公开(公告)号：US20240353395A1

公开(公告)日：2024-10-24

申请号：US18640027

申请日：2024-04-19

申请人： Plexision

发明人： Rakesh Sindhi ,  Chethan Ashokkumar

IPC分类号： G01N33/50

CPC分类号： G01N33/5047 ,  G01N2333/015 ,  G01N2333/165 ,  G01N2333/70575 ,  G01N2800/50 ,  G01N2800/52

摘要： A method for measuring cell-mediated immunity to AAV-related gene therapy is provided. The method includes measuring cell-mediated immunity to adeno-associated viruses (AAV) and the transgene carried by these viruses which are being used for gene replacement therapy to treat genetic disorders. These measurements are used to a) estimate the risk of failure, b) the adequacy of immunosuppression, c) adjustment of immunosuppression d) to achieve desired suppression of the immune response, and e) to determine whether a patient about to undergo gene therapy is likely to develop and immune response to the therapy or not. The related embodiment which measures several complement or related proteins on cells is aimed at measuring the risk of TMA, its severity and its response to treatment. The present invention also provides a kit for measuring cell mediated immunity to AAV gene therapy.

公开(公告)号：US20240310361A1

公开(公告)日：2024-09-19

申请号：US18605551

申请日：2024-03-14

申请人： CANON KABUSHIKI KAISHA

发明人： KOUICHI KATO ,  TAKANORI YODA ,  TSUTOMU HONMA

IPC分类号： G01N33/50 ,  G01N21/64

CPC分类号： G01N33/5047 ,  G01N21/6428 ,  G01N2021/6439

摘要： Provided is a method of evaluating the immune response of a cell group to a test substance, the method comprising the steps of: immobilizing a cell membrane modifier comprising a polymer having a hydrophobic chain and a hydrophilic chain on a substrate; introducing a calcium fluorescent indicator into the cell group; immobilizing the cell group on the substrate through the cell membrane modifier, adding the test substance to the cell group; and detecting the fluorescence of the cell group derived from the calcium fluorescent indicator with a detector.

公开(公告)号：US20240310360A1

公开(公告)日：2024-09-19

申请号：US18605531

申请日：2024-03-14

申请人： CANON KABUSHIKI KAISHA

发明人： KOUICHI KATO ,  TSUTOMU HONMA ,  TAKANORI YODA

IPC分类号： G01N33/50 ,  G01N21/64 ,  G06T7/00

CPC分类号： G01N33/5047 ,  G01N21/6486 ,  G06T7/0014 ,  G06T2207/10024 ,  G06T2207/10064 ,  G06T2207/30024

摘要： A method of evaluating an immune response of a cell group to a test substance, the method comprising evaluating the immune response of the cell group to the test substance based on a comparison between autofluorescence information of a first sample prepared by adding the test substance to the cell group and autofluorescence information of a second sample, the second sample serving as a control for the first sample.

公开(公告)号：US20240301358A1

公开(公告)日：2024-09-12

申请号：US18602727

申请日：2024-03-12

申请人： EMULATE, Inc.

发明人： Athanasia Apostolou ,  Antonio Varone ,  Magdalena Kasendra ,  Raymond Luc

IPC分类号： C12N5/071 ,  B01L3/00 ,  C12M1/00 ,  C12M1/12 ,  C12M3/06 ,  G01N1/30 ,  G01N33/50

CPC分类号： C12N5/0679 ,  B01L3/502715 ,  B01L3/502761 ,  C12M23/16 ,  C12M23/26 ,  C12M25/02 ,  C12N5/069 ,  G01N1/30 ,  G01N33/5047 ,  G01N33/5064 ,  B01L2200/16 ,  B01L2300/123 ,  B01L2300/16 ,  C12N2500/00 ,  C12N2501/052 ,  C12N2501/2301 ,  C12N2501/2306 ,  C12N2501/25

