公开(公告)号：US20240310782A1

公开(公告)日：2024-09-19

申请号：US18546095

申请日：2022-02-09

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Aydogan Ozcan ,  Yair Rivenson ,  Luzhe Huang ,  Tairan Liu

IPC: G03H1/08 ,  G03H1/00 ,  G03H1/04 ,  G06T5/50 ,  G06T5/60

CPC classification number: G03H1/0866 ,  G03H1/0005 ,  G03H1/0443 ,  G06T5/50 ,  G06T5/60 ,  G03H2001/005 ,  G03H2001/0458 ,  G03H2001/0883 ,  G03H2210/55 ,  G06T2207/10056 ,  G06T2207/20084 ,  G06T2207/30024

Abstract: Digital holography is one of the most widely used label-free microscopy techniques in biomedical imaging. Recovery of the missing phase information of a hologram is an important step in holographic image reconstruction. A convolutional recurrent neural network (RNN)-based phase recovery approach is employed that uses multiple holograms, captured at different sample-to-sensor distances to rapidly reconstruct the phase and amplitude information of a sample, while also performing autofocusing through the same trained neural network. The success of this deep learning-enabled holography method is demonstrated by imaging microscopic features of human tissue samples and Papanicolaou (Pap) smears. These results constitute the first demonstration of the use of recurrent neural networks for holographic imaging and phase recovery, and compared with existing methods, the presented approach improves the reconstructed image quality, while also increasing the depth-of-field and inference speed.

公开(公告)号：US12038370B2

公开(公告)日：2024-07-16

申请号：US17621979

申请日：2020-07-02

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Aydogan Ozcan ,  Aniruddha Ray ,  Yibo Zhang ,  Dino Di Carlo

IPC: G01N15/1433 ,  B03C1/01 ,  B03C1/02 ,  G01N15/1434 ,  G03H1/00 ,  G06V10/147 ,  G06V10/82 ,  G06V20/69 ,  G01N15/01 ,  G01N15/10

CPC classification number: G01N15/1433 ,  B03C1/01 ,  B03C1/02 ,  G01N15/1434 ,  G03H1/0005 ,  G06V10/147 ,  G06V10/82 ,  G06V20/693 ,  G06V20/698 ,  B03C2201/18 ,  B03C2201/26 ,  G01N15/01 ,  G01N2015/1006 ,  G03H2001/005 ,  G03H2222/12

Abstract: A computational cytometer operates using magnetically modulated lensless speckle imaging, which introduces oscillatory motion to magnetic bead-conjugated rare cells of interest through a periodic magnetic force and uses lensless time-resolved holographic speckle imaging to rapidly detect the target cells in three-dimensions (3D). Detection specificity is further enhanced through a deep learning-based classifier that is based on a densely connected pseudo-3D convolutional neural network (P3D CNN), which automatically detects rare cells of interest based on their spatio-temporal features under a controlled magnetic force. This compact, cost-effective and high-throughput computational cytometer can be used for rare cell detection and quantification in bodily fluids for a variety of biomedical applications.

公开(公告)号：US20240168270A1

公开(公告)日：2024-05-23

申请号：US18315947

申请日：2023-05-11

Applicant: Korea Photonics Technology Institute

Inventor： Seon Kyu YOON ,  Jin Su Lee ,  Sung Kuk CHUN

IPC: G02B21/04 ,  G02B21/36 ,  G03H1/00

CPC classification number: G02B21/04 ,  G02B21/365 ,  G03H1/0005 ,  G03H2001/005

Abstract: This disclosure relates to a digital holographic microscope which is robust to an external environment. According to an aspect of the present embodiment, there is provided an optical system for a digital holographic microscope, the optical system including a beam splitter which reflects a light radiated from a light source toward an object, or passes a light reflected from and traveling from the object; an objective lens focusing the light reflected by the beam splitter on the object; a transflective mirror which is located on the surface of the objective lens and is determined to be transparent or reflective depending on a polarization direction of a light incident to the transflective mirror; and a wave plate which converts the light passing through the beam splitter into a circularly polarized light.

公开(公告)号：US20240126206A1

公开(公告)日：2024-04-18

申请号：US18533186

申请日：2023-12-08

Applicant: Korea Photonics Technology Institute

Inventor： Seon Kyu YOON ,  Ha Mong SHIM ,  Jin Su LEE ,  Kwang Hoon LEE

IPC: G03H1/00 ,  G01B9/021 ,  G01B11/16 ,  G03H1/04 ,  G03H1/08

CPC classification number: G03H1/0005 ,  G01B9/021 ,  G01B11/164 ,  G03H1/0402 ,  G03H1/0443 ,  G03H1/0866 ,  G03H2001/005 ,  G03H2001/0415

Abstract: According to an embodiment, a holographic microscope comprises a light source, an optical system splitting light emitted from the light source into an object and a reflective mirror and inducing interference between light reflected by the object or transmitted through the object and light reflected by the reflective mirror, a first image sensor receiving the interference light and sensing interference information for the interference light, a second image sensor receiving the light reflected by the object or transmitted through the object and sensing information for the received light, and an image processor deriving a shape of the object based on the interference information sensed by the first image sensor and the information sensed by the second image sensor.

