ST, LLC
Inventor： Gary Durack , William J. Williams, V
IPC: B07C5/342
CPC classification number: B07C5/3425 , B07C5/362
Abstract: Systems and methods for particle sorting are presented including a monitoring system downstream of a particle separator or sorter. The system can utilize the monitoring system to adjust or calibrate operational parameters of the system in real time. When a particle of interest is mis-sorted, the probability is high that the particle of interest has been sorted into a non-targeted sortable unit that was adjacent in sequence to the sortable unit that was expected to include the particle of interest. The monitoring system monitors non-targeted sortable units in the system that were adjacent in sequence to targeted sortable units that are predicted to contain particles of interest. Signals from the monitoring system enable automated adjustment or calibration of operational parameters of the system such as sort delay or purity mask parameters.
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公开(公告)号：US20230211310A1
公开(公告)日：2023-07-06
申请号：US18120694
申请日：2023-03-13
Applicant: CYTONOME/ST, LLC
Inventor： Johnathan Charles Sharpe , Donald Francis Perrault, JR.
CPC classification number: B01J19/0093 , B01L3/502715 , G01N15/1404 , B01J2219/0097 , B01J2219/00826 , B01J2219/00891 , B01J2219/00934 , B01L2300/08
Abstract: Systems and methods taught herein enable simultaneous forward and side detection of light originating within a microfluidic channel disposed in a substrate. At least a portion of the microfluidic channel is located in the substrate relative to a first side surface of the substrate to enable simultaneous detection paths with respect to extinction (i.e., 0°) and side detection (i.e., 90°). The location of the microfluidic channel as taught herein enables a maximal half-angle for a ray of light passing from a center of the portion of the microfluidic channel through the first side surface to be in a range from 25 to 90 degrees in some embodiments. By placing at least the portion of the microfluidic channel proximate to the side surface of the substrate, a significantly greater proportion of light emitted or scattered from a particle within the microfluidic channel can be collected and imaged on a detector as compared to conventional particle processing chips.
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公开(公告)号：US20210039105A1
公开(公告)日：2021-02-11
申请号：US16989570
申请日：2020-08-10
Applicant: CYTONOME/ST, LLC
Inventor： Kiryakos S. Mutafopulos , Johnathan Charles Sharpe
Abstract: This relates to acoustic microfluidic systems that can generate emulsions/droplets or encapsulate particles of interest (including mammalian cells, bacteria cells, or other cells) into droplets upon detection of the particles of interest flowing in a stream of particles. The systems operate on the detect/decide/deflect principle wherein the deflection step, in a single operation, not only deflects particles of interest from a stream of particles but also encapsulates the particles of interest in an emulsion droplet. The microfluidic systems have an abrupt transition in the channel geometry from a shorter channel to a taller channel (i.e., in the shape of a ‘step’) to break the stream of the dispersed phase into a droplet upon acoustic actuation. When there is no acoustic wave present, no droplets/emulsions are generated and the stream of particles proceeds uninterrupted. The rapid actuation and post-actuation recovery employed by the microfluidic systems taught herein ensure that the vast majority of selected particles are properly deflected, that few or no empty droplets are produced, and that total throughput remains high.
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公开(公告)号：US20200240897A1
公开(公告)日：2020-07-30
申请号：US16721431
申请日：2019-12-19
Applicant: CYTONOME/ST, LLC
Inventor： John R. Gilbert , Edward Sinofsky , Manish Deshpande
Abstract: An optical system for acquiring fast spectra from spatially channel arrays includes a light source for producing a light beam that passes through the microfluidic chip or the channel to be monitored, one or more lenses or optical fibers for capturing the light from the light source after interaction with the particles or chemicals in the microfluidic channels, and one or more detectors. The detectors, which may include light amplifying elements, detect each light signal and transducer the light signal into an electronic signal. The electronic signals, each representing the intensity of an optical signal, pass from each detector to an electronic data acquisition system for analysis. The light amplifying element or elements may comprise an array of phototubes, a multianode phototube, or a multichannel plate based image intensifier coupled to an array of photodiode detectors.
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Patent Agency Ranking
公开(公告)号：US20200122937A1
公开(公告)日：2020-04-23
申请号：US16719537
申请日：2019-12-18
Applicant: CYTONOME/ST, LLC
Inventor： John R. Gilbert , Manish Deshpande , Bernard Bunner
Abstract: A microfabricated sheath flow structure for producing a sheath flow includes a primary sheath flow channel for conveying a sheath fluid, a sample inlet for injecting a sample into the sheath fluid in the primary sheath flow channel, a primary focusing region for focusing the sample within the sheath fluid and a secondary focusing region for providing additional focusing of the sample within the sheath fluid. The secondary focusing region may be formed by a flow channel intersecting the primary sheath flow channel to inject additional sheath fluid into the primary sheath flow channel from a selected direction. A sheath flow system may comprise a plurality of sheath flow structures operating in parallel on a microfluidic chip.
