公开(公告)号：US20240361217A1

公开(公告)日：2024-10-31

申请号：US18770727

申请日：2024-07-12

申请人： Phaim Pharma Ltd.

发明人： Tihamer ORBAN ,  Jalahej HEYMAN ,  Nara DAUBENEY ,  Piers DAUBENEY

IPC分类号： G01N1/40 ,  A61K35/17 ,  A61P17/06 ,  A61P37/06 ,  C12N5/0783 ,  C12Q1/02 ,  G01N1/06 ,  G01N1/28 ,  G01N1/36 ,  G01N1/42 ,  G01N33/50

CPC分类号： G01N1/4077 ,  A61K35/17 ,  A61P17/06 ,  A61P37/06 ,  C12N5/0636 ,  C12Q1/02 ,  G01N1/06 ,  G01N1/2813 ,  G01N1/286 ,  G01N1/36 ,  G01N1/42 ,  G01N33/505 ,  G01N33/5091 ,  G01N2001/2873 ,  G01N2800/24 ,  G01N2800/56

摘要： Provided are methods of preparing an immune cell sample from a subject having an autoimmune disorder, the method comprising: obtaining a tissue sample from the subject; and isolating a single immune cell in situ from the tissue sample using laser capture microdissection.

公开(公告)号：US20240352481A1

公开(公告)日：2024-10-24

申请号：US18292590

申请日：2022-07-29

申请人： VOR BIOPHARMA INC.

发明人： Brikena Gjeci ,  Tirtha Chakraborty ,  Julian Scherer

IPC分类号： C12N15/86 ,  C12N5/0783 ,  C12Q1/6897 ,  G01N33/50

CPC分类号： C12N15/86 ,  C12N5/0636 ,  C12Q1/6897 ,  G01N33/505 ,  C12N2510/00 ,  C12N2740/16043 ,  C12N2830/001 ,  C12N2830/15 ,  C12N2840/20

摘要： Provided herein are IL-2 reporter systems comprising nucleic acid constructs comprising a nucleotide sequence encoding a reporter molecule operably linked to a minimal nuclear factor of activated T cells (NFAT)-responsive promoter. Also provided herein are vectors, cells, and cell lines comprising such nucleic acids. Also provided herein are methods of making and using such cells, for example to measure the ability of a chimeric antigen receptor (CAR) to induce nuclear factor of activated T cells (NFAT)-signaling in a cell.

公开(公告)号：US20240342704A1

公开(公告)日：2024-10-17

申请号：US18505857

申请日：2023-11-09

申请人： Bruker Cellular Analysis, Inc.

发明人： Peter J. Beemiller ,  Alexander J. Mastroianni ,  Shao Ning Pei ,  Randall D. Lowe, Jr. ,  Annamaria Mocciaro ,  Kevin D. Loutherback ,  Yelena Bronevetsky ,  Guido K. Stadler ,  Andrew W. McFarland ,  Kevin T. Chapman ,  Duane Smith ,  Natalie C. Marks ,  Amanda L. Goodsell

IPC分类号： B01L3/00 ,  C07K14/74 ,  G01N33/50 ,  G01N33/68

CPC分类号： B01L3/5027 ,  C07K14/70539 ,  G01N33/5008 ,  G01N33/6845 ,  B01L2300/16 ,  G01N33/505 ,  G01N2333/70539

摘要： Proto-antigen-presenting surfaces and related kits, methods, and uses are provided. The proto-antigen-presenting surface can comprise a plurality of primary activating molecular ligands comprising a major histocompatibility complex (MHC) molecule configured to bind to a T cell receptor (TCR) of a T cell and a plurality of co-activating molecular ligands each including a TCR co-activating molecule or an adjunct TCR activating molecule, wherein an exchange factor is bound to the MHC molecules. Exchange factors include, e.g., dipeptides such as GL, GF, GR, etc. Proto-antigen-presenting surfaces can be used to rapidly prepare antigen-presenting surfaces comprising one or more peptide antigens of interest by contacting the proto-antigen-presenting surface with one or more peptide antigens so as to displace the exchange factor. As such, the disclosure facilitates rapid evaluation of the immunogenicity of peptide antigens for activating T lymphocytes.

