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    Physiology and pathophysiology of human gut: intestine-on-chip
    1.
    发明授权
    Physiology and pathophysiology of human gut: intestine-on-chip 有权

    公开(公告)号：US11566231B2

    公开(公告)日：2023-01-31

    申请号：US16825213

    申请日：2020-03-20

    申请人： EMULATE, INC.

    发明人： Athanasia Apostolou ,  Antonio Varone ,  Magdalena Kasendra ,  Raymond Luc

    IPC分类号： C12N5/071 ,  C12M3/06 ,  C12M1/12 ,  G01N1/30

    摘要： An in vitro microfluidic intestine on-chip is described herein that mimics the structure and at least one function of specific areas of the gastrointestinal system in vivo. In particular, a multicellular, layered, microfluidic intestinal cell culture, which is some embodiments is derived from patient's enteroids-derived cells, is described comprising L cells, allowing for interactions between L cells and gastrointestinal epithelial cells, endothelial cells and immune cells. This in vitro microfluidic system can be used for modeling inflammatory gastrointestinal autoimmune tissue, e.g., diabetes, obesity, intestinal insufficiency and other inflammatory gastrointestinal disorders. These multicellular-layered microfluidic intestine on-chips further allow for comparisons between types of gastrointestinal tissues, e.g., small intestinal duodenum, small intestinal jejunum, small intestinal ileum, large intestinal colon, etc., and between disease states of gastrointestinal tissue, i.e. healthy, pre-disease and diseased areas. Additionally, these microfluidic gut-on-chips allow identification of cells and cellular derived factors driving disease states and drug testing for reducing inflammation.

    IN VITRO EPITHELIAL MODELS COMPRISING LAMINA PROPRIA-DERIVED CELLS
    4.
    发明申请
    IN VITRO EPITHELIAL MODELS COMPRISING LAMINA PROPRIA-DERIVED CELLS 有权

    公开(公告)号：US20210031197A1

    公开(公告)日：2021-02-04

    申请号：US17024221

    申请日：2020-09-17

    申请人： EMULATE, Inc.

    发明人： S. Jordan Kerns ,  Riccardo Barrile ,  Geraldine Hamilton ,  Catherine Karalis ,  Daniel Levner ,  Carolina Lucchesi ,  Antonio Varone ,  Remi Villenave

    IPC分类号： B01L3/00 ,  C12M1/00 ,  C12M3/06 ,  C12M1/42 ,  G01N33/50

    摘要： An in vitro microfluidic “organ-on-chip” is described herein that mimics the structure and at least one function of specific areas of the epithelial system in vivo. In particular, a multicellular, layered, microfluidic culture is described, allowing for interactions between lamina propria-derived cells and the associated tissue specific epithelial cells and endothelial cells. This in vitro microfluidic system can be used for modeling inflammatory tissue, e.g., autoimmune disorders involving epithelia and diseases involving epithelial layers. These multicellular, layered microfluidic “organ-on-chip”, e.g. “epithelia-on-chip” further allow for comparisons between types of epithelia tissues, e.g., lung (Lung-On-Chip), bronchial (Airway-On-Chip), skin (Skin-On-Chip), cervix (Cervix-On-Chip), blood brain barrier (BBB-On-Chip), etc., in additional to neurovascular tissue, (Brain-On-Chip), and between different disease states of tissue, i.e. healthy, pre-disease and diseased areas. Additionally, these microfluidic “organ-on-chips” allow identification of cells and cellular derived factors driving disease states in addition to drug testing for reducing inflammation effecting epithelial regions.