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公开(公告)号：US10543992B2
公开(公告)日：2020-01-28
申请号：US15797790
申请日：2017-10-30
Applicant: CYTONOME/ST, LLC
Inventor： John R. Gilbert , Manish Deshpande , Bernard Bunner
Abstract: A microfabricated sheath flow structure for producing a sheath flow includes a primary sheath flow channel for conveying a sheath fluid, a sample inlet for injecting a sample into the sheath fluid in the primary sheath flow channel, a primary focusing region for focusing the sample within the sheath fluid and a secondary focusing region for providing additional focusing of the sample within the sheath fluid. The secondary focusing region may be formed by a flow channel intersecting the primary sheath flow channel to inject additional sheath fluid into the primary sheath flow channel from a selected direction. A sheath flow system may comprise a plurality of sheath flow structures operating in parallel on a microfluidic chip.
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公开(公告)号：US10274414B2
公开(公告)日：2019-04-30
申请号：US14281353
申请日：2014-05-19
Applicant: CYTONOME/ST, LLC
Abstract: Systems, methods and non-transitory storage medium are disclosed herein for adjusting an output of a particle inspection system representative of a particle characteristic for a particle flowing in a flow-path of a particle processing system. More particularly, the output may be processed and a calibrated output of the particle characteristic generated. In other embodiments, one or more calibration particles are used. Thus, an output of a particle inspection system representative of a particle characteristic for one or more calibration particles flowing in a flow-path of a particle processing system may be compared relative to a standard and an action may be taken based on a result of the comparing the output to the standard.
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9.发明申请公开(公告)号：US20180221879A1
公开(公告)日：2018-08-09
申请号：US15941793
申请日：2018-03-30
Applicant: CYTONOME/ST, LLC
Inventor： John R. Gilbert , Sebastian Böhm , Manish Deshpande
CPC classification number: B01L3/502746 , B01L3/502715 , B01L3/502738 , B01L2200/0605 , B01L2300/0816 , B01L2300/0858 , B01L2400/06 , B01L2400/082 , F16K99/0019 , F16K99/0061 , F16K2099/0084
Abstract: A microfluidic device includes a microchannel having an interior bounded by a side wall, an inlet, a switching region, and a plurality of outlet channels downstream of the switching region. The microchannel is formed in a microfluidic chip substrate and configured to accommodate a flow of liquid through the microchannel. The microfluidic device includes a valve operatively coupled to the switching region comprising a sealed reservoir. A side passage extends between the reservoir and the interior of the microchannel via an aperture in the side wall and is configured to accommodate a volume of liquid between the interior of the microchannel and the reservoir. The microfluidic device includes an actuator integrated into the microfluidic chip and configured to increase an internal pressure of the reservoir and move at least a portion of the volume of the liquid from the side passage into the microchannel to deflect a portion of the liquid flowing through the microchannel.
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公开(公告)号：US20180208412A1
公开(公告)日：2018-07-26
申请号：US15797790
申请日：2017-10-30
Applicant: CYTONOME/ST, LLC
Inventor： John R. Gilbert , Manish Deshpande , Bernard Bunner
CPC classification number: B65G51/08 , B01F13/0062 , B01F13/0064 , B01J2219/00822 , B01J2219/00831 , B01J2219/00833 , B01J2219/0086 , B01J2219/00891 , B01L3/502776 , B01L2200/0636 , B01L2200/0673 , B01L2300/0816 , B01L2300/0864 , B01L2300/0887 , B01L2400/0487 , B65G51/00 , G01N15/1404 , G01N15/1484 , G01N2015/1409
Abstract: A microfabricated sheath flow structure for producing a sheath flow includes a primary sheath flow channel for conveying a sheath fluid, a sample inlet for injecting a sample into the sheath fluid in the primary sheath flow channel, a primary focusing region for focusing the sample within the sheath fluid and a secondary focusing region for providing additional focusing of the sample within the sheath fluid. The secondary focusing region may be formed by a flow channel intersecting the primary sheath flow channel to inject additional sheath fluid into the primary sheath flow channel from a selected direction. A sheath flow system may comprise a plurality of sheath flow structures operating in parallel on a microfluidic chip.
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