公开(公告)号：US20240319172A1

公开(公告)日：2024-09-26

申请号：US18573079

申请日：2022-06-22

申请人： INSTIL BIO, INC.

发明人： Sunetra BISWAS

IPC分类号： G01N33/50 ,  C12N5/0781 ,  C12N5/0783 ,  G01N33/58 ,  G01N33/68

CPC分类号： G01N33/505 ,  C12N5/0635 ,  C12N5/0636 ,  G01N33/582 ,  G01N33/6854 ,  C12N2501/515 ,  G01N2333/57 ,  G01N2333/70596

摘要： The subject matter described herein is directed to methods for determining the potency of isolated and expanded tumor infiltrating lymphocytes (TILs) and producing therapeutic populations of TILs, and compositions involving the same and methods of treatment involving the same.

公开(公告)号：US12099053B2

公开(公告)日：2024-09-24

申请号：US17563701

申请日：2021-12-28

申请人： INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE

发明人： Hsiang-Chun Wei ,  Chih-Hsiang Liu ,  Chung-Lun Kuo ,  Chun-Wei Lo ,  Chia-Hung Cho ,  Wei-Hsiung Tsai

IPC分类号： G01N33/50 ,  G01N15/1433 ,  G03H1/00 ,  G03H1/04 ,  G03H1/08 ,  G06T7/00

CPC分类号： G01N33/505 ,  G01N15/1433 ,  G03H1/0005 ,  G03H1/0443 ,  G03H1/0866 ,  G06T7/0012 ,  G03H2001/0033 ,  G03H2001/005 ,  G06T2207/10056 ,  G06T2207/10064 ,  G06T2207/20081 ,  G06T2207/20084 ,  G06T2207/30024

摘要： A method of training AI for label-free cell viability determination includes a step of providing a cell sample, a step of obtaining a fluorescence image and a DHM image of the cell sample, a step of determining a first cell viability of the cell sample according to the fluorescence image of the cell sample, a step of labeling the DHM image of the cell sample as a model specifying the first cell viability, and a step of performing AI training by using the model containing the DHM image of the cell sample.

公开(公告)号：US20240307462A1

公开(公告)日：2024-09-19

申请号：US18676470

申请日：2024-05-28

申请人： The University of Tokyo

发明人： Kenya Honda ,  Koji Atarashi ,  Kikuji Itoh ,  Takeshi Tanoue

IPC分类号： A61K35/742 ,  A01K67/0275 ,  A61K9/00 ,  A61K9/48 ,  A61K35/00 ,  A61K35/74 ,  A61K39/00 ,  A61K39/08 ,  A61K39/39 ,  A61K45/00 ,  A61K45/06 ,  C12Q1/689 ,  G01N33/50

CPC分类号： A61K35/742 ,  A01K67/0275 ,  A61K9/0053 ,  A61K9/48 ,  A61K35/74 ,  A61K39/0008 ,  A61K39/08 ,  A61K39/39 ,  A61K45/00 ,  A61K45/06 ,  C12Q1/689 ,  G01N33/505 ,  A01K2267/0325 ,  A61K35/00 ,  A61K2039/52 ,  A61K2039/542 ,  A61K2039/55594 ,  A61K2039/57 ,  C12Q2600/158 ,  G01N2333/33 ,  G01N2500/10

摘要： It was found that bacteria belonging to the genus Clostridium induce accumulation of regulatory T cells (Treg cells) in the colon. Moreover, the present inventors found that regulatory T cells (Treg cells) induced by from these bacteria suppressed proliferation of effector T-cells. From these findings, the present inventors found that the use of bacteria belonging to the genus Clostridium or a physiologically active substance derived therefrom made it possible to induce proliferation or accumulation of regulatory T cells (Treg cells), and further to suppress immune functions.