摘要： An in vitro microfluidic intestine on-chip is described herein that mimics the structure and at least one function of specific areas of the gastrointestinal system in vivo. In particular, a multicellular, layered, microfluidic intestinal cell culture, which is some embodiments is derived from patient's enteroids-derived cells, is described comprising L cells, allowing for interactions between L cells and gastrointestinal epithelial cells, endothelial cells and immune cells. This in vitro microfluidic system can be used for modeling inflammatory gastrointestinal autoimmune tissue, e.g., diabetes, obesity, intestinal insufficiency and other inflammatory gastrointestinal disorders. These multicellular-layered microfluidic intestine on-chips further allow for comparisons between types of gastrointestinal tissues, e.g., small intestinal duodenum, small intestinal jejunum, small intestinal ileum, large intestinal colon, etc., and between disease states of gastrointestinal tissue, i.e. healthy, pre-disease and diseased areas. Additionally, these microfluidic gut-on-chips allow identification of cells and cellular derived factors driving disease states and drug testing for reducing inflammation.

公开(公告)号：US12018284B2

公开(公告)日：2024-06-25

申请号：US17984023

申请日：2022-11-09

申请人： EMULATE, INC.

发明人： Athanasia Apostolou ,  Antonio Varone ,  Magdalena Kasendra ,  Raymond Luc

IPC分类号： C12N5/071 ,  B01L3/00 ,  C12M1/00 ,  C12M1/12 ,  C12M3/06 ,  G01N1/30 ,  G01N33/50

CPC分类号： C12N5/0679 ,  B01L3/502715 ,  B01L3/502761 ,  C12M23/16 ,  C12M23/26 ,  C12M25/02 ,  C12N5/069 ,  G01N1/30 ,  G01N33/5047 ,  G01N33/5064 ,  B01L2200/16 ,  B01L2300/123 ,  B01L2300/16 ,  C12N2500/00 ,  C12N2501/052 ,  C12N2501/2301 ,  C12N2501/2306 ,  C12N2501/25

摘要： An in vitro microfluidic intestine on-chip is described herein that mimics the structure and at least one function of specific areas of the gastrointestinal system in vivo. In particular, a multicellular, layered, microfluidic intestinal cell culture, which is some embodiments is derived from patient's enteroids-derived cells, is described comprising L cells, allowing for interactions between L cells and gastrointestinal epithelial cells, endothelial cells and immune cells. This in vitro microfluidic system can be used for modeling inflammatory gastrointestinal autoimmune tissue, e.g., diabetes, obesity, intestinal insufficiency and other inflammatory gastrointestinal disorders. These multicellular-layered microfluidic intestine on-chips further allow for comparisons between types of gastrointestinal tissues, e.g., small intestinal duodenum, small intestinal jejunum, small intestinal ileum, large intestinal colon, etc., and between disease states of gastrointestinal tissue, i.e. healthy, pre-disease and diseased areas. Additionally, these microfluidic gut-on-chips allow identification of cells and cellular derived factors driving disease states and drug testing for reducing inflammation.

公开(公告)号：US11998914B2

公开(公告)日：2024-06-04

申请号：US17656244

申请日：2022-03-24

申请人： Berkeley Lights, Inc.

发明人： Kevin T. Chapman ,  Daniele Malleo ,  J. Tanner Nevill ,  Steven W. Short ,  Mark P. White ,  M. Jimena Loureiro

IPC分类号： B01L3/00 ,  B03C5/00 ,  B03C5/02 ,  G01N15/14 ,  G01N33/50 ,  G01N33/543 ,  G01N33/68

CPC分类号： B01L3/502761 ,  B03C5/005 ,  B03C5/026 ,  G01N15/1484 ,  G01N33/5023 ,  G01N33/5047 ,  G01N33/505 ,  G01N33/5052 ,  G01N33/54313 ,  G01N33/6854 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2400/0454 ,  B03C2201/26

摘要： Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.