公开(公告)号：US11948302B2

公开(公告)日：2024-04-02

申请号：US17196895

申请日：2021-03-09

Applicant: NEW YORK UNIVERSITY ,  SPHERYX, INC.

Inventor： David G. Grier ,  Fook Chiong Cheong ,  Kaitlynn Snyder ,  Rushna Quddus ,  Lauren E. Altman ,  Kent Kirshenbaum

IPC: G06T7/00 ,  G01N15/1434 ,  G02B21/36 ,  G03H1/00 ,  G03H1/04 ,  G06T7/62

CPC classification number: G06T7/0014 ,  G01N15/1434 ,  G02B21/365 ,  G03H1/0005 ,  G03H1/0465 ,  G06T7/62 ,  G01N2015/1445 ,  G03H2001/005 ,  G03H2001/0471 ,  G03H2210/55 ,  G03H2222/13 ,  G03H2222/34 ,  G06T2207/10056 ,  G06T2207/30024

Abstract: An in-line holographic microscope can be used to analyze a video stream to track individual colloidal particles' three-dimensional motions. The system and method can provide real time nanometer resolution, and simultaneously measure particle sizes and refractive indexes. An assay using the holographic microscope for holographic particle characterization directly detect viruses, antibodies and related targets binding to the surfaces of specifically functionalized micrometer-scale colloidal probe beads. The system detects binding of targets by directly measuring associated changes in the bead's diameter without the need for downstream labeling and analysis.

公开(公告)号：US20230213425A1

公开(公告)日：2023-07-06

申请号：US18148499

申请日：2022-12-30

Applicant: NEW YORK UNIVERSITY ,  SPHERYX, INC.

Inventor： David G. GRIER ,  Mary Ann ODETE ,  Fook Chiong CHEONG ,  Annemarie WINTERS ,  Jesse J. ELLIOTT ,  Laura A. PHILIPS

IPC: G01N15/08 ,  G03H1/00 ,  G03H1/04 ,  G06T7/62

CPC classification number: G01N15/08 ,  G03H1/0005 ,  G03H1/0443 ,  G06T7/62 ,  G01N2015/0846 ,  G03H2001/005 ,  G03H2210/55 ,  G06T2207/10016 ,  G06T2207/10056

Abstract: Holographic Video Microscopy analysis of non-spherical particles is disclosed herein. Properties of the particles are determined by application of light scattering theory to holography data. Effective sphere theory is applied to provide information regarding the reflective index of a sphere that includes a target particle. Known particles may be co-dispersed with unknown particles in a medium and the holographic video microscopy is used to determine properties, such as porosity, of the unknown particles.

公开(公告)号：US11687031B2

公开(公告)日：2023-06-27

申请号：US17128092

申请日：2020-12-19

Applicant: IMEC VZW ,  Katholieke Universiteit Leuven

Inventor： Zhenxiang Luo ,  Abdulkadir Yurt ,  Dries Braeken ,  Liesbet Lagae ,  Richard Stahl

IPC: G03H1/04 ,  G03H1/00 ,  H04N13/254 ,  G02B21/06 ,  G02B21/36

CPC classification number: G03H1/0005 ,  G02B21/06 ,  G02B21/367 ,  G03H1/0443 ,  G03H1/0465 ,  H04N13/254 ,  G03H2001/005 ,  G03H2001/0471 ,  G03H2210/30 ,  G03H2210/55 ,  G03H2222/34 ,  G03H2226/02 ,  G03H2226/11

Abstract: A method for three-dimensional imaging of a sample (302) comprises: receiving (102) interference patterns (208) acquired using light-detecting elements (212), wherein each interference pattern (208) is formed by scattered light from the sample (302) and non-scattered light from a light source (206; 306), wherein the interference patterns (208) are acquired using different angles between the sample (302) and the light source (206; 306); performing digital holographic reconstruction applying an iterative algorithm to change a three-dimensional scattering potential of the sample (302) to improve a difference between the received interference patterns (208) and predicted interference patterns based on the three-dimensional scattering potential; wherein the iterative algorithm reduces a sum of a data fidelity term and a non-differentiable regularization term and wherein the iterative algorithm includes a forward-backward splitting method alternating between forward gradient descent (108) on the data fidelity term and backward gradient descent (110) on the regularization term.