公开(公告)号：US20240301055A1

公开(公告)日：2024-09-12

申请号：US18357533

申请日：2023-07-24

申请人： IMMUTEP ,  INSERM - INSTITUT NATIONAL DELA SANTE ET DE LA RECHERCHE MEDICALE

发明人： Thomas HAUDEBOURG ,  Frederic TRIEBEL ,  Bernard VANHOVE

IPC分类号： C07K16/28 ,  A61K39/00 ,  G01N33/50

CPC分类号： C07K16/2803 ,  G01N33/505 ,  A61K2039/505 ,  C07K2317/24 ,  C07K2317/565 ,  C07K2317/732 ,  C07K2317/734 ,  C07K2317/92 ,  G01N2333/70503

摘要： Cytotoxic anti-LAG-3 monoclonal antibodies or fragments thereof causing depletion of LAG-3+ activated T cells are described, as are related pharmaceuticals and methods of treating. Also described are related nucleic acid and protein sequences.

公开(公告)号：US12064465B2

公开(公告)日：2024-08-20

申请号：US17491742

申请日：2021-10-01

申请人： Immatics Biotechnologies GmbH

发明人： Jens Fritsche ,  Toni Weinschenk ,  Steffen Walter ,  Peter Lewandrowski ,  Harpreet Singh

IPC分类号： A61P35/00 ,  A61K38/17 ,  A61K39/00 ,  C07K7/06 ,  G01N33/50

CPC分类号： A61K38/1709 ,  A61K39/0011 ,  A61P35/00 ,  C07K7/06 ,  G01N33/505 ,  A61K2039/5158 ,  A61K2039/572 ,  A61K2039/585 ,  C07K2319/00

摘要： A pharmaceutical composition contains an antibody or a fragment thereof specific for COL6A3 for the treatment of a cancer. A method of treating a cancer includes administering to a subject in need thereof the pharmaceutical composition. A kit includes a container that contains the pharmaceutical composition. A method of producing an antibody or a fragment thereof against a peptide or a MHC/peptide complex. A method for detecting a diseased tissue includes administering to a subject in need thereof an antibody or a fragment thereof conjugated to a radioisotope and detecting a signal from the radioisotope in the subject. A method for treating a diseased tissue includes administering to a subject in need thereof an antibody or a fragment thereof conjugated to a toxin.

公开(公告)号：US20240264151A1

公开(公告)日：2024-08-08

申请号：US18403429

申请日：2024-01-03

申请人： THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA

发明人： E. John WHERRY ,  Bertram BENGSCH ,  Omar KHAN ,  Jennifer WU ,  Josephine GILES

IPC分类号： G01N33/50 ,  C12Q1/686 ,  C12Q1/6869

CPC分类号： G01N33/505 ,  C12Q1/686 ,  C12Q1/6869 ,  G01N33/5091 ,  C12Q2600/118 ,  G01N2800/52

摘要： The present invention provides compositions and methods for detecting exhausted T cells in a subject. The present invention also provides methods for treating a subject having a disease characterized by the presence of exhausted T cells or certain subpopulations thereof.

公开(公告)号：US11998914B2

公开(公告)日：2024-06-04

申请号：US17656244

申请日：2022-03-24

申请人： Berkeley Lights, Inc.

发明人： Kevin T. Chapman ,  Daniele Malleo ,  J. Tanner Nevill ,  Steven W. Short ,  Mark P. White ,  M. Jimena Loureiro

IPC分类号： B01L3/00 ,  B03C5/00 ,  B03C5/02 ,  G01N15/14 ,  G01N33/50 ,  G01N33/543 ,  G01N33/68

CPC分类号： B01L3/502761 ,  B03C5/005 ,  B03C5/026 ,  G01N15/1484 ,  G01N33/5023 ,  G01N33/5047 ,  G01N33/505 ,  G01N33/5052 ,  G01N33/54313 ,  G01N33/6854 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2400/0454 ,  B03C2201/26

摘要： Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.