公开(公告)号：US20240158465A1

公开(公告)日：2024-05-16

申请号：US18517066

申请日：2023-11-22

申请人： GEORGE MASON UNIVERSITY

发明人： Caroline Dieckmann Hoemann ,  Julia Alexa Leonard ,  Virginia A. Espina ,  David Kepplinger

IPC分类号： C07K14/705 ,  C12N5/0787 ,  G01N33/50 ,  G01N33/68

CPC分类号： C07K14/7056 ,  C12N5/0642 ,  G01N33/5047 ,  G01N33/6893 ,  C12N2501/052 ,  C12N2501/90 ,  C12N2501/999 ,  C12N2523/00 ,  G01N2333/8125

摘要： In an embodiment, present invention relates to a method of generating, ex vivo production of soluble Lox-1 (sLox-1), comprising: introducing a sample containing blood into a device; adding a coagulation enhancing material in the sample to form a cultured blood clot; incubating the cultured blood clot in the device at a temperature greater than 25° C. and less than 45° C. for at least 2 hours to allow production of Lox-1 from neutrophils of blood and to shed the sLox-1 outside the cultured blood clot; and collecting sLox-1 shedded in the device, wherein the method is configured to shed sLox-1 more than fresh blood.

公开(公告)号：US11980620B2

公开(公告)日：2024-05-14

申请号：US16620064

申请日：2017-06-08

申请人： THE CHILDREN'S MEDICAL CENTER CORPORATION

发明人： Sergei Doulatov ,  George Q. Daley

IPC分类号： A61K31/517 ,  A61P3/00 ,  A61P7/06 ,  C12N5/0789 ,  C12N15/86 ,  G01N33/50 ,  A61K45/06

CPC分类号： A61K31/517 ,  A61P3/00 ,  A61P7/06 ,  C12N5/0647 ,  C12N15/86 ,  G01N33/5047 ,  A61K45/06

摘要： The present invention relates generally to methods for treatment of ribosomal disorders and ribosomopathy, e.g. Diamond Blackfan anemia (DBA). In some embodiments, the invention relates to methods for the use of a small-molecule autophagy modulator for treatment of ribosomal disorders and ribosomopathy. The invention also relates to small molecule drug discovery and methods of screening compositions to determine their effectiveness for treatment of ribosomal disorders and ribosomopathies.

公开(公告)号：US20240125772A1

公开(公告)日：2024-04-18

申请号：US18503564

申请日：2023-11-07

申请人： 10x Genomics, Inc.

发明人： Joshua Delaney ,  Shalini Gohil ,  Jill Herschleb ,  Adam Lowe ,  Albert Kim ,  Meiliana Tjandra

IPC分类号： G01N33/53 ,  C07D213/04 ,  C07D239/24 ,  C12N9/48 ,  G01N33/50

CPC分类号： G01N33/5308 ,  C07D213/04 ,  C07D239/24 ,  C12N9/48 ,  G01N33/5047 ,  G01N2001/307 ,  G01N2333/948

摘要： The present disclosure provides compositions and methods for using fixed biological samples in partition-based assays. In at least one embodiment, the disclosure provides a composition comprising a fixed biological sample and an un-fixing agent contained in a partition, such as a discrete droplet. In some embodiments, the disclosure provides un-fixing agent compounds capable of catalytically cleaving crosslinks in fixed biological samples, particularly crosslinked nucleic acids, such as RNA.

公开(公告)号：US20240109939A1

公开(公告)日：2024-04-04

申请号：US18272779

申请日：2022-01-26

申请人： Oxford Immunotec Limited

发明人： Daniel Cochrane

IPC分类号： C07K14/005 ,  G01N33/50

CPC分类号： C07K14/005 ,  G01N33/5047 ,  G01N2333/165

摘要： The disclosure concerns a method for producing a pool of fragments derived from a microbial protein. The disclosure also concerns a pool of fragments derived from a microbial protein, and a method for determining the presence or absence of immune cells targeting a microbe.