公开(公告)号：US10082662B2

公开(公告)日：2018-09-25

申请号：US15243265

申请日：2016-08-22

Applicant: Korea Advanced Institute of Science and Technology ,  Tomocube, Inc.

Inventor： YongKeun Park ,  Seungwoo Shin ,  Gwang Sik Park

IPC: G02B21/36 ,  G01N21/41 ,  G01N21/64 ,  G02B21/16 ,  G02B26/08 ,  G02B27/46 ,  G03H1/00 ,  G01N21/45 ,  G01N21/17

CPC classification number: G02B21/367 ,  G01N21/4133 ,  G01N21/45 ,  G01N21/6458 ,  G01N21/6486 ,  G01N2021/1787 ,  G01N2201/0635 ,  G02B21/16 ,  G02B26/0833 ,  G02B27/46 ,  G03H1/0005 ,  G03H2001/005

Abstract: An ultra-high-speed 3D refractive index tomography and structured illumination microscopy system using a wavefront shaper and a method using the same are provided. A method of using an ultra-high-speed 3D refractive index tomography and structured illumination microscopy system that utilizes a wavefront shaper includes adjusting an irradiation angle of a plane wave incident on a sample by using the wavefront shaper, measuring a 2D optical field, which passes through the sample, based on the irradiation angle of the plane wave, and obtaining a 3D refractive index image from information of the measured 2D optical field by using an optical diffraction tomography or a filtered back projection algorithm.

公开(公告)号：US20180231753A1

公开(公告)日：2018-08-16

申请号：US15943595

申请日：2018-04-02

Applicant: Alentic Microscience Inc.

Inventor： Alan Marc Fine

IPC: G02B21/00 ,  G02B21/36 ,  G01N33/80 ,  G01N15/14 ,  G06K9/00 ,  G01T1/02 ,  G01N11/00 ,  G03H1/00 ,  G01B9/02 ,  G03H1/04 ,  G01N15/00 ,  C12M1/34 ,  G01N15/10 ,  G06T7/00

CPC classification number: G02B21/0008 ,  C12M41/36 ,  G01B9/02041 ,  G01N15/1429 ,  G01N15/1434 ,  G01N15/1463 ,  G01N15/1475 ,  G01N33/80 ,  G01N2011/008 ,  G01N2015/0069 ,  G01N2015/1006 ,  G01N2015/1486 ,  G01N2015/149 ,  G01T1/02 ,  G02B21/00 ,  G02B21/0004 ,  G02B21/361 ,  G02B21/365 ,  G03H2001/005 ,  G03H2001/045 ,  G06K9/00127 ,  G06K9/00147 ,  G06T7/0012 ,  G06T2207/10056 ,  G06T2207/30242 ,  Y10S435/808

Abstract: Among other things, a method comprises imaging a sample displaced between a sensor surface and a surface of a microscopy sample chamber to produce an image of at least a part of the sample. The image is produced using lensless optical microscopy, and the sample contains at least blood from a subject. The method also comprises automatically differentiating cells of different types in the image, generating a count of one or more cell types based on the automatic differentiation, and deriving a radiation dose the subject has absorbed based on the count.

公开(公告)号：US20180143587A1

公开(公告)日：2018-05-24

申请号：US15861115

申请日：2018-01-03

Applicant: CellOptic, Inc.

Inventor： Gary BROOKER ,  Nisan SIEGEL ,  Joseph ROSEN

IPC: G03H1/04 ,  G02B21/16 ,  G03H1/06 ,  G03H5/00 ,  G02B21/00 ,  G03H1/00

CPC classification number: G03H1/0443 ,  G02B21/0068 ,  G02B21/16 ,  G03H1/041 ,  G03H1/06 ,  G03H5/00 ,  G03H2001/005 ,  G03H2001/0447 ,  G03H2001/0452 ,  G03H2222/24 ,  G03H2222/31 ,  G03H2223/22 ,  G03H2225/22

Abstract: A new optical arrangement that creates high efficiency, high quality Fresnel Incoherent Correlation Holography (FINCH) holograms using transmission liquid crystal GRIN (TLCGRIN) diffractive lenses has been invented. This is in contrast to the universal practice in the field of using a reflective spatial light modulator (SLM) to separate sample and reference beams. Polarization sensitive TLCGRIN lenses enable a straight optical path, have 95% transmission efficiency, are analog devices without pixels and are free of many limitations of reflective SLM devices. An additional advantage is that they create an incoherent holographic system that is achromatic over a wide bandwidth. Two spherical beams created by the combination of a glass and a polarization sensitive TLCGRIN lenses interfere and a hologram is recorded by a digital camera. FINCH configurations which increase signal to noise ratios and imaging speed are also described.